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Molecular typing of bovine papillomaviruses in Costa Rica.
Alfaro-Mora, Ramsés; Castro-Vásquez, Ruth; Zobba, Rosanna; Antuofermo, Elisabetta; Burrai, Giovanni Pietro; Alberti, Alberto; Dolz, Gaby.
Afiliação
  • Alfaro-Mora R; Maestría en Enfermedades Tropicales, Posgrado Regional en Ciencias Veterinarias Tropicales, Universidad Nacional, Campus Benjamín Núñez, Barreal de Heredia, Costa Rica. ramses.alfaro@ulatina.net.
  • Castro-Vásquez R; Dirección de Investigación, Universidad Latina de Costa Rica, Campus San Pedro, San José, Costa Rica. ramses.alfaro@ulatina.net.
  • Zobba R; Maestría en Enfermedades Tropicales, Posgrado Regional en Ciencias Veterinarias Tropicales, Universidad Nacional, Campus Benjamín Núñez, Barreal de Heredia, Costa Rica.
  • Antuofermo E; Laboratorio de Biología Molecular, Instituto de Innovación y Transferencia en Tecnología Agropecuaria, San José, Costa Rica.
  • Burrai GP; Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy.
  • Alberti A; Mediterranean Center for Disease Control, University of Sassari, Via Vienna 2, 07100, Sassari, Italy.
  • Dolz G; Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy.
Vet Res Commun ; 47(1): 265-272, 2023 Jan.
Article em En | MEDLINE | ID: mdl-35303259
Bovine papillomaviruses are related to cause fibroepithelial proliferations in the skin and mucosae and are associated with economic loss mainly related to poor body condition and reduced milk production. This study aimed to investigate the presence and types of bovine papillomaviruses (BPVs) in cattle sampled in different areas of Costa Rica using molecular techniques. A descriptive study with a non-probability convenience sampling was carried out. A total of 99 papillomatous lesions were collected from 63 animals in 32 farms, and analyzed by polymerase chain reaction, rolling circle amplification (RCA), sequencing, and restriction enzymes digestion. Seven bovine papillomavirus types (BPV1, BPV2, BPV4, BPV6, BPV7, BPV10, BPV11) and two putative novel viral variants (BPV-CR1 and BPV-CR2) were identified for the first time in Costa Rica. BPV6 was the most frequently detected virus in lesions (31.2%), followed by BPV2 (25%) and BPV1 (25%). BPV1 and BPV2 were the most widely distributed in the Country. Coinfections were recorded in two animals (BPV1 / BPV2 and BPV4 / BPV6). Restriction analyses allowed differentiating BPV1 from BPV2, BPV4, and BPV7, but failed to identify BPV6, BPV10, and BPV11. Results suggest that a great PVs diversity is harbored by bovines in Costa Rica and indicate the need for further investigations aimed to uncover PV diversity at the full genomic level.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Papillomavirus Bovino 1 Limite: Animals País/Região como assunto: America central / Costa rica Idioma: En Revista: Vet Res Commun Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Papillomavirus Bovino 1 Limite: Animals País/Região como assunto: America central / Costa rica Idioma: En Revista: Vet Res Commun Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Suíça