RESUMO
Morphological features are essential to assess neonates' viability. In order to characterize the morphology and biometry of newborn agouti, 36 animals born at 103 days of gestation under human care in Teresina-PI, Brazil were evaluated. After birth, the newborns were weighed on digital precision scales, measured with a flexible tape measure and their external morphology was assessed. The animals observed had coat color trait of adults, open eyes, hairs in the nasal region and four incisor teeth. The forelimbs had developed four digits and the hind limbs three digits, with white edged claws and a short hairless tail. The following were observed: weight of 144.58 ± 33.39g and lengths: crown-rump 14.73 ± 1.82cm, total 19.88 ± 1.52cm, head to tail 16.95 ± 1.55cm, head 7.33 ± 0.59cm, thoracic girth 11.78 ± 1.36cm, abdominal girth 10.73 ± 1.21cm, tail length 1.18 ± 0.25cm, eye diameter 1.27 ± 0.27cm and ear length 0.21 ± 0.35cm. The agoutis presented morphological maturity at birth with external morphology very similar to that of the adult animals. Most of the births in this species are the double type and newborns that weigh more than 90g have better chances of survival in captivity.
As características morfológicas são essenciais para avaliar a viabilidade dos neonatos. Para caracterizar a morfologia e biometria de cutias recém-nascidas, foram avaliados 36 animais nascidos aos 103 dias de gestação sob cuidados em Teresina-PI, Brasil. Após o nascimento, os recém-nascidos foram pesados em balança digital de precisão, medidos com fita métrica flexível e avaliada sua morfologia externa. Os animais observados apresentavam traço de pelagem de adultos, olhos abertos, pelos na região nasal e quatro dentes incisivos. Os membros anteriores desenvolveram quatro dedos e os membros posteriores três, com garras brancas afiadas e uma cauda curta e sem pêlos. Foram observados: peso de 144,58 ± 33,39g e comprimentos: garupa 14,73 ± 1,82cm, total 19,88 ± 1,52cm, cabeça a cauda 16,95 ± 1,55cm, cabeça 7,33 ± 0,59cm, perímetro torácico 11,78 ± 1,36cm, abdominal perímetro 10,73 ± 1,21 cm, comprimento da cauda 1,18 ± 0,25 cm, diâmetro do olho 1,27 ± 0,27 cm e comprimento da orelha 0,21 ± 0,35 cm. As cutias apresentaram maturidade morfológica ao nascimento com morfologia externa muito semelhante à dos animais adultos. A maioria dos nascimentos nesta espécie são do tipo duplo e recém-nascidos que pesam mais de 90g têm melhores chances de sobrevivência sob cuidados humanos.
Assuntos
Animais , Pesos e Medidas Corporais/veterinária , Estruturas Animais/crescimento & desenvolvimento , Dasyproctidae/crescimento & desenvolvimento , Anatomia Veterinária , Sistemas de Identificação Animal , Animais Recém-Nascidos/anatomia & histologiaRESUMO
The aim of this estudy was to establish the levels of serum total protein, albumin, globulin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), calcium, phosphorus, urea, creatinine, bilirubin and glucose during pregnancy in agoutis. Animals: Twelve pregnant agouti from the Center for the Study and Preservation of Wild Animals (CSPWA) of the Federal University of Piauí (UFPI) were used in this research. After identification of the estrus, the day of the coverage was confirmed by means of vaginal cytology with the visualization of spermatozoa (day zero) and confirmation of pregnancy by ultrasonographic examination after 15 days. Blood samples were collected by lateral saphenous vein puncture after physical restraint, every 10 days until the end of pregnancy, for biochemical analyzes. A completely randomized experimental design was used and the means compared by the Duncan test at 5% probability using the SAS (Statistical Analysis System). The results of the biochemical analysis of total protein, albumin, globulin, urea, creatinine, calcium, phosphorus, serum ALT, glucose, AST, total bilirubin, direct bilirubin and indirect bilirubin in pregnant agouti (Dasyprocta prymnolopha) did not differ when compared to nonpregnant females. The serum biochemical levels during pregnancy in agoutis, except for calcium and phosphorus, were unchanged compared to those found in the non-pregnant adult animal, as occurs in other species. The changes during pregnancy reflect the physiology and biology of wild species, elucidating information about the biochemical parameters during pregnancy, thus characterizing the animal as a benchmark for comparisons with other species, extolling its importance both for nature conservation and production in capivity.
O estudo objetivou estabelecer os níveis séricos de proteínas totais, albumina, globulina, Alanina Aminotransferase (ALT), Aspartato Aminotransferase (AST), cálcio, fósforo, ureia, creatinina, bilirrubina e glicose durante a gestação em cutias. A pesquisa foi desenvolvida utilizando-se 12 cutias fêmeas criadas no Núcleo de Estudos, Produção e Preservação de Animais Silvestres da Universidade Federal do Piauí. Após a identificação do estro, o dia da cobertura foi confirmado por meio de citologia vaginal com a visualização de espermatozoides (dia zero) e confirmação da gestação por exame ultrassonográfico após 15 dias. Confirmada a gestação, foram coletados 03 mL de sangue mediante punção da veia pudenda interna, após contenção física, a cada 10 dias, até o final da gestação. Foi feita a análise de variância para um delineamento inteiramente casualizado com teste de Duncan para comparação das médias a 5% de probabilidade utilizando-se do programa estatístico SAS (Statistical Analysis System). Os resultados obtidos por meio da análise bioquímica de proteína total, albumina, globulina, ureia, creatinina, cálcio, fósforo, ALT séricas, glicose, aspartato aminotransferase (AST), bilirrubina total, bilirrubina direta e bilirrubina indireta de cutias gestantes (Dasyprocta prymnolopha) diferem de forma absoluta quando comparados a fêmeas não gestantes. Os níveis bioquímicos séricos durante a gestação em cutias, com exceção do cálcio, fósforo, sofrem alterações comparadas ao animal adulto não prenhe, como ocorre em outras espécies. Os níveis nas cutias gestantes sofrem alterações de acordo com o tempo de gestação, com maiores mudanças no período inicial e final da prenhez. As mudanças durante a gravidez refletem a fisiologia e a biologia da espécie silvestre, elucidando informações sobre os parâmetros bioquímicos durante a gestação, caracterizando o animal como referência para comparações com outras espécies, exaltando a importância tanto para sua conservação quanto para a sua produção em cativeiro.
Assuntos
Aspartato Aminotransferases , Alanina Transaminase , Albuminas , Dasyproctidae , Globulinas , GravidezRESUMO
Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...](AU)
Assuntos
Animais , Artiodáctilos , Células-Tronco Mesenquimais , Células da Medula Óssea , Terapia Baseada em Transplante de Células e Tecidos/veterinária , Separação Celular/normas , Adipogenia , Criopreservação/veterinária , Modelos AnimaisRESUMO
Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...]
Assuntos
Animais , Artiodáctilos , Células da Medula Óssea , Células-Tronco Mesenquimais , Adipogenia , Criopreservação/veterinária , Modelos Animais , Separação Celular/normas , Terapia Baseada em Transplante de Células e Tecidos/veterináriaRESUMO
Knowledge of wild species embryonic development is important for their maintenance in captivity or the wild. The objective of the present study was to characterize the external morphology and define the biometry of greater rhea embryos and fetuses at different stages of development. A total of 41 embryos and fetuses were analyzed to describe their external morphology using a stereoscopic microscope. The crown-rump (CR), total length (TL), cephalocaudal length (CCL), biparietal diameter (BPD), beak, humerus and tibio-tarsal lengths were measured by digital pachymeter, millimetric scale ruler and cotton thread. The weight of the embryos and fetuses was measured on digital scales. The greater rhea embryos at 5, 6 and 7 days incubation presented a "C" shape. At 9, 10 and 11 days the eyes were big and pigmented. At 11, 12 and 13 days the eyelid covered more than half the eye, resulting in an oval slit. In 14 and 15 day-old embryos, the skin was still thin and the ribs evident, but at 18 days this structure was thicker. In embryos at 21 and 27 days of development closed eyelids were observed forming an eyelid slit, and the eye ball was less pronounced at 27 days. Weight gain presented an exponential growth curve, while measurements such as TL, DBP, beak, humerus and tibio-tarsal length had linear growth over time. Thus it was possible to characterize the greater rhea embryos and fetuses at several incubation ages using their external morphology and morphometric analyses.
Assuntos
Reiformes/embriologia , Animais , Biometria , Embrião não Mamífero/anatomia & histologia , Desenvolvimento EmbrionárioRESUMO
PURPOSE: The use of the collared peccary as an experimental model for ischemic nephropathy. METHODS: A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery. Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology. RESULTS: Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion. CONCLUSION: The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.
Assuntos
Modelos Animais de Doenças , Isquemia/etiologia , Rim/irrigação sanguínea , Obstrução da Artéria Renal/etiologia , Animais , Constrição , Rim/diagnóstico por imagem , Rim/patologia , Rim/cirurgia , Masculino , Necrose , Nefrite/etiologia , Nefrite/patologia , Valores de Referência , Artéria Renal/patologia , Artéria Renal/cirurgia , Obstrução da Artéria Renal/patologia , Reprodutibilidade dos Testes , Suínos , Fatores de Tempo , UltrassonografiaRESUMO
PURPOSE: The use of the collared peccary as an experimental model for ischemic nephropathy. METHODS: A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery. Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology. RESULTS: Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion. CONCLUSION: The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy. .
Assuntos
Animais , Masculino , Modelos Animais de Doenças , Isquemia/etiologia , Rim/irrigação sanguínea , Obstrução da Artéria Renal/etiologia , Constrição , Rim/patologia , Rim/cirurgia , Rim , Necrose , Nefrite/etiologia , Nefrite/patologia , Valores de Referência , Reprodutibilidade dos Testes , Obstrução da Artéria Renal/patologia , Artéria Renal/patologia , Artéria Renal/cirurgia , Suínos , Fatores de TempoRESUMO
PURPOSE:The use of the collared peccary as an experimental model for ischemic nephropathy.METHODS:A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery. Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology.RESULTS:Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion.CONCLUSION:The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.(AU)
Assuntos
Animais , Artiodáctilos/anatomia & histologia , Nefropatias/veterinária , Isquemia/veterinária , Artéria Renal , Modelos Animais de DoençasRESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.
Assuntos
Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Animais , Brasil , Técnicas de Cultura de Células , Diferenciação Celular , Proliferação de Células , Feminino , Citometria de Fluxo/métodos , Cabras , Masculino , Modelos Animais , Fator 3 de Transcrição de Octâmero/isolamento & purificação , Antígeno Nuclear de Célula em Proliferação/isolamento & purificação , Vimentina/isolamento & purificaçãoRESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied. .
Assuntos
Animais , Feminino , Masculino , Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Brasil , Técnicas de Cultura de Células , Diferenciação Celular , Proliferação de Células , Citometria de Fluxo/métodos , Cabras , Modelos Animais , /isolamento & purificação , Antígeno Nuclear de Célula em Proliferação/isolamento & purificação , Vimentina/isolamento & purificaçãoRESUMO
Non-human primates serve as a model for studies on the dynamics of transmission routes and natural history of diseases shared between humans and animals. The captive environment is conducive the dissemination of zoonotic diseases. Many of these animals do not present clinical symptomatology, even when infected, which characterize them as an important source of infection for domestic animals and man. The objective of this study was to evaluate the sanity of non-human primates kept in captivity through hematological and biochemical analysis as well as peripheral blood smear, aiming to investigate the presence of zoonotic pathogens, serving as a model for future studies on the dynamics of routes of transmission of diseases shared between humans and animals. Were collected samples of blood from 15 nail monkeys (Cebus sp.), adults, clinically healthy and belonging to Park Zoobotanic of Teresina. Were stained smear sanguine blades and obtained the haematological and biochemical profiles of each animal. The data analysis was based on basic statistics. Did not observed any haemoparasite present in peripheral blood. All animals were anemic, 46,7% thrombopenics and 87% of the animals showed some type of pathological process of chronic evolution, due to the high rate of monocytes found. All animals showed high rates of alkaline phosphatase, and transaminases AST and ALT, indicating injury of the hepatic parenchyma. New studies should be conducted to better elucidate of results, seeing that biochemical physiological data primate of the genus Cebus are scarce in literature.(AU)
Os primatas não humanos atuam como modelo para estudos sobre a dinâmica das vias de transmissão e história natural de doenças compartilhadas entre homens e animais. O ambiente de cativeiro é propício à disseminação de doenças de caráter zoonótico. Muitos destes animais não apresentam sintomatologia clínica, mesmo estando infectados, o que os caracterizam como importante fonte de infecção para os animais domésticos e o homem. O objetivo deste trabalho foi avaliar a sanidade de primatas não humanos mantidos em cativeiro através de análise hematológica e bioquímica, bem como esfregaço de sangue periférico, com intuito de investigar a presença de patógenos de caráter zoonótico, servindo de modelo para estudos futuros sobre a dinâmica das vias de transmissão de doenças compartilhadas entre homens e animais. Foram coletadas amostras de sangue de 15 macacos prego (Cebus sp.), adultos, clinicamente saudáveis e pertencentes ao Parque Zoobotânico de Teresina. Foram coradas lâminas de esfregaço sanguíneo e obtidos os perfis hematológicos e bioquímicos de cada animal. A análise dos dados baseou-se em estatística básica. Não foi observado nenhum hemoparasita presente em sangue periférico. Todos os animais estavam anêmicos, 46,7% trombopênicos e 87% apresentavam algum tipo de processo patológico de evolução crônica, devido à elevada taxa de monócitos encontrados. Todos os animais apresentaram elevadas taxas de fosfatase alcalina e das transaminases AST e ALT, indicando injúria do parênquima hepático. Novos estudos deverão ser realizados para melhor elucidação dos resultados, visto que dados bioquímicos fisiológicos de primatas do gênero Cebus são escassos na literatura.(AU)
Assuntos
Animais , Cebus/sangue , Anemia , Fosfatase Alcalina , Transaminases , Trombocitopenia , Testes Hematológicos/veterinária , ZoonosesRESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.(AU)
Assuntos
Animais , Cabras , Medula Óssea , Células-Tronco/classificação , Pesquisa com Células-Tronco , Citometria de Fluxo/veterinária , Técnicas de Cultura de Células , Antígeno Nuclear de Célula em Proliferação/análiseRESUMO
Non-human primates serve as a model for studies on the dynamics of transmission routes and natural history of diseases shared between humans and animals. The captive environment is conducive the dissemination of zoonotic diseases. Many of these animals do not present clinical symptomatology, even when infected, which characterize them as an important source of infection for domestic animals and man. The objective of this study was to evaluate the sanity of non-human primates kept in captivity through hematological and biochemical analysis as well as peripheral blood smear, aiming to investigate the presence of zoonotic pathogens, serving as a model for future studies on the dynamics of routes of transmission of diseases shared between humans and animals. Were collected samples of blood from 15 nail monkeys (Cebus sp.), adults, clinically healthy and belonging to Park Zoobotanic of Teresina. Were stained smear sanguine blades and obtained the haematological and biochemical profiles of each animal. The data analysis was based on basic statistics. Did not observed any haemoparasite present in peripheral blood. All animals were anemic, 46,7% thrombopenics and 87% of the animals showed some type of pathological process of chronic evolution, due to the high rate of monocytes found. All animals showed high rates of alkaline phosphatase, and transaminases AST and ALT, indicating injury of the hepatic parenchyma. New studies should be conducted to better elucidate of results, seeing that biochemical physiological data primate of the genus Cebus are scarce in literature.
Os primatas não humanos atuam como modelo para estudos sobre a dinâmica das vias de transmissão e história natural de doenças compartilhadas entre homens e animais. O ambiente de cativeiro é propício à disseminação de doenças de caráter zoonótico. Muitos destes animais não apresentam sintomatologia clínica, mesmo estando infectados, o que os caracterizam como importante fonte de infecção para os animais domésticos e o homem. O objetivo deste trabalho foi avaliar a sanidade de primatas não humanos mantidos em cativeiro através de análise hematológica e bioquímica, bem como esfregaço de sangue periférico, com intuito de investigar a presença de patógenos de caráter zoonótico, servindo de modelo para estudos futuros sobre a dinâmica das vias de transmissão de doenças compartilhadas entre homens e animais. Foram coletadas amostras de sangue de 15 macacos prego (Cebus sp.), adultos, clinicamente saudáveis e pertencentes ao Parque Zoobotânico de Teresina. Foram coradas lâminas de esfregaço sanguíneo e obtidos os perfis hematológicos e bioquímicos de cada animal. A análise dos dados baseou-se em estatística básica. Não foi observado nenhum hemoparasita presente em sangue periférico. Todos os animais estavam anêmicos, 46,7% trombopênicos e 87% apresentavam algum tipo de processo patológico de evolução crônica, devido à elevada taxa de monócitos encontrados. Todos os animais apresentaram elevadas taxas de fosfatase alcalina e das transaminases AST e ALT, indicando injúria do parênquima hepático. Novos estudos deverão ser realizados para melhor elucidação dos resultados, visto que dados bioquímicos fisiológicos de primatas do gênero Cebus são escassos na literatura.
Assuntos
Animais , Anemia , Cebus/sangue , Fosfatase Alcalina , Transaminases , Trombocitopenia , Testes Hematológicos/veterinária , ZoonosesRESUMO
The gestation period in agoutis can range from 104 to 120 days. Knowledge regarding the morphological characteristics of embryos and fetuses is important as a base for studies in reproduction biotechnology, such as in vitro fertilization, embryo transfer and helps in determining congenital anomalies during the development phase. Thus, given the importance and lack of information about agouti embryology, the objective of this study was to characterize the external morphology and define the biometry of embryos and fetuses, at different days of development. Nine females were submitted to daily colpocytology to identify the estrus, confirm mating and identify day zero of the gestation. When the mating was confirmed they were weighed, underwent abdominal ultrasonography and surgery was conducted on the females at the gestational ages of 25, 30, 35, 40, 45, 50, 75 and 100 days. Sixteen embryos/fetuses were weighed and measured. Agouti embryos at 25 days after mating are "C" shaped, with primitive structures, 0.4±0.01cm crown-rump and weighed 0.06±0.01g; at 30 days after mating the crown-rump was 0.95±0.07cm and weighed 0.28±0.00g; at 35 days after mating the crown-rump was 155±0.07cm and weighed 0.38±0.01g; at 40 days after mating the crown-rump was 2.25±0.21cm and weighed 1.25±0.07g; at 45 days after mating the crown-rump was 3.45±0.35cm and weighed 2.75±0.64g; at 50 days after mating the crown-rump was 5.0±0.3cm and weighed 7.01±2.6g; at 75 days after mating, the skin was dark, the crown-rump was 10.0±0.14cm and weighed 55.2±0.07g. At 100 days after mating, the crown-rump was 13.8±0.49cm and fetuses weighed 136.7±9.40g. Based on the morphological data assessed the embryo and fetus age could be assessed and the size and average weight of agouti embryos was established.
Assuntos
Desenvolvimento Fetal/fisiologia , Feto/fisiologia , Roedores/embriologia , Animais , Estatura Cabeça-Cóccix , Feminino , Gravidez , Ultrassonografia Pré-Natal/veterináriaRESUMO
Stem cells are present in the adult tissues of most diverse species. Bone marrow is recognized to be the most exploited site to obtain stem cells and cell progenitors. The objective of the present study was to characterize hematopoietic progenitor (HP) morphology and analyze the performance of adherent cell progenitors (ACPs) cultivated in vitro from black-rumped agouti bone marrow (Dasyprocta prymnolopha). Bone marrow aspirates were obtained from tibia crest and used to prepare histological slides and identify cell morphology. Cells were also scattered on culture plates for later isolation, expansion, and quantification. Smears obtained from bone marrow demonstrated HPs at different stages of maturity. In culture, these cells showed fibroblastoid morphology and a strong tendency to form colonies, demonstrated by the presence of cell aggregates, cytoplasmic elongations lying side by side. An 80% cell confluence was observed at 18 days in culture and progressive reduction in the percentage of nonadherent mononuclear cells. After eight passes, a mean cell viability of 96.07% was observed, from a pool of 1.6 × 10(7) cells (ACP). Thirteen 25-cm(2) culture bottles were trypsinized, resuspended in freezing medium, stored in 14 criotubes at a concentration of 1 × 10(6) cells per milliliter, and placed in liquid nitrogen at -196°C. Agouti bone marrow demonstrated high plasticity, moreover different HP lines, and a population of adherent cells demonstrated morphology similar to mesenchymal stem cells in culture.