RESUMO
BACKGROUND: The increased prevalence of syphilis in Cuba prompted us to map the circulating Treponema pallidum subsp. pallidum allelic profiles in this geographic region. METHODS: Samples were collected from 2012 to 2017, from 83 male patients with ulcers or skin lesions, and were examined using multilocus sequence typing. Additionally, we analyzed the 23S rDNA and 16S rDNA regions for the presence of possible mutations leading to macrolide and tetracycline resistance. RESULTS: Among 94% of fully typed strains, we found 7 different allelic profiles, of which 4 had not been previously described. More than 87% of patients were infected with the T. pallidum SS14-like group and only 8.2% with T. pallidum Nichols-like group. As in other countries, the 1.3.1 allelic profile (ie, SS14-like) was the most common. In addition, 1 of the newly described allelic profiles represents T. pallidum strains that arose by recombination events between members of different T. pallidum subgroups. More than 90% of patients were infected with treponemes harboring the A2058G mutation. However, we found no potential tetracycline-resistant T. pallidum mutations. CONCLUSIONS: Our results suggest that, in Cuba, tetracycline antibiotics could be used to treat syphilis in penicillin-allergic patients instead of macrolides.
Assuntos
Sífilis/microbiologia , Treponema pallidum/classificação , Treponema pallidum/genética , Adulto , Alelos , Antibacterianos , Técnicas de Tipagem Bacteriana , Cuba , Farmacorresistência Bacteriana/genética , Humanos , Macrolídeos/uso terapêutico , Masculino , Tipagem de Sequências Multilocus , Mutação , Sífilis/tratamento farmacológico , Tetraciclina/uso terapêuticoRESUMO
La Dermatitis Digital (DD) es una afección podal de distribución mundial. Al igual que otras enfermedades que afectan al pie bovino induce evidentes cojeras, con las consecuentes pérdidas productivas y económicas. Comúnmente se la describe en el ganado lechero y raramente en ganado de carne. En este trabajo se presentan dos brotes de DD en novillos de feedlot del Noroeste Argentino. Se destacan aspectos clínicos, epidemiológicos, patológicos y de diagnóstico. La incidencia fue de 5 y 35 %. Las lesiones se identificaron típicamente en el área palmar de los miembros posteriores e histopatológicamente se observó una dermatitis necrotizante con infiltrado neutrofílico. Mediante la tinción Warthin-Starry se identificaron espiroquetas intralesionales en el 50% (4/8) de las muestras colectadas.
Digital Dermatitis (DD) is a podal condition of worldwide distribution. Like other diseases that affect the bovine foot, it induces obvious lameness, with consequent productive and economic losses. It is commonly described in dairy cattle and descriptions in beef cattle are scarce. In this paper, two DD outbreaks are described in steers from feed-lot in the Argentine Northwest. Emphasis is given to more relevant aspects of clinical, epidemiological, pathological and diagnostic findings. The incidence was 5 and 35%. Lesions are typically identified in the palmar area of the hind limbs, and a necrotizing dermatitis with neutrophil infiltrate was observed histopathologically. By Warthin-Starry staining was identified an intralesional spirochetes in 50% (4/8) of collected samples.