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A rotor design of a Ram Air Turbine (RAT) for a commercial aircraft was created taking three sections with different airfoils along the blade; those sections were assessed to evaluate their performance at different critical velocities (41, 81 and 251 m/s) and choose the best profile configuration generating a new proposal to increase the glide ratio by reducing the drag, which is helpful in emergency cases. The Blade Element Momentum (BEM) theory and Computational Fluid Dynamics (CFD) were used to analyze an initial design, then validating these results with the open software QBlade. For the BEM theory a program was created for the design and performance of the RAT adding the Viterna methodology for airfoil analysis. 16 designs were proposed by strategically interchanging wing profiles in different blade sections. These designs were analyzed by CFD, using the complete rotor and the S S T k - ω turbulence model. An optimal geometry was found, presenting a significant drag reduction of 25% generating an increase in the glide ratio and improving aircraft control in addition to maintaining the power generation above the desired values; therefore, it recommends using different airfoils for each section of a RAT's rotor blade.
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The crystallographic, optical, and electrical properties of manganese sulfide thin films depend on the control of the temperature precursors in the synthesis process, as shown by the results of this work. MnS thin films were deposited on glass substrates using the SILAR method and over an additional layer of CdS synthesized by chemical bath deposition (CBD) to acquire a p-n heterojunction. SILAR is an inexpensive method performed with a homemade robot in this case. Temperature in the solution precursors varied from 20 to 80 °C in four experiments. The morphology and structure of MnS and FTO/CdS/MnS thin films were studied through scanning electron microscopy (SEM) and grazing-incidence X-ray diffraction (GIXRD); the results indicate that materials showed a polycrystalline behavior, a diffraction peak of α- MnS cubic phase was observed with lattice constants values, ranging from 4.74 to 4.75 Å. Additionally, Raman spectroscopy showed a signal corresponding to the transversal optical phonons of MnS at a wavenumber near 300 cm-1. UV-vis spectroscopy showed optical bandgap values of 3.94, 4.0, 4.09, and 4.26 eV for thin films obtained at 20°, 40°, 60°, and 80 °C. respectively. Results indicated 80 °C as an optimal cationic precursor process temperature, achieving optical transmittance T% and good film quality according to SEM and GIXRD for the synthetization of MnS. The current-voltage (I-V) characterization in the heterojunction showed a characteristic diode curve with an open circuit voltage (VOC) of 300 mV under illumination, which indicated that the manganese sulfide behaves as p-type material contributing with positive charge carriers, while CdS behaves as n-type material.
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Characterization of bidimensional polymeric films at the air-water interface in the Langmuir trough, despite being a recurrent topic, usually refers to films of already formed polymeric materials, with very scarce reports on direct polymerization at the air-water interface. In the present work, we studied the photo-polymerization of stearyl methacrylate directly at the air-water interface under a nitrogen atmosphere, with the radical initiator solubilized in the aqueous phase. Two-dimensional (2D) polymerization was monitored by measuring the pressure-area isotherm at different irradiation times. The polymerization leads to a film with an isotherm different from that observed for the monomer, where the surface pressure is directly related to the irradiation time. The shape of this isotherm confirms the presence of a compressed liquid phase, where a higher order can be attained as a consequence of stronger packing forces involving polymer chains. The presence of inter-chain interactions allows rearrangements on the surface of the subphase, and even before the collapse a dense 2D ordering (with a solid phase-like behavior) can be observed. We present a new one-step, solvent-free procedure to obtain a photo-polymeric film directly at the air-water interface, which can be transferred to a solid surface by the Langmuir-Blodgett method, allowing film preparation of controlled thickness. Films were characterized by measuring properties such as thickness, roughness, and hydrophobicity and comparing them with films obtained from a conventional polymer. We report the differences between the interfacial behavior of amphiphilic molecules and nanomaterials such as films obtained by photo-polymerization, PSMA, directly on the air-water interface.
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The objective of the study was to identify the seroprevalence of anti-Toxoplasma gondii antibodies in sheep herds from 3 municipalities from Jalisco, Mexico, as well as estimate the association between seroprevalence and certain factors presents in the farms. In total, 12 sheep farms that maintain only hair breeds were included in the work. From these farms, 336 blood samples were collected, corresponding 324 to ewes and 12 rams. Serum samples were subjected to ELISA test, and the association between the frequency of antibodies and some potential risk factors was estimated. The overall seroprevalence to anti-T. gondii antibodies in the population studied was 17.8% (60/336; 95% confidence interval [C.I.] 14-22), all farms had positive animals, and the seroprevalence of antibodies ranged between 7 to 32%. Seroprevalence in specific municipalities was 18.7% in Lagos de Moreno, 17.8% in Encarnación de Díaz, and 16.9% in San Juan de los Lagos. Seroprevalence in ewes was 17.5% (57/324; 95% C.I. 13-22), and seroprevalence in rams was 25% (3/12; 95% C.I. 6-57), while among breeds it was 17.8% in Pelibuey (20/112; 95% C.I. 11-26), 16.6% in Kathadin (14/84; 95% C.I. 9-26), 15.4% in Blackbelly (13/84; 95% C.I. 8-25), and 23.2% in Dorper (13/56; 95% C.I. 13-36); no differences were observed among breeds (p < 0.05). The presence of cats on the farms was associated with seroprevalence (odds ratio [OR] 2.8; 95% C.I. 1.8-7.3, p < 0.001), as was the absence of a rodent-control program (OR 1.5; 95% C.I. 0.8-3.2, p < 0.05). No other factors were identified as associated with seroprevalence.
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Anticorpos Antiprotozoários/sangue , Doenças dos Ovinos/parasitologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Animais , Gatos , Feminino , Masculino , México/epidemiologia , Controle de Pragas/estatística & dados numéricos , Ratos , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/transmissão , Inquéritos e Questionários , Toxoplasmose Animal/imunologia , Toxoplasmose Animal/parasitologia , Toxoplasmose Animal/transmissãoRESUMO
The purpose of this study was to describe the prevalence and incidence of Neospora caninum infection in dogs that are in close contact with dairy cattle and to identify possible risk factors associated with the infection in this population. Twenty-four dogs located in 8 different dairy farms of Aguascalientes, Mexico, were evaluated for a 6-mo period. Once a month a sample of serum and a sample of peripheral blood was collected. The serum was used to detect antibodies against N. caninum by means of the indirect immunofluorescence technique, and the blood was used to detect parasite's DNA. The association between seroprevalence and possible risk factors was estimated using logistic regression. The prevalence of anti-N. caninum antibodies was 54% in the first month, 62% in the last month, and the incidence was 8.69%. One farm had no positive cases. Antibody titers ranged from 1:50 to 1:800. Parasite DNA was not detected in any of the samples. Only the age (>6 yr) of the dogs was identified as a risk factor for infection by N. caninum (P ≤ 0.05).
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Coccidiose/veterinária , Doenças do Cão/parasitologia , Neospora , Fatores Etários , Animais , Anticorpos Antiprotozoários/sangue , Bovinos , Coccidiose/epidemiologia , Coccidiose/parasitologia , DNA de Protozoário/sangue , DNA de Protozoário/isolamento & purificação , Indústria de Laticínios , Doenças do Cão/epidemiologia , Cães , Feminino , Incidência , Masculino , México/epidemiologia , Neospora/genética , Neospora/imunologia , Prevalência , Fatores de RiscoRESUMO
The objective of this study was to determine the serotype distribution and antibiotic resistance of invasive pneumococcal disease (IPD) strains in children from Lima, Peru, before and after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), which was introduced in the national immunisation program on 2009. We conducted a prospective, multicentre, passive surveillance IPD study during 2006-2008 and 2009-2011, before and right after the introduction of PCV7 in Peru. The study was performed in 11 hospitals and five private laboratories in Lima, Peru, in patients <18 years old, with sterile site cultures yielding Streptococcus pneumoniae. In total 159 S. pneumoniae isolates were recovered. There was a decrease in the incidence of IPD in children <2 years old after the introduction of PCV7 (18.4/100 000 vs. 5.1/100 000, P = 0.004). Meningitis cases decreased significantly in the second period (P = 0.036) as well as the overall case fatality rate (P = 0.025), including a decreased case fatality rate of pneumonia (16.3% to 0%, P = 0.04). PCV7 serotypes showed a downward trend. Vaccine-preventable serotypes caused 78.9% of IPD cases, mainly 14, 6B, 5, 19F and 23F. A non-significant increase in erythromycin resistance was reported. Our findings suggest that the introduction of PCV7 led to a significant decrease of IPD in children under 2 years old and in the overall case fatality rate.
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Farmacorresistência Bacteriana , Vacina Pneumocócica Conjugada Heptavalente/uso terapêutico , Infecções Pneumocócicas/epidemiologia , Streptococcus pneumoniae/efeitos dos fármacos , Adolescente , Fatores Etários , Criança , Pré-Escolar , Monitoramento Epidemiológico , Feminino , Humanos , Incidência , Lactente , Masculino , Peru/epidemiologia , Infecções Pneumocócicas/prevenção & controle , Estudos Prospectivos , Sorogrupo , Streptococcus pneumoniae/genética , Streptococcus pneumoniae/imunologiaRESUMO
INTRODUCCIÓN La depresión postparto ha sufrido un crecimiento significativo, pero en la sierra central peruana no existen estudios acerca de este tema. OBJETIVOS Identificar y establecer los factores de riesgo para desarrollar depresión postparto en las madres que tienen a sus hijos hospitalizados en unidades de pediatría y neonatología en la ciudad de Huancayo. MÉTODOS Estudio de casos y controles. Se incluyeron las fichas de recolección de datos de las madres que tuvieron hospitalizados a sus hijos en unidades de pediatría y neonatología durante 2017. Los casos fueron las que tuvieron "presencia de síntomas de depresión postparto". Se cruzó esta variable con otras mediante la regresión logística. Se obtuvieron los Odds ratio, sus intervalos de confianza al 95% y los valores p. RESULTADOS Se evaluaron 61 fichas de madres que padecieron depresión postparto (casos) y 61 fichas de madres sin este tipo de patología (controles). Al realizar el análisis multivariado, se encontró que aquellas madres con una condición laboral de tipo desempleada (p < 0,001), así como aquellas que fueron solteras (valor p < 0,001) y las que tuvieron un embarazo no planificado (p = 0,003); tuvieron una mayor posibilidad de depresión postparto). Por el contrario, aquellas que manifestaron tener problemas graves con su pareja tuvieron una menor oportunidad de depresión postparto (p = 0,003); ajustado por nueve variables. CONCLUSIONES Se encontraron diversos factores relacionados con la depresión postparto: el estado civil, la relación con la pareja, la condición laboral y la no planificación del embarazo.
INTRODUCTION Postpartum depression has increased significantly worldwide, but in the central Peruvian mountain, there are no studies that address this problem. OBJECTIVES To identify and to establish risk factors for postpartum depression in mothers whose children are hospitalized in pediatrics/neonatology units in Huancayo. METHODS This is a case-control study. Medical records of mothers whose children were hospitalized in pediatrics/neonatology units during 2017 were included. The cases were defined as patients who "had symptoms of postpartum depression." This variable was crossed with other variables using logistical regression, odds ratio were obtained, their confidence intervals were set to 95%, and the p values were calculated. RESULTS Sixty-one medical records of mothers with postpartum depression (cases) and 61 medical records of mothers without postpartum depression (control) were evaluated. In the multivariate analysis, the unemployed mothers had a bigger chance of postpartum depression (p < 0.001), as well as single mothers (p < 0.001), and those with an unplanned pregnancy (p = 0.003). Conversely, mothers who reported having serious problems with their partner had a smaller chance of postpartum depression (p = 0.003). CONCLUSIONS Different factors were found to be related to postpartum depression: marital situation, relationship with their partners, work conditions, and having an unplanned pregnancy.
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Humanos , Feminino , Gravidez , Recém-Nascido , Adulto , Adulto Jovem , Criança Hospitalizada/psicologia , Depressão Pós-Parto/epidemiologia , Mães/psicologia , Peru , Estudos de Casos e Controles , Fatores de Risco , Gravidez não Planejada/psicologia , Emprego/estatística & dados numéricos , Relações InterpessoaisRESUMO
Phylogenetic analysis of the rabies virus in molecular epidemiology has been traditionally performed on partial sequences of the genome, such as the N, G, and P genes; however, that approach raises concerns about the discriminatory power compared to whole genome sequencing. In this study we characterized four strains of the rabies virus isolated from cattle in Querétaro, Mexico by comparing the whole genome sequence to that of strains from the American, European and Asian continents. Four cattle brain samples positive to rabies and characterized as AgV11, genotype 1, were used in the study. A cDNA sequence was generated by reverse transcription PCR (RT-PCR) using oligo dT. cDNA samples were sequenced in an Illumina NextSeq 500 platform. The phylogenetic analysis was performed with MEGA 6.0. Minimum evolution phylogenetic trees were constructed with the Neighbor-Joining method and bootstrapped with 1000 replicates. Three large and seven small clusters were formed with the 26 sequences used. The largest cluster grouped strains from different species in South America: Brazil, and the French Guyana. The second cluster grouped five strains from Mexico. A Mexican strain reported in a different study was highly related to our four strains, suggesting common source of infection. The phylogenetic analysis shows that the type of host is different for the different regions in the American Continent; rabies is more related to bats. It was concluded that the rabies virus in central Mexico is genetically stable and that it is transmitted by the vampire bat Desmodus rotundus.
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Doenças dos Bovinos/virologia , Genoma Viral , Filogenia , Vírus da Raiva/genética , Raiva/virologia , Animais , Encéfalo , Bovinos , Doenças dos Bovinos/transmissão , Quirópteros/virologia , DNA Viral , México , Raiva/transmissão , Raiva/veterinária , Vírus da Raiva/classificação , Análise de Sequência de DNARESUMO
The anaerobic treatment of raw vinasse in a combined system consisting in two methanogenic reactors, up-flow anaerobic sludge blanket (UASB) + anaerobic packed bed reactors (APBR), was evaluated. The organic loading rate (OLR) was varied, and the best condition for the combined system was 12.5 kg COD m-3day-1 with averages of 0.289 m3 CH4 kg COD r-1for the UASB reactor and 4.4 kg COD m-3day-1 with 0.207 m3 CH4 kg COD r-1 for APBR. The OLR played a major role in the emission of H2S conducting to relatively stable quality of biogas emitted from the APBR, with H2S concentrations <10 mg L-1. The importance of the sulphate to COD ratio was demonstrated as a result of the low biogas quality recorded at the lowest ratio. It was possible to develop a proper anaerobic digestion of raw vinasse through the combined system with COD removal efficiency of 86.7% and higher CH4 and a lower H2S content in biogas.
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Biocombustíveis , Reatores Biológicos , Sulfeto de Hidrogênio/metabolismo , Metano/metabolismo , Esgotos/microbiologia , AnaerobioseRESUMO
This work considered gelatinized wheat flour fraction with properties similar to hydrocolloid to enhance the strength of dough network by improving water retention and rheological characteristics. The gelatinized (90 °C) fraction of the wheat flour was incorporated in the dough formulation at different levels (5, 10, and 20% w/w). The effects of the gelatinized flour (GF) fraction on the dough rheology and thermal properties were studied. The incorporation of GF induced a moderate increase of dough viscoelasticity and reduced the freezing and melting enthalpies. On the other hand, the changes in bread textural properties brought by incorporation of GF were insignificant, indicating that the gelatinized fraction acted as a binder that enhanced water trapping in the structure. SEM images showed a more heterogeneous crumb microstructure (e.g., gas cells, porous, etc.) bread prepared using GF. Drying kinetics obtained from TGA indicated that the water diffusivity decreased with the incorporation of GF, which suggested that the bread had a compact microstructure.
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The effect of natural mineral on the mono-digestion of maize straw was evaluated in continuously stirred tank reactors (CSTRs) at 38 °C. Different strategies of mineral addition were studied. The organic loading rate (OLR) was varied from 0.5 to 2.5 g volatile solid (VS) L(-1) d(-1). A daily addition of 1 g mineral L(-1) in reactor 2 (R2) diminished the methane production by about 11 % with respect to the initial phase. However, after a gradual addition of mineral, an average methane yield of 257 NmL CH4 g VS(-1) was reached and the methane production was enhanced by 30 % with regard to R1. An increase in the frequency of mineral addition did not enhance the methane production. The archaeal community was more sensitive to the mineral than the bacterial population whose similarity stayed high between R1 and R2. Significant difference in methane yield was found for both reactors throughout the operation.
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Reatores Biológicos , Metano/biossíntese , Zea mays , Archaea/efeitos dos fármacos , Bactérias/efeitos dos fármacos , Metano/química , Minerais/química , Minerais/farmacologia , ResíduosRESUMO
Random sequences attain the highest entropy rate. The estimation of entropy rate for an ergodic source can be done using the Lempel Ziv complexity measure yet, the exact entropy rate value is only reached in the infinite limit. We prove that typical random sequences of finite length fall short of the maximum Lempel-Ziv complexity, contrary to common belief. We discuss that, for a finite length, maximum Lempel-Ziv sequences can be built from a well defined generating algorithm, which makes them of low Kolmogorov-Chaitin complexity, quite the opposite to randomness. It will be discussed that Lempel-Ziv measure is, in this sense, less general than Kolmogorov-Chaitin complexity, as it can be fooled by an intelligent enough agent. The latter will be shown to be the case for the binary expansion of certain irrational numbers. Maximum Lempel-Ziv sequences induce a normalization that gives good estimates of entropy rate for several sources, while keeping bounded values for all sequence length, making it an alternative to other normalization schemes in use.
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A co-digestion process was evaluated when mixing different ratios of agro-industrial residues, i.e. bovine slaughterhouse waste (SB); cow manure (M); various crop residues (VC); and municipal solid waste (MSW) by anaerobic batch digestion under thermophilic conditions (55 °C). A selected study case at mesophilic condition (37 °C) was also investigated. The performance of the co-digestion was evaluated by kinetics (k(0)). The best kinetic results were obtained under thermophilic operation when a mixture of 22% w/w SB, 22% w/w M, 45% w/w VC and 11% w/w MSW was co-digested, which showed a proper combination of high values in r(s)CH(4) and k(0) (0.066 Nm(3)CH(4)/kgVS*d, 0.336 d(-1)) during the anaerobic process. The effect of temperature on methane yield (Y(CH4)), specific methane rate (r(s)CH(4)) and k(0) was also analyzed for a specific study case; there a mixture of 25% w/w of SB, 37.5% w/w of M, 37.5% of VC and 0% of MSW was used. Response variables were severely affected by mesophilic conditions, diminishing to at least 45% of the thermophilic values obtained for a similar mixture. The effect of temperature suggested that thermophilic conditions are suitable to treat these residues.
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Matadouros , Produtos Agrícolas , Resíduos Industriais , Esterco , Eliminação de Resíduos , Anaerobiose , Animais , Bovinos , TemperaturaRESUMO
Adsorption of copper and zinc in lignimerin (an organic material mainly composed by lignin, carbohydrate fragments and some extractives) and its acid derivative (H-lignimerin), recovered from Kraft cellulose mill wastewater was examined. A Box-Behnken experiment design, used to optimize lignimerin recovery process, revealed that the type of solvent used for precipitation is a determining factor in the amount of substance obtained. Conversely, batch adsorption studies at pH 4.0 revealed that the maximum adsorption capacities, modeled by the Langmuir equation, were 666.7 and 370.4 mmol kg(-1) for Cu(II) and Zn(II), respectively in lignimerin and 232.6 and 312.5 mmol kg(-1) for Cu(II) and Zn(II), respectively in H-lignimerin. The adsorption of Cu(II) and Zn(II) through deprotonated hydroxyl and carboxylic groups was the dominant mechanism that may explain the adsorption in both materials. The adsorption capacities indicated that lignimerin, with a molecular mass between 50 and 70 kDa, has a potential use as an organic sorbent for removing copper and zinc from liquid resources.
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Celulose/metabolismo , Cobre/isolamento & purificação , Resíduos Industriais , Lignina/metabolismo , Compostos Orgânicos/metabolismo , Eliminação de Resíduos Líquidos , Zinco/isolamento & purificação , Acetona , Adsorção , Biodegradação Ambiental , Centrifugação , Precipitação Química , Cinética , Soluções , Espectrofotometria Infravermelho , Propriedades de Superfície , TemperaturaRESUMO
This study evaluated the levels of soluble interleukin-2 receptor (sIL-2R) and soluble intercellular adhesion molecule-1 (sICAM-1) in patients with dengue. Sera from 17 patients with dengue fever (DF), 15 with dengue hemorrhagic fever (DHF) and 12 healthy individuals were obtained. Increased levels of sIL-2R and sICAM-1 were found in patients with DF and DHF when compared to normal; those were not correlated with leukocytes, hepatic serum enzyme levels or haemostatic parameters. Levels of sIL-2R were related to the different grades of DHF. These results suggest that increased levels of sIL-2R and sICAM-1 are a common feature of dengue.
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Molécula 1 de Adesão Intercelular/sangue , Receptores de Interleucina-2/sangue , Dengue Grave/sangue , Dengue Grave/imunologia , Adolescente , Adulto , Criança , Pré-Escolar , Estudos de Coortes , Humanos , Lactente , Molécula 1 de Adesão Intercelular/análise , Pessoa de Meia-Idade , Receptores de Interleucina-2/análiseRESUMO
Antecedentes: A comienzos de los 70, se introdujo en Chile el examen de citología cervical para la pesquisa precoz de cáncer cérvico uterino. La adherencia a este examen ha demostrado su utilidad para la pesquisa oportuna de la patología y para la disminución de la mortalidad. Objetivo: Examinar las percepciones que tiene un grupo de mujeres chilenas con lesiones preinvasoras de cuello uterino, sobre el Papanicolau. Método: Estudio descriptivo, cualitativo, transversal. Se entrevista a un grupo de 237 mujeres ingresadas al programa AUGE del Servicio de Salud Metropolitano Sur-Oriente, Santiago, Chile. Resultados: Las principales razones para no hacerse el Papanicolau y no acudir a buscar el resultado fueron: la falta de preocupación; los problemas relacionados al procedimiento como miedo, vergüenza, incomodidad y falta de tiempo; y la falta de conocimiento. Conclusión: Todas las razones dadas por las mujeres chilenas en este estudio, han sido descritas en la literatura internacional. Los profesionales de la salud están en situación de cambiar la perspectiva que las mujeres tienen y por lo tanto para mejorar la educación y la práctica del screening en las mujeres. Resultados de este estudio pueden servir de guía para desarrollar programas educativos y posteriormente ser aplicados en la práctica clínica.
Background: At the beginning of the 70s, the exam of cervical cytology was introduced in Chile. The adherence to this exam has demonstrated its utility for the opportune search of the pathology and mortality decrease. Objective: To examine perceptions about the Papanicolau (PAP) test from the perspectives of Chilean women with premalignant lesions. Method: Descriptive, qualitative, and cross-sectional study in a group of 237 women from AUGE program in the Southeast Metropolitan Public Health Service, Santiago, Chile. Results: The principal reasons for not having had a PAP test were procrastination; the problems related to the PAP test procedure like fear, embarrassment, discomfort, and lack of time; and lack of knowledge. Conclusion: All the reasons given by the Chilean women in this study have been described in the international literature. Health professionals are in a position to change the perspective that the women have, and therefore for increasing the education and cervical cancer screening for women. Results of this study can serve as a guide for education programs and can be applied in clinical practice.
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Humanos , Adulto , Feminino , Pessoa de Meia-Idade , Esfregaço Vaginal/psicologia , Conhecimentos, Atitudes e Prática em Saúde , Entrevistas como Assunto , Programas de Rastreamento , Neoplasias do Colo do Útero/prevenção & controle , Chile , Estudos Transversais , Epidemiologia Descritiva , Programas Nacionais de Saúde , Neoplasias do Colo do Útero/psicologia , Estudos de Avaliação como AssuntoRESUMO
EhADH112 is part of the EhCPADH complex, a protein involved in key events of the Entamoeba histolytica host invasion. EhADH112 participates in trophozoite adherence to target cells and in phagocytosis. We report here the finding of two EhADH112 homologues in the E. histolytica genome (EhADH112-like proteins). EhADH112 and its relatives have a Bro1 domain at their amino-terminus and a consensus context for phosphorylation by Src-tyrosine kinases, both involved in signal transduction processes in other organisms. Our findings associate EhADH112 to supplementary functions related to those reported for the Alix/AIP1 family. To elucidate the precise function of EhADH112, we studied the phenotypes displayed by trophozoites transfected with the Ehadh112 full gene. Transfected trophozoites overexpressed a 78 kDa protein, which was mainly targeted to the EhCPADH complex. Moreover, these trophozoites exhibited enhanced phagocytic rates, providing further evidence of EhADH112 contribution to adhesion and phagocytosis activities.
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Entamoeba histolytica/genética , Lectinas/fisiologia , Glicoproteínas de Membrana/fisiologia , Fagocitose/genética , Proteínas de Protozoários/fisiologia , Sequência de Aminoácidos , Animais , Western Blotting , Adesão Celular/genética , DNA de Protozoário/química , Entamoeba histolytica/fisiologia , Regulação da Expressão Gênica , Lectinas/química , Lectinas/genética , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , Plasmídeos/química , Proteínas de Protozoários/química , Proteínas de Protozoários/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , TransfecçãoRESUMO
We identified here a 576 bp rab-like gene (EhrabB) in Entamoeba histolytica. EhrabB is located 332 bp upstream from the start codon of the Ehcp112 encoding gene, but is transcribed from the complementary strand. The EhrabB open reading frame predicts a 192 amino acid polypeptide (EhRabB) with 40-42% identity to Rab proteins, involved in vesicle docking regulation in endo and exocytic pathways of eukaryotic cells. Transcripts of 0.6 and 0.97 kb were detected by the EhrabB probe in northern blot assays. Using specific antibodies, EhRabB was located in small cytoplasmic vesicles by confocal microscopy. During phagocytosis, EhRabB was initially translocated to the plasma membrane and to the phagocytic mouths. The protein diminished after 10 min phagocytosis, suggesting that EhRabB could be participating in the regulation of the endocytosis process.
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Entamoeba histolytica/genética , Entamoeba histolytica/metabolismo , Proteínas rab de Ligação ao GTP/genética , Proteínas rab de Ligação ao GTP/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Anticorpos Antiprotozoários , Transporte Biológico , Northern Blotting , Western Blotting , Membrana Celular/metabolismo , Citoplasma/metabolismo , Genes de Protozoários , Guanosina Difosfato/metabolismo , Guanosina Trifosfato/metabolismo , Microscopia Confocal , Dados de Sequência Molecular , Fagocitose , Proteínas Recombinantes/imunologia , Transcrição Gênica , Proteínas rab de Ligação ao GTP/química , Proteínas rab de Ligação ao GTP/imunologiaAssuntos
beta-Galactosidase/biossíntese , Fermentação , Indústrias , Kluyveromyces/enzimologia , LactaseRESUMO
Setenta y seis pacientes con síncope (grupo A), 26 con presíncope (grupo B) de presunto origen vasovagal y 9 sujetos controles, fueron evaluados con tilt test. Sesenta y nueve pacientes tuvieron una prueba positiva (68 por ciento), 51 de ellos luego de la administración de isoproterenol. El 80 por ciento del grupo A tuvo una prueba positiva versus 30 por ciento del grupo B (p= 0,000012) y ninguno en el grupo control. Los pacientes con síntomas recurrentes tuvieron una tasa significativamente mayor de pruebas positivas (78 por ciento versus 49 por ciento en los no recurrentes; p= 0,005). Cuatro pacientes presentaron asistolia de hasta 6,8 segundos. El tilt test es un método útil en el diagnóstico de pacientes con síncope vasovagal, especialmente en aquéllos con síntomas recurrentes (AU)