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Fertilization rates and in vitro embryo production using sexed or non-sexed semen selected with a silane-coated silica colloid or Percoll.
Rodríguez Villamil, P; Wei, H; Moreira, G; Caccia, M; Fernandez Taranco, M; Bó, G A.
Afiliação
  • Rodríguez Villamil P; Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina.
Theriogenology ; 78(1): 165-71, 2012 Jul 01.
Article em En | MEDLINE | ID: mdl-22494675
The aim of this study was to evaluate sperm fertilization rates and in vitro embryo development rates for sexed and non-sexed semen selected using a silane-coated silica colloid method (Isolate) or Percoll. Frozen/thawed, sexed and unsexed semen samples from four Holstein bulls were randomly allocated to one of two different density gradient selection methods. Sperm quality (motility, concentration, morphology and membrane integrity) were evaluated and compared before and after sperm selection. Sperm motility and morphology improved (P < 0.005) after the sperm selection process with no differences between the two methods. For non-sexed semen, Percoll gradient increased the mean (± SEM) percentage of sperm recovered (57.3 ± 2.8) compared to Isolate (46.0 ± 1.8; P < 0.01). However, membrane integrity was higher after Isolate than Percoll (sexed semen: 41.0 ± 0.6 vs. 38.8 ± 0.8 and non-sexed semen 60.8 ± 1.6 vs. 58.8 ± 0.5; P < 0.05). The percentage of blastocysts produced was higher when either sexed or non-sexed semen was selected by Isolate (14.0 ± 1.0; 22.0 ± 1.1) than by Percoll (10.5 ± 1.5; 17.0 ± 2.1, respectively; P < 0.05). In summary, Isolate was a more effective method for the recovery of high quality sperm for in vitro fertilization embryo production.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Pré-Seleção do Sexo / Silanos / Fertilização in vitro / Povidona / Dióxido de Silício / Clonagem de Organismos Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Pré-Seleção do Sexo / Silanos / Fertilização in vitro / Povidona / Dióxido de Silício / Clonagem de Organismos Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos