RESUMO
Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purified enzymes-endo-1,4-ß-xylanase, α-L-arabinofuranosidase, ß-xylosidase, and α-D-glucuronidase-over hydrolysis of glucuronoarabinoxylan (GAX) obtained from brewers' spent grain (BSG) after alkaline extraction and ethanol precipitation. First, monosaccharides, uronic acids and glycosidic-linkages of alkaline extracted GAX fraction from BSG were characterized, after that different strategies based on the addition of one or two families of enzymes-endo-1,4-ß-xylanase (GH10 and GH11) and α-L-arabinofuranosidase (GH43 and GH51)-cooperating with one ß-xylosidase (GH43) and one α-D-glucuronidase (GH67) into enzymatic hydrolysis were assessed to obtain the best yield of xylose. The xylose release was monitored over time in the first 90 min and after a prolonged reaction up to 48 h of reaction. The highest yield of xylose was 63.6% (48 h, 40 â, pH 5.5), using a mixture of all enzymes devoid of α-L-arabinofuranosidase (GH43) family. These results highlight the importance of GH51 arabinofuranosidase debranching enzyme to allow a higher cleavage of the xylan backbone of GAX from BSG and their synergy with 2 endo-1,4-ß-xylanase (GH10 and GH11), one ß-xylosidase (GH43) and the inclusion of one α-D-glucuronidase (GH67) in the reaction system. Therefore, this study provides an environmentally friendly process to produce xylose from BSG through utilization of enzymes as catalysts.
RESUMO
A glucuronoarabinoxylan (CNAL) was extracted with 1% aq. KOH (25°C) from Cocos nucifera gum exudate. It had a homogeneous profile on HPSEC-MALLS-RI (Mw 4.6 × 10(4)g/mol) and was composed of Fuc, Ara, Xyl, GlcpA (and 4-O-GlcpA) in a 7:28:62:3 molar ratio. Methylation data showed a branched structure with 39% of non-reducing end units, 3-O-substituted Araf (8%), 3,4-di-O- (15%), 2,4-di-O- (5%) and 2,3,4-tri-O-substituted Xylp units (17%). The anomeric region of CNAL (13)C NMR spectrum contained 9 signals, indicating a complex structure. The main chain of CNAL was characterized by analysis of a Smith-degraded polysaccharide. Its (13)C NMR spectrum showed 5 main signals at δ 101.6, δ 75.5, δ 73.9, δ 72.5, and δ 63.1 that were attributed to C-1, C-4, C-3, C-2 and C-5 of (1â4)-linked ß-Xylp-main chain units, respectively. CNAL exhibited gastroprotective effect, by reducing gastric hemorrhagic lesions, when orally administered (1 and 3mg/kg) to rats prior to ethanol administration.