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Facial expressions can convey the internal emotions of a person within a certain scenario and play a major role in the social interaction of human beings. In automatic Facial Expression Recognition (FER) systems, the method applied for feature extraction plays a major role in determining the performance of a system. In this regard, by drawing inspiration from the Swastik symbol, three texture based feature descriptors named Symbol Patterns (SP1, SP2 and SP3) have been proposed for facial feature extraction. SP1 generates one pattern value by comparing eight pixels within a 3×3 neighborhood, whereas, SP2 and SP3 generates two pattern values each by comparing twelve and sixteen pixels within a 5×5 neighborhood respectively. In this work, the proposed Symbol Patterns (SP) have been evaluated with natural, fibonacci, odd, prime, squares and binary weights for determining the optimal recognition accuracy. The proposed SP methods have been tested on MUG, TFEID, CK+, KDEF, FER2013 and FERG datasets and the results from the experimental analysis demonstrated an improvement in the recognition accuracy when compared to the existing FER methods.

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Successful treatment of pulmonary tuberculosis (TB) depends on early diagnosis and careful monitoring of treatment response. Identification of acid-fast bacilli by fluorescence microscopy of sputum smears is a common tool for both tasks. Microscopy-based analysis of the intracellular lipid content and dimensions of individual Mycobacterium tuberculosis (Mtb) cells also describe phenotypic changes which may improve our biological understanding of antibiotic therapy for TB. However, fluorescence microscopy is a challenging, time-consuming and subjective procedure. In this work, we automate examination of fields of view (FOVs) from microscopy images to determine the lipid content and dimensions (length and width) of Mtb cells. We introduce an adapted variation of the UNet model to efficiently localising bacteria within FOVs stained by two fluorescence dyes; auramine O to identify Mtb and LipidTox Red to identify intracellular lipids. Thereafter, we propose a feature extractor in conjunction with feature descriptors to extract a representation into a support vector multi-regressor and estimate the length and width of each bacterium. Using a real-world data corpus from Tanzania, the proposed method i) outperformed previous methods for bacterial detection with a 8% improvement (Dice coefficient) and ii) estimated the cell length and width with a root mean square error of less than 0.01%. Our network can be used to examine phenotypic characteristics of Mtb cells visualised by fluorescence microscopy, improving consistency and time efficiency of this procedure compared to manual methods.

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PURPOSE: In laparoscopic liver surgery, preoperative information can be overlaid onto the intra-operative scene by registering a 3D preoperative model to the intra-operative partial surface reconstructed from the laparoscopic video. To assist with this task, we explore the use of learning-based feature descriptors, which, to our best knowledge, have not been explored for use in laparoscopic liver registration. Furthermore, a dataset to train and evaluate the use of learning-based descriptors does not exist. METHODS: We present the LiverMatch dataset consisting of 16 preoperative models and their simulated intra-operative 3D surfaces. We also propose the LiverMatch network designed for this task, which outputs per-point feature descriptors, visibility scores, and matched points. RESULTS: We compare the proposed LiverMatch network with a network closest to LiverMatch and a histogram-based 3D descriptor on the testing split of the LiverMatch dataset, which includes two unseen preoperative models and 1400 intra-operative surfaces. Results suggest that our LiverMatch network can predict more accurate and dense matches than the other two methods and can be seamlessly integrated with a RANSAC-ICP-based registration algorithm to achieve an accurate initial alignment. CONCLUSION: The use of learning-based feature descriptors in laparoscopic liver registration (LLR) is promising, as it can help achieve an accurate initial rigid alignment, which, in turn, serves as an initialization for subsequent non-rigid registration.

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In a previous publication [1], we created a dataset of feature patches for detection model training. In this paper, we use the same patches to create a new large synthetic dataset of feature pairs, similar and different, in order to perform, thanks to a siamese convolutional model, the description and matching of the detected features. We thus complete the entire matching pipeline. The accurate manual labeling of image features being very difficult because of their large number and the various associated parameters of position, scale and rotation, recent deep learning models use the result of handcrafted methods for training. Compared to existing datasets, ours avoids model training with false detections of the extraction of feature patches by other algorithms, or with inaccuracy errors of manual labeling. The other advantage of synthetic patches is that we can control their content (corners, edges, etc.), as well as their geometric and photometric parameters, and therefore we control the invariance of the model. The proposed datasets thus allow a new approach to train the different matching modules without using traditional methods. To our knowledge, these are the first feature datasets based on generated synthetic patches for image matching.

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Palmprint recognition has received tremendous research interests due to its outstanding user-friendliness such as non-invasive and good hygiene properties. Most recent palmprint recognition studies such as deep-learning methods usually learn discriminative features from palmprint images, which usually require a large number of labeled samples to achieve a reasonable good recognition performance. However, palmprint images are usually limited because it is relative difficult to collect enough palmprint samples, making most existing deep-learning-based methods ineffective. In this paper, we propose a heuristic palmprint recognition method by extracting triple types of palmprint features without requiring any training samples. We first extract the most important inherent features of a palmprint, including the texture, gradient and direction features, and encode them into triple-type feature codes. Then, we use the block-wise histograms of the triple-type feature codes to form the triple feature descriptors for palmprint representation. Finally, we employ a weighted matching-score level fusion to calculate the similarity between two compared palmprint images of triple-type feature descriptors for palmprint recognition. Extensive experimental results on the three widely used palmprint databases clearly show the promising effectiveness of the proposed method.

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A type of coronavirus disease called COVID-19 is spreading all over the globe. Researchers and scientists are endeavoring to find new and effective methods to diagnose and treat this disease. This article presents an automated and fast system that identifies COVID-19 from X-ray radiographs of the chest using image processing and machine learning algorithms. Initially, the system extracts the feature descriptors from the radiographs of both healthy and COVID-19 affected patients using the speeded up robust features algorithm. Then, visual vocabulary is built by reducing the number of feature descriptors via quantization of feature space using the K-means clustering algorithm. The visual vocabulary train the support vector machine (SVM) classifier. During testing, an X-ray radiograph's visual vocabulary is sent to the trained SVM classifier to detect the absence or presence of COVID-19. The study used the dataset of 340 X-ray radiographs, 170 images of each Healthy and Positive COVID-19 class. During simulations, the dataset split into training and testing parts at various ratios. After training, the system does not require any human intervention and can process thousands of images with high precision in a few minutes. The performance of the system is measured using standard parameters of accuracy and confusion matrix. We compared the performance of the proposed SVM-based classier with the deep-learning-based convolutional neural networks (CNN). The SVM yields better results than CNN and achieves a maximum accuracy of up to 94.12%.

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In this paper, we evaluate the effect of scale analysis as well as the filtering process on the performances of an original compressed-domain classifier in the field of material surface topographies classification. Each surface profile is multiscale analyzed by using a Gaussian Filter analyzing method to be decomposed into three multiscale filtered image types: Low-pass (LP), Band-pass (BP), and High-pass (HP) filtered versions, respectively. The complete set of filtered image data constitutes the collected database. First, the images are lossless compressed using the state-of-the art High-efficiency video coding (HEVC) video coding standard. Then, the Intra-Prediction Modes Histogram (IPHM) feature descriptor is computed directly in the compressed domain from each HEVC compressed image. Finally, we apply the IPHM feature descriptors as an input of a Support Vector Machine (SVM) classifier. SVM is introduced here to strengthen the performances of the proposed classification system thanks to the powerful properties of machine learning tools. We evaluate the proposed solution we called "HEVC Multiscale Decomposition" (HEVC-MD) on a huge database of nearly 42,000 multiscale topographic images. A simple preliminary version of the algorithm reaches an accuracy of 52%. We increase this accuracy to 70% by using the multiscale analysis of the high-frequency range HP filtered image data sets. Finally, we verify that considering only the highest-scale analysis of low-frequency range LP was more appropriate for classifying our six surface topographies with an accuracy of up to 81%. To compare these new topographical descriptors to those conventionally used, SVM is applied on a set of 34 roughness parameters defined on the International Standard GPS ISO 25178 (Geometrical Product Specification), and one obtains accuracies of 38%, 52%, 65%, and 57% respectively for Sa, multiscale Sa, 34 roughness parameters, and multiscale ones. Compared to conventional roughness descriptors, the HEVC-MD descriptors increase surfaces discrimination from 65% to 81%.

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ECG beat type analysis is important in the detection of various heart diseases. The ECG beats give useful information about the status of the monitored heart condition. Up to now, various artificial intelligence-based methods have been proposed for ECG based heart failure detection. These methods were generally based on either time or frequency domain signal processing routines. In this study, we propose a different approach for ECG beat classification. The proposed approach is based on image processing. Thus, the initial step of the proposed work is converting the ECG beat signals to the ECG beat images. To do that, the ECG beat snapshots are initially saved as ECG beat images and then local feature descriptors are considered for feature extraction from ECG beat images. Eight local feature descriptors namely Local Binary Patterns, Frequency Decoded LBP, Quaternionic Local Ranking Binary Pattern, Binary Gabor Pattern, Local Phase Quantization, Binarized Statistical Image Features, CENsus TRansform hISTogram and Pyramid Histogram of Oriented Gradients are considered for feature extraction. The Support Vector Machines (SVM) classifier is used in the classification stage of the study. Linear, Quadratic, Cubic and Gaussian kernel functions are used in the SVM classifier. Five types of ECG beats from the MIT-BIH arrhythmia dataset are considered in experiments and the classification accuracy is used for performance measure. To construct a balanced training and test sets, 5000 and 10,000 ECG beat samples are randomly selected and are used in experiments in tenfold cross-validation fashion. The obtained results show that the proposed method is quite efficient where the calculated accuracy score is 99.9% and the comparisons with the state-of-the-art method show that the proposed method outperforms other methods.

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Quorum-sensing peptides (QSPs) are the signal molecules that are closely associated with diverse cellular processes, such as cell-cell communication, and gene expression regulation in Gram-positive bacteria. It is therefore of great importance to identify QSPs for better understanding and in-depth revealing of their functional mechanisms in physiological processes. Machine learning algorithms have been developed for this purpose, showing the great potential for the reliable prediction of QSPs. In this study, several sequence-based feature descriptors for peptide representation and machine learning algorithms are comprehensively reviewed, evaluated and compared. To effectively use existing feature descriptors, we used a feature representation learning strategy that automatically learns the most discriminative features from existing feature descriptors in a supervised way. Our results demonstrate that this strategy is capable of effectively capturing the sequence determinants to represent the characteristics of QSPs, thereby contributing to the improved predictive performance. Furthermore, wrapping this feature representation learning strategy, we developed a powerful predictor named QSPred-FL for the detection of QSPs in large-scale proteomic data. Benchmarking results with 10-fold cross validation showed that QSPred-FL is able to achieve better performance as compared to the state-of-the-art predictors. In addition, we have established a user-friendly webserver that implements QSPred-FL, which is currently available at http://server.malab.cn/QSPred-FL. We expect that this tool will be useful for the high-throughput prediction of QSPs and the discovery of important functional mechanisms of QSPs.

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Many biological objects are barely distinguished with the brightfield microscope because they appear transparent, translucent and colourless. One simple way to make such specimens visible without compromising contrast and resolution is by controlling the amount and the directionality of the illumination light. Oblique illumination is an old technique described by many scientists and microscopists that however has been largely neglected in favour of other alternative methods. Oblique lighting (OL) is created by illuminating the sample by only a portion of the light coming from the condenser. If properly used it can improve the resolution and contrast of transparent specimens such as diatoms. In this paper a quantitative evaluation of OL in brigthfield microscopy is presented. Several feature descriptors were selected for characterising contrast and sharpness showing that in general OL provides better performance for distinguishing minute details compared to other lighting modalities. Oblique lighting is capable to produce directionally shadowed differential contrast images allowing to observe phase details in a similar way to differential contrast images (DIC) but at lower cost. The main advantage of OL is that the resolution of the light microscope can be increased by effectively doubling the angular aperture. OL appears as a cost-effective technique both for the amateur and professional scientist that can be used as a replacement of DIC or phase contrast when resources are scarce.

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Scanning electron microscopy (SEM) imaging has been a principal component of many studies in biomedical, mechanical, and materials sciences since its emergence. Despite the high resolution of captured images, they remain two-dimensional (2D). In this work, a novel framework using sparse-dense correspondence is introduced and investigated for 3D reconstruction of stereo SEM images. SEM micrographs from microscopic samples are captured by tilting the specimen stage by a known angle. The pair of SEM micrographs is then rectified using sparse scale invariant feature transform (SIFT) features/descriptors and a contrario RANSAC for matching outlier removal to ensure a gross horizontal displacement between corresponding points. This is followed by dense correspondence estimation using dense SIFT descriptors and employing a factor graph representation of the energy minimization functional and loopy belief propagation (LBP) as means of optimization. Given the pixel-by-pixel correspondence and the tilt angle of the specimen stage during the acquisition of micrographs, depth can be recovered. Extensive tests reveal the strength of the proposed method for high-quality reconstruction of microscopic samples.