Sweet potato feathery mottle potyvirus (C1 isolate) virion and RNA purification.
J Virol Methods
; 44(1): 109-16, 1993 Sep.
Article
em En
| MEDLINE
| ID: mdl-8227274
A procedure for the purification of a Peruvian isolate (C1) of sweet potato feathery mottle potyvirus (SPFMV) and infective RNA has been developed. The use of Hepes [N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid] buffer containing urea and sodium EDTA as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from Nicotiana benthamiana L. Domin. A short RNA isolation procedure yielded infectious RNA, from which ds cDNA of nearly genome size could be obtained. Sweet potato feathery mottle potyvirus, Purification, RNA isolation, cDNA synthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Nicotiana
/
Vírion
/
RNA Viral
/
Potyvirus
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Peru
País de publicação:
Holanda