RESUMO
It is important to determine the number of plants to be evaluated to allow accurate inferences about the traits under evaluation. Investigating the linear relations among traits is important for identifying traits for indirect selection. So, the objectives of this study were to determine the sample size (number of plants) necessary to estimate the means of forage pea traits and to investigate the relations among the traits. Experiments were carried out in 2021 with three sowing dates (May 3, May 26 and July 13). Five hundred plants were randomly sampled, 100 plants in each of the five evaluation dates (June 25, August 30, July 24, September 17, September 16). In these 500 plants, the traits plant height, number of branches, number of nodes, number of leaves, number of pods, fresh matter of leaves, fresh matter of stems, fresh matter of pods, fresh matter of shoots, dry matter of leaves, dry matter of stems, dry matter of pods, and dry matter of shoots, were evaluated. The sample size was calculated to estimate the means of these traits, based on Student's t-distribution, and the relations among traits were investigated through correlation and path analysis. In an experiment, to estimate the means of these 13 traits of forage pea, with an estimation error of approximately 10% of the mean, 99 plants per treatment should be sampled. The numbers of pods and leaves have a positive linear relations with fresh and dry matter of shoots.
É importante dimensionar o número de plantas a serem avaliadas para possibilitar inferências precisas sobre os caracteres em avaliação. Investigar as relações lineares entre caracteres é importante para a identificação de caracteres para a seleção indireta. Assim, os objetivos deste trabalho foram determinar o tamanho de amostra (número de plantas) necessário para a estimação da média de caracteres de ervilha forrageira e investigar as relações entre os caracteres. Foram conduzidos experimentos, no ano de 2021, em três datas de semeadura (03 de maio, 26 de maio e 13 de julho). Foram amostradas, aleatoriamente, 500 plantas, sendo 100 plantas em cada uma das cinco datas de avaliação (25 de junho, 30 de agosto, 24 de julho, 17 de setembro e 16 de setembro). Nessas 500 plantas avaliaram-se os caracteres altura de planta, número de ramificações, número de nós, número de folhas, número de legumes, matéria fresca de folhas, matéria fresca de caule, matéria fresca de legumes, matéria fresca de parte aérea, matéria seca de folhas, matéria seca de caule, matéria seca de legumes e matéria seca de parte aérea. Foi calculado o tamanho de amostra para a estimação da média desses caracteres, com base na distribuição t de Student e investigada a relação entre os caracteres por meio de análises de correlação e de trilha. Em um experimento, para a estimação da média desses 13 caracteres de ervilha forrageira, com erro de estimação de aproximadamente 10% da média, devem ser amostradas 99 plantas por tratamento. Os números de legumes e de folhas têm relação linear positiva com as matérias fresca e seca de parte aérea.
Assuntos
Tamanho da Amostra , Pisum sativum , Melhoramento VegetalRESUMO
Approximately four million people contract fungal infections every year in Brazil, primarily caused by Aspergillus spp. The ability of these fungi to form biofilms in tissues and medical devices complicates treatment and contributes to high rates of morbidity and mortality in immunocompromised patients. Psd2 is a pea defensin of 5.4 kDa that possesses good antifungal activity against planktonic cells of representative pathogenic fungi. Its function depends on interactions with membrane and cell wall lipid components such as glucosylceramide and ergosterol. In the present study, we characterized Aspergillus nidulans biofilm formation and determined the effect of Psd2 on A. nidulans biofilms. After 4 hours, A. nidulans conidia adhered to polystyrene surfaces and formed a robust extracellular matrix-producing biofilm at 24 h, increasing thickness until 48 h Psd2 inhibited A. nidulans biofilm formation in a dose-dependent manner. Most notably, at 10 µM Psd2 inhibited 50% of biofilm viability and biomass and 40% of extracellular matrix production. Psd2 significantly decreased the colonized surface area by the biofilm and changed its level of organization, causing a shortening of length and diameter of hyphae and inhibition of conidiophore formation. This activity against A. nidulans biofilm suggests a potential use of Psd2 as a prototype to design new antifungal agents to prevent biofilm formation by A. nidulans and related species.
RESUMO
Biofortification refers to an approach to increase micronutrient concentrations in the edible parts of plants with increased bioavailability to the human population. Conventional, agronomic and transgenic breeding methods can be used to develop these biofortified crops, offering sustainable and cost-effective strategies. Pea has long been recognized as a valuable, nutritious food for the human diet, but there is a limited amount of information about it, which prevents the full micronutrient enrichment potential of this pulse crop to be reached. Considerations must include not only micronutrient concentrations but also the amount of the nutrient that can be absorbed by the consumer, after processing and cooking. Development of biofortified pea that retains nutrients during cooking and processing is not only essential for fighting micronutrient malnutrition, but also necessary to improve agricultural productivity. © 2021 Society of Chemical Industry.
Assuntos
Alimentos Fortificados/análise , Pisum sativum/química , Animais , Biofortificação , Humanos , Micronutrientes/análise , Micronutrientes/metabolismo , Pisum sativum/metabolismo , Sementes/química , Sementes/metabolismoRESUMO
Field peas (Pisum sativum L.) are widely cultivated throughout the world as a cool season grain and forage crop. Boron (B) toxicity is caused by high B concentration in the soil or irrigation water, and is particularly problematic in medium or heavier textured soil types with moderate alkalinity and low annual rainfall. Previous studies have indicated that B-toxicity increases oxidative stress in plants, and B-tolerance has been considered an important target in field pea plant breeding programmes. Inducers of tolerance may be a promising alternative for plant breeding. Little research has been conducted on the combined use of silicon (Si) and salicylic acid (SA) to remediate B-toxicity in field peas. The present study revealed the physiological and biochemical plant responses of applying Si + SA under B-toxicity (15 mg B L-1) on two Brazilian field pea cultivars (Iapar 83 and BRS Forrageira). A semi-hydroponic experiment was conducted using a completely randomized factorial design (2 × 5): with two field pea cultivars and five treatments which were formed by individual and combined applications of Si and SA under B-toxicity plus a control (control, B, B + Si, B + SA, and B + Si + SA). Si (2 mmol L-1) was applied to plants in two forms (root and leaf), while for SA (36⯵molâ¯L-1) only foliar applications were applied. Our results demonstrated that the combined use of exogenous Si + SA in field peas increased tolerance to B-toxicity through an intensified antioxidant plant defence system, resulting in a better regulation of reactive oxygen species (ROS) production and degradation. It significantly increased total chlorophyll and carotenoids contents, the activities of major antioxidant enzymes, and reduced MDA and H2O2 contents, resulting in increased fresh shoot and total plant dry biomass. The application of Si + SA alleviated the inhibitory effects of boron toxicity in field peas, resulting in greater plant growth by preventing oxidative membrane damage through an increased tolerance to B-excess within the plant tissue. Therefore, the use of Si + SA is an important and sustainable strategy to alleviate B-toxicity in field pea cultivation.
Assuntos
Antioxidantes/metabolismo , Boro/toxicidade , Pisum sativum/fisiologia , Ácido Salicílico/metabolismo , Silício/metabolismo , Poluentes do Solo/toxicidade , Brasil , Clorofila/metabolismo , Peróxido de Hidrogênio/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Pisum sativum/efeitos dos fármacos , Folhas de Planta/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
RESUMEN Los agroecosistemas muestran una gran heterogeneidad en cuanto a las prácticas y características, debido a que dependen de las lógicas de la agricultura familiar, convirtiéndose en un recurso potencialmente valioso, cuando se estudia el uso y el soporte del medio productivo. Es por ello, que esta investigación evaluó el sistema de arveja (Pisum sativum L.) en el municipio de Ipiales, a través de una metodología multidimensional, usando 26 indicadores, agrupados en cuatro dimensiones de sustentabilidad agroecológica. La estimación, se realizó sobre 30 fincas, que representaron tres tipos de campesinos, previamente descritos, como C1, C2 y C3; los resultados permitieron identificar los Puntos Críticos de Sustentabilidad (PCS) y el Índice General de Sustentabilidad (IGS) del sistema, estableciendo que las diferentes situaciones sociales, ambientales, económicas y técnico-productivas determinan la existencia de PCS específicos. El IGS tuvo una calificación de 2,65, para C1; 2,90, para C2 y 2,62, para C3, evidenciando medidas de monitoreo y de manejo, que se deberían discutir en la región, para disminuir las externalidades generadas por el modelo productivo.
ABSTRACT Agroecosystems present great heterogeneity in terms of practices and characteristics, since they depend on the logic of family farming, which makes them a potentially valuable resource when studying the use and support of the productive environment. For this reason, this research evaluated the pea system (Pisum sativum L.) in the municipality of Ipiales, through a multidimensional methodology that uses 26 indicators grouped into four dimensions of agroecological sustainability. The estimation was made on 30 farms that represented three types of farmers previously described as C1, C2 and C3; the results allowed identifying the Critical Points of Sustainability (CPS) and the General Index of Sustainability (GIS) of the system. It was established that the different social, environmental, economic and technical-productive situations determine the existence of specific CPS, the GIS had a rating of 2.65 for C1, 2.90 for C2 and 2.62 for C3, this evidenced monitoring and management measures that should be discussed in the region to reduce the externalities generated by the production model.
RESUMO
Psd1 is a pea plant defensin which can be actively expressed in Pichia pastoris and shows broad antifungal activity. This activity is dependent on fungal membrane glucosylceramide (GlcCer), which is also important for its internalization, nuclear localization, and endoreduplication. Certain cancer cells present a lipid metabolism imbalance resulting in the overexpression of GlcCer in their membrane. In this work, in vitroassays using B16F10 cells showed that labeled fluorescein isothiocyanate FITC-Psd1 internalized into live cultured cells and targeted the nucleus, which underwent fragmentation, exhibiting approximately 60% of cells in the sub-G0/G1 stage. This phenomenon was dependent on GlcCer, and the participation of cyclin-F was suggested. In a murine lung metastatic melanoma model, intravenous injection of Psd1 together with B16F10 cells drastically reduced the number of nodules at concentrations above 0.5 mg/kg. Additionally, the administration of 1 mg/kg Psd1 decreased the number of lung inflammatory cells to near zero without weight loss, unlike animals that received melanoma cells only. It is worth noting that 1 mg/kg Psd1 alone did not provoke inflammation in lung tissue or weight or vital signal losses over 21 days, inferring no whole animal cytotoxicity. These results suggest that Psd1 could be a promising prototype for human lung anti-metastatic melanoma therapy.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Defensinas/farmacologia , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/secundário , Pisum sativum/química , Proteínas de Plantas/farmacologia , Animais , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Biópsia , Linhagem Celular , Permeabilidade da Membrana Celular , Proliferação de Células/efeitos dos fármacos , Defensinas/química , Modelos Animais de Doenças , Feminino , Imunofluorescência , Glucosilceramidas/metabolismo , Imuno-Histoquímica , Neoplasias Pulmonares/tratamento farmacológico , Melanoma Experimental , Camundongos , Modelos Moleculares , Proteínas de Plantas/química , Conformação Proteica , Relação Estrutura-AtividadeRESUMO
Pisum sativum defensin 2 (Psd2) is a small (4.7 kDa) antifungal peptide whose structure is held together by four conserved disulfide bridges. Psd2 shares the cysteine-stabilized alpha-beta (CSαß) fold, which lacks a regular hydrophobic core. All hydrophobic residues are exposed to the surface, except for leucine 6. They are clustered in the surface formed by two loops, between ß1 and α-helix and ß2 and ß3 sheets. The observation of surface hydrophobic clusters reveals a remarkable evolution of the CSαß fold to expose and reorganize hydrophobic residues, which facilitates creating versatile binding sites.
Assuntos
Defensinas/química , Pisum sativum/química , Proteínas de Plantas/química , Interações Hidrofóbicas e Hidrofílicas , Modelos Moleculares , Ressonância Magnética Nuclear Biomolecular , Conformação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Dobramento de ProteínaRESUMO
Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.
A ervilha forrageira é uma importante cultura anual de clima frio, suas sementes contêm altos níveis de aminoácidos essenciais, que são carentes na composição de grãos de cereais, e por isso, são empregadas como fonte nutritiva de forragem por criadores de gado. Para implantação dos campos dessa forrageira é necessário o conhecimento da qualidade das sementes, pelo teste de germinação e os de vigor. Na literatura trabalhos sobre testes de vigor para sementes de ervilha forrageira são escassos. Este trabalho teve como objetivo verificar a eficiência de testes de vigor, com ênfase no envelhecimento acelerado para a avaliação da qualidade fisiológica de sementes de ervilha forrageira visando seleção de lotes para a emergência de plântulas em campo. Foram utilizados 10 lotes de ervilha forrageira e avaliados o grau de umidade, massa de mil sementes, porcentagem de emergência, primeira contagem de emergência e índice de velocidade de emergência de plântulas em campo, germinação, primeira contagem de germinação, condutividade elétrica e teste de envelhecimento acelerado com as seguintes variações de metodologia: temperaturas de 41 e 42 °C; tempos de exposição de 48 e 72 horas e diferentes umidades na atmosfera de envelhecimento, mediante a utilização de água destilada (100% UR), solução saturada de cloreto de potássio (87% UR) e cloreto de sódio (76% UR). Os testes germinação, primeira contagem de emergência de plântulas em campo, índice de velocidade de emergência e condutividade elétrica são promissores para avaliar o vigor de lotes de sementes de ervilha forrageira. O teste de envelhecimento acelerado conduzido em solução saturada com cloreto de potássio a 42 °C por 48 horas é adequado para estimar o vigor de lotes de sementes de ervilha forrageira, fornecendo informações semelhantes à emergência de plântulas em campo.
Assuntos
Pisum sativum/crescimento & desenvolvimento , Sementes , Produtos AgrícolasRESUMO
Forage pea is an important annual, cold climate crop with high levels of essential amino acids in its seeds, which are lacking in the composition of cereal grains. Therefore, livestock farmers use its seeds as a nutritious source of forage. To plant fields of this forage, it is necessary to know the quality of the seeds, determined through germination and vigor tests. In the literature, studies on vigor tests for forage pea seeds are scarce. This study aimed to verify the efficiency of vigor tests, with emphasis on accelerated aging to evaluate the physiological quality of forage pea seeds in order to select lots for seedling emergence in the field. Using accelerated aging tests, 10 lots of forage pea were evaluated for moisture content, thousand-seed weight, emergence percentage, emergence first count, speed index of seedlings in the field, germination, germination first count, and electrical conductivity. The following variations of methodology were used: temperatures of 41 and 42 °C, exposure times of 48 and 72 h, and different relative humidity values in the aging atmosphere, using distilled water (100 % RH), saturated solution of potassium chloride (87 % RH), and sodium chloride (76 % RH). The tests of germination, emergence first count of seedlings in the field, emergence speed index, and electrical conductivity are promising to evaluate the vigor of forage pea seed lots. The accelerated aging test conducted in a solution saturated with potassium chloride at 42 °C for 48 h is adequate to estimate the vigor of forage pea seed lots, providing information similar to that of the emergence of seedlings in the field.(AU)
A ervilha forrageira é uma importante cultura anual de clima frio, suas sementes contêm altos níveis de aminoácidos essenciais, que são carentes na composição de grãos de cereais, e por isso, são empregadas como fonte nutritiva de forragem por criadores de gado. Para implantação dos campos dessa forrageira é necessário o conhecimento da qualidade das sementes, pelo teste de germinação e os de vigor. Na literatura trabalhos sobre testes de vigor para sementes de ervilha forrageira são escassos. Este trabalho teve como objetivo verificar a eficiência de testes de vigor, com ênfase no envelhecimento acelerado para a avaliação da qualidade fisiológica de sementes de ervilha forrageira visando seleção de lotes para a emergência de plântulas em campo. Foram utilizados 10 lotes de ervilha forrageira e avaliados o grau de umidade, massa de mil sementes, porcentagem de emergência, primeira contagem de emergência e índice de velocidade de emergência de plântulas em campo, germinação, primeira contagem de germinação, condutividade elétrica e teste de envelhecimento acelerado com as seguintes variações de metodologia: temperaturas de 41 e 42 °C; tempos de exposição de 48 e 72 horas e diferentes umidades na atmosfera de envelhecimento, mediante a utilização de água destilada (100% UR), solução saturada de cloreto de potássio (87% UR) e cloreto de sódio (76% UR). Os testes germinação, primeira contagem de emergência de plântulas em campo, índice de velocidade de emergência e condutividade elétrica são promissores para avaliar o vigor de lotes de sementes de ervilha forrageira. O teste de envelhecimento acelerado conduzido em solução saturada com cloreto de potássio a 42 °C por 48 horas é adequado para estimar o vigor de lotes de sementes de ervilha forrageira, fornecendo informações semelhantes à emergência de plântulas em campo.(AU)
Assuntos
Pisum sativum/crescimento & desenvolvimento , Sementes , Produtos AgrícolasRESUMO
RESUMEN La arveja, una fuente económica para pequeños y medianos agricultores de la zona andina, evidencia un incipiente manejo pos-cosecha y un nulo nivel de transformación. Tras la cosecha y debido a su naturaleza temporal y perecedera, la arveja acelera su metabolismo, disminuyendo su calidad y vida útil. Una opción económica y que esté disponible para los productores y LOS comercializadores para conservar sus características es el uso de empaques y la refrigeración. El objetivo fue evaluar tipos de empaques (bolsa de polietileno de baja densidad -PE-LD-, envase plástico termoformado perforado -PE-HD- y bandeja de poliestireno -PS-) y temperaturas de almacenamiento (0°C, 4°C y 18°C) en dos variedades de arveja, Sureña y Obonuco Andina. Se utilizó un diseño irrestrictamente al azar con arreglo factorial 3x3x2, teniendo como factores temperatura, empaque y variedad y como variables de estudio, las constantes cinéticas (K) de porcentaje de brotación, firmeza, pérdida de peso e índice de color. Los resultados mostraron que, para la brotación, se presentó el valor más bajo de K en Sureña, en PE-LD, a 0°C. La temperatura afectó la firmeza de los granos, conservándose a 0°C. La menor pérdida de peso se evidenció en PE-HD, a 0°C, independiente de la variedad. Para el índice de color, la K más baja se encontró en PS, a 0°C, en Obonuco Andina. Con la ecuación de Arrhenius en la variable índice de color, se estimó tiempos de vida útil de 13; 7; 5 y 2 días a temperaturas de 0°C, 6°C, 10°C y 24°C, respectivamente.
ABSTRACT The pea, an economic source for small and medium farmers in the Andes zone, evidences an incipient postharvest handling and a null level of transformation. After the harvest and due to its temporary and perishable nature, the pea accelerates its metabolism, decreasing its quality and shelf life. An economic option that is available to producers and marketers to preserve their characteristics is the use of packaging and refrigeration. The objective was to evaluate types of packaging (low density polyethylene bag (PE-LD), perforated thermoformed plastic container (PE-HD) and polystyrene tray (PS)) and storage temperatures (0°C, 4°C and 18°C) in two pea varieties (Sureña and Obonuco Andina). An unrestricted random design with a 3x3x2 factorial arrangement was used, taking as factors temperature, packing and variety; and as study variables the kinetic constants (K) of percentage of sprouting, firmness, weight loss and color index. The results showed that, for the sprouting, the lowest value of K was shown in Sureña, in PE-LD at 0°C. The temperature affected the firmness of the grains, remaining at 0°C. The lowest weight loss was evidenced in PE-HD at 0°C, regardless of the variety. For the color index, the lowest K was found in PS at 0°C, in Obonuco Andina. With the Arrhenius equation in the variable color index, useful life times of 13; 7; 5 and 2 days were estimated at temperatures of 0°C, 6°C, 10°C and 24°C respectively.
RESUMO
Seeds presents variable germination performance at different temperatures and substrates, which are basic components of germination test. Within this context, this work aimed to adapt the methodology for the seeds germination's test of P. sativum subsp. arvense. The experiment required four portions of seeds, five temperature degrees (15, 20, 25, 20-30 and 15-25 °C), and two substrata (roll of paper and in sand). Seeds evaluation considered the following: moisture degree and germination test. The effect of seed portion, substrata and temperature in germination was assessed on a daily basis. It was calculated the rate of germination, of anormal plants, of dormant and dead seeds, and of speed of germination index; initial, final and average times, and synchrony. The experimental design consisted of random blocks with four replications, while conjoint analysis was the statistical procedure adopted. The germination test for forage pea seeds should be conducted at the constant temperature 20 °C in roll of paper substratewith the first count and final count on the 4th and 7th day, respectively.
As sementes apresentam desempenho germinativo variável em diferentes temperaturas e substratos, que são componentes básicos do teste de germinação. Dentro deste contexto, este trabalho teve como objetivo adequar a metodologia para o teste de germinação de sementes de P. sativum subsp. arvense. Para tanto, foram utilizados quatro lotes de sementes, cinco temperaturas (15, 20, 25, 20-30 e 15-25 °C) e dois substratos (rolo de papel e entre areia). O teor de água das sementes foi determinado antes das avaliações e o efeito dos lotes, substratos e temperaturas sobre a germinação foi avaliado diariamente. Calculou-se a porcentagem de germinação, de plântulas anormais, de sementes dormentes e mortas, o índice de velocidade de germinação, os tempos inicial, final e médio e sincronia. O delineamento adotado foi o de blocos casualizados, com quatro repetições e o procedimento estatístico adotado foi análise conjunta. O teste de germinação deve ser conduzido na temperatura constante de 20 °C, em substrato rolo de papel com primeira contagem e contagem final ao 4° e 7° dia, respectivamente.
Assuntos
Sementes , Substratos para Tratamento Biológico , Pisum sativum , GerminaçãoRESUMO
Psd2 is a pea defensin with 47 amino acid residues that inhibits the growth of fungal species by an uncharacterized mechanism. In this work, Psd2 interactions with model membranes mimicking the lipid compositions of different organisms were evaluated. Protein-lipid overlay assays indicated that Psd2 recognizes Fusarium solani glucosylceramide (GlcCerF.solani) and ergosterol (Erg) in addition to phosphatidylcholine (POPC) and some phosphatidylinositol species, such as PtdIns (3)P, (5)P and (3,5)P2, suggesting that these lipids may play important roles as Psd2 targets. Assays using lipid vesicles were also performed to study the behaviour and dynamics that occur after peptide-membrane interactions. Surface plasmon resonance analysis showed that Psd2 has a higher affinity for pure POPC and POPC-based vesicles containing GlcCer and Erg at a 70:30 proportion than for vesicles containing cholesterol (Chol). Partition experiments by fluorescence spectroscopy showed a decrease in Trp42 quantum yield of Psd2 in the presence of GlcCerF.solani and Erg, individually or in simultaneously enriched membranes. The partition coefficient (Kp) obtained indicated a Psd2 partition preference for this vesicles, confirmed by quenching assays using acrylamide and 5/16-doxyl-stearic acid. Furthermore, we showed that the presence of C8C9 double bonds and a methyl group at position C9 of the sphingoid base backbone of GlcCer was relevant to Psd2 activity against Aspergillus nidulans. These results are consistent with the selectivity of Psd2 against fungi and its lack of toxicity in human erythrocytes. Psd2 represents a promising natural compound for the treatment of fungal infections.
Assuntos
Defensinas/química , Ergosterol/química , Glucosilceramidas/química , Microdomínios da Membrana/química , Membranas Artificiais , Proteínas de Plantas/química , Pisum sativum/químicaRESUMO
Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases.
RESUMO
O objetivo deste trabalho foi estudar a ervilha Luciana 50, cultivada sob diferentes números de fileiras de plantas no canteiro e com duas e três sementes por cova. O trabalho foi desenvolvido em Dourados-MS, entre março e julho de 2010. Os tratamentos foram arranjados no esquema fatorial 3 x 2 no delineamento experimental de blocos casualizados, com seis repetições. As populações correspondentes à semeadura com duas, três e quatro fileiras no canteiro foram de 264.000, 396.000 e 528.000 plantas ha-1, respectivamente, com duas sementes por cova, e de 396.000, 594.000 e 792.000 plantas ha-1, respectivamente, com três sementes por cova. A colheita foi realizada aos 108 dias após a semeadura. No cultivo com quatro fileiras de plantas e duas sementes por cova, obtiveram-se as maiores produções de massa fresca e massa seca de grãos e vagem comercial, com aumentos de 29,88%, 33,85%, 29,14% e 32,22%, respectivamente, e maiores números de grãos e vagens comerciais, com aumentos de 28,13% e 27,12%, respectivamente, em relação a duas fileiras de plantas com duas sementes por cova. As maiores produções de massa fresca da parte aérea, de cascas e de vagens não-comerciais foram com quatro fileiras de plantas, com aumentos de 1,75 Mg ha-1, 0,44 Mg ha-1 e 0,47 Mg ha-1 respectivamente, em relação a duas fileiras. Considerando a produtividade de vagens e grãos comerciais e a estimativa da renda líquida, pode-se concluir que a semeadura de ervilha Luciana 50 deve ser realizada com quatro fileiras de plantas e com duas sementes por cova.(AU)
The aim of this work was to study the luciana 50 pea, cultivated with different numbers of rows of plants in the plot and with two and three seeds per hill. The work carried out in Dourados-MS, between March-July 2010. Treatments were arranged in 3 x 2 factorial in randomized complete block design with six replicates. Populations corresponding to the sowing with two, three and four rows per plot were 264,000, 396,000 and 528,000 plants ha-1, respectively, with two seeds per hill, and 396,000, 594,000 and 792,000 plants ha-1, respectively, with three seeds per hill. The harvest was done at 108 days after sowing. In cultivation with four rows of plants and two seeds per hill, were obtained the highest yields of fresh and dry weight of grains and pods commercial, with increases of 29.88%, 33.85%, 29.14% and 32.22%, respectively, and higher number of grains and pods commercial, with increases of 28.13% and 27.12%, respectively, over two rows of plants with two seeds per hill. The highest yield of fresh weight of shoots, of bark and of non-commercial pods were with four rows of plants, with increases of 1.75 Mg ha-1, 0.44 Mg ha-1 and 0.47 Mg ha-1 respectively, compared to two rows. Considering the yield of commercial pods and grains and the estimated net income, it can be concluded that sowing of Luciana 50 pea should be performed with four rows of plants and two seeds per hill.(AU)
Assuntos
Pisum sativum , 24444 , Produtos AgrícolasRESUMO
Psd1 is a plant defensin that has antifungal activity against several pathogenic and nonpathogenic fungi. Previous analysis of Psd1 chemical shift perturbations by nuclear magnetic resonance (NMR) spectroscopy demonstrated that this defensin interacts with phospholipids and the sphingolipid glucosylceramide isolated from Fusarium solani (GlcCer(Fusarium solani)). In this study, these interactions were evaluated by real-time surface plasmon resonance (SPR) analysis. The data obtained demonstrated that Psd1 could bind more strongly to small unilamellar vesicles (SUV)-containing GlcCer(Fusarium solani) than to SUV that was composed of phosphatidylcholine (PC) alone or was enriched with GlcCer that had been isolated from soybeans. An increase in the SPR response after cholesterol or ergosterol incorporation in PC-SUV was detected; however, SUV composed of PC:Erg (7:3; molar:molar) became unstable in the presence of Psd1, suggesting membrane destabilization. We also observed a lack of Psd1 internalization in Candida albicans strains that were deficient in the glucosyl ceramide synthase gene. Together, these data indicate that GlcCer is essential for Psd1 anchoring in the fungal plasma membrane as well as internalization.
Assuntos
Candida albicans/fisiologia , Defensinas/metabolismo , Glucosilceramidas/metabolismo , Lipossomos/metabolismo , Proteínas de Plantas/metabolismo , Ressonância de Plasmônio de Superfície , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Defensinas/farmacologia , Endocitose/efeitos dos fármacos , Cinética , Testes de Sensibilidade Microbiana , Microscopia Confocal , Fosfatidilcolinas/metabolismo , Proteínas de Plantas/farmacologiaRESUMO
The genus Leptolyngbya comprises filamentous cyanobacteria that are important in rice fields. In the rhizosphere, cyanobacteria produce a variety of secondary metabolites such as auxins that are important in agriculture soil performance. To assess this, Leptolyngbya strain MMG-1, was isolated from the rhizosphere of rice plants and described. For this, the morphology of this strain was studied by light microscopy as well as by confocal laser scanning microscopy. Besides, the ability of this strain to synthesize an auxin-like bioactive compound was demonstrated under various culture conditions (different amounts of tryptophan; pH; different alternating light:dark periods; duration of the incubation). The auxin-like compound was extracted from the culture of Leptolyngbya strain MMG-1 and identified as indole-3-acetic acid (IAA) by thin layer chromatography (TLC) as well as by high performance liquid chromatography (HPLC). Our results showed that the strain required the precursor L-tryptophan for the synthesis of IAA. Leptolyngbya strain MMG-1 accumulated IAA intracellularly. The IAA secreted by Leptolyngbya strain MMG-1 was significantly correlated with the initial concentration of L-tryptophan in the medium, as well as with the duration of the incubation. The bioactivity of the secreted IAA was determined by its effect on the rooting pattern of Pisum sativum seedlings. The culture supernatant of Leptolyngbya strain MMG-1 stimulated the seedling lateral rooting, while it decreased root length. Hence, rhizospheric Leptolyngbya produced auxin under different conditions and affected the plants rooting pattern. Rev. Biol. Trop. 62 (3): 1251-1260. Epub 2014 September 01.
El género Leptolyngbya comprende cianobacterias filamentosas que son importantes en los campos de cultivo de arroz. En la rizosfera, las cianobacterias producen una variedad de metabolitos secundarios, tales como auxinas, que son importantes en el rendimiento de la agricultura del suelo. La cepa Leptolyngbya MMG-1, fue aislada de la rizosfera de plantas de arroz y se describe en este trabajo. La morfología de esta cepa se estudió por microscopía de luz, así como por microscopía confocal de barrido láser. Además, se estimó la capacidad de esta cepa para sintetizar el compuesto bioactivo auxina como se demostró en diversas condiciones de cultivo (diferentes cantidades de triptófano; pH; diferente luz alterna: períodos oscuros; duración de la incubación). La auxina se extrajo a partir del cultivo de la cepa Leptolyngbya MMG-1 y se identificó como ácido indol-3-acético (AA) por cromatografía de capa fina (TLC), así como por cromatografía líquida de alta resolución (HPLC). Nuestros resultados mostraron que la cepa requiere el precursor de L-triptófano para la síntesis de IAA. La cepa Leptolyngbya MMG-1 acumula intracelularmente IAA. El IAA secretada por la cepa Leptolyngbya MMG-1 se correlacionó significativamente con la concentración inicial de L-triptófano en el medio, así como con la duración de la incubación. La bioactividad de la IAA secretada se determinó por su efecto sobre el patrón de enraizamiento de plantas de semillero de Pisum sativum. El sobrenadante del cultivo de la cepa Leptolyngbya MMG-1 estimuló el enraizamiento lateral en la plántula, mientras que se redujo la longitud de la raíz. Por lo tanto, la producción de auxina por Leptolyngbya rizosférica afectó el crecimiento de las plantas.
Assuntos
Cianobactérias/fisiologia , Ácidos Indolacéticos/isolamento & purificação , Oryza/microbiologia , Cianobactérias/química , Cianobactérias/classificação , Cianobactérias/isolamento & purificaçãoRESUMO
O objetivo deste trabalho foi estudar a ervilha Luciana 50, cultivada sob diferentes números de fileiras de plantas no canteiro e com duas e três sementes por cova. O trabalho foi desenvolvido em Dourados-MS, entre março e julho de 2010. Os tratamentos foram arranjados no esquema fatorial 3 x 2 no delineamento experimental de blocos casualizados, com seis repetições. As populações correspondentes à semeadura com duas, três e quatro fileiras no canteiro foram de 264.000, 396.000 e 528.000 plantas ha-1, respectivamente, com duas sementes por cova, e de 396.000, 594.000 e 792.000 plantas ha-1, respectivamente, com três sementes por cova. A colheita foi realizada aos 108 dias após a semeadura. No cultivo com quatro fileiras de plantas e duas sementes por cova, obtiveram-se as maiores produções de massa fresca e massa seca de grãos e vagem comercial, com aumentos de 29,88%, 33,85%, 29,14% e 32,22%, respectivamente, e maiores números de grãos e vagens comerciais, com aumentos de 28,13% e 27,12%, respectivamente, em relação a duas fileiras de plantas com duas sementes por cova. As maiores produções de massa fresca da parte aérea, de cascas e de vagens não-comerciais foram com quatro fileiras de plantas, com aumentos de 1,75 Mg ha-1, 0,44 Mg ha-1 e 0,47 Mg ha-1 respectivamente, em relação a duas fileiras. Considerando a produtividade de vagens e grãos comerciais e a estimativa da renda líquida, pode-se concluir que a semeadura de ervilha Luciana 50 deve ser realizada com quatro fileiras de plantas e com duas sementes por cova.
The aim of this work was to study the luciana 50 pea, cultivated with different numbers of rows of plants in the plot and with two and three seeds per hill. The work carried out in Dourados-MS, between March-July 2010. Treatments were arranged in 3 x 2 factorial in randomized complete block design with six replicates. Populations corresponding to the sowing with two, three and four rows per plot were 264,000, 396,000 and 528,000 plants ha-1, respectively, with two seeds per hill, and 396,000, 594,000 and 792,000 plants ha-1, respectively, with three seeds per hill. The harvest was done at 108 days after sowing. In cultivation with four rows of plants and two seeds per hill, were obtained the highest yields of fresh and dry weight of grains and pods commercial, with increases of 29.88%, 33.85%, 29.14% and 32.22%, respectively, and higher number of grains and pods commercial, with increases of 28.13% and 27.12%, respectively, over two rows of plants with two seeds per hill. The highest yield of fresh weight of shoots, of bark and of non-commercial pods were with four rows of plants, with increases of 1.75 Mg ha-1, 0.44 Mg ha-1 and 0.47 Mg ha-1 respectively, compared to two rows. Considering the yield of commercial pods and grains and the estimated net income, it can be concluded that sowing of Luciana 50 pea should be performed with four rows of plants and two seeds per hill.
Assuntos
24444 , Pisum sativum , Produtos AgrícolasRESUMO
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (< 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid desiccation was also associated with higher activities and levels of malate dehydrogenase and the oxidized form of nicotinamide adenine dinucleotide. It was also accompanied by lower hydroperoxide levels and membrane damage. In addition, the activitiy of glutathione reductase was greater during rapid drying. Ageing may have contributed to increased damage during slow dehydration, since viability declined even in wet storage after two weeks. The results presented are consistent with rapid desiccation reducing the accumulation of damage resulting from desiccation-induced aqueous-based deleterious reactions. In addition, they show that radicles are a useful model to study desiccation sensitivity in plant tissues.
Assuntos
Dessecação/métodos , Peroxidação de Lipídeos/fisiologia , Pisum sativum/fisiologia , Raízes de Plantas/metabolismo , Sementes/fisiologia , Água/metabolismo , Antioxidantes/metabolismo , Germinação/fisiologia , NAD , Estresse Oxidativo , Oxirredutases/metabolismo , Pisum sativum/metabolismo , Fosfofrutoquinases/metabolismo , Sobrevivência de Tecidos/fisiologiaRESUMO
Sugar snap pea (Pisum sativum L. var. macrocarpon Ser.) is an edible-podded sweet pea that is being considered as a new totally mechanized crop to supply raw material to the agri-food industry of the Ebro Valley (Northern Spain). It is of great interest from an agronomic and commercial standpoint but neither its agronomic behaviour nor its adaptation to the area are known. The objective of this study was to determine the effect of sowing date and seed density on the growth and yield of the sugar snap pea at industrial scale. Six randomized blocks experiments with four replicates were conducted on irrigated land in Villafranca (Navarra, Spain) in 1998, 1999, and 2000. Three experiments for testing sowing dates (Mar., Apr., and May) and another three for seed densities (from 75 to 150 plants m-2) were performed. Phenological development, thermal integral and qualitative and quantitative yield controls were performed. Sugar snap pea required 960 ºC d-1 (Tb = 3 ºC) from sowing to harvest. The early sowings gave more biomass, but yield was similar. However, Harvest Index and crop morphology varied. The sowing densities had similar yields sowing that sugar snap pea has a bigger adaptation availability. Sugar snap peas can be satisfactorily cultivated at industrial scale in the zone with sowings between Mar. and May and with seeding densities between 75 and 150 plants m-2.
RESUMO
Sugar snap pea (Pisum sativum L. var. macrocarpon Ser.) is an edible-podded sweet pea that is being considered as a new totally mechanized crop to supply raw material to the agri-food industry of the Ebro Valley (Northern Spain). It is of great interest from an agronomic and commercial standpoint but neither its agronomic behaviour nor its adaptation to the area are known. The objective of this study was to determine the effect of sowing date and seed density on the growth and yield of the sugar snap pea at industrial scale. Six randomized blocks experiments with four replicates were conducted on irrigated land in Villafranca (Navarra, Spain) in 1998, 1999, and 2000. Three experiments for testing sowing dates (Mar., Apr., and May) and another three for seed densities (from 75 to 150 plants m-2) were performed. Phenological development, thermal integral and qualitative and quantitative yield controls were performed. Sugar snap pea required 960 ºC d-1 (Tb = 3 ºC) from sowing to harvest. The early sowings gave more biomass, but yield was similar. However, Harvest Index and crop morphology varied. The sowing densities had similar yields sowing that sugar snap pea has a bigger adaptation availability. Sugar snap peas can be satisfactorily cultivated at industrial scale in the zone with sowings between Mar. and May and with seeding densities between 75 and 150 plants m-2.