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Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil
Silva Filho, Osmar Ferreira da; Argôlo Neto, Napoleão Martins; Carvalho, Maria Acelina Martins de; Carvalho, Yulla Klinger de; Diniz, Anaemilia das Neves; Moura, Laécio da Silva; Ambrósio, Carlos Eduardo; Monteiro, Janaína Munuera; Almeida, Hatawa Melo de; Miglino, Maria Angélica; Alves, Jacyara de Jesus Rosa Pereira; Macedo, Kássio Vieira; Rocha, Andressa Rego da; Feitosa, Matheus Levi Tajra; Alves, Flávio Ribeiro.
Afiliação
  • Silva Filho, Osmar Ferreira da; UFPI. School of Veterinary Medicine. Teresina. BR
  • Argôlo Neto, Napoleão Martins; UFPI. School of Veterinary Medicine. Teresina. BR
  • Carvalho, Maria Acelina Martins de; UFPI. School of Veterinary Medicine. Teresina. BR
  • Carvalho, Yulla Klinger de; UFPI. School of Veterinary Medicine. Teresina. BR
  • Diniz, Anaemilia das Neves; UFPI. School of Veterinary Medicine. Teresina. BR
  • Moura, Laécio da Silva; UFPI. School of Veterinary Medicine. Teresina. BR
  • Ambrósio, Carlos Eduardo; UFPI. School of Veterinary Medicine. Teresina. BR
  • Monteiro, Janaína Munuera; UFPI. School of Veterinary Medicine. Teresina. BR
  • Almeida, Hatawa Melo de; UFPI. School of Veterinary Medicine. Teresina. BR
  • Miglino, Maria Angélica; UFPI. School of Veterinary Medicine. Teresina. BR
  • Alves, Jacyara de Jesus Rosa Pereira; UFPI. School of Veterinary Medicine. Teresina. BR
  • Macedo, Kássio Vieira; UFPI. School of Veterinary Medicine. Teresina. BR
  • Rocha, Andressa Rego da; UFPI. School of Veterinary Medicine. Teresina. BR
  • Feitosa, Matheus Levi Tajra; UFPI. School of Veterinary Medicine. Teresina. BR
  • Alves, Flávio Ribeiro; UFPI. School of Veterinary Medicine. Teresina. BR
Acta cir. bras ; Acta cir. bras;29(8): 478-484, 08/2014. graf
Article em En | LILACS | ID: lil-719180
Biblioteca responsável: BR1.1
ABSTRACT

PURPOSE:

To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model.

METHODS:

Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog).

RESULTS:

Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog.

CONCLUSIONS:

Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied. .
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Células da Medula Óssea / Células-Tronco Mesenquimais Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Células da Medula Óssea / Células-Tronco Mesenquimais Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil