Your browser doesn't support javascript.
loading
Characterisitics and gene cloning of phospholipase D of the psychrophile, Shewanella sp.
Tsuruta, Hiroki; Hayashi, Ryuhei; Ohkawa, Hiroyuki; Ohkatsu, Nobuhide; Morimoto, Masakazu; Nishimoto, Kaname; Santou, Sigeyuki; Aizono, Yasuo.
Afiliación
  • Tsuruta H; Headquarters for Innovative Cooperation and Development, Kobe University, Japan. tsuruta@kobe-u.ac.jp
Biosci Biotechnol Biochem ; 71(10): 2534-42, 2007 Oct.
Article en En | MEDLINE | ID: mdl-17928713
Phospholipase D, with a molecular mass of 64 kDa, was purified from the psychrophile, Shewanella sp. The enzyme showed maximal activity at pH 7.8 and 40 degrees C in the presence of the Ca2+-ion, and its activity at 10 degrees C was 6.5% of maximum. The enzyme exhibited high activity to the non-micelle form of phosphatidylcholine in an aqueous solution containing water miscible alcohols such as methanol, ethanol, iso-propanol, and n-propanol. Nucleotide sequencing of the enzyme gene yielded a deduced amino acid sequence, which showed 36.2% identity to that of Streptomyces chromofuscus phospholipase D alone. The low sequence similarity to other phospholipase D enzymes suggests that the purified enzyme might be a novel phospholipase D.
Asunto(s)
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolipasa D / Clonación Molecular / Shewanella / Genes Bacterianos Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolipasa D / Clonación Molecular / Shewanella / Genes Bacterianos Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2007 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido