Platelet-leukocyte-rich plasma (L-PRP) prevents the collagen III degradation and impairs the bone matrix development in artificial defect of rabbit calvaria, associated with suppression of the immunohistochemical expression of MMP-2 and MMP-9
RSBO (Impr.)
; 11(4): 328-335, Oct.-Dec. 2014. ilus, tab
Article
em En
| LILACS
| ID: lil-778299
Biblioteca responsável:
BR97.1
ABSTRACT
Introduction and Objective:
Because L-PRP constitutes an important source of growth factor that is associated with osteogenesis and fibrogenesis, the aim of this study was to evaluate the effect of L-PRP on the presence of collagen III and MMP-2 and MMP-9, while comparing these results by means of a histomorphometric analysis of bone matrix and fibrous deposition on bone repair. Material andmethods:
Four bone defects of 8 × 2 mm were created on the calvaria of 21 rabbits. The surgical defects were treated with either particulate autograft, particulate autograft mixed with L-PRP, or L-PRP alone. Animals were euthanized at 2, 4, and 6 weeks postoperative. Histomorphometric and immunohistochemical analyses were performed to assess repair time, as well as the expression of collagen III and MMPs.Results:
In contrast to the results of the L-PRP-free groups, the histomorphometric results of the L-PRP groups demonstrated intense fibrotic deposition along with hindered bone matrix deposition. These results coincided with the larger occurrence of diffuse collagen III deposition and the scarce presence of MMP- 2 and -9 spread among the fibrous tissue.Conclusion:
Thus, the results suggest that L-PRP not only induces an intense fibrosis rich in collagen III, which is not degraded, but also suppresses MMP-2 and -9 expressions, mimicking a similar pathological event as that of a cleft-palate or cranial suture.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
RSBO (Impr.)
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil