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Triple-negative breast cancer (TNBC) overexpresses the Epidermal Growth Factor Receptor (EGFR), a characteristic of different types of tumors, linked to worse disease prognosis and risk of recurrence. Conventional treatments are also aggressive and can be morbid.. Therefore, t improvement and development of new methods are notorious. Photodynamic Therapy (PDT) is an effective method for treating different types of cancer by using light radiation to activate a photosensitizing agent (drug) in molecular oxygen presence, promoting cell death., Improving drug uptake in target cells could contribute to PDT efficiency. Accordingly, we developed a bifunctional nanoprobe (BN), used in PDT as a a treatment method in vivo against breast cancer. The BN uses gold nanoparticles with active targeting through the Epidermal Growth Factor (EGF) protein and Chlorine e6 (Ce6) carriers. We evaluated the therapeutic efficacy of in vivo xenograft in 4 groups: Saline, BN, Ce6+PDT, and BN+PDT. As a result, we observed that the BN+PDT group exhibited an excellent effect with greater selectivity to tumor tissue and tissue damage when compared to the Saline, BN, and Ce6+PDT groups. The results indicate a potential impact on breast cancer treatment in vivo.. In conclusion, our data propose that the BN developed heightened PDT efficacy through cellular DNA repair effects and tumor microenvironment.

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Introducción: La queilitis actínica (QA) es una lesión pre-neoplásica secundaria al fotodaño solar, que afecta al labio inferior. La Terapia fotodinámica (TFD) es un tratamiento promisorio en QA con buenos resultados tanto clínicos, que se han confirmado con hallazgos histopatológicos, así como cosméticos. Material y método: Serie de casos. Se presentan 3 casos de pacientes de sexo femenino tratados con TFD, en donde se utilizó ácido metil aminolevulínico en forma oclusiva y con protección de la luz 3 horas antes de la aplicación de la luz roja con lámpara Aktilite™, en el Hospital clínico de la Universidad de Chile entre los años 2008 y 2012. Se realizaron biopsias de control al final de la TFD para confirmar curación. Resultados: Se obtuvo el 100 por ciento de respuesta clínica en los 3 pacientes con un máximo de seguimiento de 33 meses en uno, sin evidencia de recidiva clínica ni histopatológica, con escasos efectos colaterales, buena aceptación y conformidad por parte de los pacientes. Conclusión: La TFD es una buena alternativa en el manejo de la QA con excelentes resultados y mínimos efectos colaterales. Los excelentes resultados clínicos se confirman con la histopatología, por lo cual se recomendaría su uso, previa biopsia, que descarte un carcinoma invasor concurrente. Queda aún por definir los intervalos de tratamiento y su duración.

Introduction: Actinic cheilitis (AC) is a pre-neoplasic lesion secondary to sun photodamage, that affect the inferior lip. Photodinamic therapy (PDT) is a promissory way of treatment inAC which has proven to be successful both clinical, and by histopatologic findings, as well as in cosmetic results. Materials and methods: Case series. Three cases are presented; female patients treated with PDT using methyl aminolevulinic acid in an occlusive way and protected from the light three hours before the application of red light using the Aktilite™ lamp. The trial was perfomed at the Clinical hospital of the University Chile of between the years 2008 and 2012. Control biopsies were made at the end of the PDT to confirm resolution. Results: 100 percent of clinical response was obtained in the three patients with a maximal follow-up of 33 months in one of the patients. No evidence of neither clinical nor histopathological reappearance was found fewer side effects, good reception and acceptance from the patients. Conclusion: PDT is a good alternative for the management of the AC with excellent results and minimal side effects. The outstanding clinical results are confirmed by histopathology, suggesting its use prior ruling out concurrent invasive carcinoma. Nevertheless, still reminds to define the treatments intervals and its duration.

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