RESUMO
Biofortification aims to improve the micronutrient concentration and bioavailability in staple food crops. Unlike other strategies utilized to alleviate Fe deficiency, studies of the gut microbiota in the context of Fe biofortification are scarce. In this study, we performed a 6-week feeding trial in Gallus gallus (n = 15), aimed to investigate the Fe status and the alterations in the gut microbiome following the administration of Fe-biofortified carioca bean based diet (BC) versus a Fe-standard carioca bean based diet (SC). The tested diets were designed based on the Brazilian food consumption survey. Two primary outcomes were observed: (1) a significant increase in total body Hb-Fe values in the group receiving the Fe-biofortified carioca bean based diet; and (2) changes in the gut microbiome composition and function were observed, specifically, significant changes in phylogenetic diversity between treatment groups, as there was increased abundance of bacteria linked to phenolic catabolism, and increased abundance of beneficial SCFA-producing bacteria in the BC group. The BC group also presented a higher intestinal villi height compared to the SC group. Our results demonstrate that the Fe-biofortified carioca bean variety was able to moderately improve Fe status and to positively affect the intestinal functionality and bacterial populations.
Assuntos
Ração Animal/análise , Biofortificação , Dieta , Alimentos Fortificados , Ferro/administração & dosagem , Phaseolus/química , Fenômenos Fisiológicos da Nutrição Animal , Animais , Brasil , Células CACO-2 , Galinhas , Fibras na Dieta/análise , Proteínas Alimentares/análise , Feminino , Ferritinas/metabolismo , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos , Ferro/química , Masculino , Ácido Fítico/análise , Polifenóis/análiseRESUMO
Objective: to assess students´ acceptability and preference for cakes prepared with orange-fleshed sweet potato flour (Ipomoea batatas L.). Materials and methods: the cakes were prepared with wheat flour (sample A) and a mixture where 40% of the wheat flour was replaced by orange-fleshed sweet potato flour (sample B). Questionnaires were applied to 100 students between 10 and 12 years old from a public school in the city of Rio de Janeiro; sensory acceptance and preference tests were also applied. Results: participants were on average 11,7 years old, 80% were 6th graders, 50% were female and 97% of the students reported liking the cakes. In this group, 14% reported consuming cakes less than once a month; 27%, 1-3 times per month; 37%, 1-4 times per week; 19%, 1-2 times a day; and only 2% reported never consuming them. Both cakes had high acceptability; with an average score of 9,27 (A) and 9,26 (B) out of a maximum of 10, without any significant differences in preference. Cake consumption was part of the children´s eating habits. Conclusions: cakes prepared with 40% sweet potato flour were as well accepted as conventional cakes. Further, a portion of this cake (60 g) can contribute up to 22% of daily vitamin A needs. Key words: sweet potato (Ipomoea batatas L.), food fortified, food analysis, sensory evaluation, consumer satisfaction, food preferences.
Objetivo: evaluar la aceptabilidad y preferencia de los estudiantes por los pasteles preparados con harina de camote (Ipomoea batatas L.). Materiales y metódos:los pasteles fueron preparados con harina de trigo (muestra A) y una mezcla donde se reemplazó el 40% de la harina de trigo por harina de camote (muestra B). Se aplicaron cuestionarios a 100 estudiantes entre 10 y 12 años de edad de una escuela pública en la ciudad de Río de Janeiro, a quienes se les aplicaron pruebas sensoriales de aceptación y preferencia. Resultados:los participantes tenían en promedio 11,7 años, 80% cursaban sexto grado, 50% eran mujeres y el 97% de los estudiantes reportaron gustarles los pasteles. En este grupo, 14% informó que consumían los pasteles menos de una vez al mes, 27%, 1-3 veces al mes, 37%, 1-4 veces por semana, 19%, 1-2 veces al día y sólo un 2% informó no consumirlos. Ambos pasteles tuvieron una alta aceptación, con puntuación media de 9,27 (A) y 9,26 (B) de un máximo de 10 sin diferencias significativas en la preferencia. El consumo de pasteles fue parte de los hábitos alimentarios de estos niños. Conclusiones: pasteles elaborados con 40% de harina de camote podría tener tan buena aceptación como los convencionales. Además, una porción de este pastel (60 g) puede alcanzar hasta 22% de las necesidades diarias de vitamina A.