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Leukocyte-platelet-rich plasma diminishes bone matrix deposition in rat calvaria treated with autograft due to simultaneous increase in immunohistochemical expression of Indian Hedgehog, transforming growth factor-ß, and parathyroid-1 receptor.
Cerci, Daniel Xavier; Portela, Giovanna Schirmer; Cunha, Emanuelle Juliana; Grossi, João Ricardo de Almeida; Zielak, João Cesar; Araújo, Melissa Rodrigues; Scariot, Rafaela; Deliberador, Tatiana Miranda; Giovanini, Allan Fernando.
Afiliação
  • Cerci DX; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Portela GS; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Cunha EJ; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Grossi JR; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Zielak JC; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Araújo MR; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Scariot R; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Deliberador TM; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil.
  • Giovanini AF; Department of Masters and Ph.D Program in Clinical Dentistry, Positivo University, Curitiba, Brazil. Electronic address: afgiovanini@gmail.com.
J Craniomaxillofac Surg ; 43(8): 1470-7, 2015 Oct.
Article em En | MEDLINE | ID: mdl-26220885
PURPOSE: This study evaluated the immunohistochemical presence of Indian Hedgehog (IHH), transforming growth factor-ß (TGF-ß), and parathyroid-1 receptor (PTH1R) in calvaria bone repair, and compared these results with the histological bone matrix features in defects treated with autograft in the presence or absence of L-PRP. MATERIAL AND METHODS: An artificial bone defect measuring 5 × 1 mm was produced in the calvaria of 28 Wistar rats. Randomly the defects were treated with autograft and autograft mixed with L-PRP. The animals were euthanized at 15 and 40 days post-surgery. Data were analyzed by Student-Newman-Keuls test (p ≤ .05) for immunohistochemical interpretation. RESULTS: The results revealed that the histological characteristic of bone matrix deposited in the defect was different in the defects treated with L-PRP. The group that received only the autograft demonstrated larger haversian bone matrix deposited, whereas the group that received autograft mixed with L-PRP revealed trabecular bone deposition. These results coincided with significantly higher immunopositivity for IHH, TGF-ß1, and PTH1R in the L-PRP group. CONCLUSION: These results suggest that L-PRP altered the biological characteristic of the autograft, increasing the bone cells IHH+ but inducing a trabecular bone associated with intense quantities of TGF-ß and PTH1R.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Crânio / Matriz Óssea / Receptor Tipo 1 de Hormônio Paratireóideo / Plasma Rico em Plaquetas / Fator de Crescimento Transformador beta1 / Proteínas Hedgehog / Autoenxertos / Leucócitos Limite: Animals Idioma: En Revista: J Craniomaxillofac Surg Assunto da revista: ODONTOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Crânio / Matriz Óssea / Receptor Tipo 1 de Hormônio Paratireóideo / Plasma Rico em Plaquetas / Fator de Crescimento Transformador beta1 / Proteínas Hedgehog / Autoenxertos / Leucócitos Limite: Animals Idioma: En Revista: J Craniomaxillofac Surg Assunto da revista: ODONTOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido