RESUMO
Pepper (Capsicum annuum L.) is a vegetable consumed worldwide, primarily used for vitamin C uptake and condiment purposes. Ascorbate (Asc) is a multifunctional metabolite, acting as an antioxidant and enzymatic cofactor involved in multiple cellular processes. Nevertheless, there is no evidence about the contribution of biosynthesis pathways and regulatory mechanisms responsible for Asc reserves in pepper plants. Here, we present a genome- and transcriptome-wide investigation of genes responsible for Asc biosynthesis in pepper during fruit development, stresses, and phytohormone exposures. A total of 21 genes, scattered in ten of twelve pepper chromosomes were annotated. Gene expression analyses of nine transcriptomic experiments supported the primary role of the L-galactose pathway in the Asc-biosynthesizing process, given its constitutive, ubiquitous, and high expression profile observed in all studied conditions. However, genes from alternative pathways generally exhibited low expression or were unexpressed and appeared to play some secondary role under specific stress conditions and phytohormone treatments. Taken together, our findings provide a deeper spatio-temporal understanding of expression levels of genes involved in Asc biosynthesis, and they highlight GGP2, GME1 and 2, and GalLDH members from L-galactose pathway as promising candidates for future wet experimentation, addressing the attainment of increase in ascorbate content of peppers and other crops.
RESUMO
Introdução: a Morinda citrifolia L. (noni) é uma fruta que foi recentemente introduzida no Brasil, como uma matéria-prima com forte apelo comercial devido a todas as características benéficas a ele atribuídas e os benefícios relacionados ao seu consumo. Objetivo: o presente trabalho teve como objetivo caracterizar frutos de noni em três estádios de maturação. Métodos: os frutos foram colhidos em pomares domésticos no município de Mossoró-RN, em três estádios de maturação, conforme a cor: verde (casca verde), de vez (verde esbranquiçado) e maduro (amarelo esbranquiçado). Para a caracterização do fruto, realizaram-se as seguintes análises: massa fresca, comprimentos longitudinal e transversal, DL/DT, firmeza e rendimento de polpa, vitamina C, sólidos solúveis (SS), acidez titulável (AT), pH e relação SS/AT. Adotou-se o delineamento inteiramente casualizado, com três tratamentos e doze repetições de um 1 fruto para cada parcela. Resultados: o fruto do noni é de formato ovóide, suculento e apresenta várias sementes triangulares de coloração vermelha. Considerando a polpa, ocorre mudança de coloração, passando da cor verde para a amarela esbranquiçada, à medida que o fruto amadurece. De acordo com os resultados obtidos, os estádios apresentam variabilidade para quase todos os caracteres físicos e físico-químicos. Conclusões: os frutos que apresentaram as melhores características físicas de massa e firmeza da polpa, tanto para consumo in natura, como para processamento agroindustrial são os de vez e verdes, com valores de 56,33 g e 128,41 N, respectivamente. Este fruto é considerado rica fonte de vitamina C, apresenta elevados teores de sólidos solúveis quando maduro (10,33 °Brix) e alta relação sólidos solúveis/acidez titulável em todos os estádios avaliados.
Introduction: a Morinda citrifolia L. (noni) is a fruit that was recently introduced in Brazil, as a raw material with fort I appeal advertising due to all the beneficial characteristics attributed to it and the related benefits of its consumption. Objectives: the objective of the present paper was to characterize noni fruits in three stages of maturation. Methods: the fruits had been harvested in domestic orchards in the city of Mossoró-RN, in three stages of maturation, according to the color: green (green rind), breaker stage (yellowish green) and mature (yellowish white). For the characterization of the fruit, the following analyses were performed: fresh matter, longitudinal and transversal length, DL/DT, firmness and income of pulp, acid ascorbic, soluble solids (SS), titratable acidity (AT), pH and SS/AT ratio. The experimental design was in randomized blocks, with three treatments and twelve repetitions of one (1) fruit for each parcel. Results: the noni fruit has ovoid format, is succulent and presents several triangular red seeds. Considering the pulp, coloration change occurs, going from green to yellowish white color according to the ripeness of fruits. Regarding the obtained results, the stages present variability for almost all the physical and physical chemical characters. Conclusions: the fruits that presented the best physical characteristics of mass and firmness of pulp, both for natural consumption and for agro-industrial processing are breaker stages and green fruits, with values of 56.33 g and 128.41 N, respectively. Noni is considered a rich source of vitamin C, presents high value of soluble solid when mature (10.33 Brix) and high soluble solids/titratable acidity ratio in all of the evaluated stages.
RESUMO
O objetivo deste relato é alertar a comunidade médica sobre o escorbuto que, apesar de ser doença rara nos dias atuais, deve ser lembrado quando o paciente manifesta distúrbios hemorrágicos e história de erro alimentar. Reporta-se o caso de uma criança que foi hospitalizada por dor progressiva nos membros inferiores, edema e manifestaçõeshemorrágicas. O diagnóstico de escorbuto foi baseado nos achados clínicos, no relato histórico alimentar de ingestão somente de bolo e leite e na remissão dos sinais e sintomas após a administração de vitamina C.
The purpose of this report is to alert the medical community about scurvy. Although it is nowadays a rare disease, it must be taken into consideration when the patient presents bleeding disorders and bad eating habits. A case is presented of a child hospitalized with progressive pain in his lower limbs, edema and hemorrhages. The diagnosis of scurvy was based on clinical findings, on the alimentary history of only cake and milk intake and, on the remission of symptoms and signs after the administration of vitamin C.