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Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil.
Silva Filho, Osmar Ferreira da; Argôlo Neto, Napoleão Martins; Carvalho, Maria Acelina Martins de; Carvalho, Yulla Klinger de; Diniz, Anaemilia das Neves; Moura, Laécio da Silva; Ambrósio, Carlos Eduardo; Monteiro, Janaína Munuera; Almeida, Hatawa Melo de; Miglino, Maria Angélica; Alves, Jacyara de Jesus Rosa Pereira; Macedo, Kássio Vieira; Rocha, Andressa Rego da; Feitosa, Matheus Levi Tajra; Alves, Flávio Ribeiro.
Afiliação
  • Silva Filho OF; School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
  • Argôlo Neto NM; Department of Animal Science, School of Veterinary Medicine, UFPI, Bom Jesus, PI, Brazil.
  • Carvalho MA; Department of Veterinary Morphophysiology, School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
  • Carvalho YK; UFPI, Teresina, PI, Brazil.
  • Diniz Ad; Department of Animal Science, State University of Piaui, Uniao, PI, Brazil.
  • Moura Lda S; School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
  • Ambrósio CE; Department of Basic Sciences, Faculty of Animal Sciences and Food Engineering, Sao Paulo University, Pirassununga, SP, Brazil.
  • Monteiro JM; Teaching and Research Institute, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
  • Almeida HM; Department of Animal Science, UESPI, Uniao, PI, Brazil.
  • Miglino MA; Department of Surgery, School of Veterinary, USP, Sao Paulo, SP, Brazil.
  • Alves Jde J; Federal University of Ceara, Fortaleza, CE, Brazil.
  • Macedo KV; UFPI, Teresina, PI, Brazil.
  • Rocha AR; School of Veterinary Medicine, UFPI, Teresina, PI, Brazil.
  • Feitosa ML; UFPI, Teresina, PI, Brazil.
  • Alves FR; Department of Veterinary Morphophysiology, UFPI, Teresina, PI, Brazil.
Acta Cir Bras ; 29(8): 478-84, 2014 Aug.
Article em En | MEDLINE | ID: mdl-25140588
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta Cir Bras Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Acta Cir Bras Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil