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Coronavirus disease (COVID-19) is caused by infection with the SARS-CoV-2 virus, having already caused more than seven million deaths worldwide. Conventional techniques for SARS-CoV-2 detection have limitations, as high cost, low specificity, and longer analysis time, among others. Biosensors emerge as a necessary alternative to overcome the difficulties of current diagnostics. This paper reports a sensor platform where silver-doped zinc oxide nanomaterial (Ag:ZnONp) was used onto carbon screen-printed electrode and ethidium bromide as indicator for development of a specific electrochemical genosensor for COVID-19. This genosensor demonstrated good linearity between the concentrations of 5.62â¯×â¯104-5.62 copies/mL and a detection limit of 5 copies/mL with gRNA in patient's samples, with a response time within 30â¯min. Molecular modeling and morphological analysis are in agreement with obtained electrochemical results. Additional techniques such as AFM, SEM, and EIS were conducted to characterize the morphological and electrochemical properties of the biosensor's surface. The biosensor was also capable of detecting the target presence in spiked samples and demonstrated a stability of 60â¯days, higher than other similar biosensors for SARS-CoV-2.
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OBJECTIVE: This study aimed to develop a Digital Speech Assessment Instrument to evaluate the phonological and motor speech skills of Brazilian Portuguese-speaking children and adolescents through six tasks: (a) word naming, (b) word imitation, (c) pseudoword imitation, (d) word repetition, (e) pseudoword repetition, and (f) diadochokinesis. METHOD: The assessment instrument was developed and validated in a seven-step process, beginning with stimulus selection and ending with Pilot Study 1 (involving children and adolescents with typical speech development) and Pilot Study 2 (involving children and adolescents with speech sound disorders [SSDs]). Participants of the study included expert and nonexpert judges, children, and adolescents. RESULTS: The Digital Speech Assessment Instrument contains 91 real words (61 in the naming task, 51 in the imitation task, and five in the repetition task), 26 pseudowords (26 in the imitation task and four in the repetition task), and six stimuli for diadochokinesis. The test contains stimuli in the form of images as well as audio and video recordings and allows for the analysis and storage of participant data in a virtual database. CONCLUSIONS: This study described the development of the Digital Speech Assessment Instrument, available in Brazilian Portuguese for the evaluation of several aspects of speech production (including word and pseudoword naming, imitation, and repetition and diadochokinesis). The test was developed for children aged 2;0 (years;months) to 17;11 and is administered fully online. In the future, the instrument can be used to provide a timely and accurate diagnosis of SSDs.
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Medida da Produção da Fala , Transtorno Fonológico , Humanos , Criança , Adolescente , Masculino , Feminino , Transtorno Fonológico/diagnóstico , Projetos Piloto , Brasil , Reprodutibilidade dos Testes , Fonética , Pré-Escolar , Linguagem Infantil , Valor Preditivo dos TestesRESUMO
PURPOSE: To carry out the cross-cultural adaptation of the Instrument Profiles of Early Expressive Phonological Skills- Brazilian Portuguese (PEEPS-BP) - Expanded list, performing content validation. METHODS: Cross-cultural, quantitative and cross- sectional adaptation study, considering psychometric criteria. A study was carried out on the list of 423 words from the Communicative Development Inventory - MacArthur - Words and Sentences, adapted to Brazilian Portuguese. The method was divided into four steps. The list was judged by expert judges (JE) and non-specialist judges (JNE), considering as a criterion the familiarity of the word for a child aged between 24 and 36 months, and the representativeness of the word with toy/object, contemplating Steps 1 and 2. The child judges analyzed, in a data collection situation, the familiarity and representativeness of the stimulus-words, presented in Step 3. Afterwards, the pilot study - Step 4, was carried out with the selected stimulus-words. In the statistical analysis by expert and non-specialist judges, the Fleiss' Kappa and Gwet Concordance index was used. In the analysis of the responses of the child judges and in the Pilot Study, the analysis was made in relation to the type of response of the child, specifically scoring the spontaneous naming of the toy/object, scoring qualitatively. RESULTS: The result of Steps 1 and 2, and the agreement of the statistical tests for the Familiarity and Representativeness criteria was 45.7% for JE and 76.4% for JNE, and a result of 100% for the agreement of Representativeness. A total of 122 words were analyzed, resulting from previously established criteria, totaling 34 words (exclusion of one word by the researchers), totaling 33 stimulus-words. In Stage 3, of the 33 stimulus-words applied, nine presented spontaneous naming scores below expectations, being retested for Step 4, the Pilot Study. The result of the Pilot Study showed that of the nine retested stimulus-words, four of them still had a score below, being excluded from the study. Therefore, with the application of the Pilot Study, the expanded list of PEEPS-BP resulted in 29 words. CONCLUSION: The PEEPS-BP - Expanded List showed satisfactory evidence of content validity for the cross-cultural adaptation of the test.
OBJETIVO: Realizar a adaptação transcultural do Instrumento Profiles of Early Expressive Phonological Skills- Brazilian Portuguese (PEEPS-BP) - Lista expandida, realizando a validação de conteúdo. MÉTODO: Estudo de adaptação transcultural, quantitativo e transversal, considerando critérios psicométricos. Realizou-se o estudo da lista de 423 palavras do Inventário de Desenvolvimento Comunicativo- MacArthur- Palavras e Sentenças, adaptado para o Português Brasileiro. O método foi dividido em quatro Etapas. A lista foi julgada por juízes especialistas (JE) e juízes não especialistas (JNE), considerando como critério a familiaridade da palavra para uma criança entre 24 e 36 meses, e a representatividade da palavra com brinquedo/objeto, contemplando as Etapas 1 e 2. Os juízes crianças analisaram, em situação de coleta de dados, a familiaridade e a representatividade das palavras-estímulos, apresentado na Etapa 3. Após, realizou-se o estudo piloto- Etapa 4, com as palavras-estímulo selecionadas. Na análise estatística dos juízes especialistas e não especialistas utilizou-se o índice de Concordância do Kappa Fleiss e Gwet. Na análise das respostas dos juízes crianças e no estudo piloto, a análise foi feita em relação ao tipo de resposta da criança, especificamente pontuando a nomeação espontânea do brinquedo/objeto, pontuando de forma qualitativa. RESULTADOS: O resultado da Etapa 1 e 2, e a concordância dos testes estatísticos para os critérios de Familiaridade e Representatividade foi de 45,7% para JE e 76,4% para JNE, e resultado de 100% para a concordância da Representatividade. Foram analisadas 122 palavras, resultantes dos critérios previamente estabelecidos, totalizando 34 palavras (exclusão de uma palavra por parte das pesquisadoras), totalizando 33 palavras-estímulos. Na Etapa 3, das 33 palavras-estímulos aplicadas, nove apresentaram pontuação de nomeação espontânea abaixo do esperado, sendo retestadas para a Etapa 4, Estudo Piloto. O resultado do Estudo Piloto evidenciou que das nove palavras-estímulos retestadas, quatro delas ainda apresentaram pontuação abaixo, sendo excluídas do estudo. Portanto com a aplicação do estudo piloto a lista expandida do PEEPS-BP, resultou em 29 palavras. CONCLUSÃO: O PEEPS-BP - Lista Expandida apresentou evidências de validade de conteúdo satisfatórias para a adaptação transcultural do teste.
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Idioma , Linguística , Criança , Humanos , Pré-Escolar , Brasil , Projetos Piloto , Psicometria , Traduções , Comparação Transcultural , Reprodutibilidade dos Testes , Inquéritos e QuestionáriosRESUMO
This work reports the construction of an HIV-specific genosensor through the modification of carbon screen-printed electrodes (CSPE) with graphene quantum dots decorated with L-cysteine and gold nanoparticles (cys-GQDs/AuNps). Cys-GQDs were characterized by FT-IR and UV-vis spectra and electronic properties of the modified electrodes were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The modification of the electrode surface with cys-GQDs and AuNps increased the electrochemical performance of the electrode, improving the electron transfer of the anionic redox probe [Fe(CN)6]3-/4- on the electrochemical platform. When compared to the bare surface, the modified electrode showed a 1.7 times increase in effective electrode area and a 29 times decrease in charge transfer resistance. The genosensor response was performed by differential pulse voltammetry, monitoring the current response of the anionic redox probe, confirmed with real genomic RNA samples, making it possible to detect 1 fg/mL. In addition, the genosensor maintained its response for 60 days at room temperature. This new genosensor platform for early detection of HIV, based on the modification of the electrode surface with cys-GQDs and AuNps, discriminates between HIV-negative and positive samples, showing a low detection limit, as well as good specificity and stability, which are relevant properties for commercial application of biosensors.
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Técnicas Biossensoriais , Grafite , Infecções por HIV , Nanopartículas Metálicas , Pontos Quânticos , Humanos , Grafite/química , Pontos Quânticos/química , Ouro/química , Espectroscopia de Infravermelho com Transformada de Fourier , Nanopartículas Metálicas/química , Técnicas Eletroquímicas/métodos , Cisteína , Técnicas Biossensoriais/métodos , Eletrodos , RNA , Limite de DetecçãoRESUMO
Breast milk is known to contain bioactive peptides that are released during digestion, being a major source of bioactive peptides to the new-born, some of which act against invading pathogens. However, the formation of bioactive peptides during digestion of human colostrum remains largely uninvestigated. This study aimed to investigate the formation of peptides during simulated digestion of human colostrum from adult women and to prospect antimicrobial peptides. For this purpose, we used high-resolution MS to monitor the release of peptides during in vitro digestion. Bioinformatics was used for the prospection of antimicrobial activity of peptides. During simulated digestion (oral, gastric and duodenal phases), 2318 peptide sequences derived from 112 precursor proteins were identified. At the end of simulated digestion, casein-derived peptide sequences were the most frequently observed. Among precursors, some proteins were seen for the first time in this study. The resulting peptides were rich in proline, glutamine, valine and leucine residues, providing characteristic traits of antimicrobial peptides. From bioinformatics analysis, seven peptides showed potentially high antimicrobial activity towards bacteria, viruses and fungi, from which the latter was the most prominent predicted activity. Antimicrobial peptides released during digestion may provide a defence platform with controlled release for the new-born.
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Anti-Infecciosos , Colostro , Adulto , Gravidez , Humanos , Feminino , Proteólise , Colostro/química , Espectrometria de Massas em Tandem , Peptídeos/química , Leite Humano/metabolismo , Cromatografia Líquida , Caseínas/metabolismo , Peptídeos Antimicrobianos , Proteômica/métodos , Anti-Infecciosos/farmacologia , Anti-Infecciosos/análise , Anti-Infecciosos/metabolismo , DigestãoRESUMO
RESUMO Objetivo Realizar a adaptação transcultural do Instrumento Profiles of Early Expressive Phonological Skills- Brazilian Portuguese (PEEPS-BP) - Lista expandida, realizando a validação de conteúdo. Método Estudo de adaptação transcultural, quantitativo e transversal, considerando critérios psicométricos. Realizou-se o estudo da lista de 423 palavras do Inventário de Desenvolvimento Comunicativo- MacArthur- Palavras e Sentenças, adaptado para o Português Brasileiro. O método foi dividido em quatro Etapas. A lista foi julgada por juízes especialistas (JE) e juízes não especialistas (JNE), considerando como critério a familiaridade da palavra para uma criança entre 24 e 36 meses, e a representatividade da palavra com brinquedo/objeto, contemplando as Etapas 1 e 2. Os juízes crianças analisaram, em situação de coleta de dados, a familiaridade e a representatividade das palavras-estímulos, apresentado na Etapa 3. Após, realizou-se o estudo piloto- Etapa 4, com as palavras-estímulo selecionadas. Na análise estatística dos juízes especialistas e não especialistas utilizou-se o índice de Concordância do Kappa Fleiss e Gwet. Na análise das respostas dos juízes crianças e no estudo piloto, a análise foi feita em relação ao tipo de resposta da criança, especificamente pontuando a nomeação espontânea do brinquedo/objeto, pontuando de forma qualitativa. Resultados O resultado da Etapa 1 e 2, e a concordância dos testes estatísticos para os critérios de Familiaridade e Representatividade foi de 45,7% para JE e 76,4% para JNE, e resultado de 100% para a concordância da Representatividade. Foram analisadas 122 palavras, resultantes dos critérios previamente estabelecidos, totalizando 34 palavras (exclusão de uma palavra por parte das pesquisadoras), totalizando 33 palavras-estímulos. Na Etapa 3, das 33 palavras-estímulos aplicadas, nove apresentaram pontuação de nomeação espontânea abaixo do esperado, sendo retestadas para a Etapa 4, Estudo Piloto. O resultado do Estudo Piloto evidenciou que das nove palavras-estímulos retestadas, quatro delas ainda apresentaram pontuação abaixo, sendo excluídas do estudo. Portanto com a aplicação do estudo piloto a lista expandida do PEEPS-BP, resultou em 29 palavras. Conclusão O PEEPS-BP - Lista Expandida apresentou evidências de validade de conteúdo satisfatórias para a adaptação transcultural do teste.
ABSTRACT Purpose To carry out the cross-cultural adaptation of the Instrument Profiles of Early Expressive Phonological Skills- Brazilian Portuguese (PEEPS-BP) - Expanded list, performing content validation. Methods Cross-cultural, quantitative and cross- sectional adaptation study, considering psychometric criteria. A study was carried out on the list of 423 words from the Communicative Development Inventory - MacArthur - Words and Sentences, adapted to Brazilian Portuguese. The method was divided into four steps. The list was judged by expert judges (JE) and non-specialist judges (JNE), considering as a criterion the familiarity of the word for a child aged between 24 and 36 months, and the representativeness of the word with toy/object, contemplating Steps 1 and 2. The child judges analyzed, in a data collection situation, the familiarity and representativeness of the stimulus-words, presented in Step 3. Afterwards, the pilot study - Step 4, was carried out with the selected stimulus-words. In the statistical analysis by expert and non-specialist judges, the Fleiss' Kappa and Gwet Concordance index was used. In the analysis of the responses of the child judges and in the Pilot Study, the analysis was made in relation to the type of response of the child, specifically scoring the spontaneous naming of the toy/object, scoring qualitatively. Results The result of Steps 1 and 2, and the agreement of the statistical tests for the Familiarity and Representativeness criteria was 45.7% for JE and 76.4% for JNE, and a result of 100% for the agreement of Representativeness. A total of 122 words were analyzed, resulting from previously established criteria, totaling 34 words (exclusion of one word by the researchers), totaling 33 stimulus-words. In Stage 3, of the 33 stimulus-words applied, nine presented spontaneous naming scores below expectations, being retested for Step 4, the Pilot Study. The result of the Pilot Study showed that of the nine retested stimulus-words, four of them still had a score below, being excluded from the study. Therefore, with the application of the Pilot Study, the expanded list of PEEPS-BP resulted in 29 words. Conclusion The PEEPS-BP - Expanded List showed satisfactory evidence of content validity for the cross-cultural adaptation of the test.
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PURPOSE: To present the sensitivity and specificity and establish cutoff points (receiver operating characteristic [ROC] curve) for the PraxiFala Battery. METHOD: The sample included 308 Brazilian-speaking children aged 3;0-7;11. Twenty-one children had motor speech disorders (MSD), 58 children had phonological disorder (PD), and 229 had typical speech (TS) development. Participants were administered the PraxiFala Battery, which contains verbal (word and sentence production), nonverbal (orofacial praxis), and diadochokinetic tasks. The sensitivity and specificity of items in each task were then calculated using ROC curves. RESULT: Total scores on the verbal (word production), nonverbal (orofacial praxis), and diadochokinetic tasks had good sensitivity and specificity. The only scores with poor sensitivity and specificity in differentiating between TS, PD, and MSD were consistency and prosody in the verbal tasks (sentence production), and item /ta/ in the diadochokinetic task. Area under the ROC curve (AUC) values were greater than 0.7 for most items in the comparison between TS vs. MSD and PD vs. MSD. AUC values were poor or fair among children with TS and PD, suggesting that this instrument may not be accurate in identifying these groups. CONCLUSION: The verbal, nonverbal, and diadochokinetic tasks in the PraxiFala Battery had good sensitivity and specificity.
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PURPOSE: To develop and validate an experimental verbal episodic memory task in Spanish. METHODS: Six encoding blocks were elaborated, three deep and three superficial, each one with different demands of cognitive effort. The blocks were reviewed by four expert judges and tested in a pilot application. The agreement was assessed on whether the task allowed combined processing level and cognitive effort to be manipulated during incidental encoding of words, as well as clarity of instructions, examples, and workflow. RESULTS: Variables such as lexical availability, metrics, and strength of association were useful to differentiate the cognitive effort between each block. The judges agreed that the processing blocks allowed a combined manipulation of the level of processing and cognitive effort and that the instructions are precise. After the pilot, the participants agreed that the instructions, examples, and way of working were easy to understand and perform. CONCLUSION: The results provide evidence of validity related to the content for the proposed experimental task, thus becoming a viable alternative to consider in research aimed at identifying environmental factors that contribute to compensating the defects shown by episodic memory with age.
PROPÓSITO: Elaborar y validar una tarea experimental de memoria episódica verbal en español. MÉTODO: Se elaboraron seis bloques de codificación: tres profundos y tres superficiales, cado uno con distintas demandas de esfuerzo cognitivo. Los bloques fueron revisados por cuatro jueces expertos y examinados en una aplicación piloto. Se evaluó la concordancia respecto a si la tarea permitía manipular combinadamente el nivel de procesamiento y el esfuerzo cognitivo durante la codificación incidental de palabras, así como la claridad de las instrucciones, ejemplos y dinámica de trabajo. RESULTADOS: Variables como la disponibilidad léxica, metría y fuerza de asociación fueron útiles para diferenciar el esfuerzo cognitivo entre cada bloque. Los jueces concordaron que los bloques de procesamiento admiten una manipulación combinada del nivel de procesamiento y esfuerzo cognitivo y que las instrucciones son precisas. Luego del pilotaje, los participantes concordaron que las instrucciones, ejemplos y forma de trabajo eran fácil de comprender y realizar. CONCLUSIÓN: Los resultados proporcionan evidencia de validez relacionada con el contenido para la tarea experimental propuesta, transformándose con ello en una alternativa viable de considerar en investigaciones orientadas a identificar factores ambientales que contribuyan a compensar los defectos que muestra la memoria episódica con la edad.
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Memória Episódica , Humanos , IdiomaRESUMO
Among the malignant tumors, breast cancer is the most commonly diagnosed worldwide, being the most prevalent in women. Photobiomodulation has been used for wound healing, swelling and pain reduction, and muscle repair. The application of photobiomodulation in cancer patients has been controversial. Therefore, a better understanding of radiation-induced effects involved in photobiomodulation on cancer cells is needed. Thus, this study aimed to investigate the effects of exposure to low-power lasers and LEDs on cell viability, migration, and invasion in human breast cancer cells. MCF-7 and MDA-MB-231 cells were irradiated with a low-power red laser (23, 46, and 69 J/cm2, 0.77 W/cm2) and blue LED (160, 321, and 482 J/cm2, 5.35 W/cm2), alone or in combination. Cell viability was assessed using the WST-1 assay, cell migration was evaluated using the wound healing assay, and cell invasion was performed using the Matrigel transwell assay. Viability and migration were not altered in MCF-7 and MDA-MB-231 cultures after exposure to low-power red laser and blue LED. However, there was a decrease in cell invasion from the cultures of the two cell lines evaluated. The results suggest that photobiomodulation induced by low-power red laser and blue LED does not alter cell viability and migration but decreases cell invasion in human breast cancer cells.
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Neoplasias da Mama , Humanos , Feminino , Neoplasias da Mama/radioterapia , Linhagem Celular , Movimento Celular , Sobrevivência Celular , LasersRESUMO
Dengue virus is an important circulating arbovirus in Brazil responsible for high morbidity and mortality worldwide, representing a huge economic and social burden, in addition to affecting public health. In this study, the biological activity, toxicity, and antiviral activity against dengue virus type 2 (DENV-2) of tizoxanide (TIZ) was evaluated in Vero cell culture. TIZ has a broad spectrum of action in inhibiting different pathogens, including bacteria, protozoa, and viruses. Cells were infected for 1 h with DENV-2 and then treated for 24 h with different concentrations of the drug. The quantification of viral production indicated the antiviral activity of TIZ. The protein profiles in infected Vero cells treated and not treated with TIZ were analyzed using the label-free quantitative proteomic approach. TIZ was able to inhibit virus replication mainly intracellularly after DENV-2 penetration and before the complete replication of the viral genome. Additionally, the study of the protein profile of infected not-treated and infected-treated Vero cells showed that TIZ interferes with cellular processes such as intracellular trafficking and vesicle-mediated transport and post-translational modifications when added after infection. Our results also point to the activation of immune response genes that would eventually lead to a decrease of DENV-2 production. TIZ is a promising therapeutic molecule for the treatment of DENV-2 infections.
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Vírus da Dengue , Dengue , Chlorocebus aethiops , Animais , Humanos , Antivirais/farmacologia , Antivirais/uso terapêutico , Células Vero , Dengue/tratamento farmacológico , Vírus da Dengue/genética , Proteômica , Replicação ViralRESUMO
Resumo O presente artigo compara os modelos e as desigualdades territoriais no financiamento de duas políticas sociais pilares do estado de bem-estar social e com alto grau de descentralização territorial na Espanha e no Brasil: educação e saúde. A análise utiliza bibliografia especializada, legislação nacional e documentos governamentais para descrever as políticas e seus mecanismos de financiamento. Dados fiscais são usados para apresentar os gastos e analisar as desigualdades dos governos subnacionais no financiamento da educação e da saúde nos dois países. A conclusão é que a experiência espanhola apresenta elevado nivelamento de gastos em saúde e educação nas comunidades autônomas do regime comum, com patamares menores de desigualdade que o observado nos estados e municípios brasileiros. O resultado espanhol é decorrente de um processo incremental de aperfeiçoamento do federalismo fiscal, que culminou em um modelo marcado pela priorização e solidariedade territorial no financiamento das políticas sociais. Esse modelo é uma referência para a análise e discussão do caso brasileiro, que configurou seu federalismo fiscal com pouca preocupação em conciliar eficiência e equidade na distribuição dos recursos entre os entes governamentais, mas apresentou avanços importantes em reformas no financiamento da educação e da saúde.
Resumen El artículo compara los modelos y las desigualdades territoriales en la financiación de dos políticas sociales que son pilares del estado de bienestar y con un alto grado de descentralización territorial en España y Brasil: la educación y la sanidad. El análisis utiliza literatura especializada, legislación nacional y documentos gubernamentales para describir las políticas y sus mecanismos de financiación. Los datos fiscales se utilizan para analizar las desigualdades de los gobiernos subnacionales en la financiación de la educación y la sanidad en ambos países. La conclusión es que la experiencia española muestra una alta nivelación del gasto en salud y educación en las comunidades autónomas de régimen común, con niveles de desigualdad inferiores a los observados en los estados y municipios brasileños. El resultado español es fruto de un proceso de mejora y perfeccionamiento del federalismo fiscal que culminó en un modelo marcado por la priorización y la solidaridad territorial en la financiación de las políticas sociales. Este modelo es una referencia para el análisis y la discusión del caso brasileño, que ha configurado su federalismo fiscal con poca preocupación por conciliar la eficiencia y la equidad en la distribución de los recursos entre las entidades gubernamentales, pero que ha presentado importantes avances en las reformas de la financiación de la educación y la sanidad.
Abstract The article compares the patterns and territorial inequalities in the funding of two social policies that are pillars of the welfare state and present a high degree of territorial decentralization in Spain and Brazil: education and health. The analysis uses specialist literature, national legislation and government documents to describe the policies and their financing mechanisms. Fiscal data are used to analyze subnational government inequalities in the funding of education and health in both countries. The conclusion is that the Spanish experience has significantly leveled spending on health and education between the autonomous communities of common regime, with lower levels of inequality than those observed in Brazilian states and municipalities. The Spanish result derives from an incremental process of improvement of the country's fiscal federalism, which culminated in a model marked by prioritization and territorial solidarity in the funding of social policies. This model is reference for the analysis and discussion of the Brazilian case, which has configured its fiscal federalism with little concern for reconciling efficiency and equity in the distribution of resources between subnational governments, but which has presented important advances in the reforms of education and health funding.
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Política Pública , Fatores Socioeconômicos , Espanha , Brasil , Saúde , EducaçãoRESUMO
RESUMEN Propósito Elaborar y validar una tarea experimental de memoria episódica verbal en español. Método Se elaboraron seis bloques de codificación: tres profundos y tres superficiales, cado uno con distintas demandas de esfuerzo cognitivo. Los bloques fueron revisados por cuatro jueces expertos y examinados en una aplicación piloto. Se evaluó la concordancia respecto a si la tarea permitía manipular combinadamente el nivel de procesamiento y el esfuerzo cognitivo durante la codificación incidental de palabras, así como la claridad de las instrucciones, ejemplos y dinámica de trabajo. Resultados Variables como la disponibilidad léxica, metría y fuerza de asociación fueron útiles para diferenciar el esfuerzo cognitivo entre cada bloque. Los jueces concordaron que los bloques de procesamiento admiten una manipulación combinada del nivel de procesamiento y esfuerzo cognitivo y que las instrucciones son precisas. Luego del pilotaje, los participantes concordaron que las instrucciones, ejemplos y forma de trabajo eran fácil de comprender y realizar. Conclusión Los resultados proporcionan evidencia de validez relacionada con el contenido para la tarea experimental propuesta, transformándose con ello en una alternativa viable de considerar en investigaciones orientadas a identificar factores ambientales que contribuyan a compensar los defectos que muestra la memoria episódica con la edad.
ABSTRACT Purpose To develop and validate an experimental verbal episodic memory task in Spanish. Methods Six encoding blocks were elaborated, three deep and three superficial, each one with different demands of cognitive effort. The blocks were reviewed by four expert judges and tested in a pilot application. The agreement was assessed on whether the task allowed combined processing level and cognitive effort to be manipulated during incidental encoding of words, as well as clarity of instructions, examples, and workflow. Results Variables such as lexical availability, metrics, and strength of association were useful to differentiate the cognitive effort between each block. The judges agreed that the processing blocks allowed a combined manipulation of the level of processing and cognitive effort and that the instructions are precise. After the pilot, the participants agreed that the instructions, examples, and way of working were easy to understand and perform. Conclusion The results provide evidence of validity related to the content for the proposed experimental task, thus becoming a viable alternative to consider in research aimed at identifying environmental factors that contribute to compensating the defects shown by episodic memory with age.
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ABSTRACT Purpose: to carry out the semantic analysis of a list of words that will compose a virtual tool for speech assessment for children and adolescents. Methods: twenty-three participants, aged between 2 years old and 17 years and 11 months old, from the central region of Rio Grande do Sul, Brazil, assigned the concept of 91 words. Data analysis was performed quantitatively, considering the concept of each word as correct or incorrect. Content Validity Ratio (RVC) and Gwet's first-order agreement coefficient (AC1) statistical calculation were calculated. Results: from the word list analyzed, 42 stimuli presented CVR = 1; 30 words obtained CVR = 0.9; 11 with CVR = 0.8; six with CVR = 0.7; two had CVR = 0.4. Gwet's AC1 statistical calculation resulted in AC1 = 0.92 [CI = 0.90 - 0.94] for semantic analysis. Conclusion: the list consisted of 91 semantically validated words that can be used to assess the speech production of children and adolescents.
RESUMO Objetivo: realizar a análise semântica da lista de palavras que comporá um Instrumento Virtual de Avaliação da Fala para crianças e adolescentes. Métodos: vinte e três participantes, com idades entre 2 e 17 anos e 11 meses, atribuíram o conceito de 91 palavras. A análise dos dados foi realizada de forma quantitativa, considerando a conceituação de cada palavra como correta ou incorreta. Foi realizado cálculo da Razão de Validade de Conteúdo (RVC) e o cálculo estatístico first-order agreement coefficient (AC1) de Gwet. Resultados: da lista de palavras analisada, 42 estímulos apresentaram RVC=1; 30 palavras obtiveram RVC=0,9; 11 com RVC=0,8; seis com RVC=0,7; duas apresentaram RVC=0,4. O cálculo estatístico AC1 de Gwet resultou em AC1=0,92 [IC=0,90 - 0,94] para a análise semântica. Conclusão: a lista ficou constituída de 91 palavras validadas semanticamente e que podem servir para avaliação da produção de fala de crianças e adolescentes.
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Hepatitis C virus (HCV) is responsible for more than 180 million infections worldwide, and about 80â% of infections are reported in Low and Middle-income countries (LMICs). Therapy is based on the administration of interferon (INF), ribavirin (RBV) or more recently Direct-Acting Antivirals (DAAs). However, amino acid substitutions associated with resistance (RAS) have been extensively described and can contribute to treatment failure, and diagnosis of RAS requires considerable infrastructure, not always locally available. Dried serum spots (DSS) sampling is an alternative specimen collection method, which embeds drops of serum onto filter paper to be transported by posting to a centralized laboratory. Here, we assessed feasibility of genotypic analysis of HCV from DSS in a cohort of 80 patients from São Paulo state Brazil. HCV RNA was detected on DSS specimens in 83â% of samples of HCV infected patients. HCV genotypes 1a, 1b, 2a, 2c and 3a were determined using the sequence of the palm domain of NS5B region, and RAS C316N/Y, Q309R and V321I were identified in HCV 1b samples. Concerning therapy outcome, 75â% of the patients who used INF +RBV as a previous protocol of treatment did not respond to DAAs, and 25â% were end-of-treatment responders. It suggests that therapy with INF plus RBV may contribute for non-response to a second therapeutic protocol with DAAs. One patient that presented RAS (V321I) was classified as non-responder, and combination of RAS C316N and Q309R does not necessarily imply in resistance to treatment in this cohort of patients. Data presented herein highlights the relevance of studying circulating variants for a better understanding of HCV variability and resistance to the therapy. Furthermore, the feasibility of carrying out genotyping and RAS phenotyping analysis by using DSS card for the potential of informing future treatment interventions could be relevant to overcome the limitations of processing samples in several location worldwide, especially in LMICs.
RESUMO
In this work, we report the construction of a novel electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4-aminophenol) and sensitized with a specific DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was efficient in detecting genomic DNA with a linear range from 1.176 to 4.825 µg mL-1 and detection limit of 35.69 ng mL-1 (4.63 IU ml-1 or 25.93 copies.ml-1), which is better than the 10.00 IU ml-1 limit of reference method, real-time PCR, used in point of care. EIS analysis shows that the genosensor resistance increased exponentially with the concentration of the genomic DNA target. This novel platform has advantages to its applicability in real samples, such as good sensitivity, selectivity, low sample volume, and fast assay time (36 min), thus interesting for application in the diagnosis of hepatitis B virus in blood plasma. Also, the ease of synthesis of the low-cost polymer by electrosynthesis directly on the electrode surface allows the translation of the platform to portable devices.
Assuntos
Técnicas Biossensoriais , Grafite , Hepatite B , Técnicas Biossensoriais/métodos , DNA/química , Técnicas Eletroquímicas/métodos , Eletrodos , Grafite/química , Hepatite B/diagnóstico , Vírus da Hepatite B/genética , Humanos , Limite de Detecção , PlasmaRESUMO
Difficult in literacy skills are often seen in children with Childhood Apraxia of Speech (CAS). This occurs because oral language has direct relationship with reading and writing learning. The purpose of this study was to evaluate the performance in the accuracy task of an integrated phonoarticulatory awareness, motor skills and literacy intervention of three children with CAS. Three boys between 5;3 and 5;8 years of age, with CAS, were offered 2 hours per week of therapy sessions based on literacy and motor skills. The children were assessed before and after therapy and at a maintenance assessment 1 month after the treatment ceased. The children improved on the accuracy task considering their deficits level. Improvement was maintained at the maintenance assessment. Therapy based on literacy considering phonoarticulatory awareness and motor skills can help children with CAS, but the severity of the children's communication problems must be taken into consideration.
Assuntos
Apraxias , Alfabetização , Apraxias/terapia , Criança , Humanos , Masculino , Destreza Motora , Leitura , Fala , Distúrbios da Fala/terapiaRESUMO
PURPOSE: To present and analyze the acquisition segmental curve of Brazilian Portuguese in simple and complex onset position and coda position. METHODS: 857 children with typical phonological acquisition participated in it, aged between 3:0 and 8:11, divided into age groups every 6 months. Participants were assessed using INFONO phonological assessment software. After analyzing the results, acquisition curves were drawn up for the segments analyzed in the different structures (simple and complex onset position and coda position). RESULTS: It was noted that, in simple onset position, some segments were acquired before 3:0 (stops, nasal and fricative, /f, v, s, z/. The /Ê/ and /l/ segments were acquired at 3:0, /Ê/ and /x/ at 3:6, /Ê/ at 4:0, /r/ at 4:6; in coda position /N/ and /L/ were acquired before of 3:0, /S/ at 3:6 and /r/ at 4:6; in complex onset position, the structures composed by Fricative + /r/ and Stop + /r/ were acquired at 5:0, Stop + /l/ at 5:6 and Fricative + /l/ at 6:0. CONCLUSION: Analyzing the acquisition curve is essential, as it provides a reference on the age of acquisition of segments in different syllabic structures. The acquisition curve contributes to the early identification of delays in phonological acquisition process enabling a timely referral for speech therapy intervention.
OBJETIVO: Apresentar e analisar a curva de aquisição segmental do Português Brasileiro em onset simples, complexo e coda. MÉTODO: Participaram 857 crianças com aquisição fonológica típica, com idades entre 3:0 e 8:11, divididos em faixas etárias de 6 em 6 meses. Os participantes foram avaliados, por meio do software INFONO de avaliação fonológica. Após a análise dos resultados foram elaboradas curvas de aquisição dos segmentos analisados nas diferentes estruturas (onset simples, complexo e coda). RESULTADOS: Observou-se que, em onset simples, alguns segmentos estavam adquiridos antes dos 3:0 (plosivas, nasais e fricativas /f, v, s, z/). Os segmentos /Ê/ e /l/ foram adquiridos aos 3:0, /Ê/ e /x/ aos 3:6, /Ê/ aos 4:0, /ɾ/ aos 4:6; em coda, /N/ e /L/ foram adquiridos antes dos 3:0, /S/ aos 3:6 e /ɾ/ aos 4:6; em onset complexo, as estruturas compostas por Fricativa+/ɾ/ e Plosiva+/ɾ/ foram adquiridas aos 5:0, Plosiva+/l/ aos 5:6 e Fricativa + /l/ aos 6:0. CONCLUSÃO: Analisar a curva de aquisição é fundamental, pois fornece referência sobre a idade de aquisição dos segmentos nas diferentes estruturas silábicas. A curva de aquisição contribui para a identificação precoce de atrasos no processo de aquisição fonológica possibilitando um encaminhamento oportuno para a intervenção fonoaudiológica.
Assuntos
Idioma , Fonética , Brasil , Criança , Humanos , Lactente , Fala , Medida da Produção da FalaRESUMO
BACKGROUND: AIMS: To describe typical phonological development of Brazilian Portuguese (BP)-speaking children, considering the following parameters: age of customary production, acquisition and mastery. METHODS & PROCEDURES: Data were collected from 857 children aged between 3 years and 8 years 11 months with typical language and speech development. The sample was grouped into 6-month age bands. The data were analysed using one-way analysis of variance (ANOVA) followed by Games-Howell post-hoc tests. OUTCOMES & RESULTS: Stops (/p, b, t, d, k, g/), nasals (/m, n, ɲ/) and some fricatives (/f, v, s, z/) were mastered before the age of 3 years (age of mastery). The age of acquisition for phonemes /Ê, Ê/ was 3;6, though both were only mastered at 4;0 years. The liquid /l/ was acquired at 3;0 and mastered at age 3;6, while /x/ was acquired and mastered at age 3;6. The phoneme /Ê/ was acquired at 7;0 and mastered at age 8;6. The tap /ɾ/ was acquired between the ages of 4;0 and 4;6, and mastered at 4;6. In coda position, /n, l/ were acquired at 3;0, while /s/ was mastered at 4;6 and /ɾ/ between 4;6 and 5;0 years. Clusters involving /ɾ/ were acquired at 6;0, while those with /l/ were acquired between the ages of 6;6 and 7;0. CONCLUSIONS & IMPLICATIONS: This study presented acquisition for consonants in BP and can be used as a reference for the assessment of developmental speech disorders. In this study, stops and nasals were acquired first, followed by fricatives and, lastly, liquids. This finding is corroborated by previous studies in BP and other languages. WHAT THIS PAPER ADDS: What is already known on the subject What this paper adds to existing knowledge What are the potential or actual clinical implications of this work?
Assuntos
Desenvolvimento da Linguagem , Idioma , Fonética , Brasil , Criança , Linguagem Infantil , Pré-Escolar , Humanos , Medida da Produção da FalaRESUMO
ABSTRACT Difficult in literacy skills are often seen in children with Childhood Apraxia of Speech (CAS). This occurs because oral language has direct relationship with reading and writing learning. The purpose of this study was to evaluate the performance in the accuracy task of an integrated phonoarticulatory awareness, motor skills and literacy intervention of three children with CAS. Three boys between 5;3 and 5;8 years of age, with CAS, were offered 2 hours per week of therapy sessions based on literacy and motor skills. The children were assessed before and after therapy and at a maintenance assessment 1 month after the treatment ceased. The children improved on the accuracy task considering their deficits level. Improvement was maintained at the maintenance assessment. Therapy based on literacy considering phonoarticulatory awareness and motor skills can help children with CAS, but the severity of the children's communication problems must be taken into consideration.
RESUMO Dificuldade nas habilidades de alfabetização são frequentemente observadas em crianças com Apraxia da Fala na Infância (AFI). Isso ocorre porque a linguagem oral tem relação direta com a aprendizagem da leitura e da escrita. O objetivo deste estudo foi avaliar o desempenho na tarefa de acurácia de uma intervenção integrada de consciência fonoarticulatória, habilidades motoras e letramento de três crianças com AFI. Três meninos com idade entre 5:3 e 5:8 anos, com AFI, receberam 2 horas semanais de sessões de terapia baseadas na alfabetização e habilidades motoras. As crianças foram avaliadas antes e após a terapia e em uma avaliação de manutenção 1 mês após o término do tratamento. As crianças melhoraram na tarefa de precisão, considerando o nível de seus déficits. A melhora foi mantida na avaliação de manutenção. A terapia baseada na alfabetização, considerando a consciência fonológica e as habilidades motoras, pode ajudar as crianças com AFI, mas a gravidade dos problemas de comunicação das crianças deve ser levada em consideração.
RESUMO
RESUMO Objetivo Apresentar e analisar a curva de aquisição segmental do Português Brasileiro em onset simples, complexo e coda. Método Participaram 857 crianças com aquisição fonológica típica, com idades entre 3:0 e 8:11, divididos em faixas etárias de 6 em 6 meses. Os participantes foram avaliados, por meio do software INFONO de avaliação fonológica. Após a análise dos resultados foram elaboradas curvas de aquisição dos segmentos analisados nas diferentes estruturas (onset simples, complexo e coda). Resultados Observou-se que, em onset simples, alguns segmentos estavam adquiridos antes dos 3:0 (plosivas, nasais e fricativas /f, v, s, z/). Os segmentos /ʃ/ e /l/ foram adquiridos aos 3:0, /ʒ/ e /x/ aos 3:6, /ʎ/ aos 4:0, /ɾ/ aos 4:6; em coda, /N/ e /L/ foram adquiridos antes dos 3:0, /S/ aos 3:6 e /ɾ/ aos 4:6; em onset complexo, as estruturas compostas por Fricativa+/ɾ/ e Plosiva+/ɾ/ foram adquiridas aos 5:0, Plosiva+/l/ aos 5:6 e Fricativa + /l/ aos 6:0. Conclusão Analisar a curva de aquisição é fundamental, pois fornece referência sobre a idade de aquisição dos segmentos nas diferentes estruturas silábicas. A curva de aquisição contribui para a identificação precoce de atrasos no processo de aquisição fonológica possibilitando um encaminhamento oportuno para a intervenção fonoaudiológica.
ABSTRACT Purpose To present and analyze the acquisition segmental curve of Brazilian Portuguese in simple and complex onset position and coda position. Methods 857 children with typical phonological acquisition participated in it, aged between 3:0 and 8:11, divided into age groups every 6 months. Participants were assessed using INFONO phonological assessment software. After analyzing the results, acquisition curves were drawn up for the segments analyzed in the different structures (simple and complex onset position and coda position). Results It was noted that, in simple onset position, some segments were acquired before 3:0 (stops, nasal and fricative, /f, v, s, z/. The /ʃ/ and /l/ segments were acquired at 3:0, /ʒ/ and /x/ at 3:6, /ʎ/ at 4:0, /r/ at 4:6; in coda position /N/ and /L/ were acquired before of 3:0, /S/ at 3:6 and /r/ at 4:6; in complex onset position, the structures composed by Fricative + /r/ and Stop + /r/ were acquired at 5:0, Stop + /l/ at 5:6 and Fricative + /l/ at 6:0. Conclusion Analyzing the acquisition curve is essential, as it provides a reference on the age of acquisition of segments in different syllabic structures. The acquisition curve contributes to the early identification of delays in phonological acquisition process enabling a timely referral for speech therapy intervention.