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1.
Gigascience ; 132024 01 02.
Artículo en Inglés | MEDLINE | ID: mdl-38837946

RESUMEN

BACKGROUND: Theobroma grandiflorum (Malvaceae), known as cupuassu, is a tree indigenous to the Amazon basin, valued for its large fruits and seed pulp, contributing notably to the Amazonian bioeconomy. The seed pulp is utilized in desserts and beverages, and its seed butter is used in cosmetics. Here, we present the sequenced telomere-to-telomere genome of cupuassu, disclosing its genomic structure, evolutionary features, and phylogenetic relationships within the Malvaceae family. FINDINGS: The cupuassu genome spans 423 Mb, encodes 31,381 genes distributed in 10 chromosomes, and exhibits approximately 65% gene synteny with the Theobroma cacao genome, reflecting a conserved evolutionary history, albeit punctuated with unique genomic variations. The main changes are pronounced by bursts of long-terminal repeat retrotransposons at postspecies divergence, retrocopied and singleton genes, and gene families displaying distinctive patterns of expansion and contraction. Furthermore, positively selected genes are evident, particularly among retained and dispersed tandem and proximal duplicated genes associated with general fruit and seed traits and defense mechanisms, supporting the hypothesis of potential episodes of subfunctionalization and neofunctionalization following duplication, as well as impact from distinct domestication process. These genomic variations may underpin the differences observed in fruit and seed morphology, ripening, and disease resistance between cupuassu and the other Malvaceae species. CONCLUSIONS: The cupuassu genome offers a foundational resource for both breeding improvement and conservation biology, yielding insights into the evolution and diversity within the genus Theobroma.


Asunto(s)
Evolución Molecular , Genoma de Planta , Filogenia , Cromosomas de las Plantas , Genómica/métodos , Malvaceae/genética
2.
Foods ; 13(7)2024 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-38611418

RESUMEN

Coffee fruit cascara, which is the skin and pulp of the coffee cherry, has been authorized as a novel food for commercialization in the European Union. The present research assessed the feasibility of using spray drying to produce a soluble powder called instant cascara (IC), employing sun-dried ripe coffee cherry pulp as a raw material. Although there were no significant differences (p > 0.05) in the overall antioxidant capacity between the freeze-dried and spray-dried samples, after an in vitro simulation of the digestion process, the spray-dried sample was significantly (p < 0.05) more antioxidant. Both samples reduced physiological intracellular ROS and significantly decreased (p < 0.05) the secretion of the pro-inflammatory factor NO. Alkaloids and phenolic compounds were detected in intestinal digests. In conclusion, spray drying is a good technique for producing IC as its use does not affect its properties and causes less environmental impact than freeze drying, as calculated by life cycle assessment. Sensory analysis did not show significant differences between the commercial beverage and the IC beverage in the adult population. IC at 10 mg/mL was significantly less accepted in adolescents than the commercial beverage. Future work will include the reformulation of the IC beverage at 10 mg/mL, which has antioxidant and anti-inflammatory potential, to increase its hedonic acceptance in all consumer segments.

3.
Int J Biol Macromol ; 254(Pt 3): 128029, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37952330

RESUMEN

Four main water-soluble wampee fruit pulp polysaccharides, named CSP-I, CSP-II, CSP-III and CSP-IV, were isolated from Clausena lansium (Lour.) Skeels Guifei, therein CSP-IV content was higher than the others. All components possess certain anticoagulant activity demonstrated by prolonged activated partial thromboplastin time, especially CSP-IV, which suggests that CSP-IV plays anticoagulant effect through disturbing intrinsic coagulation pathway. The wampee polysaccharide CSP-IV with Mw of 510.1 kDa was mainly composed of Gal, Ara and GalA. Backbone of CSP-IV contains Gal, Ara and GalA, two kinds of side chains contain one monosaccharide Gal or Ara, both branch on Gal residue of backbone. CSP-IV has no the conformation of triple helix demonstrated by Congo red test. These results showed that CSP-IV is an acidic polysaccharide with potential anticoagulant activity via targeting intrinsic coagulation pathway.


Asunto(s)
Clausena , Frutas , Frutas/química , Agua/análisis , Polisacáridos/química , Anticoagulantes/farmacología
4.
Int J Mol Sci ; 24(3)2023 Jan 24.
Artículo en Inglés | MEDLINE | ID: mdl-36768615

RESUMEN

Annonaceae is a large family composed of more than 119 genera and more than 2500 species that are distributed in both tropical and subtropical areas. The Annona genus is a member of Annonaceae family, which encompasses about 175 species, most of which are native to Brazil and tropical America. This plant is commonly found on tropical and subtropical continents. Annona atemoya is a commercially important hybrid of A. squamosa and A. cherimola. Phytochemical investigations of A. atemoya leaves, fruit, and seeds have been conducted in limited studies. The purpose of this study was to investigate the constituents of the leaves, fruit pulp, and seeds of A. atemoya because few studies have reported their constituents. Annonaceous acetogenins were identified in the leaves and pulp of A. atemoya for the first time. Twenty compounds were identified: sixteen were acetogenins and four were alkaloids. Additionally, two compounds were isolated, and their structures were confirmed by spectroscopic analysis and compared with the results of previous studies. The concentration of acetogenins in the pulp was very low compared with that in the leaves, whereas the seeds were found to contain the highest concentrations and greatest diversity of compounds.


Asunto(s)
Alcaloides , Annona , Acetogeninas/química , Annona/química , Alcaloides/análisis , Extractos Vegetales/química , Semillas/química
5.
Front Nutr ; 9: 914679, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35958258

RESUMEN

Dimocarpus longan Lour. (also called as longan) is a subtropical and tropical evergreen tree belonging to the Sapindaceae family and is widely distributed in China, Southeast Asia and South Asia. The pulp of longan fruit is a time-honored traditional medicinal and edible raw material in China and some Asian countries. With the advancement of food therapy in modern medicine, longan fruit pulp as an edible medicinal material is expected to usher in its rapid development as a functional nutrient. As one of the main constituents of longan fruit pulp, longan fruit pulp polysaccharides (LPs) play an indispensable role in longan fruit pulp-based functional utilization. This review aims to outline the extraction and purification methods, structural characteristics and biological activities (such as immunoregulatory, anti-tumor, prebiotic, anti-oxidant, anti-inflammatory and inhibition of AChE activity) of LPs. Besides, the structure-activity relationship, application prospect and patent application of LPs were analyzed and summarized. Through the systematic summary, this review attempts to provide a theoretical basis for further research of LPs, and promote the industrial development of this class of polysaccharides.

6.
Foods ; 11(2)2022 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-35053886

RESUMEN

Various dried (by-)products from the Tunisian O. ficus-indica were elucidated for their proximate composition, fatty acid (FA) composition, inorganic elements, sugars, and polyphenols. Nopal and prickly pear peel and seeds were abundant in fiber (respectively, 28.39, 12.54, and 16.28%). Seeds had also high protein (17.34%) and may be source of an edible oil, due to lipids (9.65%) poor in saturated FAs (14.12%) and rich in linoleic acid (61.11%). Nopal and peel showed the highest levels of Mg (493.57 and 345.19 mg/100 g), K (6949.57 and 1820.83 mg/100 g), Mn (59.73 and 46.86 mg/Kg) and Fe (23.15 and 15.23 mg/Kg), while the fruit pulp predominantly constituted of sugars, glucose and arabinose being predominant (42.57 and 13.56 g/100 g). Total polyphenols widely varied among the Opuntia products (108.36-4785.36 mg GAE/100 g), being mainly represented by hydroxycinnamic and hydroxybenzoic acids, and flavonoids as well. In particular, peel may be revalorized for these valuable bioactives, including 4-hydroxybenzoic acid (484.95 mg/100 g), cinnamic acid (318.95 mg/100 g), rutin (818.94 mg/100 g), quercetin (605.28 mg/100 g), and several isorhamnetin and kaempferol glycosides. Overall, the Tunisian prickly pear cactus could encourage a sustainable production, an effective waste management, and may provide several benefits for human health, in accordance with the model of the Mediterranean diet.

7.
J Sci Food Agric ; 102(5): 1968-1977, 2022 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-34514612

RESUMEN

BACKGROUND: Pomegranate is a rich source of polyphenols and has been used as a dietary supplement and pharmaceutical ingredient. This study aimed to investigate the pomegranate fruit pulp polyphenols (PFP) with regard to their anti-obesity activity and gut microbiota-modulating effect in mice. Thirty-six 4-week-old specific pathogen-free C57BL/6J mice (weight: 17.7-20.8 g) were randomly divided into three groups and fed with low-fat diet (10% fat energy), high-fat diet (HFD) (45% fat energy), or HFD supplemented with PFP by intragastric administration for 14 weeks. The obesity-related clinical indicators were investigated, and the composition of fecal microbiota was analyzed by 16S rRNA sequencing. RESULTS: Our results showed that PFP treatment reduced HFD-induced body weight gain by 35.23% (P < 0.05), steatosis scores by 50% (P < 0.05) and insulin resistance by 56.84% (P < 0.05), compared with the mice fed HFD alone. Moreover, compared with the mice in the HFD group, PFP supplement changed the composition of the gut microbiota, and enriched Akkermansia muciniphila, Parabacteroides distasonis, Bacteroides acidifaciens, Mucispirillum schaedleri and Lachnospiraceae bacterium 28-4, which were negatively correlated with physical biomarkers, including body weight, glucose, triglycerides and total cholesterol. CONCLUSION: PFP alleviated HFD-induced obesity, insulin resistance and hepatic steatosis in mice, and the changes in the gut microbiota might be one of the potential mechanisms through which PFP improved obesity and obesity-related disorders, eventually benefiting the recipient. © 2021 Society of Chemical Industry.


Asunto(s)
Microbioma Gastrointestinal , Granada (Fruta) , Animales , Dieta Alta en Grasa/efectos adversos , Frutas , Ratones , Ratones Endogámicos C57BL , Obesidad/tratamiento farmacológico , Obesidad/microbiología , Polifenoles/farmacología , Polifenoles/uso terapéutico , ARN Ribosómico 16S
8.
Food Chem ; 372: 131290, 2022 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-34818735

RESUMEN

This review aims to evaluate the production and processing chain of camu-camu (Myrciaria dubia), giving suggestions to maximize the valorization of raw materials, demonstrating new product possibilities from processing to distribution and highlighting the suggested contributions. It is clear that despite the camu-camu pulp has important properties, a large part of its raw material, considered waste (around 50%) and formed by important bioactive compounds, can give rise to new products, such as bioactive extracts to be used by pharmaceutical, chemical and food industries, ingredients for bakery products, dairy and several others sectors, which constitutes opportunities, in addition to contributing to the reduction of agro-industrial waste and the preservation of the environment.


Asunto(s)
Myrtaceae , Frutas
9.
Braz. J. Pharm. Sci. (Online) ; 58: e19373, 2022. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1384009

RESUMEN

Abstract The main purposes of the current study were to formulate o/w nanoemulsions as a carrier for Tamarindus indica (tamarind) fruit pulp extract and to study the antioxidant and antibacterial potentials of nanoemulsions containing tamarind extract, focusing on cosmetic/hygiene applications. The o/w nanoemulsions using a mixture of Tween 80 and Span 80 as an emulsifier (5%w/w) were prepared by a high pressure homogenization process. Two concentrations of sweet tamarind extract, 3.3 and 6.6%w/w, based on the bioactivity study, were incorporated into the blank nanoemulsions to produce loaded nanoemulsions, F1-3.3TE (3.3%) and F1- 6.6TE (6.6%). As compared with the unloaded nanoemulsion, both tamarind extract loaded nanoemulsions showed reduced pH and significantly increased viscosity. Overall, the loaded nanoemulsions had droplet sizes of approximately 130 nm, zeta potential around -38 mV and polydispersity index (PDI) values less than 0.2. The nanoemulsion F1-3.3TE had better stability (e.g. significantly greater % tartaric acid content and lesser PDI value) than the nanoemulsion F1-6.6TE did. The antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl assay revealed that the nanoemulsions F1-3.3TE and F1-6.6TE had scavenging activities of 81.66 ± 0.77% and 63.80 ± 0.79%, respectively. However, antioxidant activity of these two formulations decreased under stress conditions (heating-cooling cycles). Such incidence did not occur for their antibacterial properties investigated by agar well diffusion technique. The two formulations exhibited inhibition zones of approximately 24.0-27.7 mm against Staphylococcus aureus and Staphylococcus epidermidis, responsible for malodor of underarms. The results suggest the potential of using sweet tamarind pulp extract loaded nanoemulsions as hygiene products.


Asunto(s)
Tamarindus/efectos adversos , Frutas/clasificación , Antibacterianos/análisis , Antioxidantes/análisis , Staphylococcus aureus/clasificación , Staphylococcus epidermidis/clasificación , Potencial zeta , Calefacción/instrumentación , Concentración de Iones de Hidrógeno , Métodos
10.
Microorganisms ; 9(9)2021 Sep 17.
Artículo en Inglés | MEDLINE | ID: mdl-34576876

RESUMEN

Increasing insight into the impact of the gut microbiota on human health has sustained the development of novel prebiotic ingredients. This exploratory study evaluated the prebiotic potential of baobab fruit pulp powder, which consists of pectic polysaccharides with unique composition as compared to other dietary sources, given that it is rich in low methoxylated homogalacturonan (HG). After applying dialysis procedures to remove simple sugars from the product (simulating their absorption along the upper gastrointestinal tract), 48 h fecal batch incubations were performed. Baobab fruit pulp powder boosted colonic acidification across three simulated human adult donors due to the significant stimulation of health-related metabolites acetate (+18.4 mM at 48 h), propionate (+5.5 mM at 48 h), and to a lesser extent butyrate (0.9 mM at 48 h). Further, there was a trend of increased lactate levels (+2.7 mM at 6h) and reduced branched chain fatty acid (bCFA) levels (-0.4 mM at 48 h). While Bacteroidetes levels increased for all donors, donor-dependent increases in Bifidobacteria, Lactobacilli, and Firmicutes were observed, stressing the potential interindividual differences in microbial composition modulation upon Baobab fruit pulp powder treatment. Overall, Baobab fruit pulp powder fermentation displayed features of selective utilization by host microorganisms and, thus, has promising prebiotic potential (also in comparison with the 'gold standard' prebiotic inulin). Further research will be required to better characterize this prebiotic potential, accounting for the interindividual differences, while aiming to unravel the potential resulting health benefits.

11.
MethodsX ; 8: 101481, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34434879

RESUMEN

High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive. To simplify the conventional phenol-chloroform based RNA extraction method, we modified the protocol with less steps as well as the removal of the use of phenol. In this protocol, the extraction buffer is composed of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and Dithiothreitol (DTT). The method facilitates efficient removal of polysaccharides and phenolic compounds from both fruit pulp and fruit peel. Additionally, the protocol is phenol-free and less toxic than traditional phenol-containing method. High-quality RNA, with RNA Integrity Number value > 8, isolated by this method is applicable for RNA sequencing and qPCR. Only 3-4 working hours are required for one batch of RNA isolation.•Our method replaces the use of phenol-chloroform with chloroform, making the extraction less toxic.•The bilberry friut RNA is of high-quality and purity with less time input.

12.
Proc Biol Sci ; 288(1953): 20210817, 2021 06 30.
Artículo en Inglés | MEDLINE | ID: mdl-34157866

RESUMEN

The dispersal-syndrome hypothesis posits that fruit traits are a product of selection by frugivores. Although criticized as adaptationist, recent studies have suggested that traits such as fruit or seed size, colour and odour exhibit signatures that imply selection by animal mutualists. These traits imply nutritional rewards (e.g. lipid, carbohydrate), attracting frugivores; however, this remains incompletely resolved. Here, we investigated whether fruit nutrients (lipid, sugar, protein, vitamin C, water content) moderate the co-adaptation of key disperser-group mutualisms. Multivariate techniques revealed that fruit nutrients assembled non-randomly and grouped according to key dispersal modes. Bird-dispersed fruits were richer in lipids than mammal-dispersed fruits. Mixed-dispersed fruits had significantly higher vitamin C than did mammal- or bird-dispersed fruits separately. Sugar and water content were consistently high irrespective of dispersal modes, suggesting that these traits appeal to both avian and mammalian frugivores to match high-energy requirements. Similarly, protein content was low irrespective of dispersal modes, corroborating that birds and mammals avoid protein-rich fruits, which are often associated with toxic levels of nitrogenous secondary compounds. Our results provide substantial over-arching evidence that seed disperser assemblages co-exert fundamental selection pressures on fruit nutrient trait adaptation, with broad implications for structuring fruit-frugivore mutualism and maintaining fruit trait diversity.


Asunto(s)
Frutas , Dispersión de Semillas , Animales , Aves , Mamíferos , Nutrientes
13.
Food Chem ; 361: 130144, 2021 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-34082387

RESUMEN

Effect of high-intensity ultrasound (HIU) compared with thermal treatments on baobab fruit pulp (BFP) quality and bioactive properties were investigated. HIU treatments, particularly at intensities of 687.5 W/cm2 for 5 min, and 344 W/cm2 for 15 min significantly (p < 0.05) increased the cloudiness index, ascorbic acid (AA) retention, total phenolic and flavonoid contents, and antioxidant capacity besides a more potent α-amylase and α-glucosidase inhibition relative to thermally treated samples. Moreover, the physicochemical parameters, colour index, and browning index were maintained with HIU besides lower 5-hydroxymethylfurfural values than thermal processing. HPLC analysis revealed that the content of most phenolic compounds was the highest in HIU treatments besides a 235-256% increase in procyanidin C1 compared with control samples. The AA retention following HIU treatments was 87.62-102.86% compared to 30.47-61.90% in thermally treated samples. Our analyses portrayed ultrasound as a feasible alternative to conventional thermal processing of BFP.


Asunto(s)
Adansonia/química , Inhibidores Enzimáticos/farmacología , Frutas/química , Ultrasonido/métodos , alfa-Amilasas/antagonistas & inhibidores , Antioxidantes/análisis , Antioxidantes/química , Ácido Ascórbico/análisis , Biflavonoides/análisis , Catequina/análisis , Inhibidores Enzimáticos/análisis , Inhibidores Enzimáticos/química , Flavonoides/análisis , Inhibidores de Glicósido Hidrolasas/análisis , Inhibidores de Glicósido Hidrolasas/química , Inhibidores de Glicósido Hidrolasas/farmacología , Fenoles/análisis , Proantocianidinas/análisis , alfa-Glucosidasas/metabolismo
14.
J Pharm Biomed Anal ; 201: 114139, 2021 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-34000580

RESUMEN

Baobab fruit pulp Adansonia digitata (AD) has received attention due to its numerous nutritional and medicinal values. In the current study, tentative identification was performed due to limited information available on its phytochemical composition. Phytochemicals from AD fruit pulp were obtained using successive organic solvent fractionation. The LC-MSMS analysis led to identification of 91 metabolites from methanol, butanol and ethyl acetate extracts. Moreover, 20 compounds were identified in the petroleum ether extract based on high resolution ion masses. In vitro antidiabetic and antioxidant properties of selected extracts were investigated using enzyme activity and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, respectively. Biological screening of the antidiabetic effects of target extracts was performed against streptozotocin-induced diabetes in experimental animals, following daily oral treatment for 3 successive weeks. Serum glucose, insulin, adiponectin, superoxide dismutase (SOD), lipid peroxide, cholesterol and HDL levels were measured. Finally, histopathological and immunohistochemical examinations of pancreas were carried out. Results revealed that animal groups treated daily with butanol (BuOH) and petroleum ether extracts of AD (oil) exhibited a significant improvement in carbohydrate and lipid metabolism as well as antioxidant effect. Both extracts revealed superior effects with respect to the total (TT) and ethyl acetate (EtOAc) extracts. Histopathological and immunohistochemical findings supported these results, showing marked protection of the pancreas. Thus, baobab oil and butanolic extract of the fruit pulp protected animals against STZ-induced diabetic changes, in addition to attenuation of lipid peroxidation, hypercholesterolemia and oxidation.


Asunto(s)
Adansonia , Diabetes Mellitus Experimental , Animales , Antioxidantes/farmacología , Diabetes Mellitus Experimental/tratamiento farmacológico , Frutas , Lipidómica , Extractos Vegetales/farmacología , Ratas
15.
Food Chem ; 361: 130080, 2021 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-34029894

RESUMEN

Evaluation of 100 Indian Musa accessions (IMA) for nine elements in their fresh fruit pulp (FFP) revealed genetic variability of 4.7-fold for K & Mg to 111.1-fold for Ca but, only with either highly or moderately positively skewed distribution. The descending order of mineral concentrations (MC) was K > Ca > Na > Mg > Fe > Mn > B > P > Zn. 100 g FFP contributes fairly about 5 (Fe) to 10% (Mn, Ca & Mg) of daily mineral requirement of Indians. Calcium (97%) and Fe (96%) showed the highest heritability while Zn exhibited lowest (85%). Significantly positive correlation was observed for all minerals. Magnesium had maximum direct effect on Fe content followed by Mn, Zn and Na in path analysis. Both principal component analysis and cluster analysis failed to group the IMA according to their ploidy/genome/subgroups. Twenty commercial cultivars were placed in top 10 positions based on their MC. Besides Ca and Mg, IMA were richer for all micronutrients than the world's Musa gene-pool.


Asunto(s)
Musa/química , Frutas/anatomía & histología , Frutas/química , Frutas/genética , Variación Genética , Micronutrientes/análisis , Minerales/análisis , Musa/anatomía & histología , Musa/genética
16.
Arch Microbiol ; 203(6): 3331-3339, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-33866380

RESUMEN

Interest in the production of exopolysaccharides by microorganisms has increased in the recent years. Using low-cost product is the main step of microbial production to reduce cost and compete with chemical production. In this work, EPS production of Streptococcus thermophilus isolates from yogurt (S2), kefir (S3), and S. thermophilus ATCC 19258 (S1) isolate which was used as control strains were investigated by using different fruit pulps. S. thermophilus isolates were identified by morphological and 16S sequence analysis. The amount of EPS obtained was measured spectrophotometrically using glucose as standard with phenol sulfuric acid method. All three isolates produced higher amounts of EPS on M17 medium than Nutrient medium. When the fruit pulp was added to the medium, EPS production increased in all three isolates. When different nitrogen sources were added together with fruit pulp juice, EPS production increased. The highest amount of EPS produced by ATCC 19258 strain (21.570 mg/L) and S3 isolate (29.131 mg/L) is the medium where mixed fruit pulp juice and nitrogen source is tryptophan. It has been shown that EPS production is increased by adding fruit pulps to the prepared media. It is thought that apricot pulp can be a good alternative in EPS production especially in the evaluation of wastes. Also, antiquorum sensing activity of the highest amount EPS was determined by using Chromobacterium violaceum CV026 strain and found effective on violacein pigment inhibition and C6-AHL production of biosensor strain.


Asunto(s)
Polisacáridos Bacterianos/biosíntesis , Streptococcus thermophilus/metabolismo , Chromobacterium/metabolismo , Chromobacterium/fisiología , Medios de Cultivo , Indoles/metabolismo , Percepción de Quorum , Streptococcus thermophilus/aislamiento & purificación
17.
Int J Biol Macromol ; 167: 85-92, 2021 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-33249156

RESUMEN

Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructose-rich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7 ± 0.1 mol% 3-hydroxydecanoate, 17.9 ± 1.0 mol% 3-hydroxyoctanoate, 14.5 ± 1.1 mol% 3-hydroxybutyrate, 11.1 ± 0.6 mol% 3-hydroxytetradecanoate, 10.1 ± 0.5 mol% 3-hydroxydodecanoate and 3.7 ± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (-40.9 ± 0.7 °C and 42.0 ± 0.1 °C, respectively), and a degradation temperature of 300.0 ± 0.1 °C, coupled to a low crystallinity index (12.7 ± 2.7%), a molecular weight (Mw) of 1.34 × 105 ± 0.18 × 105 Da and a polydispersity index of 2.70 ± 0.03. The biopolymer's films were dense and had a smooth surface, as demonstrated by Scanning Electron Microscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging.


Asunto(s)
Biopolímeros/química , Fermentación , Frutas/química , Polihidroxialcanoatos/biosíntesis , Pseudomonas/metabolismo , Residuos , Reactores Biológicos , Fraccionamiento Químico , Fenómenos Químicos , Cinética , Malus , Fenómenos Mecánicos , Peso Molecular , Polihidroxialcanoatos/química , Polihidroxialcanoatos/aislamiento & purificación
18.
Artículo en Inglés | LILACS-Express | LILACS | ID: biblio-1437972

RESUMEN

Cerrado is the second largest phytogeographic domain in Brazil, with a huge ethnobotany variety, including fruit species that stand out for their economic, industrial, biotechnological and medicinal potential. The objective of this study was to characterize the diversity of culturable yeasts and their potential for the production of hydrolytic enzymes in fruits of 13 species of native plants of the Cerrado in Brazil. Sequencing the 26S rRNA gene identified the isolates. The enzymatic potential was evaluated using specific substrates for the enzymes amylases, cellulases, proteases, and pectinases. Nine of the 13 fruit species analyzed showed yeast growth, totaling 82 isolates, identified in 26 species. The phylum Ascomycota predominated over Basidiomycota. The fruits of Butia capitata presented the highest species richness. Candida and Meyerozyma were the most frequent genera. About 57% of the isolates were able to produce at least one of the enzymes analyzed. The species Papiliotrema flavescens, Hanseniaspora meyeri, Meyerozyma guilliermondii, and Rhodotorula mucilaginosa produced all the enzymes tested. The results were found to expand the knowledge about the yeast communities present in fruits of the Cerrado native plants, evidencing the presence of species shared among the plants, and their potential for biotechnological use in the future.


O Cerrado é o segundo maior domínio fitogeográfico do Brasil, com grande variedade etnobotânica, incluindo espécies frutíferas que se destacam por seu potencial econômico, industrial, biotecnológico e medicinal. O objetivo deste trabalho foi caracterizar a diversidade de leveduras cultiváveis e seu potencial para a produção de enzimas hidrolíticas em frutos de 13 espécies de plantas nativas do Cerrado brasileiro. O sequenciamento do gene 26S rRNA identificou os isolados. O potencial enzimático foi avaliado utilizando substratos específicos para as enzimas amilases, celulases, proteases e pectinases. Nove das 13 espécies de frutos analisadas apresentaram crescimento de levedura, totalizando 82 isolados, identificados em 26 espécies. O filo Ascomycota predominou sobre Basidiomycota. Os frutos de Butia capitata apresentaram a maior riqueza de espécies. Candida e Meyerozyma foram os gêneros mais frequentes. Cerca de 57% dos isolados foram capazes de produzir pelo menos uma das enzimas analisadas. As espécies Papiliotrema flavescens, Hanseniaspora meyeri, Meyerozyma guilliermondii e Rhodotorula mucilaginosa produziram todas as enzimas testadas. Os resultados encontrados ampliam o conhecimento sobre as comunidades de leveduras presentes nos frutos das plantas nativas do Cerrado, evidenciando a presença de espécies compartilhadas entre as plantas, e seu potencial para uso biotecnológico no futuro.

19.
J Texture Stud ; 51(6): 937-947, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-32743832

RESUMEN

Reverse spherification is a common technique used in molecular gastronomy to produce innovative products with an improved texture by shaping a liquid into an edible semisolid sphere that gives a burst in the mouth sensation. In this study, liquid-core hydrogel beads (LHBs) were prepared using Syzygium caryophyllatum fruit pulp adapting reverse-phase molecular gastronomy as a minimal processing technique to promote it as a healthy snack. Three types of hydrogel beads were formulated while considering the stability of LHBs. Long-term hardening of fruit juice in sodium alginate solution and the addition of plasticizer was used as two methods to increase the textural stability of LHBs. Results revealed that the addition of the plasticizer imparted to improve all the physical and textural properties of beads; however, it affects the transparency of the hydrogel membrane as well. Although the plasticizer increased the textural stability of LHBs, prolong inlaying them in it (the plasticizer) contribute to occur adverse consequences on the quality. Hence, the inlaying of LHBs in glycerol for 2 min was selected as the best treatment (HBP1). Since HBP1 had a low hardness (125.00 g) and high resilience (0.21), it imparted a chewing gum-like texture to LHBs. Hence, it (HBP1) can be used as a healthy snack. While HBP1 was capable of retained 90% antioxidant activity of fresh fruit of S. caryophyllatum, total polyphenolic content, 2,2-diphenyl-2-picryl-hydrazyl scavenging activity %, and ferric reducing antioxidant power value of this formulation were 59.50 GAE/g of dried LHBs, 68.96% and 139.69 TE/g of dried LHBs, respectively.


Asunto(s)
Antioxidantes/química , Frutas/química , Hidrogeles/química , Bocadillos , Syzygium/química , Alginatos , Antioxidantes/análisis , Compuestos de Bifenilo , Hidrogeles/análisis , Extractos Vegetales
20.
J Sci Food Agric ; 100(15): 5596-5602, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32608520

RESUMEN

BACKGROUND: A lack of knowledge about the dynamic rheological properties and color degradation of bael fruit pulp gives rise to many problems during thermal processing for the preparation of bael fruit products. In this research, the effects of processing temperature (30-75 °C) on dynamic rheology and color degradation kinetics of bael fruit pulp were studied and analyzed. RESULTS: Bael fruit pulp showed more elastic behavior than viscous behavior by analyzing the storage modulus, loss modulus and phase angle over oscillatory frequency range 0.06-300 rad s-1 . The dynamic rheological study classified the bael fruit pulp as a weak gel (G' > G″). The values of first-order degradation rate constant (k) were determined to be 0.07, 0.011, 0.018 and 0.023 for 30, 45, 60 and 75 °C, respectively. The first-order color degradation kinetics of bael fruit pulp has given a higher value of activation energy (Ea = 23.83 kJ mol-1 ) with coefficient of determination (R2 ) of 0.99, which indicated a higher sensitivity for treatment temperature and time during processing. CONCLUSIONS: The results obtained would allow simplification of processes for easier formulation of quality products from bael fruit pulp. This study has the potential to increase the processing ability of bael fruit, which will encourage a greater production of bael fruits. © 2020 Society of Chemical Industry.


Asunto(s)
Aegle/química , Manipulación de Alimentos/métodos , Frutas/química , Pigmentos Biológicos/química , Color , Geles/química , Cinética , Reología , Temperatura
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