RESUMO
ABSTRACT Caligo teucer (Linnaeus, 1758) is widely distributed in Argentina, Bolivia, Brazil and Ecuador. The objective was to study biological aspects of Caligo teucer japetus Stichel, 1903 with banana leaves, Musa sapientium L. (Zingiberales: Musaceae), in the butterfly garden, under environmental conditions, and in the laboratory of biological control of insects at the Universidade Federal de Viçosa in Viçosa, Minas Gerais state, Brazil at 24 ± 2 oC, 68 ± 10% relative humidity and 12 hours photophase. The duration of the egg incubation, larvae, pupa and egg to adult periods of C. teucer japetus were, respectively, 11.8 ± 0.1; 53.9 ± 0.9; 17.9 ± 0.3 and 82.6 ± 1.0 days for females, and 11.8 ± 0.1; 50.3 ± 0.6; 18.4 ± 0.3 and 79.4 ± 0.6 days for males in cages in the laboratory. The longevity of C. teucer japetus adults was 26.0 ± 10.4 and 47.5 ± 8.7 for females and 24.7 ± 3.5 and 35.4 ± 15.7 for males in the butterfly garden and in laboratory cages, respectively. The high survival and the relatively short period of development of its immature stages confirm that banana leaves are an adequate food substrate for the development and survival of Caligo teucer japetus.
RESUMO
This study was carried out to evaluate the in vitro effect of Musa x paradisiaca stem and leaf against the parasitic nematode of small ruminants Haemonchus contortus. Three extracts (aqueous, methanolic and/or dichloromethane) of Musa x paradisiaca stem and leaf were tested in vitro on four developmental stages of H. contortus using egg hatch assay (EHA), larval development assay (LDA), L3 migration inhibition assay (LMI) and adult worm motility assay (AWM). The highly significant (P<0.0001) ability to stop larval development (inhibition >67% for each extract) and the negative effect of the dichloromethane extract of leaf on adult worm motility (43% of inhibition of motility after 24h of incubation) compared to the negative controls, suggest anthelmintic properties of Musa x paradisiaca stem and leaf against H. contortus. The active principles responsible for the activity could be secondary metabolites such as terpenoid and flavonoid compounds present in the leaf and stem of the plant.