RESUMO

Inhibitor of nuclear factor κB kinase 2 (IKK2) is suggested to be a potential target for the development of novel anti-inflammatory and anticancer drugs. In this work, we applied structure-based drug design to improve the potency of the inhibitor (E)-N'-(4-nitrobenzylidene)-2-naphthohydrazide (LASSBio-1524, 1 a: IC50 =20 µm). The molecular model built for IKK2 together with the docking methodology employed were able to provide important and consistent information with respect to the structural and chemical inhibitor characteristics that may confer potency to IKK2 inhibitors, providing important guidelines for the development of a new N-acylhydrazone (NAH) derivative. (E)-N'-(4-(1H-pyrrolo[2,3-b]pyridin-4-yl)benzylidene)-2-naphthohydrazide hydrochloride (LASSBio-1829 hydrochloride, 10) is a 7-azaindole NAH able to inhibit IKK2 with an IC50 value of 3.8 µm. LASSBio-1829 hydrochloride was found to be active in several pharmacological inflammation tests in vivo, showing its potential as an anti-inflammatory prototype.

Assuntos

Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/farmacologia , Compostos de Benzilideno/administração & dosagem , Compostos de Benzilideno/farmacologia , Quinase I-kappa B/antagonistas & inibidores , Naftalenos/administração & dosagem , Naftalenos/farmacologia , Inibidores de Proteínas Quinases/administração & dosagem , Inibidores de Proteínas Quinases/farmacologia , Administração Oral , Anti-Inflamatórios não Esteroides/química , Compostos de Benzilideno/química , Relação Dose-Resposta a Droga , Desenho de Fármacos , Humanos , Quinase I-kappa B/metabolismo , Modelos Moleculares , Estrutura Molecular , Naftalenos/química , Inibidores de Proteínas Quinases/química , Relação Estrutura-Atividade