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Genome and Transcriptome Analyses of Genes Involved in Ascorbate Biosynthesis in Pepper Indicate Key Genes Related to Fruit Development, Stresses, and Phytohormone Exposures.
Aguiar, Évelyn Silva de; Dias, Abigailde Nascimento; Sousa, Raquel Mendes; Germano, Thais Andrade; de Sousa, Renato Oliveira; Miranda, Rafael de Souza; Costa, José Hélio; Dos Santos, Clesivan Pereira.
Afiliação
  • Aguiar ÉS; Postgraduate Program in Environmental Sciences, Center of Sciences of Chapadinha, Federal University of Maranhão, Boa Vista, Chapadinha 65500-000, Maranhão, Brazil.
  • Dias AN; Center of Sciences of Chapadinha, Federal University of Maranhão, Boa Vista, Chapadinha 65500-000, Maranhão, Brazil.
  • Sousa RM; Center of Sciences of Chapadinha, Federal University of Maranhão, Boa Vista, Chapadinha 65500-000, Maranhão, Brazil.
  • Germano TA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451-970, Ceará, Brazil.
  • de Sousa RO; Postgraduate Program in Agricultural Sciences, Campus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus 64900-000, Piauí, Brazil.
  • Miranda RS; Postgraduate Program in Agricultural Sciences, Campus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus 64900-000, Piauí, Brazil.
  • Costa JH; Plant Science Department, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.
  • Dos Santos CP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451-970, Ceará, Brazil.
Plants (Basel) ; 12(19)2023 Sep 23.
Article em En | MEDLINE | ID: mdl-37836106
Pepper (Capsicum annuum L.) is a vegetable consumed worldwide, primarily used for vitamin C uptake and condiment purposes. Ascorbate (Asc) is a multifunctional metabolite, acting as an antioxidant and enzymatic cofactor involved in multiple cellular processes. Nevertheless, there is no evidence about the contribution of biosynthesis pathways and regulatory mechanisms responsible for Asc reserves in pepper plants. Here, we present a genome- and transcriptome-wide investigation of genes responsible for Asc biosynthesis in pepper during fruit development, stresses, and phytohormone exposures. A total of 21 genes, scattered in ten of twelve pepper chromosomes were annotated. Gene expression analyses of nine transcriptomic experiments supported the primary role of the L-galactose pathway in the Asc-biosynthesizing process, given its constitutive, ubiquitous, and high expression profile observed in all studied conditions. However, genes from alternative pathways generally exhibited low expression or were unexpressed and appeared to play some secondary role under specific stress conditions and phytohormone treatments. Taken together, our findings provide a deeper spatio-temporal understanding of expression levels of genes involved in Asc biosynthesis, and they highlight GGP2, GME1 and 2, and GalLDH members from L-galactose pathway as promising candidates for future wet experimentation, addressing the attainment of increase in ascorbate content of peppers and other crops.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça