RESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.
Assuntos
Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Animais , Brasil , Técnicas de Cultura de Células , Diferenciação Celular , Proliferação de Células , Feminino , Citometria de Fluxo/métodos , Cabras , Masculino , Modelos Animais , Fator 3 de Transcrição de Octâmero/isolamento & purificação , Antígeno Nuclear de Célula em Proliferação/isolamento & purificação , Vimentina/isolamento & purificaçãoRESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied. .
Assuntos
Animais , Feminino , Masculino , Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Brasil , Técnicas de Cultura de Células , Diferenciação Celular , Proliferação de Células , Citometria de Fluxo/métodos , Cabras , Modelos Animais , /isolamento & purificação , Antígeno Nuclear de Célula em Proliferação/isolamento & purificação , Vimentina/isolamento & purificaçãoRESUMO
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.(AU)
Assuntos
Animais , Cabras , Medula Óssea , Células-Tronco/classificação , Pesquisa com Células-Tronco , Citometria de Fluxo/veterinária , Técnicas de Cultura de Células , Antígeno Nuclear de Célula em Proliferação/análiseRESUMO
A cutia encontra-se entre uma das espécies mais intensamente predada em toda a Amazônia e nas regiões semiáridas do Nordeste do Brasil. Considerando a tendência atual do manejo de animais silvestres em cativeiro, este estudo teve como objetivo determinar valores de referência cardíaca para cutias criadas em cativeiro, baseados em avaliações radiográficas e eletrocardiográficas (ECG). Foram selecionadas cutias adultas sem sinais clínicos de doença cardíaca (n=30). Os animais foram contidos fisicamente e, posteriormente, realizados o ECG e a avaliação radiográfica. Mensurações padronizadas foram tomadas para estabelecer a análise estatística dos dados. A avaliação radiográfica e a análise do complexo P-QRS-T demostraram valores compatíveis com relatos pregressos em animais de companhia, assim como aos dados limitados disponíveis para outras espécies silvestres e exóticos, a exceção da orientação cardíaca em relação ao esterno (AIC), que mostrou uma pequena angulação, diferente do descrito em mamíferos, e da onda T, que mostrou amplitude semelhante à onda R em todos os animais estudados. Os dados obtidos permitiram a aquisição dos primeiros valores de referência para mensurações cardíacas em cutias e traçados de ECG, contribuindo para a maior compreensão da morfologia e eletrofisiologia cardíaca, na identificação de miocardiopatia nesses animais