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1.
Infect Genet Evol ; 97: 105181, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34896287

RESUMO

Although Bartonella spp. is described in cats worldwide, little is known about the occurrence and genetic diversity of Bartonella spp. in cats from South America. To date, it has only been detected in cats from Brazil, Chile and Argentina. This study aimed to undertake a molecular survey and explore the genetic diversity of Bartonella spp. in domestic cats from Paraguay. A TaqMan real-time quantitative PCR (qPCR) targeting the nuoG gene (83 bp) for Bartonella spp. was used to screen 125 blood samples from cats in Asuncion, Paraguay. nuoG qPCR-positive samples were further submitted to conventional PCR assays based on the ITS (453- 717 bp), gltA (767 bp), ftsZ (515 bp), rpoB (333 bp), ribC (585-588 bp), and pap-31 (564 bp) loci. Positive samples were sequenced for species identification, phylogenetic, and haplotype analyses. Bartonella D.N.A. was present in 20.8% (26/125) cat blood samples, with low levels of Bartonella nuoG D.N.A. cPCR products targeting gltA, ftsZ, ITS, and rpoB loci from sixteen cats were successfully sequenced. However, all nouG qPCR-positive samples were negative for the ribC and pap-31 genes. Bartonella henselae [62.5% (10/16)] and Bartonella clarridgeiae [37.5% (6/16)] were identified among the sequenced samples. Upon phylogenetic analysis, B. henselae and B. clarridgeiae from Paraguay clustered with sequences detected in domestic and wild cats, dogs, and cat fleas worldwide. Two to four haplotypes of B. henselae and B. clarridgeiae in cats from Paraguay were observed, with some being exclusive and others shared with worldwide distributed haplotypes. Here, we report B. henselae and B. clarridgeiae for the first time in cats from Paraguay. Its circulation in cats suggests the need to consider Bartonellae when testing clinical samples from suspected infectious diseases in humans from Paraguay.


Assuntos
Infecções por Bartonella/veterinária , Bartonella henselae/genética , Bartonella/genética , Doenças do Gato/epidemiologia , Variação Genética , Animais , Infecções por Bartonella/epidemiologia , Gatos , Paraguai/epidemiologia , Filogenia
2.
J Vector Ecol ; 40(2): 327-32, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26611968

RESUMO

Cats and their fleas collected in Guatemala were investigated for the presence of Bartonella infections. Bartonella bacteria were cultured from 8.2% (13/159) of cats, and all cultures were identified as B. henselae. Molecular analysis allowed detection of Bartonella DNA in 33.8% (48/142) of cats and in 22.4% (34/152) of cat fleas using gltA, nuoG, and 16S-23S internal transcribed spacer targets. Two Bartonella species, B. henselae and B. clarridgeiae, were identified in cats and cat fleas by molecular analysis, with B. henselae being more common than B. clarridgeiae in the cats (68.1%; 32/47 vs 31.9%; 15/47). The nuoG was found to be less sensitive for detecting B. clarridgeiae compared with other molecular targets and could detect only two of the 15 B. clarridgeiae-infected cats. No significant differences were observed for prevalence between male and female cats and between different age groups. No evident association was observed between the presence of Bartonella species in cats and in their fleas.


Assuntos
Infecções por Bartonella/veterinária , Bartonella , Doenças do Gato/microbiologia , Ctenocephalides/microbiologia , Animais , Bartonella/genética , Bartonella/isolamento & purificação , Bartonella/patogenicidade , Bartonella/fisiologia , Infecções por Bartonella/epidemiologia , Infecções por Bartonella/microbiologia , Bartonella henselae/genética , Bartonella henselae/patogenicidade , Bartonella henselae/fisiologia , Doenças do Gato/epidemiologia , Gatos , Feminino , Infestações por Pulgas/epidemiologia , Guatemala/epidemiologia , Masculino , RNA Ribossômico 16S
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