RESUMO
The aim of the present study was to develop an in vitro test model from human teeth to comparatively examine antibacterial effectiveness of calcium hydroxide, parachlorophenol (PCP) and camphorated parachlorophenol (CPCP) against Enterococcus faecalis in infected root canals. Cylindrical dentin specimens were prepared from freshly extracted human maxillary anterior teeth. The specimens were inoculated with E. faecalis and then medicated with either CPCP, PCP or Ca(OH)2. The disinfecting efficacy of these agents was tested by collecting dentin chips from the inner ("canal") walls of the specimens and counting viable E. faecalis. The dentin chips were diluted and a classical bacterial count technique was used for recovery of E. faecalis strains of 5% sheep blood agar. The effectiveness of CPCP and PCP at one day was superior to the effectiveness of Ca(OH)2. In the three-day group, CPCP was the most effective, followed by Ca(OH)2. The experimental model used in this study may be useful for investigation of the effect of intracanal medicaments on microorganisms lodged in the root dentinal tubules.
Assuntos
Desinfetantes de Equipamento Odontológico/farmacologia , Dentina/efeitos dos fármacos , Dentina/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Infecções por Bactérias Gram-Positivas/microbiologia , Hidróxido de Cálcio/farmacologia , Cânfora/farmacologia , Clorofenóis/farmacologia , Contagem de Colônia Microbiana , Cavidade Pulpar/microbiologia , Combinação de Medicamentos , Enterococcus faecalis/crescimento & desenvolvimento , Humanos , Técnicas In Vitro , Testes de Sensibilidade Microbiana , Irrigantes do Canal Radicular/farmacologia , Fatores de TempoRESUMO
The aim of this study was to evaluate, in vitro, the early tensile bond strength values of three dentin bonding agents (Scotchbond, Tenure and Gluma) to dentin. Fifteen non-carious, extracted, human third molars were used in this study. The bond strengths were calculated in an Instron test machine and expressed in MN/m2. The results were evaluated statistically using the Student-test. The in vitro tensile bond strengths of the three bonding agents to dentin showed higher values than the tensile bond strength of composite resin alone and differences among the bond strengths were significant (P < 0.05). Greater bond strength values were obtained with the Gluma bonding system than with Scotchbond or Tenure systems.