RESUMO

Dihydrofolate reductase (DHFR) is an essential enzyme for nucleotide metabolism used to obtain energy and structural nucleic acids. Schistosoma mansoni has all the pathways for pyrimidine biosynthesis, which include the thymidylate cycle and, consequentially, the DHFR enzyme. Here, we describe the characterization of Schistosoma mansoni DHFR (SmDHFR) using isothermal titration calorimetry for the enzymatic activity and thermodynamic determination, also the folate analogs inhibition. Moreover, X-ray crystallography was used to determine the enzyme atomic model at 1.95 Å.

Assuntos

Schistosoma mansoni/enzimologia , Tetra-Hidrofolato Desidrogenase/metabolismo , Animais , Calorimetria , Cristalografia por Raios X , Ensaios Enzimáticos , Ácido Fólico/análogos & derivados , Congelamento , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Síncrotrons , Tetra-Hidrofolato Desidrogenase/química , Tetra-Hidrofolato Desidrogenase/genética , Tetra-Hidrofolato Desidrogenase/isolamento & purificação