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Molecular survey and genetic diversity of Bartonella spp. in domestic cats from Paraguay.
Sepúlveda-García, Paulina; Pérez-Macchi, Sandra; Gonçalves, Luiz Ricardo; do Amaral, Renan Bressianini; Bittencourt, Pedro; André, Marcos Rogério; Muller, Ananda.
Afiliação
  • Sepúlveda-García P; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile; Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
  • Pérez-Macchi S; Departamento de Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Nacional de Asunción, 001109, Paraguay.
  • Gonçalves LR; Departamento de Patologia, Reprodução e Saúde Única, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, São Paulo 14884-900, Brazil.
  • do Amaral RB; Departamento de Patologia, Reprodução e Saúde Única, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, São Paulo 14884-900, Brazil.
  • Bittencourt P; One Health Center for Zoonoses and Tropical Veterinary Medicine, Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
  • André MR; Departamento de Patologia, Reprodução e Saúde Única, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, São Paulo 14884-900, Brazil.
  • Muller A; One Health Center for Zoonoses and Tropical Veterinary Medicine, Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis; Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia
Infect Genet Evol ; 97: 105181, 2022 01.
Article em En | MEDLINE | ID: mdl-34896287
Although Bartonella spp. is described in cats worldwide, little is known about the occurrence and genetic diversity of Bartonella spp. in cats from South America. To date, it has only been detected in cats from Brazil, Chile and Argentina. This study aimed to undertake a molecular survey and explore the genetic diversity of Bartonella spp. in domestic cats from Paraguay. A TaqMan real-time quantitative PCR (qPCR) targeting the nuoG gene (83 bp) for Bartonella spp. was used to screen 125 blood samples from cats in Asuncion, Paraguay. nuoG qPCR-positive samples were further submitted to conventional PCR assays based on the ITS (453- 717 bp), gltA (767 bp), ftsZ (515 bp), rpoB (333 bp), ribC (585-588 bp), and pap-31 (564 bp) loci. Positive samples were sequenced for species identification, phylogenetic, and haplotype analyses. Bartonella D.N.A. was present in 20.8% (26/125) cat blood samples, with low levels of Bartonella nuoG D.N.A. cPCR products targeting gltA, ftsZ, ITS, and rpoB loci from sixteen cats were successfully sequenced. However, all nouG qPCR-positive samples were negative for the ribC and pap-31 genes. Bartonella henselae [62.5% (10/16)] and Bartonella clarridgeiae [37.5% (6/16)] were identified among the sequenced samples. Upon phylogenetic analysis, B. henselae and B. clarridgeiae from Paraguay clustered with sequences detected in domestic and wild cats, dogs, and cat fleas worldwide. Two to four haplotypes of B. henselae and B. clarridgeiae in cats from Paraguay were observed, with some being exclusive and others shared with worldwide distributed haplotypes. Here, we report B. henselae and B. clarridgeiae for the first time in cats from Paraguay. Its circulation in cats suggests the need to consider Bartonellae when testing clinical samples from suspected infectious diseases in humans from Paraguay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bartonella / Infecções por Bartonella / Variação Genética / Doenças do Gato / Bartonella henselae Limite: Animals País/Região como assunto: America do sul / Paraguay Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bartonella / Infecções por Bartonella / Variação Genética / Doenças do Gato / Bartonella henselae Limite: Animals País/Região como assunto: America do sul / Paraguay Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Holanda