RESUMO
PURPOSE: This study evaluated the immunohistochemical presence of Indian Hedgehog (IHH), transforming growth factor-ß (TGF-ß), and parathyroid-1 receptor (PTH1R) in calvaria bone repair, and compared these results with the histological bone matrix features in defects treated with autograft in the presence or absence of L-PRP. MATERIAL AND METHODS: An artificial bone defect measuring 5 × 1 mm was produced in the calvaria of 28 Wistar rats. Randomly the defects were treated with autograft and autograft mixed with L-PRP. The animals were euthanized at 15 and 40 days post-surgery. Data were analyzed by Student-Newman-Keuls test (p ≤ .05) for immunohistochemical interpretation. RESULTS: The results revealed that the histological characteristic of bone matrix deposited in the defect was different in the defects treated with L-PRP. The group that received only the autograft demonstrated larger haversian bone matrix deposited, whereas the group that received autograft mixed with L-PRP revealed trabecular bone deposition. These results coincided with significantly higher immunopositivity for IHH, TGF-ß1, and PTH1R in the L-PRP group. CONCLUSION: These results suggest that L-PRP altered the biological characteristic of the autograft, increasing the bone cells IHH+ but inducing a trabecular bone associated with intense quantities of TGF-ß and PTH1R.
Assuntos
Autoenxertos/transplante , Matriz Óssea/fisiologia , Proteínas Hedgehog/análise , Leucócitos/fisiologia , Osteogênese/fisiologia , Plasma Rico em Plaquetas/fisiologia , Receptor Tipo 1 de Hormônio Paratireóideo/análise , Crânio/cirurgia , Fator de Crescimento Transformador beta1/análise , Animais , Doenças Ósseas/cirurgia , Matriz Óssea/patologia , Osso Esponjoso/patologia , Osso Esponjoso/fisiologia , Ósteon/patologia , Ósteon/fisiologia , Processamento de Imagem Assistida por Computador/métodos , Imuno-Histoquímica , Masculino , Fotografação/métodos , Distribuição Aleatória , Ratos , Ratos Wistar , Crânio/patologia , Crânio/fisiologiaRESUMO
Introduction and Objective: Because L-PRP constitutes an important source of growth factor that is associated with osteogenesis and fibrogenesis, the aim of this study was to evaluate the effect of L-PRP on the presence of collagen III and MMP-2 and MMP-9, while comparing these results by means of a histomorphometric analysis of bone matrix and fibrous deposition on bone repair. Material and methods: Four bone defects of 8 × 2 mm were created on the calvaria of 21 rabbits. The surgical defects were treated with either particulate autograft, particulate autograft mixed with L-PRP, or L-PRP alone. Animals were euthanized at 2, 4, and 6 weeks postoperative. Histomorphometric and immunohistochemical analyses were performed to assess repair time, as well as the expression of collagen III and MMPs. Results: In contrast to the results of the L-PRP-free groups, the histomorphometric results of the L-PRP groups demonstrated intense fibrotic deposition along with hindered bone matrix deposition. These results coincided with the larger occurrence of diffuse collagen III deposition and the scarce presence of MMP- 2 and -9 spread among the fibrous tissue. Conclusion: Thus, the results suggest that L-PRP not only induces an intense fibrosis rich in collagen III, which is not degraded, but also suppresses MMP-2 and -9 expressions, mimicking a similar pathological event as that of a cleft-palate or cranial suture.