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1.
Cell Rep ; 11(11): 1786-96, 2015 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-26074079

RESUMO

The sympathetic nervous system controls smooth muscle tone and heart rate in the cardiovascular system. Postganglionic sympathetic neurons (SNs) develop in close proximity to the dorsal aorta (DA) and innervate visceral smooth muscle targets. Here, we use the zebrafish embryo to ask whether the DA is required for SN development. We show that noradrenergic (NA) differentiation of SN precursors temporally coincides with vascular mural cell (VMC) recruitment to the DA and vascular maturation. Blocking vascular maturation inhibits VMC recruitment and blocks NA differentiation of SN precursors. Inhibition of platelet-derived growth factor receptor (PDGFR) signaling prevents VMC differentiation and also blocks NA differentiation of SN precursors. NA differentiation is normal in cloche mutants that are devoid of endothelial cells but have VMCs. Thus, PDGFR-mediated mural cell recruitment mediates neurovascular interactions between the aorta and sympathetic precursors and promotes their noradrenergic differentiation.


Assuntos
Neurônios Adrenérgicos/citologia , Células Endoteliais/citologia , Endotélio Vascular/citologia , Células-Tronco Neurais/citologia , Neurogênese , Fibras Simpáticas Pós-Ganglionares/citologia , Neurônios Adrenérgicos/metabolismo , Animais , Aorta/citologia , Aorta/embriologia , Células Endoteliais/metabolismo , Endotélio Vascular/embriologia , Células-Tronco Neurais/metabolismo , Receptores do Fator de Crescimento Derivado de Plaquetas/genética , Receptores do Fator de Crescimento Derivado de Plaquetas/metabolismo , Fibras Simpáticas Pós-Ganglionares/embriologia , Peixe-Zebra
2.
Neuroscience ; 212: 120-30, 2012 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-22521827

RESUMO

During exercise, intense brain activity orchestrates an increase in muscle tension. Additionally, there is an increase in cardiac output and ventilation to compensate the increased metabolic demand of muscle activity and to facilitate the removal of CO(2) from and the delivery of O(2) to tissues. Here we tested the hypothesis that a subset of pontomedullary and hypothalamic neurons could be activated during dynamic acute exercise. Male Wistar rats (250-350 g) were divided into an exercise group (n=12) that ran on a treadmill and a no-exercise group (n=7). Immunohistochemistry of pontomedullary and hypothalamic sections to identify activation (c-Fos expression) of cardiorespiratory areas showed that the no-exercise rats exhibited minimal Fos expression. In contrast, there was intense activation of the nucleus of the solitary tract, the ventrolateral medulla (including the presumed central chemoreceptor neurons in the retrotrapezoid/parafacial region), the lateral parabrachial nucleus, the Kölliker-Fuse region, the perifornical region, which includes the perifornical area and the lateral hypothalamus, the dorsal medial hypothalamus, and the paraventricular nucleus of the hypothalamus after running exercise. Additionally, we observed Fos immunoreactivity in catecholaminergic neurons within the ventrolateral medulla (C1 region) without Fos expression in the A2, A5 and A7 neurons. In summary, we show for the first time that after acute exercise there is an intense activation of brain areas crucial for cardiorespiratory control. Possible involvement of the central command mechanism should be considered. Our results suggest whole brain-specific mobilization to correct and compensate the homeostatic changes produced by acute exercise.


Assuntos
Neurônios Adrenérgicos/fisiologia , Vias Autônomas/fisiologia , Tronco Encefálico/fisiologia , Hipotálamo/fisiologia , Bulbo/fisiologia , Condicionamento Físico Animal/fisiologia , Ponte/fisiologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Neurônios Adrenérgicos/citologia , Animais , Vias Autônomas/citologia , Biomarcadores/metabolismo , Tronco Encefálico/citologia , Hipotálamo/citologia , Masculino , Bulbo/citologia , Ponte/citologia , Proteínas Proto-Oncogênicas c-fos/biossíntese , Proteínas Proto-Oncogênicas c-fos/genética , Ratos , Ratos Wistar
3.
Eur J Pharmacol ; 674(2-3): 415-21, 2012 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-22094068

RESUMO

Because of the few studies that emphasize the in vivo use of amphetamine and ethanol, and their consequences on autonomic neurotransmission, we decided to study the effect of these drugs on peripheral noradrenergic neurotransmission of young animals. We used contractions of the vas deferens of adolescent rats as a model for the study of pre-treatment with both agents. The 30 to 40 day old adolescent rats were pre-treated with amphetamine, at doses of 3mg/kg, or ethanol at doses of 1.2 g/kg. Both agents were also used simultaneously to investigate possible interactions. The group treated with amphetamine showed a potentiation of the vas deferens contractions evoked by noradrenaline and barium (about 20%), as well as time-response contractions of calcium (about 20%). However, the response to electrical field stimulation (EFS) was not significantly changed, but the content of noradrenaline was reduced by about 50%. The group treated with ethanol showed a decrease in vas deferens contractility to noradrenaline, phenylephrine, and barium, by less than 20%. In this group, contraction by EFS was reduced by about 40% (Tonic, 2 Hz) and 20% (Phasic, 5 Hz), but the response to calcium was not changed. As after amphetamine, the content of noradrenaline was reduced by about 50%. In the group treated with amphetamine+ethanol all the changes described after the single treatments with amphetamine or ethanol were neutralized. It is concluded that a functional antagonism was shown between amphetamine and ethanol when administered simultaneously on peripheral sympathetic neurotransmission in vas deferens of adolescent animals.


Assuntos
Neurônios Adrenérgicos/citologia , Neurônios Adrenérgicos/efeitos dos fármacos , Anfetamina/farmacologia , Etanol/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Ducto Deferente/citologia , Neurônios Adrenérgicos/metabolismo , Animais , Cálcio/metabolismo , Relação Dose-Resposta a Droga , Estimulação Elétrica , Masculino , Músculo Liso/inervação , Norepinefrina/metabolismo , Ratos , Ratos Wistar , Sistema Nervoso Simpático/efeitos dos fármacos , Sistema Nervoso Simpático/fisiologia , Fatores de Tempo , Ducto Deferente/efeitos dos fármacos , Ducto Deferente/metabolismo , Ducto Deferente/fisiologia
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