RESUMO

A procedure for the purification of a Peruvian isolate (C1) of sweet potato feathery mottle potyvirus (SPFMV) and infective RNA has been developed. The use of Hepes [N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid] buffer containing urea and sodium EDTA as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from Nicotiana benthamiana L. Domin. A short RNA isolation procedure yielded infectious RNA, from which ds cDNA of nearly genome size could be obtained. Sweet potato feathery mottle potyvirus, Purification, RNA isolation, cDNA synthesis.

Assuntos

Nicotiana/microbiologia , Plantas Tóxicas , Potyvirus/isolamento & purificação , RNA Viral/isolamento & purificação , Vírion/isolamento & purificação , Soluções Tampão , DNA Complementar/biossíntese , Ácido Edético , HEPES , Extratos Vegetais , Potyvirus/crescimento & desenvolvimento , Potyvirus/patogenicidade , Potyvirus/ultraestrutura , Ureia , Verduras/microbiologia , Vírion/ultraestrutura , Cultura de Vírus