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Use of isolated mature osteoblasts in abundance acts as desired-shaped bone regeneration in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge.
Ochi, Kensuke; Chen, Gouping; Ushida, Takashi; Gojo, Satoshi; Segawa, Kaoru; Tai, Hitoshi; Ueno, Kenju; Ohkawa, Hiroyuki; Mori, Taisuke; Yamaguchi, Akira; Toyama, Yoshiaki; Hata, Jun-Ichi; Umezawa, Akihiro.
Afiliación
  • Ochi K; Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
J Cell Physiol ; 194(1): 45-53, 2003 Jan.
Article en En | MEDLINE | ID: mdl-12447988
Controlled regeneration of bone or cartilage has recently begun to facilitate a host of novel clinical treatments. An osteoblast line, which we isolated is able to form new bone matrix in vivo within 2 days and exhibits a mature osteoblast phenotype both in vitro and in vivo. Using these cells, we show that cuboidal bones can be generated into a predesigned shaped-bone with high-density bone trabeculae when used in combination with a modified poly-DL-lactic-co-glycolic acid (PLGA)-collagen sponge. PLGA coated with collagen gel serves as a good scaffold for osteoblasts. These results indicate that mature osteoblasts, in combination with a scaffold such as PLGA-collagen sponge, show promise for use in a custom-shaped bone regeneration tool for both basic research into osteogenesis and for development of therapeutic applications.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Regeneración Ósea / Células de la Médula Ósea / Trasplante de Tejidos / Células del Estroma / Técnicas de Cultivo de Célula / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2003 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Regeneración Ósea / Células de la Médula Ósea / Trasplante de Tejidos / Células del Estroma / Técnicas de Cultivo de Célula / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2003 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos