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Disentangling Trypanosoma cruzi transmission cycle dynamics through the identification of blood meal sources of natural populations of Triatoma dimidiata in Yucatán, Mexico.
Moo-Millan, Joel Israel; Arnal, Audrey; Pérez-Carrillo, Silvia; Hernandez-Andrade, Anette; Ramírez-Sierra, María-Jesús; Rosado-Vallado, Miguel; Dumonteil, Eric; Waleckx, Etienne.
Afiliação
  • Moo-Millan JI; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Arnal A; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Pérez-Carrillo S; Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico City, México.
  • Hernandez-Andrade A; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Ramírez-Sierra MJ; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Rosado-Vallado M; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Dumonteil E; Laboratorio de Parasitología, Centro de Investigaciones Regionales "Dr Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
  • Waleckx E; Department of Tropical Medicine, Tulane University, School of Public Health and Tropical Medicine, New Orleans, LA, USA.
Parasit Vectors ; 12(1): 572, 2019 Nov 29.
Article em En | MEDLINE | ID: mdl-31783778
BACKGROUND: In the Yucatán Peninsula, Mexico, Triatoma dimidiata is the main vector of Trypanosoma cruzi, the causative agent of Chagas disease. Little effort has been made to identify blood meal sources of T. dimidiata in natural conditions in this region, although this provides key information to disentangle T. cruzi transmission cycles and dynamics and guide the development of more effective control strategies. We identified the blood meals of a large sample of T. dimidiata bugs collected in different ecotopes simultaneously with the assessment of bug infection with T. cruzi, to disentangle the dynamics of T. cruzi transmission in the region. METHODS: A sample of 248 T. dimidiata bugs collected in three rural villages and in the sylvatic habitat surrounding these villages was used. DNA from each bug midgut was extracted and bug infection with T. cruzi was assessed by PCR. For blood meal identification, we used a molecular assay based on cloning and sequencing following PCR amplification with vertebrate universal primers, and allowing the detection of multiple blood meals in a single bug. RESULTS: Overall, 28.7% of the bugs were infected with T. cruzi, with no statistical difference between bugs from the villages or from sylvatic ecotopes. Sixteen vertebrate species including domestic, synanthropic and sylvatic animals, were identified as blood meal sources for T. dimidiata. Human, dog and cow were the three main species identified, in bugs collected in the villages as well as in sylvatic ecotopes. Importantly, dog was highlighted as the main blood meal source after human. Dog was also the most frequently identified animal together with human within single bugs, and tended to be associated with the infection of the bugs. CONCLUSIONS: Dog, human and cow were identified as the main mammals involved in the connection of sylvatic and domestic transmission cycles in the Yucatán Peninsula, Mexico. Dog appeared as the most important animal in the transmission pathway of T. cruzi to humans, but other domestic and synanthropic animals, which most were previously reported as important hosts of T. cruzi in the region, were evidenced and should be taken into account as part of integrated control strategies aimed at disrupting parasite transmission.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triatoma / Trypanosoma cruzi / Sangue / Doença de Chagas Tipo de estudo: Diagnostic_studies Limite: Animals / Female / Humans / Male País/Região como assunto: Mexico Idioma: En Revista: Parasit Vectors Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triatoma / Trypanosoma cruzi / Sangue / Doença de Chagas Tipo de estudo: Diagnostic_studies Limite: Animals / Female / Humans / Male País/Região como assunto: Mexico Idioma: En Revista: Parasit Vectors Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido