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Theriogenology ; 78(1): 165-71, 2012 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-22494675

RESUMO

The aim of this study was to evaluate sperm fertilization rates and in vitro embryo development rates for sexed and non-sexed semen selected using a silane-coated silica colloid method (Isolate) or Percoll. Frozen/thawed, sexed and unsexed semen samples from four Holstein bulls were randomly allocated to one of two different density gradient selection methods. Sperm quality (motility, concentration, morphology and membrane integrity) were evaluated and compared before and after sperm selection. Sperm motility and morphology improved (P < 0.005) after the sperm selection process with no differences between the two methods. For non-sexed semen, Percoll gradient increased the mean (± SEM) percentage of sperm recovered (57.3 ± 2.8) compared to Isolate (46.0 ± 1.8; P < 0.01). However, membrane integrity was higher after Isolate than Percoll (sexed semen: 41.0 ± 0.6 vs. 38.8 ± 0.8 and non-sexed semen 60.8 ± 1.6 vs. 58.8 ± 0.5; P < 0.05). The percentage of blastocysts produced was higher when either sexed or non-sexed semen was selected by Isolate (14.0 ± 1.0; 22.0 ± 1.1) than by Percoll (10.5 ± 1.5; 17.0 ± 2.1, respectively; P < 0.05). In summary, Isolate was a more effective method for the recovery of high quality sperm for in vitro fertilization embryo production.


Assuntos
Clonagem de Organismos/métodos , Fertilização in vitro/estatística & dados numéricos , Povidona/farmacologia , Sêmen/citologia , Pré-Seleção do Sexo/métodos , Silanos/farmacologia , Dióxido de Silício/farmacologia , Animais , Separação Celular/métodos , Clonagem de Organismos/estatística & dados numéricos , Clonagem de Organismos/veterinária , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Coloides/química , Embrião de Mamíferos , Feminino , Fertilização/fisiologia , Fertilização in vitro/veterinária , Masculino , Povidona/química , Distribuição Aleatória , Pré-Seleção do Sexo/veterinária , Silanos/química , Dióxido de Silício/química
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