RESUMO

This study aimed to evaluate the effect of extrusion and of open-pan cooking on whole germinated and non-germinated grains of pearl millet (Pennisetum glaucum L. R. Br.), on its chemical-nutritional composition and in vitro iron bioavailability. The experimental design consisted of three flours: non-germination open-pan cooked millet flour (NGOPCMF), germination open-pan cooked millet flour (GOPCMF), and extrusion cooked millet flour (ECMF). The ECMF increased the carbohydrates, iron, manganese, diosmin, and cyanidin and decreased the total dietary fiber, resistant starch, lipids, and total vitamin E, in relation to NGOPCMF. The GOPCMF increased the lysine and vitamin C and decreased the phytate, lipids, total phenolic, total vitamin E, and riboflavin concentration, in relation to NGOPCMF. Furthermore, germinated cooked millet flour and extruded millet flour improved iron availability in vitro compared to non-germinated cooked millet flour. GOPCMF and ECMF generally preserved the chemical-nutritional composition of pearl millet and improved in vitro iron bioavailability; therefore, they are nutritionally equivalent and can be used to develop pearl millet-based products.

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Millet is a promising cereal with high amounts of dietary fibre and protein, and in addition, bioactive compounds with health-promoting functional properties. This study aimed to evaluate the effect of germinated and cooked whole millet flour (Pennisetum glaucum (L.) R. Br.) on protein quality, biochemical profile and intestinal health in vivo. Thirty-two male Wistar rats (21 days old) were separated into four groups, which received a casein control diet (CC; n = 8), a free protein diet (aproteic; n = 8) and two treatment diets: non-germinated millet (NM; n = 8) and germinated millet (GM; n = 8) for 29 days. The whole millet flours presented an adequate essential amino acid profile, except for lysine. The GM group presented a higher protein efficiency ratio and net protein ratio compared to the NM group. Weight gain, Lee index, and food efficiency ratio were lower in the treatment groups, compared to the control group. The GM group had lower plasma glucose, uric acid, cholesterol, and faecal pH compared to the other groups. The treatment groups presented lower triglyceride levels, higher levels of acetic and propionic acids, a larger thickness and depth of the colonic crypts, and a higher expression of PepT1 genes than the CC group. In conclusion, the millet flours demonstrated potential for controlling the lipid profile and biometric measurements. Additionally, the whole germinated millet flour provided better protein quality and improved intestinal morphology and functionality. These results indicate that the consumption of millet could be increased in human food, and considering its potential health benefits, it could be an alternative for dietary diversification, and germination is a good processing option.

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This study examined the inclusion of untreated or urea-ammoniated CGT on the chemical composition, pH, gas, and effluent losses, dry matter recovery (DMR), and aerobic stability of elephantgrass silage. The experiment was laid out in a randomized design with a 3 × 3 factorial arrangement represented by three levels of inclusion of CGT during ensiling (0, 5, or 10%) and three levels of ammoniation of CGT with urea before ensiling (0, 4 or 8% for 28 days), using four replications. Data were subjected to analysis of variance and Tukey's test, considering a 5% significance level. The pH and DM, NDIN, ADIN, NDF and ADF contents of the silages increased (p<0.05) with the inclusion of CGT, regardless of ammoniation. The lowest (p<0.05) effluent loss was achieved using 10% of CGT ammoniated with 8% urea. In contrast, the elephantgrass silage exhibited the lowest (p<0.05) gas loss and the greatest (p<0.05) DMR. The inclusion of CGT compromised the aerobic stability of the silage. Therefore, the inclusion of CGT ­either untreated or ammoniated ­in the ensiling of elephantgrass is not recommended.(AU)

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Leucaena has been used to make mixed silages to obtain nutritional enrichment of the silages. Thus, the inclusion of Leucaena as an additive in mixed elephant grass silages can reduce fermentation losses, and increase the nutritional value and aerobic stability of the mixed silage without changing the fermentation profile. This study evaluated the fermentation profile, nutritional composition, and aerobic stability of elephant grass silages combined with different levels of Leucaena. A total of five inclusion levels of Leucaena (0, 20, 40, 60, and 80% on a dry matter basis) were added to elephant grass silages. A completely randomized design was adopted, with 5 treatments and 3 repetitions, totaling 15 experimental silos that were opened after 30 days of sealing. Fermentation profile, chemical composition, and aerobic stability were analyzed. A descriptive analysis of temperature and pH peaks during aerobic stability was performed. The increase in the inclusion of Leucaena in the composition of silages reduced gas and effluent losses, neutral and acid detergent fiber, cellulose, lignin, total and fiber carbohydrates, and total digestible nutrients, and resulted in increased dry matter, ether extract, and crude protein. A quadratic effect of treatments was found for the temperature to reach the maximum pH (P=0.009). Aerobic stability remained constant after 40% Leucaena inclusion in the composition of elephant grass silages. The inclusion of Leucaena up to 80% in the composition of elephant grass silages reduces fermentation losses, promotes a nutritional increase, and increases the aerobic stability of the silages.(AU)

A leucena tem sido utilizada para a confecção de silagens mistas para o enriquecimento nutricional das silagens. Assim, a inclusão da leucena como aditivo em silagens mistas de capim-elefante pode reduzir as perdas fermentativas e aumentar o valor nutricional e a estabilidade aeróbia das silagens, sem alterar o seu perfil fermentativo. Objetivou-se avaliar o perfil fermentativo, composição nutricional e estabilidade aeróbia de silagens de capim elefante associadas com níveis crescentes de leucena. Um total de cinco níveis de leucena (0, 20, 40, 60 e 80% em base da matéria seca) foram incluídos em silagens de capim elefante. Adotou-se um delineamento inteiramente casualizado, com 5 tratamentos e 3 repetições, totalizando 15 silos experimentais, os quais foram abertos após 30 dias de ensilagem. Foram analisados o perfil fermentativo, a composição química e a estabilidade aeróbica. Foi realizada uma análise descritiva dos picos de temperatura e pH durante a estabilidade aeróbia. O aumento da inclusão da leucena na composição das silagens reduziu as perdas por gases, perdas por efluentes, fibra em detergente neutro, fibra em detergente ácido, celulose, lignina, carboidratos totais, carboidratos fibrosos e nutrientes digestíveis totais e aumentou os de teores de matéria seca, extrato etéreo e proteína bruta. Foi observado efeito quadrático dos tratamentos sobre a temperatura para atingir o pH máximo (P=0.009). A estabilidade aeróbia permaneceu constante a partir de 40% de inclusão de leucena na composição das silagens de capim elefante. A inclusão da leucena em até 80% na composição de silagens de capim elefante reduz as perdas fermentativas, promove incremento nutricional e aumento da estabilidade aeróbia das silagens.(AU)

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The objective of this study was to estimate sugar content variation (°Brix) in 95 accessions from the elephant grass germplasm active bank of Embrapa Dairy Cattle research center, located in Coronel Pacheco, MG, Brazil. The accessions with the highest sugar content were identified, and the effect of plant age on sugar concentration was evaluated. The experiment was conducted in randomized blocks design with two replications. Sugar content analysis occurred twice during the growth-cycle (at 70 and 100 days) along two points of the stem, one 30 cm from the base and the other at the midpoint. The analysis of variance considered the split plot model, with accessions as main plots and plant age as subplots. The means were compared using the Scott Knott test. While there was sugar content variation between accessions, increased plant age had no significant effect on the sugar content. However, some accessions did have a significant increase or decrease in sugar content as they aged. The highest and lowest mean sugar content was 6.96% (in the accession BAG80) and 4.03% (in the accession BAG13) °Brix, respectively.(AU)

O objetivo deste estudo foi estimar a variação do teor de açúcar (°Brix) existente entre 95 acessos do banco ativo de germoplasma de capim-elefante da Embrapa Gado de Leite, localizado no campo experimental de Coronel Pacheco. Foram identificados os acessos com maior teor de açúcar, bem como avaliado o efeito da idade da planta sobre a variação da concentração de açúcar. O experimento foi conduzido em blocos ao acaso com duas repetições, 95 genótipos, duas idades de planta, 70 e 100 dias de crescimento, e análise do teor de açúcar em duas secções amostradas à 30 cm da base e na metade do colmo. A análise de variância considerou modelo de parcela subdividida no tempo, sendo os acessos, as parcelas e a idade da planta as subparcelas. As médias foram comparadas pelo teste de Scott Knott. Observou-se variação no teor de açúcar entre os acessos, porém não foi observado efeito significativo no teor de açúcar devido ao aumento da idade da planta. Entretanto, alguns acessos apresentaram interação significativa com aumento ou redução do teor de açúcar em decorrência do aumento da idade da planta. O acesso de maior média de teor de açúcares entre as duas idades foi BAG80, com 6,96%, e o de menor foi BAG13, com 4,03%.(AU)

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The co-digestion of swine manure with vegetable waste is an alternative that can increase the production of biogas and methane generated by the isolated digestion of manure. However, recommendations that are based on the best ratio between manure and forage, as well as the age of harvest, are still scarce in the literature. This study was conducted to evaluate inclusions (0, 25, 50, 75 and 100%) of the total solids (TS) of Elephant grass (Pennisetum purpureum Schum) harvested at two ages medium age (MA) at 45 days of growth and advanced age (AA) at 90 days in co-digestion with swine manure, using an entirely randomized design in a 5x2 factorial scheme. Batch digesters were used and biogas production was monitored for 12 weeks. There was influence of forage age (P <0.05) on the degradation of solids and neutral detergent fiber, with higher values for the substrates containing MA forage. The highest CH4 yields were obtained by the substrates containing MA forage in the inclusion of 27.7 and 31.6%, being 253.7 and 222.2 L of CH4 per Kg of total or volatile solids. The age of the forages influenced the onset and persistence of biogas production, being advantageous only in the inclusion of 25% of MA forage. The AA forage inclusion is not recommended for co-digestion with swine manure.

A co-digestão dos dejetos suínos associados a resíduos vegetais é uma alternativa que pode elevar as produções de biogás e metano alcançadas pela digestão isolada do dejeto. No entanto, recomendações que se baseiem na melhor proporção entre dejeto e forragem, assim como na idade de colheita ainda são escassas na literatura. O objetivo do trabalho foi avaliar inclusões crescentes (0, 25, 50, 75 e 100% dos sólidos totais (ST) de capim elefante (Pennisetum purpureum Schum.) colhido em duas idades de corte mediana (MA) aos 45 dias de crescimento e avançada (AA) aos 90 dias, empregado em co-digestão com o dejeto suíno, adotando-se delineamento inteiramente ao acaso, em esquema fatorial 5x2. Foram utilizados biodigestores batelada de bancada, sendo as produções de biogás acompanhadas durante 12 semanas. Houve influência da idade da forragem (P<0,05) na degradação de sólidos e fibra em detergente neutro, com maiores valores para os substratos que continham a forragem MA. Os maiores rendimentos de CH4 foram alcançados pelos substratos que continham forragem MA na inclusão de 27,7 e 31,6 %, sendo de 253,7 e 222,2 L de CH4 por Kg de ST ou sólido volátil adicionado. A idade das forragens influenciou o início e a persistência das produções de biogás ao longo do período de digestão, sendo vantajosa apenas na inclusão de 25% de forragem com MA.

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O experimento foi realizado no Município de Penápolis/SP, no Campo Experimental da Fundação Educacional de Penápolis - FUNEPE, com o objetivo de avaliar a composição bromatológica da silagem de capim elefante (Pennisetum purpureum Schum) cv. BRS Capiaçu, utilizando aditivo ou inoculantes no processo de ensilagem. Utilizou-se o delineamento inteiramente casualizado com cinco tratamentos (T) e três repetições: T1 - capim-elefante in natura; T2 - ensilado sem o uso de aditivo ou inoculante; T3 - ensilado com 7% de farelo de milho; T4 - ensilado com 10% de polpa cítrica e T5 ­ ensilado com inoculante bacteriano 20mL/kg¹ de matéria seca (MS). As amostras ensiladas foram acondicionadas em sacos plásticos com densidade de 20 kg de matéria natural (MN) por embalagem e as análises foram feitas a partir do material obtido de plantas com 110 dias de idade. Para os materiais ensilados obedeceu-se ao tempo de 30 dias para realização das análises bromatológicas onde foram avaliados os seguintes parâmetros: matéria seca (MS), nutrientes digestíveis totais (NDT) e Proteína Bruta (PB). O capim BRS Capiaçu ensilado com 7% na matéria natural de farelo de milho apresenta maiores teores de matéria seca e proteína bruta.

The experiment was carried out in the city of Penápolis/SP, in the Experimental Field of the Educational Foundation of Penápolis - FUNEPE, with the objective of evaluating the bromatological composition of elephant grass silage (Pennisetum purpureum Schum) cv. BRS Capiaçu, using additive or inoculants in the silage process. A completely randomized design with five treatments (T) and three replications was used: T1 - elephant grass without fresh additive; T2 - ensiled without the use of additives; T3 - ensiled with 7% com bran; T4 - ensiled with 10% citrus pulp and T5 - ensiled with bacterial inoculant 20 mL/kg¹ of dry matter (DM). The ensiled samples were packed in plastic bags with a density of 20 kg of natural material (MN) per package and the analyzes were made from material obtained from 110-day-old plants. For the ensiled materials, the time of 30 days for the bromatological analysis was observed, where the following parameters were evaluated: dry matter (DM), total digestible nutrients (NDT) and Crude Protein (PB). The BRS Capiaçu grass ensiled with 7% in the natural matter of corn bran has higher contents of dry matter and protein.

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O objetivo deste trabalho foi avaliar a influência das diferentes idades de corte sobre o teor de matéria seca e proteína bruta do capim BRS Capiaçu. O experimento iniciou-se com a seleção e corte das plantas matrizes para o maior aproveitamento do colmo, fez necessário eliminar as folhas superiores, pois as mesmas não brotam, e a retirada facilitou na distribuição do colmo na hora do plantio. O delineamento experimental foi realizado em blocos casualizados, totalizando quatro repetições. A unidade experimental constituiu-se de três linhas de plantio de 48,0 m de comprimento com espaçamento entre linhas de 1,5 m, totalizando 144 m². O experimento teve duração de 110 dias, sendo distribuído em três períodos: 60, 90 e 110 dias, somando assim, três idades de corte. Para a implantação da cultivar, foi realizado o preparo convencional do solo, uso do adubo para plantio na formulação NPK 4-30-10, na cobertura o fertilizante 20-05-20 conforme a análise química do solo e o plantio por colmos no sulco. Nas três idades de corte, as amostras coletadas foram acondicionadas em sacos plásticos identificados conforme o tratamento e repetição, posteriormente foram encaminhados para o laboratório. Os maiores valores de matéria seca foram obtidos com 90 e 110 dias de corte (p<0,05). Na variável proteína bruta, o maior valor foi observado aos 60 dias, porém, não diferindo do corte realizado aos 110 dias de idade (p>0.05). O corte realizado aos 110 dias favoreceu o aumento nos teores de matéria seca sem comprometer os teores de proteína bruta.

The objective of this work was to evaluate the influence of different cutting ages on the dry matter and crude protein content of BRS Capiaçu grass. The experiment began with the selection and cutting of the mother plants for the best use of the stem, it was necessary to eliminate the upper leaves, as they do not sprout, and the removal facilitated the distribution of the stem at the time of planting. The experimental design was carried out in randomized blocks, totaling four replications. The experimental unit consisted of three planting lines of 48.0 m in length with a spacing between rows of 1.5 m, totaling 144 m². The experiment lasted 110 days, being distributed in three periods: 60, 90 and 110 days, thus adding three cutting ages. For the implantation of the cultivar, the conventional soil preparation was carried out, the use of fertilizer for planting in the NPK 4-30-10 formulation, in the coverage the fertilizer 20-05-20 according to the chemical analysis of the soil and the planting by stalks in the furrow. At the three cutting ages, the samples collected were placed in plastic bags identified according to the treatment and repetition, and were later sent to the laboratory. The highest values of dry matter were obtained with 90 and 110 days of cutting (p<0.05). In the crude protein variable, the highest value was observed at 60 days of age, however, it did not differ from the cut performed at 110 days of age (p>0.05). The cut performed at 110 days favored an increase in dry matter contents without compromising crude protein contents.

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The objective of this study was to estimate sugar content variation (°Brix) in 95 accessions from the elephant grass germplasm active bank of Embrapa Dairy Cattle research center, located in Coronel Pacheco, MG, Brazil. The accessions with the highest sugar content were identified, and the effect of plant age on sugar concentration was evaluated. The experiment was conducted in randomized blocks design with two replications. Sugar content analysis occurred twice during the growth-cycle (at 70 and 100 days) along two points of the stem, one 30 cm from the base and the other at the midpoint. The analysis of variance considered the split plot model, with accessions as main plots and plant age as subplots. The means were compared using the Scott Knott test. While there was sugar content variation between accessions, increased plant age had no significant effect on the sugar content. However, some accessions did have a significant increase or decrease in sugar content as they aged. The highest and lowest mean sugar content was 6.96% (in the accession BAG80) and 4.03% (in the accession BAG13) °Brix, respectively.

O objetivo deste estudo foi estimar a variação do teor de açúcar (°Brix) existente entre 95 acessos do banco ativo de germoplasma de capim-elefante da Embrapa Gado de Leite, localizado no campo experimental de Coronel Pacheco. Foram identificados os acessos com maior teor de açúcar, bem como avaliado o efeito da idade da planta sobre a variação da concentração de açúcar. O experimento foi conduzido em blocos ao acaso com duas repetições, 95 genótipos, duas idades de planta, 70 e 100 dias de crescimento, e análise do teor de açúcar em duas secções amostradas à 30 cm da base e na metade do colmo. A análise de variância considerou modelo de parcela subdividida no tempo, sendo os acessos, as parcelas e a idade da planta as subparcelas. As médias foram comparadas pelo teste de Scott Knott. Observou-se variação no teor de açúcar entre os acessos, porém não foi observado efeito significativo no teor de açúcar devido ao aumento da idade da planta. Entretanto, alguns acessos apresentaram interação significativa com aumento ou redução do teor de açúcar em decorrência do aumento da idade da planta. O acesso de maior média de teor de açúcares entre as duas idades foi BAG80, com 6,96%, e o de menor foi BAG13, com 4,03%.

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This study examines the effect of adding coffee husks (CH), cacao by-product (CBP) and passion fruit by-product (PBP) (fresh-matter basis) in the silage of elephant grass cv. Napier on nutritional characteristics. The experiment was laid out in a completely randomized design with a 3 × 4 factorial arrangement represented by three additives (CH, CBP or PBP) and four inclusion levels (0, 12, 24 or 36%). Four replicates were used per treatment. The material was ensiled in experimental mini-silos that were opened 60 days later for chemical analysis of the produced silages. The dry matter content of the silages with CH and PBP included at levels greater than 25% was adequate. The silage with PBP inclusion showed the highest crude protein levels and the best results for in vitro dry matter digestibility. In the silage containing CH, the neutral detergent fiber content decreased linearly with increasing inclusion of the husks. The silage with CBP showed the lowest dry matter and crude protein levels and the highest pH. In conclusion, the inclusion of up to 36% PBP in the ensiling of elephant grass is recommended, as the resulting material has potential for use in ruminant feeding at times of forage scarcity.(AU)

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El objetivo de este trabajo fue evaluar modelos matemáticos comparando los parámetros cinéticos en la producción de gas in vitro en diferentes genotipos de pasto elefante. El diseño fue completamente al azar con tres repeticiones con cinco tratamientos que fueron los genotipos de pasto elefante (BRS Canará, Napier, Vruckwona, Camerún y CNPGL 93-41-1) con una madurez de rebrote a los 45 días. El inóculo ruminal se obtuvo de dos novillos Nelores machos mantenidos en potreros formados por Urochloa brizantha cv. Marandu, en el período seco del año. Las incubaciones fueron consecutivas midiendo las lecturas de presión de producción de gas realizadas en 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 y 96 horas. La determinación de la cinética de producción de gas se probó en dos modelos no lineales, que son: Monomolecular y Logístico. Donde se estimaron el volumen final (V_f / mL), la tasa de producción de gas promedio (k / h-1), el tiempo de latencia de producción (L / h) y el tiempo de digestión promedio (TMD / h-1). Los modelos probados, el Logístico con latencia, presentaron el mejor ajuste para todos los perfiles de cultivares. El cultivar BRS Canará y CNPGL 93-41-1 y Wrockwona, presentan estimaciones de los parámetros cinéticos de producción de gas similares y mejores a los de los cultivares Camerún y Napier.(AU)

The aim of this study was to evaluate mathematical models comparing the kinetic parameters in the production of in vitro gas in different genotypes of elephant grass The design was entirely randomized with three repetitions with five treatments that were the elephant grass genotypes (BRS Canará, Napier, Vruckwona, Cameroon and CNPGL 93-41-1) with age of re-growth at 45 days. The ruminal inoculum was obtained from two whole male Nelores oxen kept in pickets formed by Urochloa brizantha cv. Marandu in the dry period of the year. The incubations were consecutive, measuring the gas production pressure readings in 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 and 96 hours. The determination of gas production kinetics was tested in two non-linear models: Monomolecular and Logistic. Where the final volume (Vf/mL), average gas production rate (k/h-1), production lage time (Lag/h) and average digestion time (MDT/h) were estimated. The models tested, the Logistic with latency showed better fit for all crop profiles. The BRS Canará and CNPGL 93-41-1 and Wrockwona cultivars present estimates of kinetic parameters of gas production similar and better than those of Cameroon and Napier cultivars.(AU)

Objetivou-se neste trabalho avaliar modelos matemáticos comparando os parâmetros cinéticos na produção de gás in vitro em diferentes genótipos de capim-elefante. O delineamento foi inteiramente casualizados com três repetições com cincos tratamentos que foram os genótipos de capim-elefante (BRS Canará, Napier, Vruckwona, Cameroon e CNPGL 93-41-1) com idade de maturidade de rebrota aos 45 dias. O inóculo ruminal foi obtido através de dois bois Nelores machos inteiros mantidos em piquetes formados por Urochloa brizantha cv. Marandu, no período seco do ano. As incubações foram consecutivas mensurando as leituras de pressão de produção de gás realizadas em 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 e 96 horas. A determinação da cinética de produção de gases foi testada dois modelos não lineares que são: Monomolecular e o Logístico. Onde foram estimados o volume final ( /mL), taxa média de produção de gás ( /h-1), o tempo de latência de produção ( /h) e o tempo médio de digestão (TMD/h-1). Os modelos testados, o Logístico com latência apresentou melhor ajuste para todos os perfis das cultivares. A cultivar BRS Canará e CNPGL 93-41-1 e Wrockwona, apresentam estimativas dos parâmetros cinéticos de produção de gás semelhantes e melhores aos comparados das cultivares Cameroon e Napier.(AU)

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Tall- and dwarf-sized elephant grass cultivars have been developed for cut-and-carry system. Dwarf clones have better digestibility; on the other hand, tall-sized cultivars are more productive. The aim was to verify which grass would be most recommended for cut-and-carry: tall-sized (Elephant B and IRI-381) or dwarf (Taiwan A-146 2.37 and Mott) elephant grass cultivars to feed 24 male sheep, aged between 4 and 5 months, uncastrated, weighing approximately 24.08 ± 1.76 kg body weight which were sampled on intake, digestibility, performance, ingestive behavior, nitrogen balance, microbial protein synthesis, metabolic parameters, and ruminal degradability. This research was divided into two experiments: experiment 1 lasted 38 days, seven for adaptation and 31 for data collection. Elephant grass cultivars were supplied with a mineral mixture. Data collected were intake, digestibility, ingestive behavior, metabolic parameters, microbial protein synthesis, and performance submitted to a completely randomized design. For experiment 2, three rumen fistulae animals were sampled, lasting 20 days. In this case, a randomized block in split-plot design was applied. Both designs were with P < 0.05 and analyzed through SAS statistical software. Mott and Taiwan A-146 2.37 cultivars provided greater intake, digestibility, weight gain, feeding time, nitrogen retention, production and efficiency of microbial protein synthesis, dry matter (DM) and neutral detergent fiber (NDF) degradability, and DM, crude protein, and NDF, but shorter rumination time rather than Elephant B and IRI-381. There was also a significant difference for glucose, triglycerides, plasma urea, total serum protein, urinary urea (mg/L), and urea excretion in urine (mg/day). Dwarf elephant grass cultivars as Mott and Taiwan A-146 2.37 have greater nutritional value than tall-sized Elephant B and IRI-381. Dwarf elephant grass is recommended for cut-and-carry system.

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RESUMO

El objetivo de este trabajo fue evaluar modelos matemáticos comparando los parámetros cinéticos en la producción de gas in vitro en diferentes genotipos de pasto elefante. El diseño fue completamente al azar con tres repeticiones con cinco tratamientos que fueron los genotipos de pasto elefante (BRS Canará, Napier, Vruckwona, Camerún y CNPGL 93-41-1) con una madurez de rebrote a los 45 días. El inóculo ruminal se obtuvo de dos novillos Nelores machos mantenidos en potreros formados por Urochloa brizantha cv. Marandu, en el período seco del año. Las incubaciones fueron consecutivas midiendo las lecturas de presión de producción de gas realizadas en 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 y 96 horas. La determinación de la cinética de producción de gas se probó en dos modelos no lineales, que son: Monomolecular y Logístico. Donde se estimaron el volumen final (V_f / mL), la tasa de producción de gas promedio (k / h-1), el tiempo de latencia de producción (L / h) y el tiempo de digestión promedio (TMD / h-1). Los modelos probados, el Logístico con latencia, presentaron el mejor ajuste para todos los perfiles de cultivares. El cultivar BRS Canará y CNPGL 93-41-1 y Wrockwona, presentan estimaciones de los parámetros cinéticos de producción de gas similares y mejores a los de los cultivares Camerún y Napier.

The aim of this study was to evaluate mathematical models comparing the kinetic parameters in the production of in vitro gas in different genotypes of elephant grass The design was entirely randomized with three repetitions with five treatments that were the elephant grass genotypes (BRS Canará, Napier, Vruckwona, Cameroon and CNPGL 93-41-1) with age of re-growth at 45 days. The ruminal inoculum was obtained from two whole male Nelores oxen kept in pickets formed by Urochloa brizantha cv. Marandu in the dry period of the year. The incubations were consecutive, measuring the gas production pressure readings in 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 and 96 hours. The determination of gas production kinetics was tested in two non-linear models: Monomolecular and Logistic. Where the final volume (Vf/mL), average gas production rate (k/h-1), production lage time (Lag/h) and average digestion time (MDT/h) were estimated. The models tested, the Logistic with latency showed better fit for all crop profiles. The BRS Canará and CNPGL 93-41-1 and Wrockwona cultivars present estimates of kinetic parameters of gas production similar and better than those of Cameroon and Napier cultivars.

Objetivou-se neste trabalho avaliar modelos matemáticos comparando os parâmetros cinéticos na produção de gás in vitro em diferentes genótipos de capim-elefante. O delineamento foi inteiramente casualizados com três repetições com cincos tratamentos que foram os genótipos de capim-elefante (BRS Canará, Napier, Vruckwona, Cameroon e CNPGL 93-41-1) com idade de maturidade de rebrota aos 45 dias. O inóculo ruminal foi obtido através de dois bois Nelores machos inteiros mantidos em piquetes formados por Urochloa brizantha cv. Marandu, no período seco do ano. As incubações foram consecutivas mensurando as leituras de pressão de produção de gás realizadas em 2; 4; 6; 8; 10; 12; 19; 24; 36; 48; 60; 72; 84 e 96 horas. A determinação da cinética de produção de gases foi testada dois modelos não lineares que são: Monomolecular e o Logístico. Onde foram estimados o volume final ( /mL), taxa média de produção de gás ( /h-1), o tempo de latência de produção ( /h) e o tempo médio de digestão (TMD/h-1). Os modelos testados, o Logístico com latência apresentou melhor ajuste para todos os perfis das cultivares. A cultivar BRS Canará e CNPGL 93-41-1 e Wrockwona, apresentam estimativas dos parâmetros cinéticos de produção de gás semelhantes e melhores aos comparados das cultivares Cameroon e Napier.

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This study aimed to evaluate the chemical composition and fatty acid (FA) profile of chopped forage and silage of BRS Capiaçu elephant grass at four regrowth ages: 50, 70, 90 and 110 days. A randomized block design with five replications was used. The ensiling was carried out manually in experimental silos without wilting using no additives or bacterial inoculants. The results were analyzed using mixed models (P < 0.05). The model included treatment (regrowth age) as a fixed effect and block as a random effect. Linear and quadratic effects of the treatments were analyzed using orthogonal contrasts. There were linear increases in the dry matter (DM, g kg-¹) and lignin (g kg-¹ DM) contents and linear reductions in the in vitro DM digestibility (g kg-¹) of chopped grass and silage as a function of regrowth age (P < 0.001). Quadratic effects (P ≤ 0.01) were observed for the chopped grass contents (g kg-1 DM) of crude protein (CP), ether extract (EE) and neutral detergent fiber (NDF) as a function of regrowth age. There were linear decreases (P < 0.0001) in the CP content (g kg-¹ DM) and pH and linear increases (P < 0.001) in the EE and NDF contents (g kg-¹ DM) in the silage as a function of regrowth age. There were linear decreases (P < 0.01) in the chopped grass contents and linear increases (P < 0.05) in the silage contents of total FAs, linoleic and α-linolenic acids (g kg-¹ DM) as a function of regrowth age. BRS Capiaçu elephant grass must be harvested at up to 70 days of regrowth to obtain forage with good nutritional value and the highest levels of linoleic and α-linolenic acids (g kg-¹ DM). To produce silages with adequate pH values and the highest levels of linoleic and α-linolenic acids (g kg-¹ DM), BRS Capiaçu must be harvested between 90 and 110 days of regrowth.(AU)

O estudo objetivou avaliar a composição química e o perfil de ácidos graxos (AG) da forragem e da silagem do capim-elefante BRS Capiaçu em quatro idades de rebrota (50, 70, 90 e 110 dias), sob delineamento em blocos casualizados, com cinco repetições. A ensilagem foi realizada manualmente em silos experimentais, sem pré-murchamento, aditivos ou inoculantes bacterianos. Os resultados foram analisados usando modelos mistos (P < 0,05), que incluíram o efeito fixo de tratamento (idade de rebrota) e efeito aleatório de bloco. Os efeitos lineares e quadráticos foram analisados por meio de contrastes ortogonais. Houve aumento linear nos teores de matéria seca MS (g kg-¹) e lignina (g kg-¹ de MS), e reduções lineares na digestibilidade in vitro da MS (g kg-1) da forragem e da silagem em função da idade de rebrota (P < 0,001). Efeitos quadráticos (P ≤ 0,01) foram observados na forragem para os teores (g kg-¹ de MS) de proteína bruta (PB), extrato etéreo (EE) e fibra em detergente neutro (FDN) em função da idade de rebrota. Houve reduções lineares (P < 0,0001) no teor de PB (g kg-¹ de MS) e no pH, e aumentos lineares (P < 0,001) nos teores de EE e FDN (g kg-¹ de MS) da silagem em função de idade de rebrota. Para os teores dos AG totais e dos ácidos linoleico e α-linolênico (g kg-¹ de MS), houve reduções lineares (P < 0,01) na forragem e aumentos lineares (P < 0,05) na silagem em função da idade de rebrota. O BRS Capiaçu deve ser colhido até 70 dias de rebrota para obter forragem com bom valor nutricional e maiores teores dos ácidos linoleico e α-linolênico (g kg-¹ de MS). Para silagem com adequado pH e maiores teores dos ácidos linoleico e α-linolênico (g kg-¹ de MS), o BRS Capiaçu deve ser colhido entre 90 e 110 dias de rebrota.(AU)

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RESUMO

Objetivou-se avaliar as características químicas e nutricionais do feno de capim elefante amonizado com diferentes níveis de ureia (0,0; 0,5; 1,0; 1,5 e 2,0% da matéria seca). O delineamento experimental foi o inteiramente casualizado e a estimativa dos coeficientes de regressão foi feita através do teste T de Student (5% de significância). Os dados também foram submetidos à análise multivariada, utilizando a distância de Gower e o método UPGMA de agrupamento, além disso, foram submetidos à análise de componentes principais. O aumento das doses de ureia não alterou os teores de matéria seca, hemicelulose e material mineral do feno de capim elefante. Houve redução das frações fibrosas com o aumento das doses de ureia, contudo, houve aumento linear dos teores de digestibilidade da matéria seca (DMS), proteína bruta, nutrientes digestíveis totais (NDT) e nitrogênio amoniacal. Observou-se, através da análise multivariada, que os carboidratos não fibrosos, NDT e DMS, foram as variáveis que mais contribuíram com os resultados observados para o tratamento com 2,0% de ureia. Através da análise de Cluster, foi possível visualizar a formação de dois grupos, sendo o primeiro formado pela utilização de2,0% de ureia, que se destacou na composição bromatológica do feno de capim elefante, e o segundo grupo pelas demais doses de ureia, divididos em dois subgrupos. Em conclusão, entre as doses estudadas, recomenda-se a adição de 2,0% de ureia ao capim elefante desidratado, pois altera beneficamente a composição nutricional do feno de capim elefante, principalmente os componentes da fração fibrosa.(AU)

The objective of this study was to evaluate the chemical and nutritional characteristics of ammonized elephant grass hay with different levels of urea (0.0, 0.5, 1.0, 1.5 e 2.0% of dry matter). The experimental design was completely randomized and the regression coefficients were estimated using the Student's T test (5% significance).The data were also submitted to multivariate analysis using the Gower distance and the UPGMA method of grouping, in addition, they were subjected to principal component analysis. There was a reduction in fibrous fractions with an increase in urea doses, however, there was a linear increase in the contents of digestibility of drymatter (DDM), crude protein, total digestible nutrients (TDN) and ammoniacal nitrogen. It was observed through multivariate analysis that non-fibrous carbohydrates, TDN and DDM were the variables that most contributed to the results observed for the treatment with 2.0% urea. Through the Cluster analysis it was possible to visualize the formation of two groups, the first formed by the use of 2.0% urea, which stood out in the bromatological composition of elephant grass hay and the second group by the other doses of urea, divided into two subgroups. Thus, it is possible to conclude that among the studied doses, the addition of 2.0% urea to the dehydrated elephant grass is recommended, as it beneficially alters the nutritional composition of elephant grass hay, mainly the components of the fibrous fraction.(AU)

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RESUMO

Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.(AU)

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RESUMO

This study aimed to evaluate the chemical composition and fatty acid (FA) profile of chopped forage and silage of BRS Capiaçu elephant grass at four regrowth ages: 50, 70, 90 and 110 days. A randomized block design with five replications was used. The ensiling was carried out manually in experimental silos without wilting using no additives or bacterial inoculants. The results were analyzed using mixed models (P < 0.05). The model included treatment (regrowth age) as a fixed effect and block as a random effect. Linear and quadratic effects of the treatments were analyzed using orthogonal contrasts. There were linear increases in the dry matter (DM, g kg-¹) and lignin (g kg-¹ DM) contents and linear reductions in the in vitro DM digestibility (g kg-¹) of chopped grass and silage as a function of regrowth age (P < 0.001). Quadratic effects (P ≤ 0.01) were observed for the chopped grass contents (g kg-1 DM) of crude protein (CP), ether extract (EE) and neutral detergent fiber (NDF) as a function of regrowth age. There were linear decreases (P < 0.0001) in the CP content (g kg-¹ DM) and pH and linear increases (P < 0.001) in the EE and NDF contents (g kg-¹ DM) in the silage as a function of regrowth age. There were linear decreases (P < 0.01) in the chopped grass contents and linear increases (P < 0.05) in the silage contents of total FAs, linoleic and α-linolenic acids (g kg-¹ DM) as a function of regrowth age. BRS Capiaçu elephant grass must be harvested at up to 70 days of regrowth to obtain forage with good nutritional value and the highest levels of linoleic and α-linolenic acids (g kg-¹ DM). To produce silages with adequate pH values and the highest levels of linoleic and α-linolenic acids (g kg-¹ DM), BRS Capiaçu must be harvested between 90 and 110 days of regrowth.

O estudo objetivou avaliar a composição química e o perfil de ácidos graxos (AG) da forragem e da silagem do capim-elefante BRS Capiaçu em quatro idades de rebrota (50, 70, 90 e 110 dias), sob delineamento em blocos casualizados, com cinco repetições. A ensilagem foi realizada manualmente em silos experimentais, sem pré-murchamento, aditivos ou inoculantes bacterianos. Os resultados foram analisados usando modelos mistos (P < 0,05), que incluíram o efeito fixo de tratamento (idade de rebrota) e efeito aleatório de bloco. Os efeitos lineares e quadráticos foram analisados por meio de contrastes ortogonais. Houve aumento linear nos teores de matéria seca – MS (g kg-¹) e lignina (g kg-¹ de MS), e reduções lineares na digestibilidade in vitro da MS (g kg-1) da forragem e da silagem em função da idade de rebrota (P < 0,001). Efeitos quadráticos (P ≤ 0,01) foram observados na forragem para os teores (g kg-¹ de MS) de proteína bruta (PB), extrato etéreo (EE) e fibra em detergente neutro (FDN) em função da idade de rebrota. Houve reduções lineares (P < 0,0001) no teor de PB (g kg-¹ de MS) e no pH, e aumentos lineares (P < 0,001) nos teores de EE e FDN (g kg-¹ de MS) da silagem em função de idade de rebrota. Para os teores dos AG totais e dos ácidos linoleico e α-linolênico (g kg-¹ de MS), houve reduções lineares (P < 0,01) na forragem e aumentos lineares (P < 0,05) na silagem em função da idade de rebrota. O BRS Capiaçu deve ser colhido até 70 dias de rebrota para obter forragem com bom valor nutricional e maiores teores dos ácidos linoleico e α-linolênico (g kg-¹ de MS). Para silagem com adequado pH e maiores teores dos ácidos linoleico e α-linolênico (g kg-¹ de MS), o BRS Capiaçu deve ser colhido entre 90 e 110 dias de rebrota.

Assuntos

RESUMO

Objetivou-se avaliar as características químicas e nutricionais do feno de capim elefante amonizado com diferentes níveis de ureia (0,0; 0,5; 1,0; 1,5 e 2,0% da matéria seca). O delineamento experimental foi o inteiramente casualizado e a estimativa dos coeficientes de regressão foi feita através do teste T de Student (5% de significância). Os dados também foram submetidos à análise multivariada, utilizando a distância de Gower e o método UPGMA de agrupamento, além disso, foram submetidos à análise de componentes principais. O aumento das doses de ureia não alterou os teores de matéria seca, hemicelulose e material mineral do feno de capim elefante. Houve redução das frações fibrosas com o aumento das doses de ureia, contudo, houve aumento linear dos teores de digestibilidade da matéria seca (DMS), proteína bruta, nutrientes digestíveis totais (NDT) e nitrogênio amoniacal. Observou-se, através da análise multivariada, que os carboidratos não fibrosos, NDT e DMS, foram as variáveis que mais contribuíram com os resultados observados para o tratamento com 2,0% de ureia. Através da análise de Cluster, foi possível visualizar a formação de dois grupos, sendo o primeiro formado pela utilização de2,0% de ureia, que se destacou na composição bromatológica do feno de capim elefante, e o segundo grupo pelas demais doses de ureia, divididos em dois subgrupos. Em conclusão, entre as doses estudadas, recomenda-se a adição de 2,0% de ureia ao capim elefante desidratado, pois altera beneficamente a composição nutricional do feno de capim elefante, principalmente os componentes da fração fibrosa.

The objective of this study was to evaluate the chemical and nutritional characteristics of ammonized elephant grass hay with different levels of urea (0.0, 0.5, 1.0, 1.5 e 2.0% of dry matter). The experimental design was completely randomized and the regression coefficients were estimated using the Student's T test (5% significance).The data were also submitted to multivariate analysis using the Gower distance and the UPGMA method of grouping, in addition, they were subjected to principal component analysis. There was a reduction in fibrous fractions with an increase in urea doses, however, there was a linear increase in the contents of digestibility of drymatter (DDM), crude protein, total digestible nutrients (TDN) and ammoniacal nitrogen. It was observed through multivariate analysis that non-fibrous carbohydrates, TDN and DDM were the variables that most contributed to the results observed for the treatment with 2.0% urea. Through the Cluster analysis it was possible to visualize the formation of two groups, the first formed by the use of 2.0% urea, which stood out in the bromatological composition of elephant grass hay and the second group by the other doses of urea, divided into two subgroups. Thus, it is possible to conclude that among the studied doses, the addition of 2.0% urea to the dehydrated elephant grass is recommended, as it beneficially alters the nutritional composition of elephant grass hay, mainly the components of the fibrous fraction.

Assuntos

RESUMO

Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.

Assuntos

RESUMO

The objective of this study was to evaluate the effects of wilting times and application of an enzymatic-bacterial inoculant on the fermentative profile and nutritional characteristics of BRS capiaçu grass silage in a semi-arid region. Four wilting times treatments (control, 6, 24, and 30 h), with or without the addition of an enzymatic-bacterial inoculant, were analyzed as a split-plot completely randomized design with eight replications. Parameters of the rumen degradability test were analyzed using a split-plot completely randomized block design with four replications. There was no interaction between wilting times and inoculant application on pH, ammoniacal nitrogen (NH3 -N), and aerobic stability of BRS capiaçu silage. Aerobic stability was reduced by 1.2 h for every 1-h increase in wilting time. Inoculant application reduced the pH values by 2.59% and extended the aerobic stability of the silage by 19 h. There was a significant interaction of wilting times and inoculant application on the levels of malic, succinic, lactic, and acetic acids. Inoculant application increased the contents of dry matter, ash, crude protein, insoluble neutral detergent fiber, and total carbohydrates by 3.63, 6.13, 7.73, 6.39, and 9.97% compared with non-inoculated silages, respectively. Wilting times for up to 30 h and application of enzymatic-bacterial inoculant improves the fermentative profile and chemical composition and reduces dry matter losses of silage of BRS capiaçu grass harvested at 100 days of regrowth.

Assuntos