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Advancement in SARS-CoV-2 diagnosis: A new and stable electrochemical biosensor for genomic RNA detection.
Gomide, José A L; Moço, Anna C R; Tanaka, Leonardo T R; Alexandre, Maikon R A; Roca, Tárcio Peixoto; Vieira Dall'acqua, Deusilene Souza; Costa Nunes Soares, Márcia M; Oliveira, Ronaldo J; Lima, Renata C; Madurro, João M; Brito-Madurro, Ana G.
Afiliação
  • Gomide JAL; Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil.
  • Moço ACR; Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil.
  • Tanaka LTR; Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil.
  • Alexandre MRA; Institute of Chemistry, Federal University if Uberlandia, Uberlândia, Brazil.
  • Roca TP; Oswaldo Cruz Foundation, Rondonia Unity, Porto Velho, Brazil.
  • Vieira Dall'acqua DS; Oswaldo Cruz Foundation, Rondonia Unity, Porto Velho, Brazil.
  • Costa Nunes Soares MM; Adolfo Lutz Institute, Regional Laboratory, São José do Rio Preto, Brazil.
  • Oliveira RJ; Department of Physics, Institute of Exact, Natural and Educational Sciences, Federal University of Triângulo Mineiro, Uberaba, Brazil.
  • Lima RC; Institute of Chemistry, Federal University if Uberlandia, Uberlândia, Brazil.
  • Madurro JM; Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil; Institute of Chemistry, Federal University if Uberlandia, Uberlândia, Brazil.
  • Brito-Madurro AG; Institute of Biotechnology, Federal University of Uberlandia, Uberlândia, Brazil. Electronic address: agbrito@ufu.br.
Bioelectrochemistry ; 161: 108798, 2024 Aug 17.
Article em En | MEDLINE | ID: mdl-39243734
ABSTRACT
Coronavirus disease (COVID-19) is caused by infection with the SARS-CoV-2 virus, having already caused more than seven million deaths worldwide. Conventional techniques for SARS-CoV-2 detection have limitations, as high cost, low specificity, and longer analysis time, among others. Biosensors emerge as a necessary alternative to overcome the difficulties of current diagnostics. This paper reports a sensor platform where silver-doped zinc oxide nanomaterial (AgZnONp) was used onto carbon screen-printed electrode and ethidium bromide as indicator for development of a specific electrochemical genosensor for COVID-19. This genosensor demonstrated good linearity between the concentrations of 5.62 × 104-5.62 copies/mL and a detection limit of 5 copies/mL with gRNA in patient's samples, with a response time within 30 min. Molecular modeling and morphological analysis are in agreement with obtained electrochemical results. Additional techniques such as AFM, SEM, and EIS were conducted to characterize the morphological and electrochemical properties of the biosensor's surface. The biosensor was also capable of detecting the target presence in spiked samples and demonstrated a stability of 60 days, higher than other similar biosensors for SARS-CoV-2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioelectrochemistry Assunto da revista: BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioelectrochemistry Assunto da revista: BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda