RESUMO
Overweight and obesity are typical conditions of chronic low-intensity systemic inflammatory responses, and both have become more common in recent decades, which emphasizes the necessity for healthier diet intake. Fruits such as grapes are rich in anthocyanins, one of which is delphinidin, a promising chemopreventive agent with anti-inflammatory properties. Considering that polymorphonuclear cells (PMNs) are rapidly mobilized to tissues when the inflammatory process is initiated, this study aimed to understand the impact of grape juice intake and delphinidin on the migration properties of PMNs. Overweight women ingested 500 mL of grape juice for 28 days, and then lipid and inflammatory profiles, as well as the white blood cell count (WBC), were evaluated. Additionally, the gene expression of inflammatory markers and quantified migration molecules such as CD11/CD18, ICAM-1 and VCAM-1 were evaluated in PMNs. The influence of delphinidin-3-O-glucoside in vitro on some migration properties was also evaluated. Grape juice intake did not influence the lipid profile or affect the WBC. However, NFκB gene expression was reduced in PMNs, also reducing the circulating values of IL-8, sICAM-1, and sVCAM-1. The in vitro results demonstrated that delphinidin significantly reduced the migration potential of cells and reduced CD11-/CD18-positive cells, the gene expression of ICAM-1, and the phosphorylation and gene expression of NFκB. Additionally, delphinidin also reduced the production of IL-6, IL-8, and CCL2. Grape juice, after 28 days of intervention, influenced some properties related to cell migration, and delphinidin in vitro can modify the cell migration properties.
Assuntos
Vitis , Humanos , Feminino , Vitis/metabolismo , Antocianinas/análise , Molécula 1 de Adesão Intercelular/genética , Sobrepeso , Interleucina-8 , Bebidas/análise , Movimento Celular , Glucosídeos/farmacologia , LipídeosRESUMO
Abstract The use of lactic acid bacteria (LAB) in foods as biocontrol agents against foodborne pathogens has become increasingly known. Under the premise that controlling the adhesion of microorganisms to food contact surfaces is an essential step for meeting the goals of food processing, the aim of this work was to investigate the inhibitory and anti-biofilm effectiveness of Lactobacillus rhamnosus GG (ATCC 53103) and Lactobacillus casei (ATCC 393) against Escherichia coli O157:H7, Salmonella enterica and Listeria monocytogenes. Lactobacillus strains (108UFCCFU/ml) and pathogens (104UFCCFU/ml) were evaluated to monitor LAB anti-adhesive and antibiofilm effect, in two main scenarios: (i) co-adhesion and (ii) pathogen incorporation to stainless steel surfaces with a protective biofilm of Lactobacillus cells. In (i) the predominant effect was observed in L. rhamnosus against S. enterica and L. monocytogenes, whereas in (ii) both LAB significantly reduced the number of pathogenic adherent cells. The effect of pre-established LAB biofilms was more successful in displacing the three pathogens than when they were evaluated under co-adhesion. These findings show that both LAB can be considered good candidates to prevent or inhibit the adhesion and colonization of L. monocytogenes, S. enterica and E. coli O157:H7 on surfaces and conditions of relevance for juice processing industries, offering alternatives for improving the safety and quality of fruit-based products.
Resumen Existe un creciente interés en el uso de bacterias ácido lácticas (BAL) como agentes de biocontrol frente a patógenos de transmisión alimentaria. Bajo la premisa de que el control de la adhesión de microorganismos a superficies de contacto con alimentos es el paso esencial para evitar su contaminación, el objetivo de este trabajo fue investigar la efectividad inhibitoria y antibiofilm de Lactobacillus rhamnosus GG (ATCC 53103) y Lactobacillus casei (ATCC 393) frente a Escherichia coli O157:H7, Salmonella enterica y Listeria monocytogenes. A fin de cumplir con el objetivo propuesto, las cepas de Lactobacillus (108UFCUFC/ml) y los patógenos (104UFCUFC/ml) se ensayaron en 2 escenarios: (1) coadhesión, y (2) incorporación de los patógenos a las superficies de acero inoxidable con un biofilm preformado de Lactobacillus. En (1), el efecto predominante se observó con L. rhamnosus frente a S. enterica y L. monocytogenes, mientras que en (2), ambas BAL redujeron significativamente el número de células patógenas adheridas. En función de estos resultados, concluimos que el efecto de un biofilm preformado de ambas BAL fue más exitoso en el desplazamiento de los 3 patógenos que en coadhesión. Ambas BAL pueden considerarse buenas candidatas para mitigar la adhesión y colonización de L. monocytogenes, S. enterica y E. coli O157:H7 en superficies en condiciones de relevancia para la industria procesadora de jugos, y, de esta manera, ofrecer alternativas para mejorar la seguridad y calidad de los alimentos a base de frutas.
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The effect of high hydrostatic pressure (HHP) and the proteolytic fraction P1G10 from papaya latex was studied to find out whether a synergy exists in the growth inhibition of Botrytis cinerea in grape juice, contributing to the improvement of conservation techniques and extending the shelf life and quality of food products. Grape juice (GJ) diluted to 16 °Brix with a water activity (aw) of 0.980 was prepared from a concentrated GJ and used in this study. Results indicated a 92% growth inhibition of B. cinerea when exposed to 1 mg/mL of P1G10 and 250 MPa/4 min of pressure treatment. The proximate composition and antioxidant compounds present in the GJ were not significantly affected after the treatments. Eight phenolic compounds and two flavonoids in GJ were identified and quantified, with values fluctuating between 12.77 ± 0.51 and 240.40 ± 20.9 mg/L in the control sample (0.1 MPa). The phenolic compounds showed a significant decrease after the applied treatments, with the HHP sample having a content of 65.4 ± 6.9 mg GAE/100 mL GJ. In conclusion, a synergistic effect at moderate HHP of 250 MPa/4 min with the addition of P1G10 was observed, and the successful development of a stable and acceptable GJ product was possible.
RESUMO
The use of lactic acid bacteria (LAB) in foods as biocontrol agents against foodborne pathogens has become increasingly known. Under the premise that controlling the adhesion of microorganisms to food contact surfaces is an essential step for meeting the goals of food processing, the aim of this work was to investigate the inhibitory and anti-biofilm effectiveness of Lactobacillus rhamnosus GG (ATCC 53103) and Lactobacillus casei (ATCC 393) against Escherichia coli O157:H7, Salmonella enterica and Listeria monocytogenes. Lactobacillus strains (108CFU/ml) and pathogens (104CFU/ml) were evaluated to monitor LAB anti-adhesive and antibiofilm effect, in two main scenarios: (i) co-adhesion and (ii) pathogen incorporation to stainless steel surfaces with a protective biofilm of Lactobacillus cells. In (i) the predominant effect was observed in L. rhamnosus against S. enterica and L. monocytogenes, whereas in (ii) both LAB significantly reduced the number of pathogenic adherent cells. The effect of pre-established LAB biofilms was more successful in displacing the three pathogens than when they were evaluated under co-adhesion. These findings show that both LAB can be considered good candidates to prevent or inhibit the adhesion and colonization of L. monocytogenes, S. enterica and E. coli O157:H7 on surfaces and conditions of relevance for juice processing industries, offering alternatives for improving the safety and quality of fruit-based products.
Assuntos
Escherichia coli O157 , Lactobacillales , Listeria monocytogenes , Microbiologia de Alimentos , Biofilmes , Manipulação de AlimentosRESUMO
OBJECTIVES: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the presence of aggregated amyloid-ß (Aß) peptides. Several natural compounds have been proposed against this disease and grape products are among these. However, little is known about grape juice potential. Transgenic Caenorhabditis elegans (C. elegans) strains that express human Aß have been used as an in vivo model for AD. METHODS: In this study, we have exposed CL2006 worms to nine different juices obtained from different cultivars. RESULTS: Cora, Bordo, Isabel, Isabel Precoce, BRS-Magna, BRS-Rubea and BRS-Violeta juices improved the behavioral phenotype (paralysis) that is caused by Aß aggregation in the transgenic animals at the concentrations tested and no toxic effects were found. Some juices were also able to increase the worm's lifespan. We could not attribute lifespan increase and paralysis reduction with any specific compound found in the phytochemical analysis. DISCUSSION: Our data indicate that the rich constitution of the juices is responsible for attenuating the phenotype caused by Aß aggregation in C. elegans.
Assuntos
Doença de Alzheimer , Vitis , Animais , Humanos , Peptídeos beta-Amiloides/genética , Caenorhabditis elegans , Longevidade , Animais Geneticamente Modificados , Doença de Alzheimer/genética , Paralisia , Modelos Animais de DoençasRESUMO
Over the past decade, the business of producing juice from grapes (Vitis labrusca L. and hybrids) has been expanding in response to increasing consumption encouraged by campaigns promoting the benefits grape juice can have on health. This study aimed to determine the effect of the production cycle, vine training system, and rootstock on the yield and physical and physical-chemical characteristics of 'BRS Magna' grapes grown in the Vale do Submédio São Francisco in the northeastern region of Brazil. The study was conducted in Petrolina, Pernambuco, over eight production cycles (2017 to 2020). The grapevines were trained in three vine training trellis systems: espalier, lyre, and pergola; and grafted onto two rootstocks: IAC 572 and IAC 766. The production cycle, training system, and rootstocks affected the yield components, vigor, and physical characteristics of the BRS Magna grapes. The combination of the pergola training system and rootstock IAC 766 stood out from the others, providing mean yields estimated at 26 t ha -1 per production cycle. The soluble solids content (SS), titratable acidity (TA), and SS TA -1 ratio were affected neither by the training system nor the rootstock, and higher values for SS and lower values for TA were obtained in the first, second, fourth and eighth production cycles. Thus, the pergola training system and rootstock IAC 766 are recommended for promoting yield gains in the 'BRS Magna' grape in the Vale do Submédio São Francisco.(AU)
Assuntos
Vitis/crescimento & desenvolvimento , SucosRESUMO
This study aimed to investigate the behavior of Oenococcus oeni MS9 and MS46 strains in sterile grape juice (SGJ, pH 4.0) incubated at 30 °C, in terms of growth and glucose, organic acids and total phenolic compounds utilization. In addition, their antimicrobial activity and the changes in antioxidant properties of fermented juice with selected strain were evaluated. Both strains grew without lag period by ~1.40 log CFU/mL at 12 days with maximum growth rates of about 0.019 h-1. After this time the MS9 and MS46 strains counts declined by 0.6 log units and remained unchanged respectively. O. oeni MS46 was evaluated in SGJ for low inoculum size (~104 CFU/mL). In this condition it also grew without lag period by 3.11 ± 0.01 log CFU/mL with a µmax of 0.05 h-1. Glucose and L-malic and citric acids were simultaneously utilized but at different rates and extents, yielding mainly lactic acid with concomitant pH reduction. Acetic acid ranged between 11 and 19 mmol/L. Total phenolic compounds significantly decreased in fermented SGJ with strain MS9 but not MS46. In this last condition, the antioxidant activity increased by 21%. In addition, both O. oeni strains showed antibacterial properties against Escherichia coli 700, Salmonella Typhimurium and Listeria monocytogenes. O. oeni strains, especially MS46, with the ability to growth in SGJ, high malolactic potential and adequate sugars and organic acids profiles from the sensorial viewpoint may be used to ferment grape juice with safer and healthier properties than fresh juice.
Assuntos
Fermentação , Oenococcus , Vitis , Vinho , Endopeptidases , Esterases , Glucose , Oenococcus/metabolismo , Fenóis , Vitis/microbiologia , Vinho/análise , Vinho/microbiologiaRESUMO
Iodine-131 (I-131) radioisotope it causes the formation of free radicals, which lead to the formation of cell lesions and the reduction of cell viability. Thus, the use of radioprotectors, especially those from natural sources, which reduce the effects of radiation to healthy tissues, while maintaining the sensitivity of tumor cells, stands out. The objective of the present study was to evaluate the cytoprotective/radioprotective effects of whole grape juices manufactured from the conventional or organic production systems, whether or not exposed to ultraviolet (UV-C) light irradiation. The results showed that I-131 presented a cytotoxic effect on human hepatocellular cells (HepG2/C3A) at concentrations above 1.85 MBq/mL, after 24 and 48 hours of treatment, though all concentrations (0.0037 to 7.40 MBq/mL) were cytotoxic to non-tumor human lung fibroblast (MCR-5) cells, after 48 hours. However, grape juices (10 and 20 µL/mL) did not interfere with the cytotoxic effect of the therapeutic dose of I-131 on tumor cells within 48 hours of treatment, while protecting the non-tumor cells, probably due to its high antioxidant activity. In accordance with their nutraceutical potential, antioxidant and radioprotective activity, these data stimulate in vivo studies on the use of natural products as radioprotectants, such as grape juice, in order to confirm the positive beneficial potential in living organisms.
O radioisótopo iodo-131 (I-131) causa a formação de radicais livres, que levam à formação de lesões celulares e redução da viabilidade celular. Assim, destaca-se a utilização de radioprotetores, principalmente de origem natural, que reduzem os efeitos da radiação nos tecidos saudáveis, mantendo a sensibilidade das células tumorais. O objetivo do presente estudo foi avaliar os efeitos citoprotetores/radioprotetores de sucos de uva integral fabricados em sistemas de produção convencional ou orgânico, expostos ou não à radiação ultravioleta (UV-C). Os resultados mostraram que o I-131 apresentou efeito citotóxico nas células hepatocelulares humanas (HepG2/C3A) em concentrações acima de 1,85 MBq/mL, após 24 e 48 horas de tratamento, embora todas as concentrações (0,0037 a 7,40 MBq/mL) fossem citotóxicas para células de fibroblasto de pulmão humano não tumoral (MCR-5), após 48 horas. No entanto, os sucos de uva (10 e 20 µL/mL) não interferiram no efeito citotóxico da dose terapêutica de I-131 nas células tumorais em 48 horas de tratamento, protegendo as células não tumorais, provavelmente devido ao seu alto poder antioxidante. atividade. De acordo com seu potencial nutracêutico, atividade antioxidante e radioprotetora, esses dados estimulam estudos in vivo sobre o uso de produtos naturais como radioprotetores, como o suco de uva, a fim de confirmar o potencial benéfico positivo em organismos vivos.
Assuntos
Humanos , Protetores contra Radiação , Radioterapia/efeitos adversos , Compostos Radiofarmacêuticos/toxicidade , Vitis , SucosRESUMO
Continuous fungicide spraying is required to eliminate fungal pathogens on grapes. However, this practice is associated with several risks, including contamination and environmental imbalance, as well as toxicity to operators and the induction of resistance in pathogens. In addition, a strong correlation has been reported between the presence of fungicides and the occurrence of issues during alcoholic fermentation, resulting in negative impacts on the sensory quality of the final products. Numerous studies have evaluated residue concentrations of phytosanitary products in grapes, juices, and wines, and a significant number of studies have assessed the impact of different agrochemicals on bioprocesses. However, a review compiling the key results of these studies is currently lacking. This review incorporates results obtained in the last decade from research on the presence of fungicide residues, including azoxystrobin, boscalid, captan, copper, fenhexamid, folpet, pyraclostrobin, pyrimethanil and tebuconazole, and their effects on fermentation kinetics. Practical solutions to mitigate these problems, both in vineyards and industry, are also presented and discussed. This review highlights the constant high fungicidal agent concentrations (greater than 1 or 2 mg L-1) used throughout the winemaking process, with the impact of residues being of particular concern, especially with regard to their effect on yeast activity and the fermentation process. Thus, the adoption of methodologies that allow winemakers to control and trace these residues is an important step in avoiding or reducing fermentation problems throughout the winemaking process.[Figure: see text].
Assuntos
Contaminação de Alimentos/análise , Fungicidas Industriais/análise , Vinho/análise , Fermentação , Humanos , Pirimidinas/análise , Saccharomyces cerevisiae/metabolismo , Estrobilurinas/análise , Triazóis/análiseRESUMO
Parkinson's disease (PD) is a neurodegenerative disorder with significant motor disabilities and cognitive decline. Importantly, the imbalance of oxidative stress is related to PD physiopathology and progression. This study aimed to evaluate the impact of grape juice consumption associated with an aquatic exercise protocol on oxidative stress parameters and cognitive function in individuals with PD. The participants were randomized into two groups: grape juice group (GJG) and control group (CG) and were submitted to 4 weeks of an aquatic intervention (twice a week, approximately 60 minutes/session). The GJG also consumed 400 ml of grape juice per day (integral and conventional) during this period. Cognitive function was assessed by the Montreal Cognitive Assessment (MoCa) questionnaire. For the analysis of oxidative stress markers, specifically lipid oxidative damage (TBARS), proteins (Carbonil), acid uric and the activity of antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase), blood collection were done before and after intervention. No changes were observed in cognitive function after intervention in both groups. Regarding biomarkers, a reduction of antioxidant enzymes, thiobarbituric acid reactive substances (TBARS) and uric acid was observed in both groups. However, only the GJG showed a significant reduction on protein oxidation levels after intervention. In conclusion, the consumption of grape juice associated with an aquatic exercise protocol might be consider an effective alternative to reduce the oxidative damage in PD, reinforcing the importance of this intervention in promoting beneficial impact in this population.
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Preeclampsia (PE) is a hypertensive disorder of pregnancy and it is one of the main causes of maternal and fetal morbidity and mortality worldwide. It is known that oxidative stress plays a role in its pathophysiology, therefore we investigated the effects of trans-resveratrol, a potent antioxidant, on the Nrf2/ARE pathway, nitric oxide (NO) production, and reactive oxygen species (ROS) levels in an in vitro model of PE. Plasma from PE patients increased ARE activity in endothelial cells compared with plasma from healthy pregnant (HP), and the addition of resveratrol was able to potentiate this increase only in PE. Resveratrol also decreased ROS levels in the cells incubated with plasma from PE. Based on these results, we performed a pilot clinical study to compare the effects of serum from PE women before and 1 h after ingestion of polyphenol-rich whole red grapefruit juice incubated on endothelial cells, since grapefruit contains large amounts of resveratrol. Serum from PE patients, obtained one hour after juice intake, decreased antioxidants markers in cells compared with the serum before juice intake, besides, it increased NO production. In conclusion, resveratrol and polyphenol-rich red grape juice have potentially beneficial effects on endothelial cells incubated with PE plasma/serum, which could aid in the management of PE.
Assuntos
Antioxidantes/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Óxido Nítrico/metabolismo , Pré-Eclâmpsia/sangue , Resveratrol/farmacologia , Adulto , Estudos de Casos e Controles , Feminino , Sucos de Frutas e Vegetais , Humanos , Estresse Oxidativo , Projetos Piloto , Gravidez , VitisRESUMO
This study aimed to evaluate the effects of purple grape juice consumption in pregnancy on oxidative stress parameters in Wistar rat fetuses. Twenty-four pregnant rats were divided into five groups: control group, indomethacin group (received a single dose of indomethacin in DG20), group grape juice DG14 (received an amount for 14 days/first and second gestational trim), group grape juice DG20 (received a dose throughout the gestational period), group grape juice two doses (received two doses, at morning and afternoon). On the 20th day of pregnancy (DG20), rats were anesthetized, and a cesarean section was performed to obtain the fetuses. A sample of liver, heart, and total brain of fetuses was collected for oxidative stress analyses. Values P<0.05 were considered significant. In fetuses' heart, we observed that the grape juice two dose group decreased sulfhydryl and increased SOD. In the liver, the grape juice decreased TBARS and SOD. There was a decrease in carbonyl and sulfhydryl in the indomethacin and grape juice one dose groups in the brain. We conclude that indomethacin altered oxidative stress parameters only in the fetal brain, and grape juice was presented as an important modulator of antioxidant capacity when consumed in a dose.
RESUMO
Grapes and their derivatives have antioxidant and cardioprotective properties. Therefore, we hypothesized that grape juice (GJ) could improve vascular oxidative damage caused by chlorine radicals (OCl-), which are excessively produced in vascular tissue during cardiovascular diseases (mainly diabetes and hypertension). The antioxidant capacity of GJ was analyzed by an electrochemical method, followed by administration in rats (100 or 300 mg/kg/d, via the oral) for seven days. Then, rats were sacrificed, and their aortas were isolated and subjected to isometric recordings or immuno-histochemical analyses with or without exposure to OCl- (5, 20, or 100 µM, 60 min). Concentration-effect curves for acetylcholine (ACh) and sodium nitroprusside (SNP) were derived to analyze endothelium-dependent or independent vasore-laxation. The GJ presented with high antioxidant capacity, and treatment with GJ did not alter vascular relaxation induced by ACh or SNP. After exposure to OCl-, endothelium-denuded arteries showed preserved relaxation with SNP, whereas endothelium-intact arteries showed reduced relaxation with ACh. OCl- at various concentrations induced significantly decreased relaxation of arteries (80.6±4.2%, 55.4±4.7%, and 28.1±5.9%, respectively) vs. control arteries (96.8±2.4%). However, treatment with GJ prevented loss in relaxation caused by 5 and 20 µM OCl- and improved relaxation after exposure to 100 µM OCl-. Exposure to OCl- induced increased nitrotyrosine immunostaining of endothelial cell layers, which was improved by GJ treatment. Altogether, vascular damage caused by OCl- was prevented by treatment with GJ, and GJ prevented nitrosative stress in these vessels.
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The objective was to investigate the role of consumption of grape juice (GJ), red wine (RW) or resveratrol solution (RS) on rats treated with a high-fat diet (HFD). Among the drinks offered, GJ had lower content of polyphenols and trans-resveratrol. Nevertheless, GJ showed similar content of anthocyanin and antioxidant activity to RW, although higher than RS. In rats treated with HFD, consumption of GJ presented best antioxidant and anti-inflammatory activity, reducing glutathione peroxidase and interleukin-6 serum levels. In addition, GJ promoted better levels of cholesterol and liver markers. On the other hand, RW aggravated the oxidizing effect of HFD, increasing catalase activity and interleukin-6 level. Already, RS showed no benefit in animals. Thus, GJ minimized the effects of HFD on oxidative stress and inflammation beyond promoted better levels of lipid profile and liver biomarkers. However, consumption of RS showed no benefit and RW revealed a pro-oxidant effect, as did HFD. [Figure: see text].
Assuntos
Vitis , Vinho , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Bebidas , Dieta Hiperlipídica/efeitos adversos , Lipídeos , Estresse Oxidativo , Ratos , Ratos Wistar , Resveratrol/farmacologia , Vitis/metabolismoRESUMO
Objetivou-se avaliar o comportamento fenológico, e as características físico-químicas e produtivas da videira BRS Carmem cultivadas em Palotina, PR. A avaliação das videiras enxertadas sobre IAC 572 Tropical, espaçadas em 1,5 x 2,5 m, e conduzidas em espaldeira foi realizada nas safras 2019/2020 e 2020/2021. Foram utilizadas 20 plantas representativas do pomar experimental da UFPR Setor Palotina. A avaliação do comportamento fenológico foi realizada a partir de observação de ramos selecionados, a partir da poda de frutificação realizada em meados de agosto, caracterizando-se a duração em dias de cada um dos seguintes subperíodos: poda à gema-algodão (PO-GA); poda à brotação (PO-BR); poda ao aparecimento da inflorescência (PO-AI); poda ao florescimento (PO-FL); poda ao início da maturação (PO-IM) das bagas; poda à colheita (PO-CO), sendo confeccionados diagramas em escala de dias. Por ocasião da colheita quantificou-se o número de cachos por planta, massa dos cachos e a partir destes dados foram estimadas a produção (kg planta-1) e produtividade (t ha-1). Para avaliaçãodo mosto foram coletados dois cachos por planta, dos quais retirou-se seis bagas por cachopara avaliação do teor de sólidos solúveis (SS), acidez titulável (AT), relação de índice de maturação (SS/AT) e o teor de antocianinas (mg g-1). Quanto ao número de cachos verificou se em média 48 cachos por planta com massa média de 133 g. As estimativas da produção por planta e produtividade foram de 6,4 kg e 17,1 t ha-1, respectivamente. Os teores médios de SS, AT, SS/AT e antocianinas, foram de 18°Brix, 0,97% deácido tartárico, 18,4 e 4,97 mg g-1, respectivamente. As características produtivas e a qualidade do mosto da BRS Carmem cultivadas na região Oeste do Paraná estão dentro dos padrões desejáveis para elaboração de suco de uva.(AU)
The objective of this study was to evaluate the phenological behavior and the physical-chemical and productive characteristics of the BRS Carmem vine grown in the Western region of Paranástate (Brazil). The evaluation of vines grafted on IAC 572 Tropical, spaced at 1.5 x 2.5 m, and conducted on a backrest was performed in the harvests 2019/2020 and 2020/2021. Twenty plants representing the experimental orchard of UFPR -Palotina Sector were used. The evaluation of phenological behavior was performed from observation of selected branches, from the fruiting pruning carried out in mid-August, characterizing the duration in days of each of the following subperiods: cotton yolk pruning (PO-GA); pruning to sprouting (PO-BR); pruning to the appearance of inflorescence (PO-AI); pruning to flowering(PO-FL); pruning at the beginning of ripening (PO-IM) of the berries; pruning to harvest (PO-CO), and diagrams were made on a day scale. At the harvesttime, the number of bunchesper plant, mass of bunches was quantified and from these data the production (kg plant-1) and the productivity (t ha-1) were estimated. To evaluate the must, two bunches per plant were collected, and six berries were collected per bunch to evaluate soluble solids content (SS), titratable acidity (TA), maturation index ratio (SS/TA) and anthocyanin content (mg g-1). For the number of bunches was verified an average of 48 bunches per plant, with an average mass of 133 g. The production per plantand yieldestimateswere 6.4 kg and 17.1 t ha-1, respectively. The mean levels of SS, AT, SS/AT and anthocyanins were 18°Brix, 0.97% of tartaric acid, 18.4 and 4.97 mg g-1, respectively. Therefore, theproductivecharacteristics and the quality of the 'BRS Carmem' cultivated in the Western region of Paraná state are within the desirable standards for grape juicepreparation.(AU)
Assuntos
Vitis/química , Vitis/crescimento & desenvolvimento , Fenômenos Fisiológicos Vegetais , Fenômenos QuímicosRESUMO
Objetivou-se avaliar o comportamento fenológico, e as características físico-químicas e produtivas da videira BRS Carmem cultivadas em Palotina, PR. A avaliação das videiras enxertadas sobre IAC 572 Tropical, espaçadas em 1,5 x 2,5 m, e conduzidas em espaldeira foi realizada nas safras 2019/2020 e 2020/2021. Foram utilizadas 20 plantas representativas do pomar experimental da UFPR Setor Palotina. A avaliação do comportamento fenológico foi realizada a partir de observação de ramos selecionados, a partir da poda de frutificação realizada em meados de agosto, caracterizando-se a duração em dias de cada um dos seguintes subperíodos: poda à gema-algodão (PO-GA); poda à brotação (PO-BR); poda ao aparecimento da inflorescência (PO-AI); poda ao florescimento (PO-FL); poda ao início da maturação (PO-IM) das bagas; poda à colheita (PO-CO), sendo confeccionados diagramas em escala de dias. Por ocasião da colheita quantificou-se o número de cachos por planta, massa dos cachos e a partir destes dados foram estimadas a produção (kg planta-1) e produtividade (t ha-1). Para avaliaçãodo mosto foram coletados dois cachos por planta, dos quais retirou-se seis bagas por cachopara avaliação do teor de sólidos solúveis (SS), acidez titulável (AT), relação de índice de maturação (SS/AT) e o teor de antocianinas (mg g-1). Quanto ao número de cachos verificou se em média 48 cachos por planta com massa média de 133 g. As estimativas da produção por planta e produtividade foram de 6,4 kg e 17,1 t ha-1, respectivamente. Os teores médios de SS, AT, SS/AT e antocianinas, foram de 18°Brix, 0,97% deácido tartárico, 18,4 e 4,97 mg g-1, respectivamente. As características produtivas e a qualidade do mosto da BRS Carmem cultivadas na região Oeste do Paraná estão dentro dos padrões desejáveis para elaboração de suco de uva.
The objective of this study was to evaluate the phenological behavior and the physical-chemical and productive characteristics of the BRS Carmem vine grown in the Western region of Paranástate (Brazil). The evaluation of vines grafted on IAC 572 Tropical, spaced at 1.5 x 2.5 m, and conducted on a backrest was performed in the harvests 2019/2020 and 2020/2021. Twenty plants representing the experimental orchard of UFPR -Palotina Sector were used. The evaluation of phenological behavior was performed from observation of selected branches, from the fruiting pruning carried out in mid-August, characterizing the duration in days of each of the following subperiods: cotton yolk pruning (PO-GA); pruning to sprouting (PO-BR); pruning to the appearance of inflorescence (PO-AI); pruning to flowering(PO-FL); pruning at the beginning of ripening (PO-IM) of the berries; pruning to harvest (PO-CO), and diagrams were made on a day scale. At the harvesttime, the number of bunchesper plant, mass of bunches was quantified and from these data the production (kg plant-1) and the productivity (t ha-1) were estimated. To evaluate the must, two bunches per plant were collected, and six berries were collected per bunch to evaluate soluble solids content (SS), titratable acidity (TA), maturation index ratio (SS/TA) and anthocyanin content (mg g-1). For the number of bunches was verified an average of 48 bunches per plant, with an average mass of 133 g. The production per plantand yieldestimateswere 6.4 kg and 17.1 t ha-1, respectively. The mean levels of SS, AT, SS/AT and anthocyanins were 18°Brix, 0.97% of tartaric acid, 18.4 and 4.97 mg g-1, respectively. Therefore, theproductivecharacteristics and the quality of the 'BRS Carmem' cultivated in the Western region of Paraná state are within the desirable standards for grape juicepreparation.
Assuntos
Fenômenos Fisiológicos Vegetais , Fenômenos Químicos , Vitis/crescimento & desenvolvimento , Vitis/químicaRESUMO
INTRODUCTION: Some foods are also demonstrated benefits, such as anti-inflammatory, antioxidant, and ergogenic activity, similar to that of sports supplements. Grape juice has been considered an important source of polyphenols and these compounds could promote positive effects to the sports players. In this sense, the objective was to evaluate the effects of purple grape juice consumption on indicators of oxidative stress, inflammation, muscle damage, global histone H4 acetylation levels, and muscle strength and muscle power in volleyball athletes. METHODS: This is a randomized double-blind clinical trial in which 12 male volleyball players (16 ± 0.6 years old) participated in three different moments with match simulation: control (without beverage) (WB), grape juice (GJ) and placebo (PLA) (400 mL/day of grape juice or placebo (maltodextrin) for 14 days in a cross-over model). Before and immediately after each match, blood collection for analysis of indicators of systemic redox status, systemic concentrations of Interferon-γ (IFN- γ) and Interleukin-4 (IL-4), muscle damage, by Creatine Kinase (CK-NAC) and levels of global histone H4 acetylation were performed, as well as handgrip strength (HG) and lower limb power tests. RESULTS: Consumption of grape juice significantly reduced lipid peroxidation (p = 0.04) and Deoxyribonucleic Acid (DNA) damage (p = 0.01) after the match. IFN-γ levels, IL-4, CK-NAC, and histone H4 acetylation post-match did not alter with the grape juice consumption. Lower limb power improved after acute exercise in WB and GJ conditions (p < 0.001). CONCLUSION: In this pilot trial, the intake of grape juice for two weeks seems to reduce the protein oxidation and DNA damage by intermittent physical exercise, without epigenetics influence.
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Sucos de Frutas e Vegetais , Inflamação/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Vitis , Voleibol , Adolescente , Desempenho Atlético , Creatina Quinase/efeitos dos fármacos , Método Duplo-Cego , Histonas/efeitos dos fármacos , Humanos , Masculino , Força Muscular/efeitos dos fármacosRESUMO
Preeclampsia (PE) is a multifactorial hypertensive disorder of pregnancy that is partly responsible for both maternal and fetal morbidity and mortality levels worldwide. It has been recently discovered that sirtuin-1 (SIRT1) is reduced in the circulation and in an in vitro model of PE. Therefore, in this study, we investigated the effects of trans-resveratrol, a potent antioxidant and activator of SIRT1, on oxidative stress and nitric oxide (NO) production in an in vitro model of PE compared to gestational hypertensive (GH) and healthy pregnant (HP) women. Furthermore, we also evaluated the effects of an acute intake of grape juice on women with PE to assess whether it could mimic in vitro trans-resveratrol supplementation. (1) In the GH group, resveratrol decreased intracellular reactive oxygen species (ROS) and increased their antioxidant capacity, while inhibiting SIRT1 reestablished previous levels. (2) In PE, inhibition of SIRT1 increased antioxidant activity. (3) Intracellular NO and supernatant nitrite levels were increased by inhibiting SIRT1 in the PE group. (4) Grape juice intake increased intracellular NO levels versus before grape juice intake control; however, the inhibition of SIRT1 before grape juice intake initially increased NO, but decreased it 1 h after grape juice intake. In conclusion, activating SIRT1 by using resveratrol alone may not be beneficial to women with PE, and GH and PE seem to have different responsive mechanisms to this molecule. Furthermore, grape juice intake seems to have different effects compared to resveratrol supplementation alone in this in vitro model of PE, demonstrating the potential of the combination of other biologically active molecules from grape juice over the SIRT1-eNOS-NO in PE treatment.
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Sucos de Frutas e Vegetais , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Pré-Eclâmpsia/metabolismo , Resveratrol/farmacologia , Sirtuína 1/metabolismo , Vitis , Adolescente , Adulto , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Feminino , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Projetos Piloto , Pré-Eclâmpsia/terapia , Gravidez , Resveratrol/uso terapêutico , Resultado do Tratamento , Adulto JovemRESUMO
Maternal high fat diet (HFD) and obesity during pregnancy increase female offspring's mammary cancer risk in animal studies. We aimed to observe whether the consumption of grape juice during pregnancy can reverse this risk. During pregnancy and lactation, female Wistar rats were fed either a control or HFD and also received grape juice or tap water. At the age of 50 days, female offspring were euthanized, and mammary glands were collected to assess changes in biomarkers of increased mammary cancer risk. Maternal HFD increased the number of terminal end buds in offspring's mammary glands and promoted cell proliferation (ki67). Maternal grape consumption blocked these effects. Apoptosis marker caspase 7, but not caspase 3, was reduced in the HFD offspring. HFD offspring also exhibited a reduction in the indicators of cell cycle regulation (p27, p21) and an ability to maintain DNA integrity (reduced p53). Maternal grape juice did not have any effect on these endpoints in the HFD offspring but reduced caspase 7 and p53 levels in the control offspring, perhaps reflecting reduced cellular stress. Maternal HFD increased oxidative stress marker GPx1 mRNA expression, and grape juice increased the levels of GPx2 in both the control and HFD offspring. HFD increased XBP1/Xbp1s, Atf4 and Atf6 mRNA expression and reduced ATF6 and CHOP protein levels. Maternal grape juice reversed the increase in XBP1/Xbp1s, Atf4 and Atf6 in the HFD offspring. PPAR was downregulated in the HFD group, and grape juice reversed this effect. Grape juice also reduced the levels of HER2 and IRS, both in the control and HFD offspring. In conclusion, maternal grape juice supplementation reversed some of the biomarkers that are indicative of increased breast cancer risk in the HFD offspring.
Assuntos
Sucos de Frutas e Vegetais/efeitos adversos , Glândulas Mamárias Animais/metabolismo , Fenômenos Fisiológicos da Nutrição Materna , Resposta a Proteínas não Dobradas/fisiologia , Vitis , Animais , Dieta Hiperlipídica/efeitos adversos , Ingestão de Alimentos , Feminino , Estresse Oxidativo , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Ratos , Ratos WistarRESUMO
This study aimed at evaluating the potential of the peroxidase (PO) enzyme to reduce ochratoxin A (OTA) levels and its application to grape juice. Both commercial PO and PO extracted from rice bran were evaluated, respectively, regarding their activity towards OTA in a model system. The affinity between PO and OTA was verified by the Michaelis-Menton constant and the maximum velocity parameters, resulting in 0.27 µM and 0.015 µM min-1 for the commercial enzyme, and 6.5 µM and 0.031 µM min-1 for PO extracted from rice bran, respectively. The lowest residual OTA levels occurred when 0.063 U mL-1 of the enzyme was applied. Under these conditions, the OTA reduction was 41% in 5 h for the commercial enzyme, and 59% in 24 h, for PO extracted from rice bran. When the extracted PO, with the activity of 0.063 U mL-1, was applied to whole grape juice, the OTA levels decreased to 17%, at 24 h. The capacity shown by PO for reducing OTA levels was confirmed in whole white grape juice, as a model system. This study may assist the wine industry to offer healthier products and add value to rice bran. Graphical abstract.