RESUMO
Ribotype, biotype and resistance phenotype were used to characterize 37 Acinetobacter baumannii-A. calcoaceticus complex isolates responsible for nosocomial infections in Buenos Aires. Nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. By ribotyping isolates were classified into five different clones of A. baumannii biotype 2, 3 of A. baumannii biotype 9, and 3 of Acinetobacter genospecies 13. Combination of the three epidemiological markers permitted categorization of 18 outbreak isolates into four probable strains: 2 A. baumannii biotype 2, named type I, and II, and 2 A. baumannii biotype 9. Type I (15 isolates) was the most prevalent strain at one hospital and was responsible for the outbreak. In conclusion, combined analysis of biotypes, resistance phenotypes, and ribotypes was an accurate approach for epidemiologic investigation of A. baumannii. Furthermore, ribotyping discriminated Acinetobacter genospecies 13 isolates which were phenotypically difficult to type.
Assuntos
Infecções por Acinetobacter/microbiologia , Acinetobacter/classificação , Infecção Hospitalar/microbiologia , Acinetobacter/efeitos dos fármacos , Infecções por Acinetobacter/epidemiologia , Acinetobacter calcoaceticus/classificação , Acinetobacter calcoaceticus/efeitos dos fármacos , Argentina/epidemiologia , Técnicas de Tipagem Bacteriana , Infecção Hospitalar/epidemiologia , Surtos de Doenças , Resistência Microbiana a Medicamentos , Humanos , Mapeamento por RestriçãoAssuntos
Humanos , Recém-Nascido , Infecções por Klebsiella/diagnóstico , Klebsiella pneumoniae/isolamento & purificação , Resistência Microbiana a Medicamentos/genética , Infecção Hospitalar/etiologia , Plasmídeos/isolamento & purificação , Resistência beta-Lactâmica/genética , Unidades de Terapia Intensiva Neonatal , Infecções por Klebsiella/epidemiologia , Infecções por Klebsiella/prevenção & controle , Surtos de Doenças , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Resistência Microbiana a Medicamentos/fisiologia , Plasmídeos/genética , Fezes/microbiologia , Antibacterianos , Técnicas de Tipagem BacterianaAssuntos
Humanos , Recém-Nascido , Resistência beta-Lactâmica/genética , Resistência Microbiana a Medicamentos/genética , Infecção Hospitalar/etiologia , Unidades de Terapia Intensiva Neonatal , Infecções por Klebsiella/diagnóstico , Klebsiella pneumoniae/isolamento & purificação , Plasmídeos/isolamento & purificação , Técnicas de Tipagem Bacteriana , Surtos de Doenças , Resistência Microbiana a Medicamentos/fisiologia , Fezes/microbiologia , Infecções por Klebsiella/epidemiologia , Infecções por Klebsiella/prevenção & controle , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Lactamas , Plasmídeos/genéticaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3) with a clear prevalence of HPV 6 and HPV 11 types (51.2 and 23.3 respectively). Six samples (14.0) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.(Au)
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Idoso , RESEARCH SUPPORT, NON-U.S. GOVT , Condiloma Acuminado/microbiologia , DNA Viral/análise , Doenças dos Genitais Masculinos/microbiologia , Papillomavirus Humano/genética , Infecções Tumorais por Vírus/microbiologia , Papillomavirus Humano/fisiologiaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3) with a clear prevalence of HPV 6 and HPV 11 types (51.2 and 23.3 respectively). Six samples (14.0) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Condiloma Acuminado , DNA Viral , Doenças dos Genitais Masculinos/microbiologia , Infecções Tumorais por Vírus/microbiologia , PapillomaviridaeRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3%) with a clear prevalence of HPV 6 and HPV 11 types (51.2% and 23.3% respectively). Six samples (14.0%) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6%). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.
Assuntos
Condiloma Acuminado/microbiologia , DNA Viral/análise , Doenças dos Genitais Masculinos/microbiologia , Papillomaviridae/genética , Infecções Tumorais por Vírus/microbiologia , Adulto , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/fisiologiaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3
) with a clear prevalence of HPV 6 and HPV 11 types (51.2
and 23.3
respectively). Six samples (14.0
) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6
). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.