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Oxygenated blood is required for the adequate metabolic activity of the brain. This is supplied by the circle of Willis (CoW) and the vertebrobasilar and carotid systems. The CoW ensures blood flow in case of arterial stenosis or occlusion. Different animal models have been explored for the CoW morphological and functional study. This work aims to characterize the vascular architecture of the CoW of the plains vizcacha, Lagostomus maximus (Suborder: Hystricomorpha), and to compare it with evolutionarily related species of Caviomorpha and Muroidea. The blood supply in adult plains vizcachas was studied using latex cerebrovascular casts and angiography. A caudo-rostral flow direction was determined, beginning in the spinal and vertebral arteries and converging in the basilar artery which bifurcates in the carotid-basilar communication in the caudal communicating arteries. In the first third of its course, the caudal cerebral arteries project laterally, and the middle and rostral cerebral arteries bifurcate from their rostral terminal segment, supplying the temporo-parietal and frontal cortex. The CoW architecture is mainly conserved between rodent species. Likewise, the small neurovascular variations observed could be considered phylogenetic morphological variations more than evolutionary adaptations. The absence of the rostral communicating artery that generates the rostral open architecture of the CoW in the vizcacha as in the other analyzed species, supports the need for a revision of the CoW classical function as a security system. Finally, this work supports the importance of expanding our understanding of brain anatomy among species, which may contribute to a better understanding of functional neuroanatomy.

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Viruses comprise the most abundant genetic material in the biosphere; however, global viral genomic population (virome) has been largely underestimated. Recently, high-throughput sequencing (HTS) has provided a powerful tool for the detection of known viruses and the discovery of novel viral species from environmental and individual samples using metagenomics and ecogenomics approaches, respectively. Viruses with circular DNA single-stranded (ssDNA) genomes belonging to the begomovirus genera (family Geminiviridae) constitute the largest group of emerging plant viruses worldwide. The knowledge of begomoviruses viromes is mostly restricted to crop plant systems; nevertheless, it has been described that noncultivated plants specifically at the interface between wild and cultivated plants are important reservoirs leading to viral evolution and the emergence of new diseases. Here we present a protocol that allows the identification and isolation of known and novel begomoviruses species infecting cultivated and noncultivated plant species. The method consists of circular viral molecules enrichment by rolling circle amplification (RCA) from begomovirus-positive total plant DNA, followed by NGS-based metagenomic sequencing. Subsequently, metagenomic reads are processed for taxonomic classification using Viromescan software and a customized Geminiviridae family database, and begomovirus-related reads are used for contigs assembly and annotation using Spades software and Blastn algorithm, respectively. Then, the obtained begomovirus-related signatures are used as templates for specific primers design and implemented for PCR-based ecogenomic identification of individual samples harboring the corresponding viral species. Lastly, full-length begomovirus genomes are obtained by RCA-based amplification from total plant DNA of selected individual samples, cloning, and viral molecular identity corroborated by Sanger sequencing. Conclusively, the identification and isolation of a novel monopartite begomovirus species native to the New World (NW) named Gallium leaf deformation virus (GLDV) is shown.

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La muerte súbita es aquella que ocurre dentro de las 24 horas posteriores al inicio de los síntomas y se caracteriza por ser clínicamente inexplicable, inesperada y repentina. Debido a la naturaleza de la muerte súbita, no es posible llegar a un diagnóstico preciso sin una autopsia. En esta comunicación breve, evaluaremos el caso de un empleado de crucero de 33 años, sin historial médico/farmacológico previo, el cual falleció súbitamente mientras reposaba en su camarote. Debido a las sospechas iniciales de una posible muerte causada por una sobredosis de cocaína, se le realizó un panel toxicológico abarcador el cual resultó negativo. Empero, una tomografía computarizada (TC) craneal sin contraste revirtió la hipótesis inicial y la autopsia neuropatológica -sorpresivamente- confirmó que la verdadera causa de muerte fue la ruptura de un aneurisma sacular desconocido en el polígono de Willis.

Sudden death occurs within 24 hours after the onset of symptoms and is characterized by being clinically inexplicable, sudden, and unexpected. Due to the nature of sudden death, it is not possible an accurate diagnosis without performing an autopsy. In this brief communication, we will evaluate the case of a 33-year-old cruise employee, with no prior medical/pharmacological history, who suddenly died while resting in his cabin. Due to initial suspicions of a possible cocaine overdose death, a comprehensive toxicology panel was performed, although yielding a negative result. A cranial computed tomography without contrast reversed the initial hypothesis and the neuropathological autopsy -surprisingly- confirmed that the true cause of death was the rupture of an unknown saccular aneurysm in the Circle of Willis.

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SUMMARY: The structure formed by the arteries, which is of great importance in the irrigation of the brain, is called the cerebral arterial circle (Polygon of Willis). Since the cerebral arterial circle provides brain nutrition, vascularabnormalities in this region are highly relevant. Therefore, the aim of our study was to examine the variations of the cerebral arterial circle in cadavers of Turkish individuals. In our study, 32 human cadavers obtained from three different universities were retrospectively examined. Brain tissue obtained from cadavers by craniotomy was kept in 20 % formaldehyde solution for an average of 10 days for fixation. Cerebral arterial circle diagrams were determined in all cadavers by staining and photographs were taken. As a result of the brain examinations, a variation of the cerebral arterial circle was detected in 24/32 brains. Vascular variations have an important place in congenital variations. For this reason, we believe that our brain study will contribute to clinical studies on this topic by investigating variations of the cerebral arterial circle.

La estructura formada por las arterias que tiene una gran importancia en la irrigación del cerebro se denomina círculo arterial cerebral (Polígono de Willis). Dado que el círculo arterial cerebral proporciona la irrigación cerebral, las anomalías vasculares en esta región son muy relevantes. Por lo tanto, el objetivo de nuestro estudio fue examinar las variaciones del círculo arterial cerebral en cadáveres de individuos turcos. En nuestro estudio, se examinaron retrospectivamente 32 cadáveres humanos obtenidos de tres universidades diferentes. El tejido cerebral de los cadáveres fue obtenido por craneotomía y se mantuvo en solución de formaldehído al 20 %, durante 10 días en promedio para su fijación. Se determinaron los diagramas de círculo arterial cerebral en todos los cadáveres mediante coloración y se tomaron fotografías. Como resultado de los exámenes, se detectó una variación del círculo arterial cerebral en 24/32 de los cerebros. Las variaciones vasculares tienen un lugar importante en las variaciones congénitas. Por esta razón, creemos que nuestro estudio contribuirá a estudios clínicos sobre el tema al pesquisar las variaciones del círculo arterial cerebral.

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Background: We present five patients with remodeling of the adult circle of Willis in response to flow diverter stents (FDSs) at the anterior communicating artery (AComA) and the posterior communicating artery (PComA). The observed changes provide a paradigm of how flow change can institute anatomic changes in the adult circle of Willis vasculature. Case Description: In the first two cases, after placement of the FDS covering the AComA, there was an increase in size and flow of the contralateral A1-anterior cerebral artery which had previously been hypoplastic. In one of the cases, this led to the filling of the aneurysm and required placement of coils within the lesion which was curative. In case three, the FDS effect led to asymptomatic occlusion of the PComA and associated aneurysm without change of the ipsilateral P1-segement of posterior-cerebral-artery (P1-PCA) caliber. In the fourth case, the FDS covering an aneurysm with a fetal PCA arising from its neck resulted in significant reduction of the aneurysm size, persistent flow and caliber of the fetal PCA, and the hypoplastic ipsilateral P1-PCA. Finally, in the fifth case, after FDS occlusion of the PComA and aneurysm there was increasement in diameter of the ipsilateral P1-PCA that was previously hypoplastic. Conclusion: The use of FDS can affect vessels covered by the device and other arteries of the circle of Willis adjacent to the FDS. The phenomena illustrated in the hypoplastic branches appear to be a compensatory response to the hemodynamic changes induced by the divertor and to the altered flow in the circle of Willis.

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Bovine papillomaviruses are related to cause fibroepithelial proliferations in the skin and mucosae and are associated with economic loss mainly related to poor body condition and reduced milk production. This study aimed to investigate the presence and types of bovine papillomaviruses (BPVs) in cattle sampled in different areas of Costa Rica using molecular techniques. A descriptive study with a non-probability convenience sampling was carried out. A total of 99 papillomatous lesions were collected from 63 animals in 32 farms, and analyzed by polymerase chain reaction, rolling circle amplification (RCA), sequencing, and restriction enzymes digestion. Seven bovine papillomavirus types (BPV1, BPV2, BPV4, BPV6, BPV7, BPV10, BPV11) and two putative novel viral variants (BPV-CR1 and BPV-CR2) were identified for the first time in Costa Rica. BPV6 was the most frequently detected virus in lesions (31.2%), followed by BPV2 (25%) and BPV1 (25%). BPV1 and BPV2 were the most widely distributed in the Country. Coinfections were recorded in two animals (BPV1 / BPV2 and BPV4 / BPV6). Restriction analyses allowed differentiating BPV1 from BPV2, BPV4, and BPV7, but failed to identify BPV6, BPV10, and BPV11. Results suggest that a great PVs diversity is harbored by bovines in Costa Rica and indicate the need for further investigations aimed to uncover PV diversity at the full genomic level.

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The learning process for a Biology topic regarding organisms and animal kingdom diversity was investigated through an innovative Interactive Didactic Sequence (IDS) which integrated the idea of "concept maps" with the Hermeneutic-Dialectic Circle (HDC). HDC is a tool for data collection and a reference for pluralist-constructivist thinking, considered a form of fourth-generation evaluation. Hofstede's cultural dimensions were also integrated into the investigation in order to facilitate mediation in an evaluative context. Students' performances (N = 25) from a São Paulo-Brazil public school were statistically evaluated. Their cultural profile was determined via the Hofstede Value Survey Model 1994 questionnaire. The elaborative process of arranging concept maps was individual (CM-individual) and integrated with HDC in groups (CM-HDC). Concept map assessment methods were based off existing literature. An improvement in students' performances (p < 0.05) that presented concept maps integrated to HDC in a more complex structure when compared to individually-built maps was observed. Employment of HDC helped form motivational/interactive dialogues between students and teachers, which, in turn, assisted in achieving greater learning through the use of concept maps. The application of the fourth-generation evaluation was improved via knowledge regarding students' cultural profiles.

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Torque Teno Virus (TTV) was initially associated with post-transfusion hepatitis, but growing evidence of its ubiquity in humans is compatible to no apparent clinical significance. TTV is a small non-enveloped virus with a circular single-negative-stranded DNA genome, belonging to the Anelloviridae family. Currently, TTVs are divided in seven phylogenetic groups and are further classified into 21 species. Studies about diversity of TTV in different conditions are receiving increasing interest and in this sense, sequencing of whole genomes for better genetic characterization becomes even more important. Since its discovery in 1997, few TTV complete genomes have been reported worldwide. This is probably due, among other reasons, to the great genetic heterogeneity among TTV strains that prevents its amplification and sequencing by conventional PCR and cloning methods. In addition, although metagenomics approach is useful in these cases, it remains a challenging tool for viromic analysis. With the aim of contributing to the expansion of the TTV whole genomes dataset and to study intra-host variants, we employed a methodology that combined a rolling-circle amplification approach followed by EcoRI digestion, generating a DNA fragment of ∼4Kb consistent with TTV genome length which was sequenced by Illumina next generation sequencing. A genogroup 3 full-length consensus TTV genome was obtained and co-infection with other species (at least those with a single EcoRI cleavage site) was not identified. Additionally, bioinformatics analysis allowed to identify the spectrum of TTV intra-host variants which provides evidence of a complex evolution dynamics of these DNA circular viruses, similarly to what occurs with RNA viruses.

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Resumo Com objetivo de analisar e discutir como a dança circular está sendo utilizada na área de saúde, e assim compreender seu potencial terapêutico nos processos de cuidado, procedeu-se a uma revisão bibliográfica a partir das bases de dados científicas PubMed, Scielo, BVS MTCI, Lilacs e Web of Science, buscando a produção sobre o tema. Os artigos encontrados, avaliados pela análise temática, evidenciam as seguintes questões: as relações estabelecidas entre as Práticas Integrativas e Complementares em Saúde (PICS) e a produção de cuidado; o uso da dança circular como estratégia de promoção da saúde física e emocional; processos de criatividade e os sentidos de coletividade vivenciados pelos praticantes da dança circular. Observou-se que a dança circular é capaz de estimular relações mais harmoniosas consigo mesmo e com o coletivo, reduz o estresse e sintomas depressivos, promove a ampliação da consciência corporal, autoconfiança e autonomia, contribuindo para melhorar habilidades cognitivas, psicomotoras, desempenho físico e o equilíbrio, trazendo benéficos como sensação de relaxamento e prazer. Nesse sentido, a literatura aponta evidências sobre efeitos positivos da prática de dança circular em vários resultados relacionados à saúde, podendo ser considerada uma estratégia potente de cuidado.

Abstract A narrative literature review was conducted from the scientific databases PubMed, SciELO, BVS MTCI, LILACS, and Web of Science, searching for production on the topic to analyze and discuss how the circle dance is being used in Health and, thus, understand its therapeutic potential in care. The articles found were evaluated by the thematic analysis and highlighted the following issues: the relationships established between the Integrative and Complementary Practices in Health (PICS) and care production; circle dance as a strategy to promote physical and emotional health; creativity and the senses of collectivity experienced by circle dance practitioners. We observed that the circle dance can stimulate more harmonious relationships with oneself and the community. It reduces stress and depressive symptoms, promotes expanded body awareness, self-confidence, and autonomy, improves cognitive and psychomotor skills, physical performance, and balance, and brings benefits such as a sensation of relaxation and pleasure. In this sense, the literature points to evidence of the positive effects of the circle dance practice on various health-related outcomes, which can be considered a powerful care strategy.

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Abstract To celebrate the 400th anniversary of the birth of Thomas Willis, his main contributions to the development of neurosciences, in particular neurology, are presented. Willis coined the term neurology and contributed significantly to the field of neuroanatomy, with the description of the arterial circle—located at the base of the brain—, which bears his name. He also described the striatum and cranial nerves. Furthermore, as a clinical neurologist, Willis participated in the description of various diseases, including myasthenia gravis and restless legs syndrome.

Resumo Na comemoração dos 400 anos de nascimento de Thomas Willis, são apresentadas as suas principais contribuições para o desenvolvimento das neurociências, em particular a neurologia. Willis cunhou o termo neurologia, contribuiu significativamente na área de neuroanatomia, com a descrição do círculo arterial localizado na base do cérebro, que tem o seu nome, além da descrição do corpo estriado, e de nervos cranianos. Da mesma forma, como neurologista clínico, Willis participou da descrição de várias doenças como a miastenia gravis e da síndrome das pernas inquietas, entre outras doenças.

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SUMMARY: The variations knowledge of the cerebral arterial circle (CAC) is relevant due to its influence on the development of ischemic encephalic disorders. Among these variations, when the external diameter of the posterior communicating artery exceeds the posterior cerebral artery caliber, we have a fetal conformation of this circle. The aim of this study was to describe the variations of the CAC in Chilean individuals and to know the type of arterial conformation. Thirty adult brains were used to measure lengths and caliber of the pre-communicating segments of the anterior (A1) and posterior (P1) cerebral arteries, and the anterior (ACoA) and posterior (PCoA) communicating arteries. The arterial conformation type was established, and the length and caliber of these vessels were compared according to the right or left side. It was observed that 76.6 % of the CACs presented aplasia and / or hypoplasia. Of its components, PCoA was hypoplasic in 53.3 %, appearing bilaterally in 40 % of the subjects. The comparison according to the side, indicated that the mean length of A1 and PCoA on the right side was slightly higher. In the case of caliber, the mean of A1, P1 and PCoA was higher on the left side. Regarding P1 and PcoA caliber, 33 % of the CACs presented unilateral fetal conformation. Regardless of the variability presented by the CAC, there is consensus that PCoA exhibits the greatest variability. The understanding of this variability requires an analysis of the embryonic aspects that can explain the fetal conformation of the CAC in the adult.

RESUMEN: El conocimiento de las variaciones del círculo arterial cerebral (CAC) resultan relevantes por su influencia en el desarrollo de trastornos isquémicos encefálicos. De estas variaciones, los cambios del calibre de la arteria comunicante posterior (ACoP) determinan una conformación fetal de este círculo. El objetivo de este estudio fue describir las variaciones del CAC en individuos chilenos y conocer el tipo de conformación arterial. Se utilizaron 30 encéfalos adultos a los que se midieron las longitudes y calibres de los segmentos precomunicante de las arterias cerebrales anteriores (A1) y posteriores (P1), y de las arterias comunicante anterior (ACoA) y ACoP. Se estableció el tipo de conformación arterial y se comparó la longitud y calibre de estos vasos según lateralidad. Se observó que el 76,6 % de los CAC presentaron agenesias y/o hipoplasias. De sus componentes, la ACoP fue hipoplásica en el 53,3 %, presentándose bilateral en el 40 %. La comparación según lateralidad indicó que la longitud media de A1 y ACoP del lado derecho fueron levemente superiores. En el caso de los calibres, la media de A1, P1 y ACoP fue superior en el lado izquierdo. Respecto de los calibres de P1 y ACoP, el 33 % de los CAC presentaron conformación fetal unilateral. Independientemente de la variabilidad que presenta el CAC, existe consenso de que la ACoP exhibe la mayor variabilidad. La comprensión de esta variabilidad requiere un análisis de los aspectos embrionarios que pueden explicar la conformación fetal de esté círculo arterial en el adulto.

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The description of the base of the human brain and its arteries that form a circle or polygon, as described and depicted by Thomas Willis and collaborators (1664), and that received his name ­ 'circle of Willis', has a long history, where many renowned preceding authors are included ­ the pre-Willisian anatomists, among which the names of Giulio Casserio (1627), Johann Vesling (1647) e Johann Jakob Wepfer (1658) deserve to be highlighted. However, despite a complete description and correct depiction of the arterial components of the circle, their naming lagged behind. After Willis, a large number of renowned authors ­ the post-Willisian anatomists, studied this formation further. This period begun with a poor contribution of Isbrand van Diemerbroeck (1672). Next appeared authors who provided names that became ephemeral, followed by those who presented designations that would remain permanently. Among the latter must be cited initially Joseph Lieutaud (1742) and Albrecht von Haller (1756), followed by Xavier Bichat with his posthumous work (1803), and finally the definitive names being established by Jean Cruveilhier (1834), this period closing with Henry Gray's book (1858), who consolidated the knowledge on the subject.

A descrição da base do cérebro humano e das artérias que formam um círculo ou polígono, como descrito e ilustrado por Thomas Willis e colaboradores (1664) e que recebeu seu nome - 'círculo de Willis', tem uma longa história, onde constam muitos autores de renome que o precederam ­ os anatomistas pré-Willisianos, entre os quais os nomes de Giulio Casserio (1627), Johann Vesling (1647) e Johann Jakob Wepfer (1658) merecem ser destacados. Entretanto, apesar da descrição completa e ilustração correta dos componentes arteriais do círculo, a denominação dos mesmos ficou atrasada. Após Willis, um grande número de autores renomados ­ os anatomistas pós-Willisianos, continuaram a estudar essa formação. Este período começou com uma contribuição pobre de Isbrand van Diemerbroeck (1672). A seguir apareceram autores que proveram nomes que se mostraram efêmeros, seguidos por aqueles que apresentaram designações que iriam permanecer de modo permanente. Entre os últimos devem ser citados inicialmente Joseph Lieutaud (1742) e Albrecht von Haller (1756), seguidos por Xavier Bichat com sua obra póstuma (1803), e finalmente, os nomes definitivos sendo estabelecidos por Jean Cruveilhier (1834), o período fechando com o livro de Henry Gray (1858), que consolidou o conhecimento sobre o tema.

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BACKGROUND AND PURPOSE: Information on the association between anatomical variants of the Circle of Willis (CoW) and intracranial atherosclerotic disease (ICAD) is limited and results are controversial. In this population-based study, we aimed to assess whether an incomplete CoW is associated with high calcium content in carotid siphons (a reliable biomarker of ICAD) in community-dwelling older adults of Amerindian ancestry. METHODS: Individuals aged ≥60 years enrolled in the Three Villages Study received a head computed tomography (CT) and magnetic resonance angiogram (MRA) of intracranial vessels. The CoW was classified in complete or incomplete according to the presence or absence of one A1 segment of the anterior cerebral artery or one or both P1 segments of posterior cerebral arteries. Calcium content in carotid siphons was rated as low or high. A multivariate logistic model was fitted to assess the independent association between incompleteness of the CoW and high calcium content in carotid siphons, after adjusting for demographics and cardiovascular risk factors. RESULTS: A total of 581 individuals were enrolled (mean age: 71 ± 8.4 years; 57% women). MRA revealed an incomplete CoW in 227 (39%) individuals, and high-resolution CT disclosed high calcium content in carotid siphons in 185 (32%). A risk factor logistic regression model showed no independent association between incompleteness of the CoW and high calcium content in carotid siphons (odds ratio: 0.91; 95% confidence interval: 0.62-1.34; p = 0.631). CONCLUSION: Study results disclosed no association between anatomical variants of the CoW and the presence of high calcium content in carotid siphons.

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SUMMARY: Fetal-type variant of the posterior cerebral artery is a relatively common variant of the cerebral arterial circle (circle of Willis), but concurrent cerebral pathologies have not been well reported. We describe a case of fetal-type variant of the posterior cerebral artery and concurrent bilateral cerebral infarctions in the territories of the middle cerebral artery in a 78-year-old Korean male cadaver. Fetal-type variant of the posterior cerebral artery was found the right cerebral arterial circle, arose from the internal carotid artery with larger diameter than the pre-communicating segment from the basilar artery. Histopathological examination revealed that left supramarginal gyrus and right infraparietal lobule showed characteristic cerebral infarctions with chronological changes, respectively. Knowledge on the variation in the posterior cerebral artery combined with clinical features including cerebral infarction plays a pivotal role to anatomists and clinicians.

RESUMEN: La variante de tipo fetal de la arteria cerebral posterior es una variante relativamente común del círculo arterial cerebral (polígono de Willis) de arterial cerebral, pero las patologías cerebrales concurrentes no han sido bien informadas. Describimos un caso de variante de tipo fetal de la arteria cerebral posterior e infartos cerebrales bilaterales concurrentes en los territorios de la arteria cerebral media en un cadáver masculino coreano de 78 años. La variante de tipo fetal de la arteria cerebral posterior se encontró en la parte de derecha del círculo arterial cerebral, surgido de la arteria carótida interna con mayor diámetro que el segmento precomunicante de la arteria basilar. El examen histopatológico reveló que el giro supramarginal izquierdo y el lóbulo infraparietal derecho mostraban infartos cerebrales característicos con cambios cronológicos, respectivamente. El conocimiento sobre la variación en la arteria cerebral posterior combinado con las características clínicas, incluido el infarto cerebral es fundamental para los anatomistas y los médicos.

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The description of arteries at the base of the human brain forming an 'arterial circle', named after Thomas Willis, has had a long history after the restoration of human dissection, partly due to the studies of many outstanding anatomists that preceded Willis. He provided, with the collaboration of Richard Lower and Christopher Wren, the first incontestable complete description, as recognized nowadays, accompanied by a superb illustration. Additionally, he presented an explanation for its meaning, indicating for the first time the functional significance of this structure, in health and disease. However, it should be recognized that the initial studies of the arteries of the base of the human brain by Willis' predecessors, as well as those from ancient times, despite their fragmentary descriptions, were certainly pivotal in paving the way for further and more detailed knowledge of this vascular formation.

A descrição das artérias da base do cérebro humano, formando um 'círculo arterial', designado com o nome de Thomas Willis, tem uma longa história após o restauro de dissecções humanas, em parte devido aos estudos de muitos anatomistas de renome que precederam Willis. Ele proveu, com a colaboração de Richard Lower e Christopher Wren, a primeira descrição completa e incontestável, assim como a reconhecida atualmente, acompanhada por uma ilustração soberba. Adicionalmente, apresentou uma explicação quanto ao seu significado, indicando pela primeira vez a importância funcional dessa estrutura, na saúde e na doença. Entretanto, deve ser reconhecido que os estudos iniciais das artérias da base do cérebro humano pelos predecessores de Willis, assim como os de tempos antigos, apesar de suas descrições fragmentárias, certamente foram fulcrais na pavimentação do caminho para o conhecimento mais avançado e detalhado dessa formação vascular.

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RESUMEN: El círculo arterial cerebral (CAC) mencionado también como polígono de Willis es una red de anastomosis vascular situado en la base del cerebro, constituido por ramas de la arteria carótida interna (ACI) y del sistema vertebro-basilar. Este estudio evaluó la morfología de las arterias que conforman el CAC en una muestra de individuos colombianos. Previa canalización de la ACI y de la arteria vertebral (AV), se perfundió con resina poliéster (palatal 85 % y estireno 15 %) los lechos vasculares del CAC de 70 encéfalos extraídos de cadáveres a quienes se les practicó necropsia en el Instituto de Medicina Legal de Bucaramanga, Colombia. La arteria comunicante anterior (ACoA) se observó en 68 encéfalos (97,1 %), con ausencia en 2 de las muestras (2,9 %); en promedio su diámetro fue de 1,91?1,04 mm y su longitud 2,21?0,97 mm respectivamente. Asimismo, se encontró hipoplasia en 6 muestras (8,4 %). La arteria comunicante posterior (ACoP) estuvo ausente en 2 de las muestras (5,7 %); su longitud fue 11,63?2,12 mm, mientras que su calibre fue de 1,21?0,58 mm, siendo ligeramente mayor en el lado derecho, sin diferencias estadísticamente significativas con relación al lado de presentación (p= 0,763). Se encontró hipoplasia de la ACoP en forma bilateral en 19 de las muestras (27,1 %) y unilateral en 15 muestras (21,4 %). En 8 muestras (20 %) de 35 CAC evaluados se observó configuración fetal. La incidencia de hipoplasia de la ACoP y de configuración fetal encontrados en el presente estudio, se ubican en el segmento superior de lo reportado en la literatura. Estas expresiones morfológicas han sido consideradas como coadyuvantes en el desarrollo de accidentes cerebro-vasculares (ACV).

SUMMARY: The cerebral arterial circle (CAC), also referred to as the polygon of Willis is a network of vascular anastomoses located at the base of the brain, consisting of branches of the internal carotid artery (ICA) and the vertebrobasilar system (VBS). This study evaluated the morphology of the arteries forming the CAC in a sample of Colombian individuals. After cannulation of the ICA and the vertebral artery (VA), the vascular beds of the ACC of 70 brains extracted from cadavers at the Institute of Legal Medicine of Bucaramanga, Colombia, were perfused with polyester resin (85 % palatal and 15 % styrene). The anterior communicating artery (ACoA) was observed in 68 brains (97.1 %), with absence in 2 of the samples (2.9 %); on average its diameter was 1.91?1.04mm and its length 2.21?0.97mm respectively. Likewise, hypoplasia was found in 6 samples (8.4 %). The posterior communicating artery (ACoP) was absent in 2 of the samples (5,7 %); its length was 11.63?2.12mm, while its caliber was 1.21?0.58mm, being slightly larger on the right side, with no statistically significant differences in relation to the side of presentation (p= 0.763). Hypoplasia of the ACoP was found bilaterally in 19 of the samples (27.1 %) and unilaterally in 15 samples (21.4 %). Fetal configuration was observed in 8 samples (20 %) of 35 CACs evaluated. The incidence of ACoP hypoplasia and fetal configuration found in the present study are in the upper segment of those reported in the literature. These morphological expressions have been considered as coadjuvants in the development of cerebrovascular accidents (CVA).

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BACKGROUND: Papillomaviruses are small nonenveloped, circular double-stranded DNA viruses that belong to the Papillomaviridae family. To date, 29 Bos taurus papillomavirus (BPV) types have been described. Studies involving mixed BPV infections have rarely been reported in contrast to human papillomavirus (HPV), which is commonly described in numerous studies showing coinfections. Moreover, previous studies had shown that HPV coinfections increase the risk of carcinogenesis. In the present study, we used rolling-circle amplification followed by a high-throughput sequencing (RCA-HTS) approach in 23 teat papillomas from southern Brazil. RESULTS: Eleven well-characterized BPV types and 14 putative new BPV types were genetically characterized into the Xi, Epsilon and Dyoxipapillomavirus genera according to phylogenetic analysis of the L1 gene, which expands the previous 29 BPV types to 43. Moreover, BPV coinfections were detected in the majority (56.3%) of the papilloma lesions analyzed, suggesting a genetic diverse "papillomavirome" in bovine teat warts. CONCLUSIONS: The data generated in this study support the possibility that a wide range of BPV is probably underdetected by conventional molecular detection tools, and that BPV coinfections are underestimated and probably genetic diverse. Additionally, 14 new BPV types were characterized, increasing the knowledge regarding BPV genetic diversity.

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Introduction: "Blue Economy" refers to ocean-based economies with a sustainable approach. It focuses in smaller carbon footprints and efficiency, principles that can be applied to aquaculture. However, it has been difficult to develop successful blue economy projects in sea urchin aquaculture. Objective: To compare URCHINOMICS (Norway) and ARBACIA (Argentina), two aquaculture projects with different business models. Methods: We used publicly available information to compare both companies on the basis of their value proposition and tensions (e.g. cultural, social economic and technological). Results: To be successful, sea urchin aquaculture requires development of appropriate technology, open innovation and cooperation of people with different academic, business and organizational backgrounds. Conclusion: The ultimate success of these and similar companies will depend on free interaction of experts from multiple fields and on technological innovation.

Introducción: "Economía azul" se refiere a las economías basadas en el océano con un enfoque sostenible. Se enfoca en menores huellas de carbono y eficiencia, principios que se pueden aplicar a la acuicultura. Sin embargo, ha sido difícil desarrollar proyectos exitosos de economía azul en la acuicultura de erizos de mar. Objetivo: Comparar URCHINOMICS (Noruega) y ARBACIA (Argentina), dos proyectos de acuicultura con diferentes modelos de negocio. Métodos: Utilizamos información disponible públicamente para comparar ambas empresas sobre la base de su propuesta de valor y tensiones (por ejemplo, culturales, socioeconómicas y tecnológicas). Resultados: Para tener éxito, la acuicultura de erizos de mar requiere el desarrollo de tecnología adecuada, innovación abierta y cooperación de personas con diferentes antecedentes académicos, empresariales y organizativos. Conclusión: El éxito final de estas y otras empresas similares dependerá de la libre interacción de expertos de múltiples campos y de la innovación tecnológica.

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INTRODUCCIÓN: En la actualidad el ictus es considerado una de las principales causas de discapacidad en el mundo. Globalmente 5 millones de personas adquieren discapacidad permanente cada año por esta causa; hasta el 30% de los pacientes afectados padecen algún tipo de discapacidad. El manejo temprano del paciente puede disminuir las secuelas derivadas de las lesiones. El objetivo de este estudio es conocer si existen diferencias en las discapacidades derivadas de ictus entre las ciudades de Madrid (España) y Cuenca (Ecuador), en relación a una atención temprana programada. MATERIALES Y MÉTODOS: El presente, es un estudio observacional, descriptivo, de corte transversal, multicéntrico. La muestra estuvo conformada por 40 pacientes diagnosticados de ACV, 20 pertenecientes al Grupo Cuencano, de Ecuador (GC) y 20 al Grupo Matritense, de España (GM). Los datos fueron obtenidos de las historias clínicas. Se compararon ambos grupos mediante la prueba Chi-cuadrado (X2) de Pearson para cada variable estudiada. RESULTADOS: En el GM el 80% de los ACV fueron de origen isquémico, en el GC fueron el 90%, sin asociación significativa. En los dos grupos el mayor porcentaje de secuelas se dieron por afectación del territorio de la arteria cerebral media (ACM). No se evidenció diferencia significativa en el porcentaje de discapacidades derivadas de ictus entre los grupos, el grupo matritense de España presentó una menor tasa de discapacidades derivadas que fue del 45%, frente al grupo cuencano de Ecuador que tuvo un 76% de capacidades derivadas, pero la diferencia no fue significativa (p=0.069); sin embargo la asociación entre el porcentaje de exitus entre los grupos si tuvo asociación estadísticamente significativa (p=0.003). CONCLUSIÓN: No se encontró asociación significativa en cuanto a etiología, factores de riesgos y características clínicas del Ictus entre los grupos. No hay diferencias significativas en las secuelas derivadas de ictus entre los grupos, pero si hay diferencias significativas en relación al porcentaje de exitus con la aplicación de un programa tipo "Código Ictus".

BACKGROUND: Currently, stroke is one of the main causes of disability in the world. Globally 5 million people acquire permanent disability each year for this cause; up to 30% of affected patients suffer from some type of disability. Early management of the patient can reduce the sequelae derived from the injury. The aim of this study is to find out if there are differences in disabilities derived from strokes, between the cities of Madrid (Spain) and Cuenca (Ecuador), in association with programmed early patient care. METHODS: This is an observational, descriptive, cross-sectional, multicenter study. The sample consisted of 40 patients diagnosed with stroke, 20 from Cuenca, Ecuador, and 20 from Madrid, Spain. Data was obtained from the patient's medical records. Both groups were compared using Pearson's Chi-square test (Χ2) for each studied variable. RESULTS: in the Madrid Group 80% of the strokes were ischemic, in the Cuenca Group 90% of the strokes were ischemic, there is not significant association. In both groups the highest percentage of sequelae occurred due to injury of the middle cerebral artery (MCA). There was no significant difference in the percentage of disabilities derived from stroke between the groups, the Madrid Group had a lower rate of derived disabilities (45%), compared to Cuenca Group (76%), but the differences was not significant (p=0.069); however, the association between the death percentage among the groups was statistically significant (p=0.003). CONCLUSION: No significant association was found in terms of etiology, risk factors and clinical characteristics of stroke between the groups. There are no significant differences in the sequelae derived from stroke between the groups, but there is significant difference in terms of death percentage between the groups, with the application of "Código Ictus" type of program.

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ABSTRACT Objectives: to share the contributions of culture circles for teaching, research, and professional nursing practice, having as framework Paulo Freire's Research Itinerary. Methods: this is an experience report of a culture circle, with participation of three professors and ten students, enrolled in a course of a Graduate Program in Nursing in southern Brazil. In this culture circle, a tree was built in which the roots formed the thematic investigation, the stem, coding and decoding, and the leaves, the Research Itinerary critical unveiling. Results: participants demonstrated empowerment of Paulo Freire's assumptions, and building a tree made it possible to discuss in a pleasant and playful way culture circle use in teaching, research, and professional nursing practice. Final Considerations: the culture circle promoted reflection and action on nursing praxis, turning Freirean thoughts into something concrete and transforming realities.

RESUMEN Objetivos: compartir acerca de las contribuciones del círculo cultural a la docencia, la investigación y la práctica profesional de la enfermería, tomando como referencia el Itinerario de Investigación Freireano. Métodos: relato de experiencia de un círculo cultural, con la participación de tres profesores y diez estudiantes, matriculados en una disciplina de un Posgrado en Enfermería en el sur de Brasil. En el círculo cultural se construyó un árbol en el que las raíces formaron la investigación temática, el tallo, codificación y decodificación, y las hojas, la develación crítica del Itinerario de Investigación. Resultados: los participantes demostraron empoderamiento de los supuestos freireanos, y la construcción del árbol permitió discutir, de manera amena y lúdica, el uso del círculo cultural en la docencia, la investigación y la práctica profesional de enfermería. Consideraciones Finales: el círculo cultural promovió la reflexión y la acción sobre la praxis de la enfermería, convirtiendo el pensamiento freireano en algo concreto y transformando realidades.

RESUMO Objetivos: compartilhar sobre as contribuições do círculo de cultura para o ensino, pesquisa e prática profissional da enfermagem, tendo como referencial o Itinerário de Pesquisa freireano. Métodos: relato de experiência de um círculo de cultura, com a participação de três docentes e dez discentes, matriculados em uma disciplina de um Programa de Pós-Graduação em Enfermagem no sul do Brasil. No círculo de cultura, foi construída uma árvore em que as raízes formaram a investigação temática, o caule, codificação e decodificação, e as folhas, o desvelamento crítico do Itinerário de Pesquisa. Resultados: os participantes demonstraram empoderamento dos pressupostos freireanos, e a construção da árvore possibilitou discutir, de maneira prazerosa e lúdica, a utilização do círculo de cultura no ensino, pesquisa e prática profissional da enfermagem. Considerações Finais: o círculo de cultura promoveu reflexão e ação sobre a práxis da enfermagem, tornando os pensamentos freireanos em algo concreto e transformador de realidades.

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