RESUMEN
PURPOSE: Since the enactment of the long-term care insurance (LTCI) act in 2000, the number of LTCI users has increased annually. However, evidence regarding what is being carried out as rehabilitation treatment under LTCI is lacking. In this study, a scoping review was performed to bridge this knowledge gap. METHODS: Articles related to rehabilitation in connection with LTCI published between April 2000 and November 2020 were searched for in PubMed, CINAHL, CENTRAL (Cochrane Central Register of Controlled Trials), Ichushi Web Ver.5, and CiNii and randomized controlled trials (RCTs) of rehabilitation provided under LTCI were examined. RESULTS: Of the 15,572 publications identified, 15 RCTs, including rehabilitation treatment by physiatrists and therapists, met the eligibility criteria of our review and were included. The rehabilitation trials in the 15 RCTs varied and included balance training, exercise therapy, cognitive tasks, and activities such as singing and dancing. The results allowed us to focus on three categories: fall prevention, dementia, and theory and tools interventions related to occupational therapy practice. CONCLUSION: The focal points of attention in the rehabilitation treatment of LTCI were identified. However, the physical function, quality of life, and activities of daily living (ADL) of those who "need support" vary from person to person. Therefore, the consolidation of evidence on rehabilitation treatment of LTCI must be continued.
RESUMEN
Phospholipase D, with a molecular mass of 64 kDa, was purified from the psychrophile, Shewanella sp. The enzyme showed maximal activity at pH 7.8 and 40 degrees C in the presence of the Ca2+-ion, and its activity at 10 degrees C was 6.5% of maximum. The enzyme exhibited high activity to the non-micelle form of phosphatidylcholine in an aqueous solution containing water miscible alcohols such as methanol, ethanol, iso-propanol, and n-propanol. Nucleotide sequencing of the enzyme gene yielded a deduced amino acid sequence, which showed 36.2% identity to that of Streptomyces chromofuscus phospholipase D alone. The low sequence similarity to other phospholipase D enzymes suggests that the purified enzyme might be a novel phospholipase D.
Asunto(s)
Clonación Molecular , Genes Bacterianos , Fosfolipasa D/genética , Shewanella/enzimología , Secuencia de Aminoácidos , Secuencia de Bases , Estabilidad de Enzimas , Expresión Génica , Concentración de Iones de Hidrógeno , Cuerpos de Inclusión/química , Metales Pesados/farmacología , Datos de Secuencia Molecular , Peso Molecular , Fosfolipasa D/química , Fosfolipasa D/aislamiento & purificación , Fosfolipasa D/metabolismo , Pliegue de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Shewanella/genética , TemperaturaRESUMEN
Stem cells are thought to inhabit in a unique microenvironment, known as "niche," in which they undergo asymmetric cell divisions that results in reproducing both stem cells and progenies to maintain various tissues throughout life. The cells of osteoblastic lineage have been identified as a key participant in regulating the number of hematopoietic stem cells (HSCs). HSCs receive their regulatory messages from the microenvironment in the bone marrow. This would account for a reason why the localization of hematopoiesis is usually restricted in the bone marrow. To clarify the above possibility we employed a cell implantation-based strategy with a unique osteoblast cell line (KUSA-A1) derived from a C3H/He mouse. The implantation of KUSA-A 1 cells resulted in the generation of ectopic bones in the subcutaneous tissues of the athymic BALB/c nu/nu mice. Subsequently the mice obtained a greater amount of the bone marrow than normal mice, and they showed an increased number of HSCs. These results indicate that the newly generated osteoblasts-derived ectopic bones are responsible for the increase in the number of the HSC population. Furthermore, the increased number of HSCs directly correlates with both the magnitude of dynamic osteogenic process and the size of the newly generated bone or "niche."
Asunto(s)
Movimiento Celular/fisiología , Células Madre Hematopoyéticas/citología , Células Madre Hematopoyéticas/fisiología , Osteogénesis/fisiología , Bazo/citología , Bazo/fisiología , Células Madre/fisiología , Animales , Antígenos Ly/análisis , Células de la Médula Ósea/citología , Células de la Médula Ósea/fisiología , Huesos , Recuento de Células , Línea Celular , Células Cultivadas , Coristoma/inmunología , Coristoma/patología , Coristoma/fisiopatología , Citocinas/metabolismo , Fémur/citología , Fémur/inmunología , Fémur/fisiología , Citometría de Flujo , Regulación de la Expresión Génica , Hematopoyesis/fisiología , Células Madre Hematopoyéticas/química , Células Madre Hematopoyéticas/inmunología , Complejo Mayor de Histocompatibilidad/genética , Complejo Mayor de Histocompatibilidad/fisiología , Proteínas de la Membrana/análisis , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Osteoblastos/citología , Osteoblastos/inmunología , Osteoblastos/fisiología , Osteoblastos/trasplante , Proteínas Proto-Oncogénicas c-kit/análisis , Bazo/química , Bazo/inmunología , Tejido Subcutáneo/inmunología , Tejido Subcutáneo/patología , Tejido Subcutáneo/fisiopatología , TrasplantesRESUMEN
We have already reported a method for fabricating ultramicroelectrodes (Suzuki, K. JP Patent, 2004-45394, 2004). This method is based on the selective chemical etching of optical fibers. In this work, we undertake a detailed investigation involving a combination of etched optical fibers with various types of tapered tip (protruding-shape, double- (or pencil-) shape and triple-tapered electrode) and insulation with electrophoretic paint. Our goal is to establish a method for fabricating nanometer-sized optical fiber electrodes with high reproducibility. As a result, we realized pencil-shaped and triple-tapered electrodes that had radii in the nanometer range with high reproducibility. These nanometer-sized electrodes showed well-defined sigmoidal curves and stable diffusion-limited responses with cyclic voltammetry. The pencil-shaped optical fiber, which has a conical tip with a cone angle of 20 degrees , was effective for controlling the electrode radius. The pencil-shaped electrodes had higher reproducibility and smaller electrode radii (r(app) < 1.0 nm) than those of other etched optical fiber electrodes. By using a pencil-shaped electrode with a 105-nm radius as a probe, we obtained simultaneous electrochemical and optical images of an implantable interdigitated array electrode. We achieved nanometer-scale resolution with a combination of scanning electrochemical microscopy SECM and optical microscopy. The resolution of the electrochemical and optical images indicated sizes of 300 and 930 nm, respectively. The neurites of living PC12 cells were also successfully imaged on a 1.6-microm scale by using the negative feedback mode of an SECM.
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Tecnología de Fibra Óptica , Microscopía de Fuerza Atómica/métodos , Microscopía de Sonda de Barrido/métodos , Nanopartículas/química , Animales , Membrana Celular/química , Electroquímica , Electrodos , Microscopía de Fuerza Atómica/instrumentación , Microscopía de Sonda de Barrido/instrumentación , Neuritas/ultraestructura , Fibras Ópticas , Células PC12 , Tamaño de la Partícula , Ratas , Sensibilidad y Especificidad , Propiedades de SuperficieRESUMEN
BACKGROUND: Marked parathyroid hyperplasia with bone diseases and vascular calcification are unsolved issues in dialysis patients. In this study, we made azotemic model rats by adenine feeding and analyzed the development and progression of the abnormalities. METHODS: Renal failure was induced in 8-week-old male Wistar rats by feeding 0.75% adenine-containing diet for 6 weeks. Serum parameters, parathyroid hyperplasia, bone changes and metastatic calcification were examined at 2, 4 and 6 weeks. RESULTS: Progressive increase of serum creatinine and inorganic phosphate, and decreased levels of serum calcium and 1,25(OH)2D3 were confirmed. Markedly enlarged parathyroid glands and extremely high PTH levels were observed in all adenine-fed rats compared with the control (PTH: 199.3+/-58.0 vs 10.5+/-3.0 pmol/l, P<0.01, respectively, at 6 weeks). In cortical bone of the femur, the morphometric parameters showed increased bone resorption with increased fibrosis, whereas in the trabecular bone, bone resorption decreased and bone volume increased with a larger amount of osteoid compared with the control. Metastatic calcification in aorta, coronary artery and other soft tissues were also found in adenine-fed rats. CONCLUSIONS: Uraemic rats made by adenine diet developed severe abnormalities of calcium metabolism in a relatively short period and therefore they may serve as a useful model for the analysis of parathyroid hyperplasia and vascular calcification in chronic renal failure.
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Enfermedades de la Aorta/etiología , Calcinosis/etiología , Trastorno Mineral y Óseo Asociado a la Enfermedad Renal Crónica/etiología , Enfermedad Coronaria/etiología , Hiperparatiroidismo/etiología , Uremia/complicaciones , Adenina/toxicidad , Animales , Enfermedades de la Aorta/metabolismo , Enfermedades de la Aorta/patología , Nitrógeno de la Urea Sanguínea , Calcinosis/sangre , Calcinosis/patología , Calcitriol/sangre , Calcio/sangre , Trastorno Mineral y Óseo Asociado a la Enfermedad Renal Crónica/metabolismo , Trastorno Mineral y Óseo Asociado a la Enfermedad Renal Crónica/patología , Enfermedad Coronaria/sangre , Enfermedad Coronaria/patología , Creatinina/sangre , Modelos Animales de Enfermedad , Ensayo de Inmunoadsorción Enzimática , Fémur/patología , Estudios de Seguimiento , Hiperparatiroidismo/sangre , Hiperparatiroidismo/patología , Masculino , Microrradiografía , Hormona Paratiroidea/sangre , Ratas , Ratas Wistar , Índice de Severidad de la Enfermedad , Uremia/inducido químicamente , Uremia/patologíaRESUMEN
BACKGROUND: Although it effectively suppresses parathyroid hormone (PTH) secretion, vitamin D [1,25(OH)(2)D(3)] therapy often causes tissue calcification over the long term. In patients on chronic dialysis, cardiovascular calcification is clearly linked to an unfavourable prognosis. In pre-dialysis patients, renal calcification of the kidney leads to the deterioration of renal function. METHODS: We compared the propensities of 22-oxacalcitriol (OCT), with lesser calcaemic action, and 1,25(OH)(2)D(3) for producing their potential side effects in rats: (i) metastatic calcification of heart and aorta, and (ii) renal dysfunction with nephrocalcinosis, using the same effective doses for hyperparathyroidism. OCT (1.25 and 6.25 micro g/kg) or 1,25(OH)(2)D(3) (0.125 and 0.625 micro g/kg) solutions were administered intravenously to subtotally nephrectomized (SNX) rats three times weekly for 2 weeks. RESULTS: Despite the suppression of PTH to comparable levels, the calcification of the hearts, aortas and kidneys in the 1,25(OH)(2)D(3)-treated group was significantly greater than in the OCT-treated group. Of interest was that, in the OCT (6.25 micro g/kg) group, the degree of calcification in hearts, aortas and kidneys were distinctly lower than those in the 1,25(OH)(2)D(3) (0.125 micro g/kg) group despite the comparable serum Ca x Pi products. Therefore, there may be different mechanisms behind the calcifications resulting from OCT and 1,25(OH)(2)D(3). Deterioration of renal function, tubular changes, and atypical hyperplasia of proximal tubules associated with calcification were more severe in the 1,25(OH)(2)D(3)-treated group than in the OCT-treated group. CONCLUSIONS: These results indicate that OCT may be an effective agent for the suppression of PTH with a lesser risk of cardiovascular calcification or deterioration of residual renal function.
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Calcinosis/inducido químicamente , Calcitriol/farmacología , Enfermedades Cardiovasculares/inducido químicamente , Enfermedades Renales/inducido químicamente , Hormona Paratiroidea/fisiología , Vitaminas/farmacología , Animales , Calcitriol/análogos & derivados , Sistema Cardiovascular/efectos de los fármacos , Riñón/efectos de los fármacos , Masculino , Nefrectomía , Ratas , Ratas Sprague-DawleyRESUMEN
Controlled regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped-bone with high-density bone trabeculae when used in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA-collagen sponge, show promise for use in a custom-shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications.
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Células de la Médula Ósea/metabolismo , Regeneración Ósea/fisiología , Técnicas de Cultivo de Célula/métodos , Osteoblastos/metabolismo , Células del Estroma/metabolismo , Ingeniería de Tejidos/métodos , Trasplante de Tejidos/métodos , Implantes Absorbibles/tendencias , Animales , Antígenos de Superficie/metabolismo , Materiales Biocompatibles/farmacología , Materiales Biocompatibles/uso terapéutico , Células de la Médula Ósea/citología , Regeneración Ósea/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Diferenciación Celular/fisiología , Colágeno/farmacología , Colágeno/uso terapéutico , Matriz Extracelular/efectos de los fármacos , Matriz Extracelular/metabolismo , Femenino , Genes Reporteros , Ácido Láctico/farmacología , Ácido Láctico/uso terapéutico , Ratones , Ratones Endogámicos C3H , Osteoblastos/citología , Osteogénesis/efectos de los fármacos , Osteogénesis/fisiología , Ácido Poliglicólico/farmacología , Ácido Poliglicólico/uso terapéutico , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Polímeros/farmacología , Polímeros/uso terapéutico , Células del Estroma/citologíaRESUMEN
BACKGROUND: 1,25-Dihydroxy-22-oxavitamin D(3) (22-oxacalcitriol, OCT) is an analogue of 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3), calcitriol) with less calcaemic activity, thus more suitable than 1,25(OH)(2)D(3) for the control of parathyroid hormone (PTH) secretion in chronic dialysis patients. As the low-calcaemic action of OCT has been mainly attributed to its short half-life in the blood stream, the number of doses per week is the key factor to effective OCT therapy toward suppression of PTH secretion and hypercalcaemia. Thus, we investigated a comparison between daily and thrice-weekly i.v. administration of OCT regarding suppression of PTH secretion and calcaemic action in 5/6 nephrectomized rats as a model for chronic renal failure. METHODS: Model rats of chronic renal failure were made by 5/6 nephrectomy. At 3 months after surgery, they were administered either vehicle or OCT intravenously, daily (0.125 or 0.625 microg/kg) or thrice-weekly (0.6 or 3.0 microg/kg) for 2 weeks. RESULTS: The data show that 0.625 microg/kg/day (=4.375 microg/kg/week) suppresses PTH secretion with significant increase in calcium levels at 24 h after the final administration, on the other hand, 3.0 microg/kg/ thrice-weekly (=9.0 microg/kg/week) suppresses PTH secretion, although moderate compared with 0.625 microg/kg/day, with a slight (not significant) increase in calcium. CONCLUSIONS: The current clinical mode of OCT therapy, i.v. thrice-weekly administration, is a practically recommendable protocol.