RESUMO
Common bean has the potential to improve gut microbiota function due to its chemical composition and content of dietary fiber. This study evaluated the effect of cooked common bean (CCB) flour and its protein hydrolysate as part of a high-fat diet (HFD) added with 6-propyl-2-thiouracil (10 mg/kg/d), an inhibitor of thyroid hormone synthesis, on gut health of BALB/c mice. Forty-eight adult mice were divided into four groups: normal control; HFD; HFD plus CCB flour (346.6 g/kg of diet) (HFBF group) and HFD plus CCB protein hydrolysate (700 mg/Kg/d) (HFPH group). HFBF, but not HFPH, increased cecum weight, and the moisture, and lipids in the excreted feces, compared to control groups. Sequencing of the 16S rRNA gene of the cecal microbiota indicated changes in the beta-diversity between the HFBF and HFPH groups, compared to the normal control. The abundance of Bacteroidetes increased and the Firmicutes/Bacteroidetes ratio decreased in the HFBF compared to control groups. However, HFPH was not able to prevent the damage caused by a HFD to the gut bacterial communities. The OTUs enriched by HFBF were mainly assigned to members of the Muribaculaceae family, which shows potential to improve gut health. The intake of CCB flour improved intestinal health and modulated the composition and function of the cecal microbiota, attenuating the effects of the HFD, added wit 6-propyl-2-thiouracil, when fed to BALB/c mice.