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Remote sensing data and numerical simulation are important tools to rebuild any oil spill accident letting to identify its source and trajectory. Through these tools was identified an oil spill that affected Oaxacan coast in October 2022. The SAR images were processed with a standard method included in SNAP software, and the numerical simulation was made using Lagrangian transport model included in GNOME software. With the combining of these tools was possible to discriminate the look-alikes from true oil slicks; which are the main issue when satellite images are used. Obtained results showed that 4.3m3 of crude oil were released into the ocean from a punctual point of oil pollution. This oil spill was classified such as a small oil spill. The marine currents and weathering processes were the main drivers that controlled the crude oil displacement and its dispersion. It was estimated in GNOME that 1.6 m3 of crude oil was floating on the sea (37.2 %), 2.4 m3 was evaporated into the atmosphere (55.8 %) and 0.3 m3 reached the coast of Oaxaca (7 %). This event affected 82 km of coastline, but the most important touristic areas as well as turtle nesting zones were not affected by this small crude oil spill. Results indicated that the marine-gas-pump number 3 in Salina Cruz, Oaxaca, is a punctual point of oil pollution in the Southern Mexican Pacific Ocean. Further work is needed to assess the economic and ecological damage to Oaxacan coast caused by this small oil spill.
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Poluição por Petróleo , Petróleo , Poluição por Petróleo/análise , Monitoramento Ambiental/métodos , Tecnologia de Sensoriamento Remoto , Petróleo/análise , Tempo (Meteorologia)RESUMO
The Ecuadorian Amazon is home to a rich biodiversity of woody plant species. Nonetheless, their conservation remains difficult, as some areas remain poorly explored and lack georeferenced records. Therefore, the current study aims predominantly to analyze the collection patterns of timber species in the Amazon lowlands of Ecuador and to evaluate the conservation coverage of these species in protected areas. Furthermore, we try to determine the conservation category of the species according to the criteria of the IUCN Red List. We identified that one third of the timber species in the study area was concentrated in three provinces due to historical botanical expeditions. However, a worrying 22.0% of the species had less than five records of presence, and 29.9% had less than ten records, indicating a possible underestimation of their presence. In addition, almost half of the species evaluated were unprotected, exposing them to deforestation risks and threats. To improve knowledge and conservation of forest biodiversity in the Ecuadorian Amazon, it is recommended to perform new botanical samplings in little-explored areas and digitize data in national herbaria. It is critical to implement automated assessments of the conservation status of species with insufficient data. In addition, it is suggested to use species distribution models to identify optimal areas for forest restoration initiatives. Effective communication of results and collaboration between scientists, governments, and local communities are key to the protection and sustainable management of forest biodiversity in the Amazon region.
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ABSTRACT BACKGROUND: The relationship between spirituality and health has been the object of growing discussion. There is a lack of data on spiritual needs assessments in Brazil. OBJECTIVE: This study aimed to assess the spiritual needs of patients admitted to a public tertiary hospital and perform a comparative analysis between patients with and without indications for palliative care. DESIGN AND SETTING: A cross-sectional observational study included patients hospitalized between August and December 2020 in Hospital do Servidor Publico Municipal, Sao Paulo, Brazil. METHODS: The included patients answered a questionnaire consisting of sociodemographic data, the Duke religiosity scale, and the Spiritual Needs Assessment for Patients (SNAP) tool for a spiritual needs assessment. The World Health Organization Palliative Needs tool (NECPAL) was used to evaluate the indications for palliative care. The level of significance adopted was 5%. RESULTS: A total of 66 patients were included in this study. Most participants (97%) declared themselves as belonging to a religion. The group without indication for palliative care by the NECPAL showed greater spiritual (P = 0.043) and psychosocial needs (P = 0.004). No statistically significant difference was observed in the religious needs domain (P = 0.176). There were no statistically significant differences in the Duke scale scores between the two groups. CONCLUSION: Spiritual, psychosocial, and religious needs are prevalent among hospitalized patients, and multidisciplinary teams must consider these needs in their management approach. In addition, this study suggests that psychosocial and spiritual needs can be even higher in patients who do not receive palliative care.
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OBJECTIVE: To assess the feasibility of a clinical-community direct referral model to enroll eligible households in the Supplemental Nutrition Assistance Program (SNAP). METHODS: Pediatric clinics screening for food insecurity (nâ¯=â¯27) invited families experiencing food insecurity to participate in a direct referral to a local organization that assists with SNAP applications. A food stamp specialist telephoned participants to determine SNAP eligibility, assist with the application, and/or provide other supports. Referrals, eligibility determination, enrollment, and estimated benefits were tracked. RESULTS: A total of 486 families were referred to the community partner; 72% (nâ¯=â¯351) were successfully contacted by a food stamp specialist, with 17% (nâ¯=â¯83) applying for SNAP benefits. Another 16% (nâ¯=â¯79) were already enrolled in SNAP but received an additional service. CONCLUSIONS AND IMPLICATIONS: This referral model was feasible and increased the number of families who received nutrition assistance. This approach could be adapted for other health-related social needs.
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Assistência Alimentar , Abastecimento de Alimentos , Criança , Insegurança Alimentar , Humanos , Pobreza , Encaminhamento e ConsultaRESUMO
The Swanson, Nolan, and Pelham scale version IV (SNAP-IV) is widely used to assess symptoms of attention deficit hyperactivity disorder (ADHD) and oppositional defiant disorder (ODD) in children and adolescents. Nevertheless, there is insufficient data to support its use in preschool children. The study had three goals: First, to test the factorial validity of the three correlated-factors model of ADHD and ODD items of the SNAP-IV. Second, to investigate the measurement invariance of the items over time (6-month longitudinal interval) and by sex. Third, to investigate the convergent validity and method-specific influences on ADHD/ODD assessments with respect to multiple raters (parents/teachers) of children's symptoms. Participants were 618 preschool children (3.5-6 years) at baseline and 6-month follow-up. For model testing, we used confirmatory factor analysis for categorical observed variables. Method and trait effects were examined using the CT-C(M-1) model. The analyses showed partial measurement invariance over time and according to sex. Moreover, strong rater-specific effects were detected. The implication of the results for construct validation of the instrument and clinical assessment of ADHD and ODD traits are discussed.
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Transtorno do Deficit de Atenção com Hiperatividade , Transtornos de Deficit da Atenção e do Comportamento Disruptivo , Adolescente , Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Transtornos de Deficit da Atenção e do Comportamento Disruptivo/diagnóstico , Pré-Escolar , Análise Fatorial , Humanos , PaisRESUMO
OBJECTIVE: To investigate whether single nucleotide polymorphisms (SNPs) in the ADGRL3, DRD4, and SNAP25 genes are associated with and predict ADHD severity in families from a Caribbean community. METHOD: ADHD severity was derived using latent class cluster analysis of DSM-IV symptomatology. Family-based association tests were conducted to detect associations between SNPs and ADHD severity latent phenotypes. Machine learning algorithms were used to build predictive models of ADHD severity based on demographic and genetic data. RESULTS: Individuals with ADHD exhibited two seemingly independent latent class severity configurations. SNPs harbored in DRD4, SNAP25, and ADGRL3 showed evidence of linkage and association to symptoms severity and a potential pleiotropic effect on distinct domains of ADHD severity. Predictive models discriminate severe from non-severe ADHD in specific symptom domains. CONCLUSION: This study supports the role of DRD4, SNAP25, and ADGRL3 genes in outlining ADHD severity, and a new prediction framework with potential clinical use.
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Transtorno do Deficit de Atenção com Hiperatividade , Receptores Acoplados a Proteínas G/genética , Receptores de Peptídeos/genética , Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Transtorno do Deficit de Atenção com Hiperatividade/genética , Manual Diagnóstico e Estatístico de Transtornos Mentais , Humanos , Aprendizado de Máquina , Fenótipo , Polimorfismo de Nucleotídeo Único/genética , Receptores de Dopamina D4/genética , Proteína 25 Associada a Sinaptossoma/genéticaRESUMO
Introducción: Las enfermedades que afectan la articulación de la cadera son numerosas, y dentro de las variantes de tratamiento quirúrgico se encuentra la artroscopia, la que ha ganado gran popularidad en la actualidad por sus ventajas. Objetivo: Actualizar la información sobre la artroscopia de cadera como modalidad de tratamiento quirúrgica mínimo-invasiva en relación con sus indicaciones y contraindicaciones. Métodos: Se realiza una búsqueda y análisis de la información sobre el tema en el período comprendido entre el primero de septiembre y el 31 de octubre de 2020. Se emplearon las siguientes palabras para la búsqueda: hip arthroscopy, snapping hip syndrome, impingement hip síndrome, y a partir de la información obtenida se revisaron 307 artículos publicados en las bases de datos PubMed, Hinari, SciELO y Medline, mediante el gestor de búsqueda y administrador de referencias EndNote. De ellos se seleccionaron 47 citas de los últimos cinco años para la revisión. Análisis y síntesis de la información: Se hace referencia a los trabajos publicados que abordan información sobre la artroscopia de cadera. Se analizan las indicaciones tanto en entidades intrarticulares como periarticulares, con especial énfasis en las primeras y el choque femoroacetabular. Con relación a las contraindicaciones se dividen en absolutas y relativas para su mejor comprensión. Conclusiones: La artroscopia de la cadera es un método quirúrgico útil en una gran variedad de afecciones de la articulación y sus ventajas son numerosas. Esta modalidad quirúrgica permite el diagnóstico de entidades difíciles de detectar por los métodos imagenológicos disponibles en la actualidad y por ende el tratamiento efectivo desde etapas tempranas con el propósito de conservar la articulación(AU)
Introduction: The diseases that affect the hip joint are numerous, and among the variants of surgical treatment is arthroscopy, which has gained great popularity today due to its advantages. Objective: To bring up to date the information on hip arthroscopy as a minimally invasive surgical treatment modality in relation to its indications and contraindications. Methods: A search and analysis of information on the subject was carried out from September 1 to October 31, 2020. The following search words were used: hip arthroscopy, snapping hip syndrome, impingement hip syndrome. Founded on the information obtained, 307 articles published in PubMed, Hinari, SciELO and Medline databases were reviewed, using the EndNote search manager and reference manager. Forty-seven citations of the total were selected for this review. They were published in the last five years. Analysis and synthesis of the information: Reference is made to published papers that address information on hip arthroscopy. Indications for both intra-articular and peri-articular entities are analyzed, with special emphasis on the former and femoroacetabular impingement. In relation to the contraindications, they are divided into absolute and relative for their better understanding. Conclusions: Arthroscopy of the hip is a useful surgical method in wide variety of joint conditions and its advantages are numerous. This surgical modality allows the diagnosis of entities that are difficult to detect by currently available imaging methods and therefore effective treatment from early stages in order to preserve the joint(AU)
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Humanos , Artroscopia , Contraindicações de Procedimentos , QuadrilRESUMO
In plants, vesicular trafficking is crucial for the response and survival to environmental challenges. The active trafficking of vesicles is essential to maintain cell homeostasis during salt stress. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are regulatory proteins of vesicular trafficking. They mediate membrane fusion and guarantee cargo delivery to the correct cellular compartments. SNAREs from the Qbc subfamily are the best-characterized plasma membrane SNAREs, where they control exocytosis during cell division and defense response. The Solanum lycopersicum gene SlSNAP33.2 encodes a Qbc-SNARE protein and is induced under salt stress conditions. SlSNAP33.2 localizes on the plasma membrane of root cells of Arabidopsis thaliana. In order to study its role in endocytosis and salt stress response, we overexpressed the SlSNAP33.2 cDNA in a tomato cultivar. Constitutive overexpression promoted endocytosis along with the accumulation of sodium (Na+) in the vacuoles. It also protected the plant from cell damage by decreasing the accumulation of hydrogen peroxide (H2O2) in the cytoplasm of stressed root cells. Subsequently, the higher level of SlSNAP33.2 conferred tolerance to salt stress in tomato plants. The analysis of physiological and biochemical parameters such as relative water content, the efficiency of the photosystem II, performance index, chlorophyll, and MDA contents showed that tomato plants overexpressing SlSNAP33.2 displayed a better performance under salt stress than wild type plants. These results reveal a role for SlSNAP33.2 in the endocytosis pathway involved in plant response to salt stress. This research shows that SlSNAP33.2 can be an effective tool for the genetic improvement of crop plants.
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Degranulation, a fundamental effector response from mast cells (MCs) and platelets, is an example of regulated exocytosis. This process is mediated by SNARE proteins and their regulators. We have previously shown that several of these proteins are essential for exocytosis in MCs and platelets. Here, we assessed the role of the SNARE protein SNAP23 using conditional knockout mice, in which SNAP23 was selectively deleted from either the megakaryocyte/platelet or connective tissue MC lineages. We found that removal of SNAP23 in platelets results in severe defects in degranulation of all three platelet secretory granule types, i.e., alpha, dense, and lysosomal granules. The mutation also induces thrombocytopenia, abnormal platelet morphology and activation, and reduction in the number of alpha granules. Therefore, the degranulation defect might not be secondary to an intrinsic failure of the machinery mediating regulated exocytosis in platelets. When we removed SNAP23 expression in MCs, there was a complete developmental failure in vitro and in vivo. The developmental defects in platelets and MCs and the abnormal translocation of membrane proteins to the surface of platelets indicate that SNAP23 is also involved in constitutive exocytosis in these cells. The MC conditional deletant animals lacked connective tissue MCs, but their mucosal MCs were normal and expanded in response to an antigenic stimulus. We used this mouse to show that connective tissue MCs are required and mucosal MCs are not sufficient for an anaphylactic response.
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Anafilaxia/imunologia , Plaquetas/imunologia , Tecido Conjuntivo/imunologia , Mastócitos/imunologia , Proteínas Qb-SNARE/imunologia , Proteínas Qc-SNARE/imunologia , Anafilaxia/genética , Anafilaxia/patologia , Animais , Plaquetas/patologia , Tecido Conjuntivo/patologia , Exocitose/genética , Exocitose/imunologia , Mastócitos/patologia , Camundongos , Camundongos Knockout , Proteínas Qb-SNARE/genética , Proteínas Qc-SNARE/genética , Vesículas Secretórias/genética , Vesículas Secretórias/imunologiaRESUMO
ABSTRACT Background: Exocytosis-related gene variants have been suggested to be associated with externalizing behaviors. Objective: This study aimed to examine VAMP2 26 bp Ins/Del, synaptotagmin XI (Syt11) rs3820594 and 33-bp promoter, Syntaxin 1A (Syn-1A) rs1569061 and SNAP-25 rs1051312 and rs3746544 polymorphisms, their serum levels and their relationship with impulsivity, temperament in individuals with alcohol dependence (AD) and healthy controls (HC). Methods: The study included 107 individuals with AD and 104 HCs. Single-nucleotide polymorphisms (SNPs) were studied with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and serum levels with ELISA. Michigan Alcohol Screening Test (MAST), Barratt Impulsiveness Scale-11 (BIS-11) and Temperament Evaluation of Memphis, Pisa, Paris and San Diego Autoquestionnaire (TEMPS-A) were applied. Results: Syn-1A rs1569061 C allele polymorphism was significantly higher in AD group. Syn-1A rs1569061 C allele was associated with 1.5 times increased risk of AD. All serum levels were significantly higher in the HC group. There was a relationship between Syn-1A rs1569061 polymorphism and BIS-11 motor impulsiveness in the AD group; Syt11 rs3820594 polymorphism and BIS-11 total, TEMPS-A depressive, hyperthymia in the HC group. Discussion: In our study, gene variants and serum levels of synaptic vesicle and presynaptic plasma membrane proteins were related to AD, impulsivity and temperament.
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OBJECTIVES: Although child mortality has declined significantly in recent decades, the reduction of neonatal mortality remains a major challenge as neonatal mortality represents 2/3 of the mortality rate in this population. The objective of this study was to evaluate the utility of the Score for Neonatal Acute Physiology Perinatal Extension II (SNAP-PE II) score for evaluating the survival prognosis of newborns admitted to a neonatal intensive care unit (NICU). METHODS: The study design involved an observational cross-sectional retrospective collection, as well as a prospective component. The sample included all newborns admitted to the NICU validated by the SNAP-PE II tool from January 1 to December 31, 2014. RESULTS: A predominance of young mothers (25.4 years), underwent prenatal care (86.2%), however a considerable percentage (49.4%) of mothers received insufficient medical consultation (less than six consults during their pregnancy). A prevalence of male admissions (62.4%) were noted in the NICU. Premature (61.7%) and underweight (weight <2,500 grams) newborns were also prevalent. The SNAP-PE II score showed an association between the infants who were discharged from the neonatal unit and the non-survivors. An increased prevalence of low birth weight and hypothermia was noted in the group of non-survivors. The mean arterial pressure appears to be a significant risk factor in the newborn group that progressed to death. Hypothermia, mean arterial pressure, and birth weight were the most significant variables associated with death. CONCLUSION: The SNAP-PE II was a beneficial indicator of neonatal mortality. The prevention of prematurity and hypothermia by improving maternity care and newborn care can decisively influence neonatal mortality.
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Humanos , Masculino , Feminino , Gravidez , Recém-Nascido , Lactente , Criança , Serviços de Saúde Materna , Prognóstico , Índice de Gravidade de Doença , Estudos Transversais , Estudos Prospectivos , Estudos Retrospectivos , Fatores de RiscoRESUMO
Attention Deficit Hyperactivity Disorder (ADHD) is a highly heritable and prevalent neurodevelopmental disorder that frequently persists into adulthood. Strong evidence from genetic studies indicates that single nucleotide polymorphisms (SNPs) harboured in the ADGRL3 (LPHN3), SNAP25, FGF1, DRD4, and SLC6A2 genes are associated with ADHD. We genotyped 26 SNPs harboured in genes previously reported to be associated with ADHD and evaluated their potential association in 386 individuals belonging to 113 nuclear families from a Caribbean community in Barranquilla, Colombia, using family-based association tests. SNPs rs362990-SNAP25 (T allele; p = 2.46 × 10-4), rs2282794-FGF1 (A allele; p = 1.33 × 10-2), rs2122642-ADGRL3 (C allele, p = 3.5 × 10-2), and ADGRL3 haplotype CCC (markers rs1565902-rs10001410-rs2122642, OR = 1.74, Ppermuted = 0.021) were significantly associated with ADHD. Our results confirm the susceptibility to ADHD conferred by SNAP25, FGF1, and ADGRL3 variants in a community with a significant African American component, and provide evidence supporting the existence of specific patterns of genetic stratification underpinning the susceptibility to ADHD. Knowledge of population genetics is crucial to define risk and predict susceptibility to disease.
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Transtorno do Deficit de Atenção com Hiperatividade/genética , Haplótipos/genética , Polimorfismo de Nucleotídeo Único/genética , Adolescente , Adulto , Negro ou Afro-Americano/genética , Estudos de Casos e Controles , Criança , Colômbia , Feminino , Fator 1 de Crescimento de Fibroblastos/genética , Predisposição Genética para Doença , Humanos , Masculino , Receptores Acoplados a Proteínas G/genética , Receptores de Peptídeos/genética , Proteína 25 Associada a Sinaptossoma/genéticaRESUMO
Mexican bean beetle, Epilachna varivestis Mulsant (Coleoptera: Coccinellidae), is a serious pest of snap bean, Phaseolus vulgaris L. (Fabales: Fabaceae), lima bean, Phaseolus lunatus L. (Fabales: Fabaceae), and an occasional pest of soybean, Glycine max (L.) Merr. (Fabales: Fabaceae), in the United States. Past research indicates that some bean cultivars endure greater injury from this pest than others, suggesting that such crops are either more preferred, better hosts, and/or have poorer defenses. This study characterizes injury potential among three snap bean and three lima bean cultivars by measuring ovipositional preference, survival, and feeding injury of E. varivestis in field and greenhouse experiments. Snap beans, as a group, were more preferred and susceptible to injury than lima beans. The purple wax snap bean, Dragon's Tongue, was the most susceptible overall; while the dwarf bush lima bean, Henderson, was the least susceptible. The results of this study will aid in the development of integrated pest management strategies for E. varivestis, while also providing broader biological information for future research on this pest and others.
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Besouros , Phaseolus , Animais , Produtos Agrícolas , Glycine maxRESUMO
Objetivo: Este artículo compara 4 escalas de predicción de mortalidad y gravedad de la enfermedad (CRIB, CRIB II, SNAPPE, SNAPPE II) en recién nacidos prematuros y a términos, ingresados a las unidades de cuidaos intensivos neonatales (UCIN) para determinar cuál de ellas, tiene mayor discriminación pronostica. Métodos: es un estudio transversal, observacional, multicéntrico para comparar varias escalas de predicción de mortalidad y de la enfermedad. Se recolectaron datos de 227 recién nacidos ingresados a las UCIN de 4 hospitales desde julio a diciembre del 2018. Evaluamos las escalas CRIB, CRIB II, SNAPII y SNAP-PE score en recién nacidos prematuros y a término. El área bajo la curva (ROC) fue usada para evaluar y comparar los resultados de predicción de mortalidad y morbilidad. Resultados: Un total of 227 recién nacidos fueron evaluados (media CRIB: 7,81±3,52 media CRIB-II: 11,96±3,91; media SNAP-II: 34,99±16,83, SNAPPE II: 14,61±13,30). Se evidenció una mayor discriminación para las escalas CRIB II y CRIB en relación con SNAP-II y SNAPP II (AUC 0.94 y 0.93 vs 0.86 y 0,77). Además de cada puntuación, varias variables influyeron significativamente en la supervivencia en los modelos de regresión logística. Conclusiones: Todas las escalas de predicción de mortalidad y de gravedad de la enfermedad sirven para utilizarse en las UCIN estudiadas, siendo la escala CRIB II la de mejor rendimiento para aplicarse en nuestro medio.
Objective: This article compares 4 scales of prediction of mortality and disease severity (CRIB, CRIB II, SNAPPE, SNAPPE II) in preterm and term new borns admitted to neonatal intensive care units (NICU) to determine which of them has greater forecast discrimination. Methods: it is a cross-sectional, observational, multicenter study that compares several mortality and disease prediction scales. Data were collected from 227 newborns admitted to the NICU of 4 hospitals from July to December 2018. We evaluated the CRIB, CRIB II, SNAPII and SNAP-PE score scales in preterm and full term infants. The area under the curve (ROC) was used to evaluate and compare the prediction results of mortality and morbidity. Results: A total of 227 newborns were evaluated (mean CRIB: 7.81 ± 3.52 mean CRIB-II: 11.96 ± 3.91, average SNAP-II: 34.99 ± 16.83, SNAPPE II: 14.61 ± 13.30). There was evidence of greater discrimination for the CRIB II and CRIB scales in relation to SNAP-II and SNAPP II (AUC 0.94 and 0.93 vs 0.86 and 0.77). In addition to each score, several variables significantly influenced survival in the logistic regression models. Conclusions: All the prediction scales of mortality and severity of the disease serve to be used in the studied NICUs, being the CRIB II scale the best performance to apply in our environment.
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Humanos , Recém-Nascido , Recém-Nascido Prematuro , Mortalidade Infantil , Previsões , Recém-NascidoRESUMO
Abstract Background: Synaptosomal-associated protein 25 (SNAP-25) may be contribute to the pathogenesis of fibromyalgia Syndrome (FMS) by affecting the release of neurotransmitters. Objectives: We aimed to investigate the relationship between the SNAP-25 gen (DdeI = rs1051312 and MnlI = rs3746544) polymorphism and the temperament and character traits. Methods: A total of 85 female patients diagnosed with FMS and 70 age-matched healthy female subjects were enrolled into the study. The Temperament and Character Inventory (TCI) were performed on all the patients. SNAP-25 gene polymorphism was determined in the patients group and controls group. Results: No significant difference between groups was found regarding the distribution of SNAP-25 MnlI polymorphism (p > 0.05), but it was seen that the frequency of TC genotype for DdeI gene was higher in the patients group (p < 0.05). Increased hazard avoidance was found in the patients group (p < 0.05). When TCI scores were assessed in terms of SNAP-25 gene polymorphism, no statistically significant relationship was detected between the TT, TG, GG genotypes for MnlI gen and TCI scores (p > 0.05). However, increased hazard avoidance was detected in patients with TC genotype for DdeI gene compared to patients without such genotype. Discussion: SNAP-25 might be an etiological factor in FMS pathogenesis and might affect personality traits of FMS patients by mediating neurotransmitter release.
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SPG11 mutations are the major cause of autosomal recessive Hereditary Spastic Paraplegia. The disease has a wide phenotypic variability indicating many regions of the nervous system besides the corticospinal tract are affected. Despite this, anatomical and phenotypic characterization is restricted. In the present study, we investigate the anatomical abnormalities related to SPG11 mutations and how they relate to clinical and cognitive measures. Moreover, we aim to depict how the disease course influences the regions affected, unraveling different susceptibility of specific neuronal populations. We performed clinical and paraclinical studies encompassing neuropsychological, neuroimaging, and neurophysiological tools in a cohort of twenty-five patients and age matched controls. We assessed cortical thickness (FreeSurfer software), deep grey matter volumes (T1-MultiAtlas tool), white matter microstructural damage (DTI-MultiAtlas) and spinal cord morphometry (Spineseg software) on a 3â¯T MRI scan. Mean age and disease duration were 29 and 13.2â¯years respectively. Sixty-four percent of the patients were wheelchair bound while 84% were demented. We were able to unfold a diffuse pattern of white matter integrity loss as well as basal ganglia and spinal cord atrophy. Such findings contrasted with a restricted pattern of cortical thinning (motor, limbic and parietal cortices). Electromyography revealed motor neuronopathy affecting 96% of the probands. Correlations with disease duration pointed towards a progressive degeneration of multiple grey matter structures and spinal cord, but not of the white matter. SPG11-related hereditary spastic paraplegia is characterized by selective neuronal vulnerability, in which a precocious and widespread white matter involvement is later followed by a restricted but clearly progressive grey matter degeneration.
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Gânglios da Base/diagnóstico por imagem , Mutação , Proteínas/genética , Paraplegia Espástica Hereditária/genética , Substância Branca/diagnóstico por imagem , Adolescente , Adulto , Imagem de Tensor de Difusão , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neuroimagem , Testes Neuropsicológicos , Paraplegia Espástica Hereditária/diagnóstico por imagem , Adulto JovemRESUMO
An outbreak of cyclosporiasis in Ontario, Canada, was investigated in the fall of 2015. Thirty-five confirmed and 10 probable cases were linked to the investigation. Epidemiological and food safety evidence implicated fresh sugar snap peas imported from Guatemala as the source of the outbreak. We describe here the first documented cyclosporiasis outbreak in Canada involving the consumption of sugar snap peas.
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Ciclosporíase/epidemiologia , Pisum sativum/parasitologia , Cyclospora , Surtos de Doenças , Guatemala , Humanos , Ontário/epidemiologiaRESUMO
In a study in Costa Rica 314 serum samples from dogs throughout all seven provinces were tested using a commercial kit for the detection of circulating antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato and Ehrlichia canis, and of circulating antigen of Dirofilaria immitis. A total of 6.4% (20/314) and 38.2% (120/314) were positive for Anaplasma spp. (An) and E. canis (Ec) antibodies. Overall, 8.0% (25/314) were positive for D. immitis (Di) antigen. One single dog reacted positive with B. burgdorferi s.l. (Bb) antigen (0.3%, 1/314). E. canis positive dogs were detected in all provinces (highest percentages in Guanacaste, Puntarenas [both significantly different compared to the overall] and Limón). Guanacaste and Puntarenas also showed the highest prevalences of Anaplasma spp. (both significantly different compared to the overall). The highest prevalence of D. immitis was detected in Puntarenas (significantly different compared to the overall). Double pathogen exposure (Ec plus An; Ec plus Di; Ec plus Bb) were recorded in 8.9% (28/314). Two dogs showed a triple pathogen exposure (0.6%, 2/314; An, Ec and Di). There was a significant difference between male (11.5%, 18/156) and female (4.4%, 7/158) animals for D. immitis positive results. There was also a significant difference between breed and no breed dogs regarding the characteristics of a general positive test, as well as seropositivity to the single pathogens of Anaplasma spp., E. canis and D. immitis. Finally there was a significant difference in the presence of clinical signs again regarding the characteristics of a general positive test, as well as seropositivity to Anaplasma spp., E. canis and D. immitis. Practitioners in Costa Rica should be aware of the canine vector-borne diseases mentioned as dogs are at risk of becoming infected. Concerning the positive B. burgdorferi s.l. dog, an autochthonous occurrence cannot be confirmed due to a history of adoption and an unusual tattoo number. Veterinary advice to protect dogs and limit transmission of vector-borne pathogens, also to humans, by using prophylactic measures is strongly recommended.
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Anaplasmose/epidemiologia , Dirofilariose/epidemiologia , Doenças do Cão/epidemiologia , Ehrlichiose/veterinária , Doença de Lyme/veterinária , Fatores Etários , Anaplasma/isolamento & purificação , Anaplasmose/genética , Anaplasmose/microbiologia , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Helmintos/sangue , Grupo Borrelia Burgdorferi/isolamento & purificação , Costa Rica/epidemiologia , Dirofilaria immitis/isolamento & purificação , Dirofilariose/genética , Dirofilariose/parasitologia , Doenças do Cão/genética , Doenças do Cão/microbiologia , Doenças do Cão/parasitologia , Cães , Ehrlichia canis/isolamento & purificação , Ehrlichiose/epidemiologia , Ehrlichiose/genética , Ehrlichiose/microbiologia , Feminino , Doença de Lyme/epidemiologia , Doença de Lyme/genética , Doença de Lyme/microbiologia , Masculino , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Fatores SexuaisRESUMO
OBJECTIVE: Few studies assessed factors associated with the agreement/disagreement between fathers and mothers when rating ADHD symptoms of their offspring. METHOD: Teachers and both parents assessed a referred sample of 98 children and adolescents aged 6 to 16 years ( M age = 9.79, SD = 2.59) using the Swanson, Nolan, and Pelham (SNAP-IV) rating scale. The agreement was assessed for each of the items of the scale and correlated with variables measuring children's features, socioeconomic adversity, family functioning, and parental psychopathology. RESULTS: Mean agreement between parents was moderate for the inattentive and good for the hyperactive-impulsive construct. Mothers tended to report more symptoms than fathers. The agreement was lower in those families where parents had discrepant educational levels. CONCLUSION: Our findings suggest a significant cross-informant disagreement between parents on symptoms of ADHD. Discrepant parental education has a relevant role in explaining parental disagreement in reporting ADHD symptoms.
Assuntos
Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Docentes/psicologia , Pai/psicologia , Mães/psicologia , Adolescente , Adulto , Transtorno do Deficit de Atenção com Hiperatividade/psicologia , Criança , Escolaridade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Relações Pais-Filho , Pais/psicologia , Reprodutibilidade dos Testes , Instituições Acadêmicas , Avaliação de SintomasRESUMO
Mexican bean beetle, Epilachna varivestis Mulsant, is a serious pest of snap beans, Phaseolus vulgaris L., in the eastern United States. These beetles are intolerant to direct sunlight, explaining why individuals are typically found on the undersides of leaves and in the lower portion of the plant canopy. We hypothesized that snap beans grown on reflective, agricultural polyethylene (plastic mulch) would have fewer Mexican bean beetles and less injury than those grown on black plastic or bare soil. In 2014 and 2015, beans were seeded into beds of metallized, white, and black plastic, and bare soil, in field plots near Blacksburg, VA. Mexican bean beetle density, feeding injury, predatory arthropods, and snap bean yield were sampled. Reflected light intensity, temperature, and humidity were monitored using data loggers. Pyranometer readings showed that reflected light intensity was highest over metallized plastic and second highest over white plastic; black plastic and bare soil were similarly low. Temperature and humidity were unaffected by treatments. Significant reductions in Mexican bean beetle densities and feeding injury were observed in both metallized and white plastic plots compared to black plastic and bare soil, with metallized plastic having the fewest Mexican bean beetle life stages and injury. Predatory arthropod densities were not reduced by reflective plastic. Metallized plots produced the highest yields, followed by white. The results of this study suggest that growing snap beans on reflective plastic mulch can suppress the incidence and damage of Mexican bean beetle, and increase yield in snap beans.