RESUMO
BACKGROUND: In the Yucatán Peninsula, Mexico, Triatoma dimidiata is the main vector of Trypanosoma cruzi, the causative agent of Chagas disease. Little effort has been made to identify blood meal sources of T. dimidiata in natural conditions in this region, although this provides key information to disentangle T. cruzi transmission cycles and dynamics and guide the development of more effective control strategies. We identified the blood meals of a large sample of T. dimidiata bugs collected in different ecotopes simultaneously with the assessment of bug infection with T. cruzi, to disentangle the dynamics of T. cruzi transmission in the region. METHODS: A sample of 248 T. dimidiata bugs collected in three rural villages and in the sylvatic habitat surrounding these villages was used. DNA from each bug midgut was extracted and bug infection with T. cruzi was assessed by PCR. For blood meal identification, we used a molecular assay based on cloning and sequencing following PCR amplification with vertebrate universal primers, and allowing the detection of multiple blood meals in a single bug. RESULTS: Overall, 28.7% of the bugs were infected with T. cruzi, with no statistical difference between bugs from the villages or from sylvatic ecotopes. Sixteen vertebrate species including domestic, synanthropic and sylvatic animals, were identified as blood meal sources for T. dimidiata. Human, dog and cow were the three main species identified, in bugs collected in the villages as well as in sylvatic ecotopes. Importantly, dog was highlighted as the main blood meal source after human. Dog was also the most frequently identified animal together with human within single bugs, and tended to be associated with the infection of the bugs. CONCLUSIONS: Dog, human and cow were identified as the main mammals involved in the connection of sylvatic and domestic transmission cycles in the Yucatán Peninsula, Mexico. Dog appeared as the most important animal in the transmission pathway of T. cruzi to humans, but other domestic and synanthropic animals, which most were previously reported as important hosts of T. cruzi in the region, were evidenced and should be taken into account as part of integrated control strategies aimed at disrupting parasite transmission.
Assuntos
Sangue , Doença de Chagas/transmissão , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificação , Animais , Bovinos , Cães , Feminino , Humanos , Insetos Vetores/parasitologia , Insetos Vetores/fisiologia , Masculino , México , Triatoma/fisiologiaRESUMO
Non-domiciliated intrusive triatomine vectors are responsible for a low but significant transmission of Trypanosoma cruzi to humans. Their control is a challenge as insecticide spraying is of limited usefulness, and alternative strategies need to be developed for a sustainable control. We performed a non-randomized controlled trial of an Ecohealth intervention based on window insect screens and community participation to reduce house infestation by Triatoma dimidiata in two rural villages in Yucatan, Mexico. Efficacy of the intervention was measured over a three years follow-up period and entomological indicators showed that the proportion of triatomines found inside houses was significantly reduced in houses with insect screens, which effectively kept more bugs on the outside of houses. Using a previously developed model linking entomological data to the prevalence of infection in human, we predicted that the intervention would lead to a 32% reduction in yearly incidence and in the prevalence of T. cruzi infection. The cost for the coverage of all the windows of a house was of comparable magnitude to what families currently spend on various domestic insecticide, and most screens were still in good conditions after three years. In conclusion, the Ecohealth approach proposed here is effective for the long-term and sustainable control of intrusive T. dimidiata vectors in the Yucatan peninsula, Mexico. This strategy may also be easily adapted to other intrusive triatomine species as well as other regions/countries with comparable eco-epidemiological settings, and would be an excellent component of a larger integrated program for the control of a variety of other vector-borne diseases, bringing additional benefits to the communities. Our results should encourage a further scaling-up of our implementation strategy in additional villages in the region.
Assuntos
Doença de Chagas/prevenção & controle , Controle de Insetos/métodos , Triatoma/fisiologia , Trypanosoma cruzi/fisiologia , Animais , Doença de Chagas/parasitologia , Doença de Chagas/transmissão , Habitação , Humanos , Insetos Vetores/efeitos dos fármacos , Insetos Vetores/parasitologia , Insetos Vetores/fisiologia , Inseticidas/farmacologia , México , Saúde da População Rural , Triatoma/efeitos dos fármacos , Triatoma/parasitologia , Trypanosoma cruzi/efeitos dos fármacos , Trypanosoma cruzi/parasitologiaRESUMO
Compared with South America, there is a lack of epidemiologic studies about the risk of congenital transmission of Trypanosoma cruzi in Central America and Mexico. It has been suggested that T. cruzi genotypes might differ by region and that congenital transmission might vary according to the parasite's genotype. Our objective was to compare T. cruzi congenital transmission rates in three countries. We performed an observational prospective study in 2011-2014 enrolling women at delivery in one hospital in Argentina, two hospitals in Honduras, and two hospitals in Mexico. Congenital T. cruzi infection was defined as the presence of one or more of the following criteria: presence of parasites in cord blood (direct parasitological microscopic examination) with positive polymerase chain reaction (PCR) in cord blood, presence of parasites in infant's blood at 4-8 weeks (direct parasitological microscopic examination), and persistence of T. cruzi-specific antibodies at 10 months, as measured by at least two tests. Among 28,145 enrolled women, 347 had at least one antibody rapid test positive in cord blood and a positive enzyme-linked immunosorbent assay in maternal blood. PCR in maternal blood was positive in 73.2% of the cases, and genotyping identified a majority of non-TcI in the three countries. We found no (0.0%; 95% confidence interval [CI]: 0.0, 2.0) confirmed congenital case in Honduras. Congenital transmission was 6.6% (95% CI: 3.1, 12.2) in Argentina and 6.3% (95% CI: 0.8, 20.8) in Mexico. Trypanosoma cruzi non-TcI predominated and risks of congenital transmission were similar in Argentina and Mexico.
Assuntos
Doença de Chagas/transmissão , Transmissão de Doença Infecciosa/estatística & dados numéricos , Adulto , Doença de Chagas/epidemiologia , Feminino , Sangue Fetal/parasitologia , Honduras/epidemiologia , Humanos , Recém-Nascido , México/epidemiologia , Gravidez , Estudos Prospectivos , Estatísticas não Paramétricas , Trypanosoma cruzi/patogenicidadeRESUMO
BACKGROUND: Chagas disease, caused by the parasite Trypanosoma cruzi, is mainly transmitted by blood-sucking bugs called triatomines. In the Yucatán Peninsula, Mexico, the main vector of T. cruzi is Triatoma dimidiata. While this species may colonize houses in other regions, it is mostly intrusive in Yucatán: it generally lives in sylvan and peridomestic areas, and frequently enters inside homes, likely attracted by potential vertebrate hosts, without establishing colonies. Bugs collected inside homes have a low nutritional status, suggesting that they cannot efficiently feed inside these houses. We hypothesized that this low nutritional status and limited colonization may be associated, at least in part, with the local practice in Mayan communities to sleep in hammocks instead of beds, as this sleeping habit could be an obstacle for triatomines to easily reach human hosts, particularly for nymphal instars which are unable to fly. METHODS: We used an experimental chamber in which we placed a miniature bed in one side and a miniature hammock on the other side. After placing a mouse enclosed in a small cage on the bed and another one in the hammock as baits, T. dimidiata bugs were released in the chamber and their activity was video recorded during the night. RESULTS: T. dimidiata adults and nymphs were able to reach the mouse in bed significantly more often than the mouse in hammock (Binomial test, P < 0.0001). Moreover, females reached the mice twice as often as did males. Most of the adult bugs reached the mouse in bed by walking, while they reached the mouse in hammock by flying. Nymphs presented a host-seeking index ten times lower than adult bugs and were also able, on a few occasions (4/132 released bugs), to reach the mouse in hammock. CONCLUSIONS: We conclude that sleeping in hammocks, as done in rural Yucatán, makes human hosts less accessible to the bugs. This, combined with other factors (e.g. absence of domestic animals sleeping inside houses), may explain, at least in part, the low nutritional status of bugs collected inside homes and the limited colonization of houses by T. dimidiata in the region. Nevertheless, while this sleeping habit limits contact with the bugs, it does not confer complete protection as adult bugs as well as some nymphs were still able to reach the host in hammock in our study.
Assuntos
Comportamento Alimentar , Comportamento de Busca por Hospedeiro , Insetos Vetores , Sono , Triatoma/fisiologia , Animais , México , CamundongosRESUMO
Chagas disease is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. In Ecuador, Triatoma dimidiata and Rhodnius ecuadoriensis are the main vector species, responsible for over half of the cases of T. cruzi infection in the country. T. dimidiata is believed to have been introduced in Ecuador during colonial times, and its elimination from the country is thus believed to be feasible. We investigated here the molecular ecology of T. dimidiata and T. cruzi in costal Ecuador to further guide control efforts. Analysis of the Internal Transcribed Spacer 2 (ITS-2) of 23 specimens from Progreso, Guayas, unambiguously supported the likely importation of T. dimidiata from Central America to Ecuador. The observation of a very high parasite infection rate (54%) and frequent feeding on humans (3/5) confirmed a continued risk of transmission to humans. All genotyped parasites corresponded to TcI DTU and Trypanosoma rangeli was not detected in T. dimidiata. TcI subgroups corresponded to TcIa (25%), and mixed infections with TcIa and TcId (75%). Further studies should help clarify T. cruzi genetic structure in the country, and the possible impact of the introduction of T. dimidiata on the circulating parasite strains. The elevated risk posed by this species warrants continuing efforts for its control, but its apparent mobility between peridomestic and domestic habitats may favor reinfestation following insecticide spraying.
Assuntos
Doença de Chagas/transmissão , DNA Intergênico/genética , Espécies Introduzidas , Estágios do Ciclo de Vida/genética , Filogenia , Triatoma/genética , Animais , Doença de Chagas/epidemiologia , Doença de Chagas/parasitologia , Ecossistema , Equador/epidemiologia , Comportamento Alimentar/fisiologia , Feminino , Genótipo , Humanos , Controle de Insetos , Insetos Vetores , Masculino , Epidemiologia Molecular , Rhodnius/parasitologia , Triatoma/classificação , Triatoma/crescimento & desenvolvimento , Triatoma/parasitologia , Trypanosoma cruzi/patogenicidade , Trypanosoma cruzi/fisiologiaRESUMO
BACKGROUND: Publishing negative seroprevalence studies not only helps to have more accurate seroprevalence estimates but also allows calculating the specificity of the diagnostic tests used. We performed a population-based Trypanosoma cruzi seroprevalence survey in a community in central Mexico. RESULTS: We surveyed 204 women and children and collected blood by finger prick. We performed rapid tests (Stat-Pak, Chembio, Inc., Medford, New York) and recombinant Chagas ELISA tests v3.0 (Wiener, Rosario, Argentina). All rapid tests and all ELISA tests were negative. CONCLUSION: The rapid test had 100 % of specificity compared to the ELISA.
Assuntos
Testes Diagnósticos de Rotina/normas , Ensaio de Imunoadsorção Enzimática/normas , Trypanosoma cruzi/imunologia , Adolescente , Adulto , Anticorpos Antiprotozoários/sangue , Doença de Chagas/diagnóstico , Doença de Chagas/epidemiologia , Doença de Chagas/imunologia , Doença de Chagas/parasitologia , Criança , Pré-Escolar , Feminino , Humanos , México/epidemiologia , Pessoa de Meia-Idade , Gravidez , Sensibilidade e Especificidade , Estudos SoroepidemiológicosRESUMO
The Tc24 calcium binding protein from the flagellar pocket of Trypanosoma cruzi is under evaluation as a candidate vaccine antigen against Chagas disease. Previously, a DNA vaccine encoding Tc24 was shown to be an effective vaccine (both as a preventive and therapeutic intervention) in mice and dogs, as evidenced by reductions in T. cruzi parasitemia and cardiac amastigotes, as well as reduced cardiac inflammation and increased host survival. Here we developed a suitable platform for the large scale production of recombinant Tc24 (rTc24) and show that when rTc24 is combined with a monophosphoryl-lipid A (MPLA) adjuvant, the formulated vaccine induces a Th1-biased immune response in mice, comprised of elevated IgG2a antibody levels and interferon-gamma levels from splenocytes, compared to controls. These immune responses also resulted in statistically significant decreased T. cruzi parasitemia and cardiac amastigotes, as well as increased survival following T. cruzi challenge infections, compared to controls. Partial protective efficacy was shown regardless of whether the antigen was expressed in Escherichia coli or in yeast (Pichia pastoris). While mouse vaccinations will require further modifications in order to optimize protective efficacy, such studies provide a basis for further evaluations of vaccines comprised of rTc24, together with alternative adjuvants and additional recombinant antigens.
Assuntos
Antígenos de Protozoários/imunologia , Doença de Chagas/prevenção & controle , Vacinas Protozoárias/imunologia , Trypanosoma cruzi/imunologia , Adjuvantes Imunológicos/administração & dosagem , Animais , Anticorpos Antiprotozoários/sangue , Antígenos de Protozoários/genética , Doença de Chagas/imunologia , Clonagem Molecular , Modelos Animais de Doenças , Escherichia coli/genética , Feminino , Expressão Gênica , Interferon gama/metabolismo , Leucócitos Mononucleares/imunologia , Lipídeo A/administração & dosagem , Camundongos Endogâmicos BALB C , Carga Parasitária , Parasitemia/prevenção & controle , Pichia/genética , Vacinas Protozoárias/administração & dosagem , Vacinas Protozoárias/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Baço/imunologia , Análise de Sobrevida , Células Th1/imunologia , Trypanosoma cruzi/genética , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologiaRESUMO
BACKGROUND: Non-domiciliated (intrusive) triatomine vectors remain a challenge for the sustainability of Chagas disease vector control as these triatomines are able to transiently (re-)infest houses. One of the best-characterized examples is Triatoma dimidiata from the Yucatan peninsula, Mexico, where adult insects seasonally infest houses between March and July. METHODS: We focused our study on three rural villages in the state of Yucatan, Mexico, in which we performed a situation analysis as a first step before the implementation of an ecohealth (ecosystem approach to health) vector control intervention. RESULTS: The identification of the key determinants affecting the transient invasion of human dwellings by T. dimidiata was performed by exploring associations between bug presence and qualitative and quantitative variables describing the ecological, biological and social context of the communities. We then used a participatory action research approach for implementation and evaluation of a control strategy based on window insect screens to reduce house infestation by T. dimidiata. CONCLUSIONS: This ecohealth approach may represent a valuable alternative to vertically-organized insecticide spraying. Further evaluation may confirm that it is sustainable and provides effective control (in the sense of limiting infestation of human dwellings and vector/human contacts) of intrusive triatomines in the region.
Assuntos
Doença de Chagas/prevenção & controle , Habitação/normas , Controle de Insetos/organização & administração , Triatoma/crescimento & desenvolvimento , Trypanosoma cruzi/patogenicidade , Animais , Doença de Chagas/transmissão , Reservatórios de Doenças , Ecossistema , Interações Hospedeiro-Parasita , Humanos , Insetos Vetores , México/epidemiologia , Inovação Organizacional , Vigilância da População , Características de Residência , População Rural , Estações do Ano , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificaçãoRESUMO
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and activation of CD8(+) T cells is crucial for a protective immune response. Therefore, the identification of antigens with major histocompatibility complex class I epitopes is a crucial step for vaccine development against T. cruzi. Our aim was to identify novel antigens and epitopes by immunoinformatics analysis of the parasite proteome (12 969 proteins) and to validate their immunotherapeutic potential in infected mice. We identified 172 predicted epitopes, using NetMHC and RANKPEP. The corresponding protein sequences were reanalyzed to generate a consensus prediction, and 26 epitopes were selected for in vivo validation. The interferon γ (IFN-γ) recall response of splenocytes from T. cruzi-infected mice confirmed that 10 of 26 epitopes (38%) induced IFN-γ production. The immunotherapeutic potential of a mixture of all 10 peptides was evaluated in infected mice. The therapeutic vaccine was able to control T. cruzi infection, as evidenced by reduced parasitemia, cardiac tissue inflammation, and parasite burden and increased survival. These findings illustrate the benefits of this approach for the rapid development of a vaccine against pathogens with large genomes. The identified peptides and the proteins from which they are derived are excellent candidates for the development of a vaccine against T. cruzi.
Assuntos
Doença de Chagas/prevenção & controle , Doença de Chagas/terapia , Biologia Computacional , Vacinas Protozoárias/imunologia , Vacinas Protozoárias/isolamento & purificação , Trypanosoma cruzi/genética , Trypanosoma cruzi/imunologia , Animais , Antígenos de Protozoários/genética , Antígenos de Protozoários/imunologia , Modelos Animais de Doenças , Epitopos de Linfócito T/genética , Epitopos de Linfócito T/imunologia , Interferon gama/metabolismo , Leucócitos Mononucleares/imunologia , Camundongos Endogâmicos BALB C , Miocárdio/patologia , Carga Parasitária , Parasitemia/prevenção & controle , Parasitemia/terapia , Baço/imunologia , Análise de SobrevidaRESUMO
BACKGROUND: Cutaneous leishmaniasis is a tropical disease affecting over one million patients annually and Leishmania (L.) mexicana is one of the major etiological agents in the Americas. Here we established the first experimental infection of L. (L.) mexicana in canids. METHODS: Beagle dogs were infected intradermally with culture-derived L. (L.) mexicana. We followed skin ulcer development, histopathological signs, parasite burden and the immune status of the infected dogs. RESULTS: All infected dogs developed uniform oval-craterform ulcers similar to those observed in humans, associated with mixed T helper 1/T helper 2 immune responses. Parasites were detected in the healed lesions 15 weeks post-infection. Higher anti-Leishmania IgG levels correlated with larger lesions and high IgG1/IgG2 ratio was associated with some level of splenomegaly. CONCLUSIONS: The canine model described in this work will be of use for further understanding of L. (L.) mexicana immunopathogenensis, and for drug and vaccine development.
Assuntos
Modelos Animais de Doenças , Leishmania mexicana , Leishmaniose Cutânea/parasitologia , Animais , Cães , Leishmaniose Cutânea/patologiaRESUMO
BACKGROUND: Chagas disease is a vector-borne disease of major importance in the Americas. Disease prevention is mostly limited to vector control. Integrated interventions targeting ecological, biological and social determinants of vector-borne diseases are increasingly used for improved control. METHODOLOGY/PRINCIPAL FINDINGS: We investigated key factors associated with transient house infestation by T. dimidiata in rural villages in Yucatan, Mexico, using a mixed modeling approach based on initial null-hypothesis testing followed by multimodel inference and averaging on data from 308 houses from three villages. We found that the presence of dogs, chickens and potential refuges, such as rock piles, in the peridomicile as well as the proximity of houses to vegetation at the periphery of the village and to public light sources are major risk factors for infestation. These factors explain most of the intra-village variations in infestation. CONCLUSIONS/SIGNIFICANCE: These results underline a process of infestation distinct from that of domiciliated triatomines and may be used for risk stratification of houses for both vector surveillance and control. Combined integrated vector interventions, informed by an Ecohealth perspective, should aim at targeting several of these factors to effectively reduce infestation and provide sustainable vector control.
Assuntos
Características da Família , Insetos Vetores , Características de Residência , Triatoma/crescimento & desenvolvimento , Animais , Animais Domésticos , Ecossistema , Humanos , México , População RuralRESUMO
Triatoma dimidiata is one of the primary vectors of Chagas disease. We previously documented the spatio-temporal infestation of houses by this species in the Yucatan peninsula, Mexico, and found that non-domiciliated triatomines were specifically attracted to houses. However, the factors mediating this attraction remained unclear. Artificial light has been known for a long time to attract many insect species, and therefore may contribute to the spread of different vector-borne diseases. Also, based on the collection of different species of triatomines with light traps, several authors have suggested that light might attract triatomines to houses, but the role of artificial light in house infestation has never been clearly demonstrated and quantified. Here we performed a spatial analysis of house infestation pattern by T. dimidiata in relation to the distribution of artificial light sources in three different villages from the Yucatan peninsula, Mexico. In all three villages, infested houses were significantly closer to public street light sources than non-infested houses (18.0 ± 0.6 vs 22.6 ± 0.4 m), and street lights rather than domestic lights were associated with house infestation. Accordingly, houses closer to a public street lights were 1.64 times more likely to be infested than houses further away (OR, CI95% 1.23-2.18). Behavioral experiments using a dual-choice chamber further confirmed that adult male and females were attracted to white light during their nocturnal activity. Attraction was also dependent on light color and decreased with increasing wavelength. While public lighting is usually associated with increased development, these data clearly show that it also directly contributes to house infestation by non-domiciliated T. dimidiata.
Assuntos
Doença de Chagas/transmissão , Habitação , Insetos Vetores/efeitos da radiação , Luz , Triatoma/efeitos da radiação , Animais , Comportamento Animal/efeitos da radiação , Feminino , Insetos Vetores/fisiologia , Masculino , Triatoma/fisiologiaRESUMO
Triatoma dimidiata is one of the main vectors of Chagas disease, and it has been shown to be a species complex. In the Yucatán peninsula, Mexico, vector populations are non-domiciliated, and the transmission of Trypanosoma cruzi thus critically relies on vector dispersal. This leads us to study the morphologic variations in T. dimidiata wings with respect to genetic factors (sex and genotype at the ITS-2 locus), geographic location, and T. cruzi-infection status. Females were found to have larger and more symmetrical wings than males. Wing shape was influenced by ITS-2 genotypes, although differences are unlikely sufficient to allow taxonomic discrimination of the sibling species. Hybrids were shown to have similar fluctuating asymmetries in wing size and shape as parental species, but the level of asymmetry in shape varied slightly between villages. The two later findings are consistent with a high level of gene flow between parental species, and the high dispersal potential of these non-domiciliated vectors. More surprisingly, individuals infected with T. cruzi were found to have larger wings than non-infected ones. This effect, which was consistently observed across sexes, genotypes and villages, is likely to be due to a direct impact of T. cruzi on insect development. Sex and infection status are thus likely to be key factors influencing vector dispersal with important impacts on disease transmission, since dispersal directly controls the domestic abundance of these vectors. These aspects should be investigated further to fully capture the ecology and evolution of Chagas disease transmission by non-domiciliated vectors.
Assuntos
Triatoma/anatomia & histologia , Triatoma/genética , Asas de Animais/anatomia & histologia , Animais , DNA Espaçador Ribossômico/genética , Feminino , Estudos de Associação Genética , Variação Genética , Genótipo , Masculino , México , Triatoma/parasitologia , Trypanosoma cruzi/parasitologiaRESUMO
Chagas disease is a major vector-borne disease, and regional initiatives based on insecticide spraying have successfully controlled domiciliated vectors in many regions. Non-domiciliated vectors remain responsible for a significant transmission risk, and their control is a challenge. We performed a proof-of-concept field trial to test alternative strategies in rural Yucatan, Mexico. Follow-up of house infestation for two seasons following the interventions confirmed that insecticide spraying should be performed annually for the effective control of Triatoma dimidiata; however, it also confirmed that insect screens or long-lasting impregnated curtains may represent good alternative strategies for the sustained control of these vectors. Ecosystemic peridomicile management would be an excellent complementary strategy to improve the cost-effectiveness of interventions. Because these strategies would also be effective against other vector-borne diseases, such as malaria or dengue, they could be integrated within a multi-disease control program.
Assuntos
Doença de Chagas/transmissão , Insetos Vetores , Triatoma/parasitologia , Animais , Doença de Chagas/epidemiologia , Doença de Chagas/prevenção & controle , Análise Custo-Benefício , Habitação , Inseticidas , México/epidemiologia , Vigilância da PopulaçãoRESUMO
Dirofilariasis (Dirofilaria immitis) and American trypanosomiasis (Trypanosoma cruzi) are zoonotic parasitic diseases affecting the hearts of a variety of mammalian host species, including dogs. In this study, some of the immunopathological characteristics of natural co-infection by these two parasites were compared with T. cruzi infection in dogs from Mexico. Antibody analysis in serum indicated significantly lower anti-T. cruzi IgG levels in co-infected dogs (n = 4) compared to those with T. cruzi infection alone (n = 9), together with a somewhat lower IgG2/IgG1 ratio. Cardiac tissue inflammation was limited and focal in co-infected animals whereas T. cruzi infected dogs had extensive and diffuse tissue inflammation. Three out of nine T. cruzi infected dogs and 1/4 of T. cruzi and D. immitis co-infected dogs showed cardiac alterations. The results showed that co-infections may interfere with host responses, and their significant prevalence (4/13 T. cruzi infected dogs) suggests that they should be taken into account by researchers and clinicians.
Assuntos
Doença de Chagas/veterinária , Dirofilariose/complicações , Doenças do Cão/epidemiologia , Animais , Anticorpos Anti-Helmínticos/sangue , Anticorpos Antiprotozoários/sangue , Doença de Chagas/complicações , Doença de Chagas/epidemiologia , Doença de Chagas/patologia , Dirofilaria immitis/imunologia , Dirofilariose/epidemiologia , Dirofilariose/patologia , Doenças do Cão/parasitologia , Doenças do Cão/patologia , Cães , Feminino , Masculino , Trypanosoma cruzi/imunologiaRESUMO
OBJECTIVE: Chagas disease is a major vector-borne parasitic disease in Latin America, primarily transmitted to humans by triatomine vectors. Non-domiciliated triatomine species such as Triatoma dimidiata in the Yucatan peninsula, Mexico, can transiently invade houses and are emerging as a major challenge to control Trypanosoma cruzi transmission to humans. We analyzed the spatio-temporal spreading of house infestation by T. dimidiata in four rural villages. METHODS: Triatomines were collected in four rural villages over a 2 years period, and the spatio-temporal patterns of infestation were analyzed. RESULTS: Triatomines were consistently more abundant at the periphery of villages than in centers, indicating a much higher risk of T. cruzi transmission at the periphery. Male T. dimidiata were found further in the center of the village, while females remained closer to the periphery, suggesting differential dispersal capabilities between sexes, although the timing of dispersal appeared identical. Surprisingly, infected females were consistently collected in houses much further from the surrounding bushes than non-infected females, while the distribution of males was unaffected by their T. cruzi infection status, suggesting an increased dispersal capability in infected females. CONCLUSION: The spatial structure of infestation should be taken into account for the prioritization of vector control activities within villages, and spatially targeted interventions may be explored. A potential vector manipulation by T. cruzi, observed for the first time in triatomines, may favor parasite transmission to new hosts.
Assuntos
Doença de Chagas/veterinária , Habitação/estatística & dados numéricos , Insetos Vetores/crescimento & desenvolvimento , Triatoma/crescimento & desenvolvimento , Trypanosoma cruzi/fisiologia , Animais , Doença de Chagas/epidemiologia , Doença de Chagas/transmissão , Feminino , Sistemas de Informação Geográfica , Interações Hospedeiro-Parasita , Insetos Vetores/parasitologia , Masculino , México/epidemiologia , Dinâmica Populacional , Saúde da População Rural/estatística & dados numéricos , Estações do Ano , Fatores Sexuais , Conglomerados Espaço-Temporais , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificaçãoRESUMO
Triatoma dimidiata is one of the major Chagas disease vectors, with an extensive diversity in its morphology, habitat, and level of domiciliation. Molecular studies based on the internal transcribed spacer 2 (ITS-2) have subdivided this species into four potential taxonomic groups. Using both ITS-2 and cytochrome B markers, we confirmed the sibling species status of ITS-2 Group 3 and detected an apparent sympatry of ITS-2 Groups 2 and 3 in the Yucatan peninsula, Mexico. Here we examine the geographic distribution of T. dimidiata ITS-2 genotypes in the region and compare their egg production and Trypanosoma cruzi infection rates, as indicators of biological differences between groups. PCR genotyping of large natural populations showed an extensive sympatry of Groups 2 and 3 in most of the peninsula, often within the same house. We also detected a large proportion of individuals displaying ITS-2 sequences from both Groups 2 and 3, suggesting hybridization. Analysis of ITS-2 genotype frequencies indicated a strong departure from Hardy-Weinberg equilibrium in female hybrids, but not in males, due to a large heterozygote deficit. These results suggest random mating between ITS-2 Groups 2 and 3 combined with reduced viability and/or survival in female hybrids. This and other factors may allow for the maintenance of distinct ITS-2 Groups 2 and 3 populations despite high hybrid frequencies. Importantly, T. cruzi infection was much higher in hybrids compared to ITS-2 Groups 2 and 3 individuals, but all three genotypes appeared to seasonally infest houses in a similar manner in the region. These findings warrant further studies on T. dimidiata taxonomy and its epidemiologic implications.
Assuntos
Doença de Chagas/epidemiologia , Especificidade da Espécie , Triatoma/fisiologia , Animais , Doença de Chagas/transmissão , Citocromos b/análise , Citocromos b/genética , DNA Intergênico/análise , DNA Intergênico/genética , Feminino , Variação Genética , Genoma de Inseto , Geografia , Humanos , Hibridização Genética , Insetos Vetores/parasitologia , Insetos Vetores/fisiologia , Masculino , México/epidemiologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificaçãoRESUMO
Chagas disease is caused by Trypanosoma cruzi and dogs are an important reservoir of the parasite as well as a good model for the study of the pathogenesis of the disease. We aimed here at characterizing the immunopathology of naturally infected dogs in Merida, Yucatan, Mexico. Following an initial screening for T. cruzi seropositive stray dogs, we examined 9 seropositive and 10 seronegative animals. High lymphocytes and low monocytes counts were observed in peripheral blood from seropositives dogs. Three of nine seropositive dogs presented electrocardiographic alterations including right bundle branch block, sinusal block and QRS complex alterations and some right ventricle enlargement was noted. Histopathologic analysis of cardiac walls revealed significant inflammation with a clear tropism for the right ventricle, although most walls were affected. Seropositive dogs presented low IgG1 and high IgG2 levels. Higher IgG1 levels were associated with increased cardiac index and myocarditis, suggesting that a Th2 immune response leads to susceptibility and increased disease severity. These observations shed some light on the mechanisms of pathogenesis of Chagas disease in dogs, and provide a good framework for the evaluation of novel drugs and vaccines in this animal model.
Assuntos
Doença de Chagas/veterinária , Doenças do Cão/imunologia , Trypanosoma cruzi , Animais , Anticorpos Antiprotozoários/sangue , Doença de Chagas/imunologia , Doença de Chagas/patologia , DNA de Protozoário/análise , Doenças do Cão/patologia , Cães , Coração/parasitologia , Imunoglobulina G/sangueRESUMO
Leishmaniasis is a neglected disease with an estimated 12 million infected people. The recent completion of the sequencing of the Leishmania major genome has opened opportunities for the identification of targets for vaccine development. We present here the first attempt at identifying novel vaccine candidates by whole genome analysis. We predicted CD8(+) T cell epitopes from the L. major proteome and validated in vivo in mice the immunogenicity of some of the best predicted epitopes. Consensus epitope predictions from 8272 annotated protein sequences with 5-8 different algorithms allowed the identification of 78 class I CD8(+) epitopes. BALB/c mice were immunized with 26 synthetic peptides corresponding to the most likely epitopes. Fourteen (54%) resulted immunogenic, with eight being strong inducers of T cell IFNgamma production. None of the proteins from which the epitopes are derived are differentially expressed, only two may be surface proteins, eight have putative enzymatic, and metabolic activities. These epitopes and proteins represent new antigen candidates for further studies. While pathogen genomes have not yet delivered their full promise in terms of human health applications, our study opens the way for extensive genome mining for antigen identification and vaccine development against Leishmania and other pathogens.
Assuntos
Biologia Computacional/métodos , Epitopos de Linfócito T/imunologia , Leishmania major/imunologia , Proteoma/análise , Proteoma/imunologia , Vacinas/imunologia , Animais , Linfócitos T CD8-Positivos/imunologia , Epitopos de Linfócito T/análise , Epitopos de Linfócito T/genética , Genoma , Humanos , Leishmania major/genética , Camundongos , Camundongos Endogâmicos BALB C , Peptídeos/administração & dosagem , Peptídeos/química , Peptídeos/imunologia , VacinaçãoRESUMO
In the Yucatán Peninsula of Mexico, the main vector of Chagas disease is Triatoma dimidiata. Field studies suggest that natural transmission occurs through transient and seasonal invasion of houses by sylvatic/peridomestic triatomines, rather than through persistent domiciliated bug populations. We investigated the genetic structure of T. dimidiata populations, using morphometry and microsatellite markers, to assess dispersal of individuals in this triatomine species and to understand the dynamics of domestic infestation. We observed low phenotypic and genetic differentiation among populations from different villages, with an FST of only 0.0553, which suggested a weak but significant population structure at this level. Similarly low but significant differences were observed among populations from the same village but different biotopes (sylvatic, peridomestic, and domestic), with FST values ranging from 0.0096 to 0.0455. These data suggested elevated dispersal of bugs between biotopes (Nm = 5-25), which was confirmed by likelihood and Bayesian assignment tests. A proportion of bugs collected within domiciles were significantly assigned to peridomestic and sylvatic areas. This study showed that T. dimidiata has important dispersal capabilities that can explain the seasonal pattern of domicile infestation by peridomestic and sylvatic bugs. Therefore, dispersal should be taken into account in the design of effective vector control strategies.