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Extruded spaghetti-type pasta systems were obtained separately either from native or oxidized starch prepared via wet chemistry with the aim of evaluating the effect of oxidation modification of starch. In addition to this, the butyrylation reaction (butyrate (Bu) esterification-short-chain fatty acid) using native or oxidized starch was analyzed under reactive extrusion (REx) conditions with and without the addition of a green food-grade organocatalyst (l(+)-tartaric acid) with the purpose of developing potentially health-promoting spaghetti-type pasta systems in terms of increasing its resistant starch (RS) values. These would be due to obtaining organocatalytic butyrylated starch or not, or the manufacture of a doubly modified starch (oxidized-butyrylated-starch oxidation followed by organocatalytic butyrylation) or not. To this end, six pasta systems were developed and characterized by solid-state 13C cross-polarization magic angle spinning nuclear magnetic resonance (CP MAS NMR) spectroscopy, degree of substitution (DS), attenuated total reflectance Fourier transform infrared (ATR/FTIR) spectroscopy, X-ray diffraction (XRD), thermogravimetric analysis (TGA), pancreatic digestion, free Bu content analysis and in vitro starch digestibility. The results obtained here suggest that starch oxidation hydrolytically degrades starch chains, making them more susceptible to enzymatic degradation by α-amylase. However, the oxidized starch-based pasta systems, once esterified by Bu mainly on the amylose molecules (doubly modified pasta systems) increased their RS values, and this was more pronounced with the addition of the organocatalyst (maximum RS value = ~8%). Interestingly, despite the checked chemical changes that took place on the molecular structure of starch upon butyrylation or oxidation reactions in corn starch-based spaghetti-type pasta systems, and their incidence on starch digestibility, the orthorhombic crystalline structure (A-type starch) of starch remained unchanged.

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Polymeric anthocyanins are biologically active, pH-sensitive natural compounds and pigments with beneficial functional, pharmacological and therapeutic properties for consumer health. More recently, they have been used for the manufacture of active and pH-sensitive ("intelligent") food nanopackaging, due to their bathochromic effect. Nevertheless, in order for polymeric anthocyanins to be included either as a functional food or as a pharmacological additive (medicinal food), they inevitably need to be stabilized, as they are highly susceptible to environmental conditions. In this regard, nanopackaging has become a tool to overcome the limitations of polymeric anthocyanins. The objective of this study was to evaluate their structural, thermal, morphological, physicochemical, antioxidant and antimicrobial properties, as well as their responses to pH changes, and the cytotoxicity of blends made from polymeric anthocyanins extracted from Jamaica flowers (Hibiscus sabdariffa) and natural or organo-modified montmorillonite (Mt), as active and pH-sensitive nanopackaging. This study allowed us to conclude that organo-modified Mts are efficient pH-sensitive and antioxidant nanopackaging systems that contain and stabilize polymeric anthocyanins compared to natural Mt nanopackaging and stabilizing polymeric anthocyanins. However, the use of these polymeric anthocyanin-stabilizing organo-modified Mt-based nanopackaging systems are limited for food applications by their toxicity.

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Metal-organic frameworks (MOFs) are high surface-to-volume ratio crystalline hybrid porous coordination materials composed of metal ions as nodes and organic linkers. The goal of this paper was to provide an updated and comprehensive state-of-the-art review of MOFs for different food applications such as active food contact materials, antimicrobial nanocarriers, controlled release nanosystems for active compounds, nanofillers for food packaging materials, food nanoreactors, food substance nanosensors, stabilizers and immobilizers for active compounds and enzymes, and extractors of food contaminants. Extraction and sensing of several food contaminants have been the main food applications of MOFs. The other applications listed above require further investigation, as they are at an early stage. However, interesting results are being reported for these other fields. Finally, an important limitation of MOFs has been the use of non-renewable feedstocks for their synthesis, but this has recently been solved through the manufacture and use of γ-cyclodextrin-based MOFs.

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The aim of this research work was to investigate novel tools given by nanotechnology and green chemistry for improving the disadvantages typically associated to the starch-based films: water susceptibility and brittle mechanical behavior. With this in mind, four food packaging film systems were developed from corn starch or corn starch nanocrystals (SNCs), and modified by phosphating under reactive extrusion (REx) conditions using sodium tripolyphosphate (Na5P3O10 - TPP) as a crosslinker. The structural, physicochemical, thermal, rheological and mechanical properties, as well as studies associated with the management of carbohydrate polymer-based plastic wastes (biodegradability and compostability) were carried out in this study. The hierarchical structure and the modification of the starch were dependent on the amylose content and degree of substitution (DS), which in turn depended on the hydrogen (H)-bonding interactions. In both cases, a higher molecular ordering of the starch chains in parallel was decisive to obtain the self-assembled thermoplastic starches. Beyond the valuable results obtained and scientifically analyzed, unfortunately none of the manufactured materials achieved to improve their performance compared to the control film (thermoplastic starch - TPS). It was even thought that the phosphated starch-based films could fertilize lettuce (Lactuca sativa) seedlings during their biodegradation, and this was not achieved either. This possibly due to the low content of phosphorus or its poor bioavailability.

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Lipids play an important role in human nutrition. Several foodstuffs can be manufactured from the simple, compound and derived lipids. In particular, the use of self-assembled lipids (SLs, e.g. self-assembled L-α-lecithin) has brought great attention for the development of tailored, tuned and targeted colloidal structures loading degradation-sensitive substances with valuable antimicrobial, antioxidant and nutraceutical properties for food applications. For example, polyunsaturated fatty acids (PUFAs) and essential oils can be protected from degradation, thus improving their bioavailability in general terms in consumers. From a nanotechnological point of view, SLs allow the development of advanced and multifaceted architectures, in which each molecule of them are used as building blocks to obtain designed and ordered structures. It is important to note before beginning this review, that simple and compound lipids are the main SLs, while essential fatty acids and derived lipids in general have been considered by many research groups as the bulk loaded substances within several structures from self-assembled carbohydrates, proteins and lipids. However, this review paper is addressed on the analysis of the lipid-lipid self-assembly. Lipids can be self-assembled into various structures (micelles, vesicular systems, lyotropic liquid crystals, oleogels and films) to be used in different food applications: coatings, controlled and sustained release materials, emulsions, functional foods, etc. SLs can be obtained via non-covalent chemical interactions, primarily by hydrogen, hydrophilic and ionic bonding, which are influenced by the conditions of ionic strength, pH, temperature, among others. This manuscript aims to give an analysis of the specific state-of-the-art of SLs for food applications, based primarily on the literature reported in the past five years.

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Electroactive scaffolds derived from carbohydrate hydrogels were synthesized, resulting in a large shift in the conductivity of chitosan (CS) from 10-6 S/cm to about 10-3 S/cm, assigned to CS-oligoaniline. Several analyses including UV-vis spectroscopy and cyclic voltammetry were performed, before examining the carbohydrate-based scaffolds for their ability to recapitulate the neural tissue microenvironment. Good conductivity and resemblance of the modulus to soft tissue of the optimized hydrogels led to appropriate cellular activity and neural regeneration. The loss of dopaminergic neurons as the prominent source of dopamine in the central nervous system results in the deterioration of multiple brain functions such as voluntary movement and behavioral processes. To overcome this, olfactory ecto-mesenchymal stem cells (OE-MSCs) were induced to differentiate into dopaminergic neuron-like cells on hydrogels through a monolayer arrangement cell culture by using cocktail neurotrophic factors including sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), basic fibroblast growth factor (bFGF), glial cell line-derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF). The differentiation capacity of a series of OE-MSCs on the conductive hydrogel was evaluated by real-time PCR, immunocytochemistry and flow cytometry, and the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) neural and dopaminergic markers. The results of this study represent the first steps in designing and implementing advanced platforms based on conductive polysaccharide hydrogels for neural disorder therapies, such as the treatment of Parkinson's disease.

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The self-assembled natural and synthetic polymers are booming. However, natural polymers obtained from native or modified carbohydrate polymers (CPs), such as celluloses, chitosan, glucans, gums, pectins, and starches, have had special attention as raw material in the manufacture of self-assembled polymer composite materials having several forms: films, hydrogels, micelles, and particles. The easy manipulation of the architecture of the CPs, as well as their high availability in nature, low cost, and being sustainable and green polymers have been the main positive points in the use of them for different applications. CPs have been used as building blocks for composite structures, and their easy orientation and ordering has given rise to self-assembled CPs (SCPs). These macromolecules have been little studied for food applications. Nonetheless, their research has grown mainly in the last 5 years as encapsulated food additive wall materials, food coatings, and edible films. The multifaceted properties (systems sensitive to pH, temperature, ionic strength, types of ions, mechanical force, and enzymes) of these devices are leading to the development of advanced food materials. This review article focused on the analysis of SCPs for food applications in order to encourage other research groups for their preparation and implementation.

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Plantain flour (Musa ssp., group AAB, sub-group clone Harton) was modified by acetylation, carboxymethylation, methylation, oxidation and phosphation, in order to determine which of these modifications produce a material with potential for the development of gluten-free products with improved nutritional properties. The structural, rheological and nutritional properties of the modified flours were investigated. The phosphated plantain flour, cross-linked with sodium trimetaphosphate (STMP), had a lower in vitro digestibility rate associated with resistant starch (RS) types RS2, RS4 and RS5. This latter was confirmed by the formation of the amylose-lipid complex as determined by XRD. All the flours tested had a C-type structure with the exception of the methylated plantain flour which produced a large quantity of reaction by-product (BaSO4). The phosphated plantain flour represents a raw material with great potential for the development of gluten-free food (bread and cookie) with functional properties, i.e. health benefits.

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A non-conventional starch obtained from guinea arrowroot tubers (Calathea allouia) grown in the Amazon was used as a polymeric matrix for the development of edible films. The films were manufactured by blending/thermo molding and plasticized with glycerol. Agro-industrial wastes from wine manufacture (grape waste flour and grape waste extract) were used as natural fillers of the thermoplastic starch (TPS) matrices. The results showed that the natural fillers caused cross-linking in the TPS matrix. This led to the production of films with higher resistant starch (RS) content, especially RS type 4 (RS4), although the DSC results showed that the films developed also contained RS type 3 (RS3). As expected, the presence of RS reduced the in vitro digestibility rate. Films made with the natural fillers were also less hydrophilic, had a greater thermal resistance, and tended towards ductile mechanical behavior. Finally, the edible film containing grape waste flour as a natural filler proved to be pH-sensitive, although this material disintegrated under alkaline conditions.

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Plantain flour (Musa ssp., group AAB, sub-group clone Harton)/poly(ε-caprolactone) (PCL) blends, containing glycerol as a plasticizer, were prepared by reactive extrusion (REx) in a twin-screw extruder using zirconium octanoate (Zr(Oct)4) as a catalyst, followed by thermo-compression molding for film development. The films were then characterized in terms of their: infrared (FTIR) spectra, water solubility, thermogravimetric (TGA) curves, differential scanning calorimetry (DSC) thermograms, and X-ray diffraction (XDR) diffractograms, as well as their microstructural, mechanical and antimicrobial properties in order to (1) compare the effects of PCLs with two different molecular weights (Mw) on the characteristics of the plantain flour/PCL blends, and (2) determine whether using Zr(Oct)4 in the production of active composite polymer materials improves their properties. The plantain flour/PCL blends were all developed successfully. The higher Mw PCL gave more hydrophobic and thermally stable films with improved mechanical properties. The addition of the Zr(Oct)4 catalyst to the plantain flour/PCL blends also resulted in films with similar characteristics to those described above, due to the cross-linking of the polymers. In addition, the films containing the catalyst showed antimicrobial activity against Escherichia coli O157:H7 and Staphylococcus aureus indicating a dual effect of Zr(Oct)4, and making it an attractive alternative for the development of active films.

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The data given below relates to the research paper entitled: "Eco-friendly films prepared from plantain flour/PCL blends under reactive extrusion conditions using zirconium octanoate as a catalyst", recently published by our research group [1]. This article provides information concerning the physicochemical properties of the above-mentioned film systems: thickness, density, opacity, moisture content and surface moisture.

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The surface and nutraceutical properties have been poorly studied on edible films. The aim of this study was to investigate the surface properties and potential health effects in terms of in vitro digestibility and anti-inflammatory activity. The materials were developed from native plantain starch and pre-gelatinized plantain flour with and without added blackberry pulp using casting methodology. Thermogravimetric analysis, contact angle, scanning electron microscopy, atomic force microscopy, resistant starch, in vitro digestibility, cell viability, reactive oxygen species, anti-inflammatory activity and sensory evaluation were the tests carried out in this study. Films containing blackberry pulp had more compact and smooth morphologies, which were related to the lower in vitro digestibility rate and the higher resistant starch content. In addition, these materials had higher anti-inflammatory activity, higher cell viability, and better acceptance by the panelists, thus suggesting potential health effects of consumers with special feeding regimes such as obese, diabetics and celiacs.

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The aroma, taste, shine, snap, smoothness, "melt-in-your-mouth" sensation, and texture are all qualities that define chocolate, and all depend on how the cocoa and the chocolate itself are processed. Postharvest handling of the cocoa (fermentation, drying, cleaning, storage, and transport) and its transformation into chocolate (roasting, grinding, conching, tempering, molding, and the addition of core and other ingredients), as well as the packaging, storage, transport, and refrigeration of the finished product all have an important influence on the characteristics of chocolate. The aim of this review was to identify and study the key factors, including microbiological aspects that affect the quality of chocolate, from harvesting the beans right up to the manufacture of the finished products.

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Biodegradable and edible cassava starch-glycerol based films with different concentrations of yerba mate extract (0, 5 and 20wt.%) were prepared by casting. The plasticizing effect of yerba mate extract when it was incorporated into the matrix as an antioxidant was investigated. Thermal degradation and biodegradability of the obtained biofilms were also studied. Thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), attenuated total reflectance Fourier transform infrared spectroscopy (ATR/FTIR), X-ray diffraction analysis (XRD), water absorbance, stability in different solutions and biodegradability studies were performed. The clear correlation among the results obtained from the different analysis confirmed the plasticizing effect of yerba mate extract on the starch-glycerol matrix. Also, the extract led to a decrease in the degradation time of the films in soil ensuring their complete biodegradability before two weeks and to films stability in acidic and alkaline media. The plasticizing effect of yerba mate extract makes it an attractive additive for starch films which will be used as packaging or coating; and its contribution to an earlier biodegradability will contribute to waste reduction.

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Biological macromolecules such as starches of different amylaceous sources have been used in the formulation of edible films. However, there are few studies aimed at evaluating edible and intelligent films with response to pH changes from natural pigments, this despite the importance of these materials. In this context, films from native and modified plantain flour, plasticized with glycerol, with or without the addition of beet flour were developed. The chemical and structural composition of the flours, and its incidence on thickness, water solubility, contact angle, and mechanical and microstructural properties were evaluated, thus as its response to pH changes of the developed films. The observations showed that the incorporation of beet flour allowed to obtain intelligent films front to pH changes alkaline. Likewise, the betalains that were found in beet flour interacted more efficiently with the phosphated plantain flour, limiting well its immediate response to pH changes. In the same way, proteins and sugars of beet flour allowed to obtain more flexible films, due to the hydrogen bond interactions between these constituents and the plantain flours. This latter could justify the decrease of contact angle, and the increase on thickness and solubility of these films.

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