An in vitro test model for investigation of disinfection of dentinal tubules infected with Enterococcus faecalis.
Braz Dent J
; 8(2): 67-72, 1997.
Article
em En
| MEDLINE
| ID: mdl-9590928
The aim of the present study was to develop an in vitro test model from human teeth to comparatively examine antibacterial effectiveness of calcium hydroxide, parachlorophenol (PCP) and camphorated parachlorophenol (CPCP) against Enterococcus faecalis in infected root canals. Cylindrical dentin specimens were prepared from freshly extracted human maxillary anterior teeth. The specimens were inoculated with E. faecalis and then medicated with either CPCP, PCP or Ca(OH)2. The disinfecting efficacy of these agents was tested by collecting dentin chips from the inner ("canal") walls of the specimens and counting viable E. faecalis. The dentin chips were diluted and a classical bacterial count technique was used for recovery of E. faecalis strains of 5% sheep blood agar. The effectiveness of CPCP and PCP at one day was superior to the effectiveness of Ca(OH)2. In the three-day group, CPCP was the most effective, followed by Ca(OH)2. The experimental model used in this study may be useful for investigation of the effect of intracanal medicaments on microorganisms lodged in the root dentinal tubules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Bactérias Gram-Positivas
/
Enterococcus faecalis
/
Desinfetantes de Equipamento Odontológico
/
Dentina
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Braz Dent J
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de publicação:
Brasil