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Anemia is the most common hematological disorder affecting humans. In Peru, anemia is a pressing issue that present the most significant concern due to its adverse effects, such as delayed growth and psychomotor development, in addition to a deficiency in cognitive development. Anemia is a significant public health issue in Peru, which has one of the highest prevalence rates in infants in the Latin American and Caribbean (LAC) region, affecting approximately 43.6 % of children under three years nationally as of 2017, with rural areas experiencing a higher prevalence of approximately 53.3 %. In 2019, the prevalence was highest in the Sierra (48.8 %) and Selva regions (44.6 %), whereas the coast had a lower rate of 33.9 % in children under 36 months. Although the composition of the gut microbiota is relatively well described in children, there is little information on the identification of the microbiota in iron-deficiency anemia. There is evidence that diseases or health conditions can change the microbiota, or vice versa. This study aimed to identify the microbiota in children with anemia who did not recover after iron treatment. In a previous study, we found that the phylum Actinobacteria was predominant in the microbiota of children with anemia. These data will be useful for understanding the functionality of the most important bacteria found in each group at the genus or species level, especially the metabolic pathways in which they participate and their links with iron metabolism. Microbial composition data were obtained through next-generation 16S rRNA sequencing (NGS) of stool samples from children with anemia in southern Peru. Numerous studies have underscored the importance of early symbiotic development in infant health and its long-term impact on health. From infancy, modulation of the gut microbiota can promote long-term health. According to the National Institute of Health (NIH), iron-deficiency anemia may cause serious complications, such as fatigue, headaches, restless legs syndrome, heart problems, pregnancy complications, and developmental delays in children. The development of the gut microbiota is regulated by a complex interplay between host and environmental factors. The bidirectional link between the gut microbiota and anemia plays an important role in tracking the gut microbiota and will be useful in understanding the composition of the intestinal microbiota and its implications in anemia, which has now become a public health problem. Our previous study investigated the microbial composition in children with iron-deficiency anemia and revealed the presence of several bacterial groups, including Proteobacteria, Actinobacteria, Firmicutes, and Chloroflexi. In addition, these data may be useful for investigating the association between the intestinal microbiota of children with persistent anemia and those who have recovered.
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Pyrethroid resistance in Aedes aegypti has become widespread after almost two decades of frequent applications to reduce the transmission of mosquito-borne diseases. Because few insecticide classes are available for public health use, insecticide resistance management (IRM) is proposed as a strategy to retain their use. A key hypothesis of IRM assumes that negative fitness is associated with resistance, and when insecticides are removed from use, susceptibility is restored. In Tapachula, Mexico, pyrethroids (PYRs) were used exclusively by dengue control programs for 15 years, thereby contributing to selection for high PYR resistance in mosquitoes and failure in dengue control. In 2013, PYRs were replaced by organophosphates-insecticides from a class with a different mode of action. To test the hypothesis that PYR resistance is reversed in the absence of PYRs, we monitored Ae. aegypti's PYR resistance from 2016 to 2021 in Tapachula. We observed significant declining rates in the lethal concentration 50 (LC50), for permethrin and deltamethrin. For each month following the discontinuation of PYR use by vector control programs, we observed increases in the odds of mosquitoes dying by 1.5% and 8.4% for permethrin and deltamethrin, respectively. Also, knockdown-resistance mutations (kdr) in the voltage-gated sodium channel explained the variation in the permethrin LC50s, whereas variation in the deltamethrin LC50s was only explained by time. This trend was rapidly offset by application of a mixture of neonicotinoid and PYRs by vector control programs. Our results suggest that IRM strategies can be used to reverse PYR resistance in Ae. aegypti; however, long-term commitment by operational and community programs will be required for success.
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Aedes , Dengue , Inseticidas , Nitrilas , Piretrinas , Animais , Humanos , Inseticidas/farmacologia , Permetrina , Aedes/genética , México , Estudos Longitudinais , Mosquitos Vetores/genética , Mutação , Piretrinas/farmacologia , Resistência a Inseticidas/genética , Dengue/prevenção & controleRESUMO
OBJECTIVE: To evaluate indoor use of commercial aerosols for dengue vector mosquito control, and estimate the number of treatable houses per can. MATERIALS AND METHODS: Four aerosol products containing combinations of pyrethroids (two containing propoxur and one containing synergists too), were evaluated with mosquitoes in a room of a Tapachulastyle house. Eight cages containing 20 insecticide susceptible or resistant females were hung from tripods, another set was placed in sheltered areas of the room. From the entrance of the room, one of 4-9 concentrations was sprayed for each aerosol, leaving the mosquitoes for 30 min after sprayed. Mortality was recorded after 24 h and lethal concentrations were calculated. RESULTS: Aerosol A had the highest LC50, with 0.308 g for mosquitoes hanging from tripods and 0.453 g for sheltered mosquitoes; followed by aerosols C, D and B, with statistical differences between types of exposure. CONCLUSIONS: Aerosols B-D could spray 20-25 3-room houses (56 m3-room), killing all resistant mosquitoes. Aerosols may become a good tool for indoor mosquito control, if the optimal concentration and correct spray method are used.
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Aedes , Inseticidas , Piretrinas , Humanos , Animais , Feminino , Inseticidas/farmacologia , Mosquitos Vetores , Controle de Mosquitos/métodos , AerossóisRESUMO
Tapachula, Mexico, a tropical city, is an endemic area for dengue, in addition to several outbreaks in the last decade with chikungunya and zika. As part of the migratory corridor from Central to North America and the risks of scattered infectious diseases that this implies, the identification and distribution of potential disease vectors in and around residential areas are essential in terms of entomological surveillance for the prevention of disease outbreaks. The identification of mosquito species of medical importance coexisting in houses and cemeteries in Tapachula and two semiurban sites in southern Chiapas was investigated. Adult mosquitoes were collected from May to December 2018, resting inside and outside houses and in the tombstones and fallen tree leaves in cemeteries. A total of 10,883 mosquitoes belonging to three vector species were collected across 20 sites; 6738 were from neighborhood houses, of which 55.4% were Culex quinquefasciatus, 41.6% Aedes aegypti, and 2.9% Ae. albopictus. Aedes aegypti was the most common mosquito resting inside houses (56.7%), while Ae. albopictus and Cx. quinquefasciatus were mostly found resting outside houses (75.7%). In the cemeteries, Cx. quinquefasciatus (60.8%) and Ae. albopictus (37.3%) were the most abundant, while Ae. aegypti (1.9%) was the least abundant. This is the first report to identify adults of three major disease vector species coexisting in the domestic environment of urban and semiurban sites and Ae. albopictus adult resting inside of urban houses in Mexico. It would be opportune to consider comprehensive strategies that can be applied in this region to control the three species at the same time and avoid outbreaks of the diseases they transmit.
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Background: Carbapenem-resistant Klebsiella pneumoniae, particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios. Methodology and Results: We report the isolation and characterization of three new phages against Klebsiella pneumoniae ST258 strains recovered from Machángara river wastewater. These new members of the Ackermannviridae family showed stability over a wide temperature and pH range and burst sizes ranging from 6 to 44 plaque-forming units per bacteria. Their genomes were about 157 kilobases, with an average guanine-cytosine content of 46.4% and showed presence of several transfer RNAs, which also allowed us to predict in silico a lytic replicative cycle due to the presence of endolysins and lysozymes. Conclusion: Three lytic phages of Ackermannviridae family were recovered against Klebsiella pneumoniae ST258 strains from sewage; however, further characterization is needed for future consideration as therapeutic alternatives.
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OBJECTIVE: To identify the enzyme-mediated insecticide resistance in Aedes aegypti in Tapachula, Mexico. MATERIALS AND METHODS: Biochemical assays were undertaken to determine the enzyme levels in mosquitoes from 22 sites collected in 2018 and 2020 in Tapachula. Results of 2018 were correlated with the resistance to insecticides pub-lished. RESULTS: Mosquitoes had higher levels than those of the susceptible strain in 2018 and 2020 respectively of α-esterases in 15 and 12 sites; ß-esterases in 7 and 6 sites; glutathione-S-transferases in 11 and 19 sites; ρNPA-esterases in 21 and 17 sites; and cytochromes P450 in 20 and 22 sites. In mosquitoes of 2018, there was a moderate correlation between previously documented Malathion resistance ratios and the insensitive acetylcholinesterase (r=0.459, p= 0.03). CONCLUSIONS: The elevated enzyme levels found indicate its contribution to the resistance to pyrethroids and organo-phosphates already published in mosquitoes from Tapachula. Bioassays using enzyme inhibitors resulted in greater mor-tality, confirming that metabolism contributes to resistance.
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Aedes , Dengue , Animais , Humanos , Acetilcolinesterase , Esterases , Resistência a Inseticidas , México , Malation/farmacologiaRESUMO
Inhabitants of urban areas are constantly exposed to light at night, which is an important environmental factor leading to circadian disruption. Streetlights filtering light through the windows and night dim light lamps are common sources of dim light at night (DLAN). The female population is susceptible to circadian disruption. The present study is aimed to determine the impact of DLAN on female Wistar rats circadian rhythms, metabolism, reproductive physiology, and behavior. After 5 weeks of DLAN exposure daily, oscillations in activity and body temperature of female rats are abolished. DLAN also decreases nocturnal food ingestion, which results in a diminishment in total food consumption. These alterations in the temporal organization of the body are associated with a significant decrease in melatonin plasmatic levels, reproductive disruptions, decreased exploration times, and marked anhedonia. This study highlights the importance of avoiding exposure to light at night, even at low intensities, to maintain the circadian organization of physiology, and denotes the great necessity of increasing the studies in females since the sexual dimorphism within the effects of desynchronizing protocols has been poorly studied.
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Atividade Motora , Fotoperíodo , Ratos , Feminino , Animais , Atividade Motora/fisiologia , Ratos Wistar , Ritmo Circadiano/fisiologia , LuzRESUMO
Knowledge of the sequencing of the 16S rRNA gene constitutes a true revolution in understanding the composition of the intestinal microbiota and its implication in health states. This study details microbial composition through next-generation sequencing (NGS) technology in children with anemia. Anemia is the most frequent hematological disorder that affects human beings. In Peru, it is one of the conditions that presents the most significant concern due to the adverse effects that cause it, such as delayed growth and psychomotor development, in addition to a deficiency in cognitive development.
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El objetivo de esta investigación fue describir la violencia estructural en la que se desarrollan las conductas disruptivas enfatizando el contexto en el que se genera el aprendizaje social de las mismas, la forma en que se construye la sociedad favoreciendo el ejercicio y prácticas de violencia que afectan a la mujer y a las personas que se desenvuelven en México. Método: 489 personas contestaron la Escala de Conductas Disruptivas en adultos; 21 de las mujeres que alguna vez fueron detenidas. Resultados: se encontró un tamaño del efecto alto en conductas disruptivas en aquellas personas que se juntaron con pandillas, el doble de consumo de sustancias psicoactivas en hombres en contraste con mujeres, así como mayores conductas disruptivas en quienes consumen sustancias ilegales o alcohol, también, el consumo de drogas y alcohol, así como la violencia vivida en la infancia presentan diferencias significativas en mujeres que han sido detenidas y aquellas que no. Discusión: los modelos de socialización, la violencia directa y estructural y la cultura de la violencia aterrizada en el contexto social mexicano generan que tanto hombres como mujeres delincan; la generación de políticas públicas y el desafío a los roles de género tradicionales pueden ser elementos protectores.
The objective of this research was to describe the structural violence in which disruptive behaviors are developed, emphasizing the context in which the social learning of these behaviors is generated, the way in which society is constructed favoring the exercise and practices of violence that affect women and people in Mexico. Method: 489 people answered the Scale of Disruptive Behavior in Adults; 21 of the women who had ever been arrested. Results: we found a high effect size in disruptive behaviors in those who joined gangs, twice the consumption of psychoactive substances in men in contrast to women, as well as greater disruptive behaviors in those who consume illegal substances or alcohol, also, the consumption of drugs and alcohol, as well as violence experienced in childhood show significant differences in women who have been arrested and those who have not. Discussion: socialization models, direct and structural violence and the culture of violence in the Mexican social context cause both men and women to commit crimes; the generation of public policies and the challenge to traditional gender roles may be protective elements.
O objetivo desta pesquisa foi descrever a violência estrutural na qual os comportamentos perturbadores são desenvolvidos, enfatizando o contexto no qual o aprendizado social destes comportamentos é gerado, a forma como a sociedade é construída favorecendo o exercício e as práticas de violência que afetam as mulheres e as pessoas no México. Método: 489 pessoas responderam à Escala de Comportamentos Perturbadores em Adultos; 21 das mulheres já haviam sido presas. Resultados: encontramos um tamanho de efeito elevado para comportamentos perturbadores naqueles que aderiram a gangues, o dobro do uso de substâncias psicoativas em homens do que em mulheres, assim como comportamentos mais perturbadores naqueles que usam substâncias ilegais ou álcool, também, o uso de drogas e álcool, assim como a violência experimentada na infância mostram diferenças significativas nas mulheres que foram presas e naquelas que não o foram. Discussão: modelos de socialização, violência direta e estrutural e a cultura da violência no contexto social mexicano levam homens e mulheres a cometer crimes; a geração de políticas públicas e o desafio aos papéis tradicionais de gênero podem ser elementos protetores.
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Humanos , Masculino , Feminino , Adulto , Violência contra a Mulher , Violência , Mulheres , MéxicoRESUMO
Objetivo: Describir las características del uso de tratamiento alternativo y complementario en dismenorrea en mujeres en edad fértil de una ciudad del norte de Perú. El estudio: Estudio trasversal analítico en mujeres en edad fértil en Mórrope, Lambayeque. Previa capacitación sobre dismenorrea y principales variables. Se entrevistó a 336 mujeres en sus domicilios previa aceptación de consentimiento informado. Hallazgos: Predominó en población estudiada el grupo etario de 15 a 35 años con 83,92% (n=282), con educación secundaria el 57,14% (n=192), más del 90% tenían estrato socioeconómico inferior/marginal. Referente a la dismenorrea, se encontró más del 30% con dolor severo/insoportable, cuyo tratamiento principal fue la MAC con 82,1%(n=220). Las plantas fueron la principal MAC, hallando solamente asociación de uso de MAC con la variable religión (p=0,023). Conclusiones: Las mujeres en edad fértil emplean notoriamente la MAC, como la fitoterapia, como tratamiento de dismenorrea a pesar de no encuentran alivio.
Objetive:To describe the characteristics of the use of alternative and complementary treatment in dysmenorrhea in women of childbearing age in a city in northern Peru. Cross-sectional analytical study in women of The study:childbearing age in Mórrope, Lambayeque. Prior training on dysmenorrhea and main variables. 336 women were interviewed in their homes after accepting informed consent. The age group of 15 to 35 years prevailed in the Findings:studied population with 83.92% (n=282), with secondary education 57.14% (n=192), more than 90% had lower/marginal socioeconomic status. Regarding dysmenorrhea, more than 30% were found to have severe/unbearable pain, whose main treatment was MAC with 82.1% (n=220). Plants were the main MAC, only finding an association of MAC use with the religion variable (p=0.023). Conclusions:Women of childbearing age notoriously use CAM, such as phytotherapy, as a treatment for dysmenorrhea despite not finding relief
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BACKGROUND: Insecticide use continues as the main strategy to control Aedes aegypti, the vector of dengue, Zika, chikungunya, and yellow fever. In the city of Tapachula, Mexico, mosquito control programs switched from pyrethroids to organophosphates for outdoor spatial spraying in 2013. Additionally, the spraying scheme switched from total coverage to focused control, prioritizing areas with higher entomological-virological risk. Five years after this strategy had been implemented, we evaluated the status and variability of insecticide resistance among Ae. aegypti collected at 26 sites in Tapachula. METHODOLOGY/PRINCIPAL FINDINGS: We determined the lethal concentrations at 50% of the tested populations (LC50) using a bottle bioassay, and then, we calculated the resistance ratio (RR) relative to the susceptible New Orleans strain. Permethrin and deltamethrin (pyrethroids), chlorpyrifos and malathion (organophosphates), and bendiocarb (carbamate) were tested. The frequencies of the substitutions V1016I and F1534C, which are in the voltage-gated sodium channel and confer knockdown-resistance (kdr) to pyrethroid insecticides, were calculated. Despite 5 years having passed since the removal of pyrethroids from the control programs, Ae. aegypti remained highly resistant to permethrin and deltamethrin (RR > 10-fold). In addition, following 5 years of chlorpyrifos use, mosquitoes at 15 of 26 sites showed moderate resistance to chlorpyrifos (5- to 10-fold), and the mosquitoes from one site were highly resistant. All sites had low resistance to malathion (< 5-fold). Resistance to bendiocarb was low at 19 sites, moderate at five, and high at two. Frequencies of the V1016I ranged from 0.16-0.71, while C1534 approached fixation at 23 sites (0.8-1). Resistance profiles and kdr allele frequencies varied across Tapachula. The variability was not associated with a spatial pattern at the scale of the sampling. CONCLUSION/SIGNIFICANCE: Mosquito populations respond to selection pressure at a focal scale in the field. Spatial variation across sites highlights the importance of testing multiple sites within geographical regions.
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Aedes/efeitos dos fármacos , Resistência a Inseticidas/genética , Inseticidas/farmacologia , Aedes/genética , Distribuição Animal , Animais , Inseticidas/classificação , México/epidemiologia , Controle de MosquitosRESUMO
La hipofisitis autoinmune es un cuadro caracterizado por la infiltración linfocítica de la hipófisis que produce deficiencia de una o más hormonas, tanto de la adenohipófisis como de la neurohipófisis. Para el diagnóstico, es necesario un alto índice de sospecha, más aún considerando la relación temporal con el embarazoo el parto. Las características clínicas e imagenológicas sugieren el diagnóstico. A pesar que el diagnóstico definitivo es por biopsia, esta no se suele realizar por los potenciales efectos adversos del procedimiento. Presentamos un caso que describe la forma de manifestación de la enfermedad y las características imagenológicas típicas en la resonancia magnética nuclear.
Autoimmune hypophysitis is a condition characterized by lymphocytic infiltration of the pituitary resulting in deficiency of one or more hormones of both the adenohypophysis and the neurohypophysis. For diagnosis, a high index of suspicion is necessary, even more so considering the temporal relationship with pregnancy or childbirth. Clinical and imaging features are suggestive of the diagnosis. Although the definitive diagnosis is by biopsy, this is not usually performed because of the potential adverse effects of the procedure. We present a case describing the form of manifestation of the disease and the typical imaging features on magnetic resonance imaging.
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The study of fitness costs of insecticide resistance mutations in Aedes aegypti has generally been focused on life history parameters such as fecundity, mortality, and energy reserves. In this study we sought to investigate whether trade-offs might also exist between insecticide resistance and other abiotic stress resistance parameters. We evaluated the effects of the selection for permethrin resistance specifically on larval salinity and thermal tolerance. A population of A. aegypti originally from Southern Mexico was split into two strains, one selected for permethrin resistance and the other not. Larvae were reared at different salinities, and the fourth instar larvae were subjected to acute thermal stress; then, survival to both stresses was compared between strains. Contrary to our predictions, we found that insecticide resistance correlated with significantly enhanced larval thermotolerance. We found no clear difference in salinity tolerance between strains. This result suggests that insecticide resistance does not necessarily carry trade-offs in all traits affecting fitness and that successful insecticide resistance management strategies must account for genetic associations between insecticide resistance and abiotic stress resistance, as well as traditional life history parameters.
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There are major public health concerns regarding the spread of mosquito-borne diseases such as dengue, Zika, and chikungunya, which are mainly controlled by using insecticides against the vectors, Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse). Pyrethroids are the primary class of insecticides used for vector control, due to their rapid knockdown effect and low toxicity to vertebrates. Unfortunately, continued use of pyrethroids has led to widespread insecticide resistance in Ae. aegypti; however, we lack information for Ae. albopictus-a sympatric species in Chiapas since 2002. In this study, we evaluated the permethrin resistance status of Ae. albopictus collected from Mexico and Texas. We also selected for permethrin resistance in the laboratory and investigated the potential mechanisms conferring resistance in this species. Knockdown resistance mutations, specifically F1534C, in the voltage-gated sodium channel gene, and increased activity of detoxifying enzymes were evaluated. Low levels of permethrin resistance (<2.4-fold) were observed in our field populations of Ae. albopictus and the F1534C mutation was not detected in any of the sites. Low levels of resistance were also observed in the artificially selected strain. There was significantly higher cytochrome P450 activity in our permethrin-selected and nonselected strains from Mexico compared to the control strain. Our results suggest the Ae. albopictus sampled from 2016 are mostly susceptible to pyrethroids. These results contrast with the high levels of permethrin resistance (>58-fold) found in Ae. aegypti from the same sites in Mexico. This research indicates the importance of continued monitoring of Ae. albopictus populations to prevent resistance from developing in the future.
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Aedes , Resistência a Inseticidas/genética , Permetrina/farmacologia , Aedes/efeitos dos fármacos , Aedes/genética , Animais , Genes de Insetos , Proteínas de Insetos/genética , Inseticidas/farmacologia , México , Controle de Mosquitos , Mosquitos Vetores/efeitos dos fármacos , Mosquitos Vetores/genética , Mutação , Piretrinas/farmacologia , Doenças Transmitidas por Vetores/prevenção & controle , Doenças Transmitidas por Vetores/transmissão , Canais de Sódio Disparados por Voltagem/genéticaRESUMO
In this study, hard corn grains were nixtamalized (alkali-heat treatment) by a high-energy ball mill to investigate the effects on its physicochemical, textural, and microstructural properties. Ball milling modifies the structure and properties of cornstarch. The gelatinization peak of starch was evidenced and thermal and pasting properties were significantly affected. With regard to rheological properties, the viscosity peak increased from 2454â¯cP in traditional nixtamalized flour to 4294â¯cP in high-energy milling treatments with 1.4% of Ca(OH)2 and 20% moisture content, C1.4, while enthalpy ranged from 3.5 to 0.34â¯J/g, respectively. High-energy milling influenced the Fourier-Transform InfraRed Spectroscopic (FT-IR) patterns. All of the samples of the corn-grain starches presented the typical A-type X-ray diffraction pattern. The crystallinity of starch from CG showed a lower intensity in peaks 2θâ¯~â¯15 and 23° compared with starch from WG and YG. The textural properties of the masas were influenced, adhesiveness was reduced, but cohesiveness was increased by the addition of Ca(OH)2. In the structural characterization by E-SEM, the control presented a greater amount of agglomerated starch granules, followed by the high-energy milling treatments. The results suggest that high-energy alkaline milling could be a potential physical and chemical method to modify corn-starch properties and obtain nixtamalized products.
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Álcalis/química , Farinha , Manipulação de Alimentos , Amido/química , Zea mays/química , Fenômenos Químicos , Manipulação de Alimentos/métodos , Termodinâmica , Viscosidade , Difração de Raios XRESUMO
Bell's Palsy is the most frequent acute neuropathy of cranial nerves; it has been associated in various reports to herpes viruses. In a prospective study we searched the presence of DNA from five herpes viruses (HSV-1 and 2, VZV, EBV and HHV-6) in 79 patients at the acute phase of Bell's Palsy. Results were related with various parameters; age, gender and clinical outcome. We found the significant presence (pË0.001) of HSV-1 and VZV in 39% and 42% of patients. However, a large percentage of cases were negative. When comparisons were made between subgroups according to gender and age no differences were found with viral findings nor with clinical outcome of palsy, which was of clinical remission in most cases (78%). Our results suggest that herpes viruses might participate in the complex mechanisms of autoimmunity of Bell's Palsy but not as determinant etiological element.
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Corticosteroides/uso terapêutico , Antivirais/uso terapêutico , Paralisia de Bell/tratamento farmacológico , Herpesvirus Humano 1/genética , Herpesvirus Humano 3/genética , Aciclovir/uso terapêutico , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antivirais/sangue , Autoimunidade , Paralisia de Bell/imunologia , Paralisia de Bell/patologia , Paralisia de Bell/virologia , Estudos de Casos e Controles , DNA Viral/sangue , DNA Viral/genética , Nervo Facial/efeitos dos fármacos , Nervo Facial/imunologia , Nervo Facial/patologia , Nervo Facial/virologia , Feminino , Herpesvirus Humano 1/patogenicidade , Herpesvirus Humano 2/genética , Herpesvirus Humano 3/patogenicidade , Herpesvirus Humano 4/genética , Herpesvirus Humano 6/genética , Humanos , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Indução de Remissão , Fatores Sexuais , Resultado do TratamentoRESUMO
Resumen: Objetivo: Evaluar la efectividad de la mezcla de flupyradifurona 26.3 g/L y transflutrina 52.5 g/L aplicada como niebla térmica a mosquitos Aedes vectores de virus dengue, Zika y chikungunya. Material y métodos: Se colocaron grupos de 15 mosquitos de Ae. aegypti (susceptibles y resistentes a piretroides) dentro de jaulas, en sala, recámara y cocina. Posteriormente, se aplicó la mezcla de flupyradifurona y transflutrina dentro de las viviendas a una dosis de 2 y 4 mg/m3, respectivamente. Resultados: La mezcla de flupyradifurona y transflutrina causó mortalidades de 97 a 100% sobre las cepas de mosquitos Aedes y su efectividad fue la misma en los diferentes compartimentos de las viviendas. Conclusiones: La mezcla de flupyradifurona y transflutrina, aplicada en niebla térmica, es una herramienta prometedora para el control de poblaciones de mosquitos Aedes independientemente de su estado de resistencia a insecticidas.
Abstract: Objective: To evaluate the efficacy of thermal fogging of a mixture of flupyrafirudone (26.3 g/L) and transfluthrin (52.5 g/L) against dengue, Zika y chikungunya Aedes mosquito vectors. Materials and methods: Groups of 15 caged Ae. aegypti (susceptible and pyrethroid resistant) mosquitoes were placed in living room, kitchen and bedroom inside houses, after which a dose of 2 and 4 mg/m3 of flupyradifurone and transfluthrine, respectively, was applied as thermal fog. After one hour of exposure mosquitoes were transferred to the laboratory and mortality was recorded after 24 h. Results: The mixture killed 97 to 100% of mosquitoes from the strains and the efficacy was similar independently of their place within the premises. Conclusions: The mixture of flupyrafirudone and transfluthrin applied as thermal fog is a promising tool to control Aedes mosquito populations independently of the pyrethroid-insecticide resistance status.
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Animais , Piridinas , 4-Butirolactona/análogos & derivados , Resistência a Inseticidas , Aedes , Ciclopropanos , Fluorbenzenos , Inseticidas , Vírus Chikungunya , Controle de Mosquitos/métodos , Aedes/virologia , Aerossóis , Vírus da Dengue , Combinação de Medicamentos , Zika virus , Mosquitos Vetores , Habitação , MéxicoRESUMO
OBJECTIVE: The Caribbean islands are regularly affected by hurricanes in a seasonal manner, but major (category 4 and 5) hurricanes are infrequent, and what happens in their aftermath is important for future planning. METHODS: We reviewed the surgical cases entered into the University of Puerto Rico (UPR) General Surgery Department database from January 1, 2016, through December 31, 2017. This database collects patient and procedural information from the surgical services of the UPR-affiliated hospitals. To analyze the impact that Hurricane Maria had on the surgical population, we compared cases per month using September 2017 (the month that Maria struck Puerto Rico) as the reference month. A chi-square goodness-of-fit test was used to evaluate differences between months. RESULTS: Information was available for 9,059 cases during the study period. The mean age of the group was 49±14 years, the gender distribution being 56% women, 44% men. The study found a statistically significant difference (p<0.001) in the number of surgical cases per month, with fewer patients in September 2017 (n = 210) compared to other months, representing a 57% decline in the number of cases. These patients tended to be sicker, with an increase in the number who had an American Society of Anesthesiologists physical status classification of 3 or greater. Within 3 months after the natural disaster, the volume of patients started to return to normal. CONCLUSION: We found that this major hurricane resulted in a decreased surgical workload, mostly because elective surgery cases were fewer, but that the patients that came for surgical care tended to be sicker and had, for the most part, poorly controlled systemic diseases. The volume of surgical cases did not reach normal levels until 3 months after the natural disaster.
Assuntos
Tempestades Ciclônicas , Centro Cirúrgico Hospitalar/estatística & dados numéricos , Procedimentos Cirúrgicos Operatórios/estatística & dados numéricos , Carga de Trabalho , Adulto , Bases de Dados Factuais , Feminino , Hospitais , Humanos , Masculino , Pessoa de Meia-Idade , Porto RicoRESUMO
OBJECTIVE: To evaluate the efficacy of thermal fogging of a mixture of flupyrafirudone (26.3 g/L) and transfluthrin (52.5 g/L) against dengue, Zika y chikungunya Aedes mosquito vectors. MATERIALS AND METHODS: Groups of 15 caged Ae. aegypti (susceptible and pyrethroid resistant) mosquitoes were placed in living room, kitchen and bedroom inside houses, after which a dose of 2 and 4 mg/m3 of flupyradifurone and transfluthrine, respectively, was applied as thermal fog. After one hour of exposure mosquitoes were transferred to the laboratory and mortality was recorded after 24 h. RESULTS: The mixture killed 97 to 100% of mosquitoes from the strains and the efficacy was similar independently of their place within the premises. CONCLUSIONS: The mixture of flupyrafirudone and transfluthrin applied as thermal fog is a promising tool to control Aedes mosquito populations independently of the pyrethroid-insecticide resistance status.
OBJETIVO: Evaluar la efectividad de la mezcla de flupyradifurona 26.3 g/L y transflutrina 52.5 g/L aplicada como niebla térmica a mosquitos Aedes vectores de virus dengue, Zika y chikungunya. MATERIAL Y MÉTODOS: Se colocaron grupos de 15 mosquitos de Ae. aegypti (susceptibles y resistentes a piretroides) dentro de jaulas, en sala, recámara y cocina. Posteriormente, se aplicó la mezcla de flupyradifurona y transflutrina dentro de las viviendas a una dosis de 2 y 4 mg/m3, respectivamente. RESULTADOS: La mezcla de flupyradifurona y transflutrina causó mortalidades de 97 a 100% sobre las cepas de mosquitos Aedes y su efectividad fue la misma en los diferentes compartimentos de las viviendas. CONCLUSIONES: La mezcla de flupyradifurona y transflutrina, aplicada en niebla térmica, es una herramienta prometedora para el control de poblaciones de mosquitos Aedes independientemente de su estado de resistencia a insecticidas.
Assuntos
4-Butirolactona/análogos & derivados , Aedes , Ciclopropanos , Fluorbenzenos , Resistência a Inseticidas , Inseticidas , Piridinas , Aedes/virologia , Aerossóis , Animais , Vírus Chikungunya , Vírus da Dengue , Combinação de Medicamentos , Habitação , México , Controle de Mosquitos/métodos , Mosquitos Vetores , Zika virusRESUMO
BACKGROUND: Resistance to pyrethroid insecticides in Aedes aegypti has become widespread after almost two decades of the frequent use of these pesticides to reduce arbovirus transmission. Despite this resistance, pyrethroids continue to be used because they are relatively inexpensive and have low human toxicity. Resistance management has been proposed as a way to retain the use of pyrethroids in natural populations. A key component of resistance management is the assumption that negative fitness is associated with resistance alleles such that resistance alleles will decline in frequency when the insecticides are removed. At least three studies in Ae. aegypti have demonstrated a decrease in pyrethroid resistance once the insecticide has been removed. METHODS/PRINCIPAL FINDINGS: The present study aims to evaluate variation in the loss of pyrethroid resistance among newly established laboratory populations of Ae. aegypti from Mexico. Eight field collections were maintained for up to eight generations, and we recorded changes in the frequencies of the mutations at the V1,016I locus and at the F1,534C locus in the voltage-gated sodium channel gene (VGSC). I1,016 and C1,534 confer resistance. We also examined resistance ratios (RR) with type 1 and 2 pyrethroids. CONCLUSIONS/SIGNIFICANCE: We demonstrate that, in general, the frequency of the Ae. aegypti pyrethroid-resistance alleles I1,016 and C1,534 decline when they are freed from pyrethroid pressure in the laboratory. However, the pattern of decline is strain dependent. In agreement with earlier studies, the RR was positively correlated with the frequencies of the resistance allele I1,016 and showed significant protection against permethrin, and deltamethrin, whereas F1,534C showed protection against permethrin but not against deltamethrin.