RESUMO

Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avian myeloblastosis virus (AMV) reverse transcriptase, in the presence of polyribonucleotides and 2'-fluorinated polynucleotides as templates. Polynucleotides differentially inhibited the reactions catalyzed by reverse transcriptase in the presence of these synthetic templates. Polyriboadenylic acid (poly(rA), poly(2'-O-methyladenylic acid) (poly(Am)), poly(2'-fluoro-2'-deoxyadenylic acid) (poly(dAfl), polyinosinic acid (poly(rI)) and polyuridylic acid poly(rU)) inhibited the polyribonucleotide-, but not the 2'-fluorinated polynucleotide-directed reverse transcriptase activity.

Assuntos

DNA/biossíntese , Polidesoxirribonucleotídeos/metabolismo , Polinucleotídeos/farmacologia , Polirribonucleotídeos/metabolismo , DNA Polimerase Dirigida por RNA/metabolismo , Vírus da Mieloblastose Aviária/enzimologia , Relação Dose-Resposta a Droga , Flúor/farmacologia , Manganês/farmacologia , Poli A/farmacologia , Poli I/farmacologia , Poli U/farmacologia , Inibidores da Transcriptase Reversa , Moldes Genéticos