RESUMO
OBJECTIVE: The aim of this study was to compare the endometrial expression of milk fat globule-EGF factor 8 (MFG-E8), its receptor integrin αvß3, and leukemia inhibitory factor (LIF) in patients with endometriosis and infertility and in healthy fertile patients during the window of implantation. METHODS: Five patients with peritoneal endometriosis and infertility (case group) and four healthy fertile patients (control group) were recruited. All patients were either diagnosed with or ruled out for endometriosis by laparoscopic surgery; the case group underwent surgery for infertility investigation and the control group for tubal ligation. Endometrial biopsies were performed in all patients during the window of implantation (LH+8 to LH+10), and then the samples were analyzed by immunochemistry for MFG-E8, integrin αvß3, and LIF. RESULTS: In patients with endometriosis and infertility, expression of MFG-E8 was significantly increased in the glandular epithelium when compared to healthy fertile patients (p<0.001). Moreover, LIF expression was lower in patients with endometriosis and infertility (p<0.05). Nevertheless, we found no difference in integrin αvß3 expression between the groups (p=0.084). CONCLUSION: This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. Moreover, LIF is also diminished in the endometrium of these patients as shown before.
Assuntos
Antígenos de Superfície/metabolismo , Endometriose/metabolismo , Endométrio/metabolismo , Infertilidade Feminina/metabolismo , Fator Inibidor de Leucemia/metabolismo , Proteínas do Leite/metabolismo , Doenças Peritoneais/metabolismo , Adulto , Estudos de Casos e Controles , Endometriose/patologia , Endométrio/patologia , Feminino , Fertilidade/fisiologia , Humanos , Infertilidade Feminina/patologia , Integrina alfaVbeta3/metabolismo , Doenças Peritoneais/patologia , Estudos ProspectivosRESUMO
Amenorréia é a ausência ou parada anormal de sangramento menstrual na menacme; para conduzir sua investigação, é importante descartar causas fisiológicas (gravidez e puerpério). além disso, deve-se conhecer as diversas situações patológicas que se manifestam como amenorréia, as quais podem ocorrer por disfunção do eixo hipotálamo-hipófise-gonadal o a anatomia do trato genital. nesse artigo serão revisadas as principais etiologias da amenorréia e sua investigação.
Assuntos
Humanos , Feminino , Amenorreia , Amenorreia/etiologiaRESUMO
Os autores fazem uma revisão bibliográfica sobre endometriose associada à infertilidade com enfoque aos aspectos terapêuticos da doença.