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Cowpea (Vigna unguiculata (L.) Walp.) is a legume widely cultivated by small, medium and large producers in several Brazilian regions. However, one of the concerns for the production of cowpea in Brazil in recent years is the low rainfall activity in these regions, which generates the accumulation of salts on the surface. The objective of this work was to evaluate the effects of salt stress on growth parameters and enzyme activity in cowpea plants at different concentrations of brassinosteroids. Experiment was developed in a greenhouse using a completely randomized experimental design in a 3 x 3 factorial scheme. The treatments consisted of three levels of brassinosteroids (0, 3 and 6 µM EBL) and three levels of salt stress (0, 50 and 100 mM NaCl). Growth factors (height, diameter and number of leaves) decreased in the saline condition. With the presence of brassinosteroid the height did not increase, but the number of leaves did, mainly in the saline dosage of 100 mM NaCl. In the variable membrane integrity, brassinosteroid was efficient in both salinity dosages, the same not happening with the relative water content, where the saline condition did not affect the amount of water in the vegetable, with the application of brassino it remained high, decreasing only at dosage 100 mM NaCl. The nitrate reductase enzyme was greatly affected in the root system even with the application of increasing doses of brassino. Therefore, brassinosteroids as a promoter of saline tolerance in cowpea seedlings was positive. The concentration of 3µM of EBL provided the most satisfactory effect in tolerating the deleterious effects of the saline condition. The same cannot be concluded for the concentration of 6µM of EBL that did not promote tolerance to some variables.

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Cowpea (Vigna unguiculata (L.) Walp.) is a legume widely cultivated by small, medium and large producers in several Brazilian regions. However, one of the concerns for the production of cowpea in Brazil in recent years is the low rainfall activity in these regions, which generates the accumulation of salts on the surface. The objective of this work was to evaluate the effects of salt stress on growth parameters and enzyme activity in cowpea plants at different concentrations of brassinosteroids. Experiment was developed in a greenhouse using a completely randomized experimental design in a 3 x 3 factorial scheme. The treatments consisted of three levels of brassinosteroids (0, 3 and 6 µM EBL) and three levels of salt stress (0, 50 and 100 mM NaCl). Growth factors (height, diameter and number of leaves) decreased in the saline condition. With the presence of brassinosteroid the height did not increase, but the number of leaves did, mainly in the saline dosage of 100 mM NaCl. In the variable membrane integrity, brassinosteroid was efficient in both salinity dosages, the same not happening with the relative water content, where the saline condition did not affect the amount of water in the vegetable, with the application of brassino it remained high, decreasing only at dosage 100 mM NaCl. The nitrate reductase enzyme was greatly affected in the root system even with the application of increasing doses of brassino. Therefore, brassinosteroids as a promoter of saline tolerance in cowpea seedlings was positive. The concentration of 3µM of EBL provided the most satisfactory effect in tolerating the deleterious effects of the saline condition. The same cannot be concluded for the concentration of 6µM of EBL that did not promote tolerance to some variables.(AU)

O feijão-caupi (Vigna unguiculata (L.) Walp.) é uma leguminosa amplamente cultivada por pequenos, médios e grandes produtores em diversas regiões brasileiras. No entanto, uma das preocupações para a produção de feijão-caupi no Brasil nos últimos anos é a baixa atividade pluviométrica nessas regiões, o que gera o acúmulo de sais na superfície. O objetivo deste trabalho foi avaliar os efeitos do estresse salino nos parâmetros de crescimento e atividade enzimática em plantas de feijão-caupi em diferentes concentrações de brassinosteróides. O experimento foi desenvolvido em casa de vegetação com delineamento experimental inteiramente casualizado em esquema fatorial 3 x 3. Os tratamentos consistiram de três níveis de brassinosteróides (0, 3 e 6 µM EBL) e três níveis de estresse salino (0, 50 e 100 mM NaCl). Os fatores de crescimento (altura, diâmetro e número de folhas) diminuíram na condição salina. Com a presença do brassinosteroide a altura não aumentou, mas o número de folhas sim, principalmente na dosagem salina de 100 mM NaCl. Na variável integridade de membrana, o brassinosteróide foi eficiente em ambas as dosagens de salinidade, o mesmo não ocorrendo com o teor relativo de água, onde a condição salina não afetou a quantidade de água na hortaliça, com a aplicação de brassino manteve-se elevada, diminuindo apenas na dosagem de NaCl 100 mM. A enzima nitrato redutase foi bastante afetada no sistema radicular mesmo com a aplicação de doses crescentes de brassino. Portanto, o uso de brassinosteroides como promotor de tolerância salina em plântulas de feijão-caupi foi positivo. A concentração de 3µM de EBL proporcionou o efeito mais satisfatório em tolerar os efeitos deletérios da condição salina. O mesmo não pode ser concluído para a concentração de 6µM de EBL que não promoveu tolerância a algumas variáveis.(AU)

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The effect of geometry in the statistics of nonlinear universality classes for interface growth has been widely investigated in recent years, and it is well known to yield a split of them into subclasses. In this work, we investigate this for the linear classes of Edwards-Wilkinson and of Mullins-Herring in one and two dimensions. From comparison of analytical results with extensive numerical simulations of several discrete models belonging to these classes, as well as numerical integrations of the growth equations on substrates of fixed size (flat geometry) or expanding linearly in time (radial geometry), we verify that the height distributions (HDs) and the spatial and the temporal covariances are universal but geometry-dependent. In fact, the HDs are always Gaussian, and, when defined in terms of the so-called "KPZ ansatz" [h≃v_{∞}t+(Γt)^{ß}χ], their probability density functions P(χ) have mean null, so that all their cumulants are null, except by their variances, which assume different values in the flat and radial cases. The shape of the (rescaled) covariance curves is analyzed in detail and compared with some existing analytical results for them. Overall, these results demonstrate that the splitting of such university classes is quite general, being not restricted to the nonlinear ones.

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Circular KPZ interfaces spreading radially in the plane have Gaussian unitary ensemble (GUE) Tracy-Widom (TW) height distribution (HD) and Airy_{2} spatial covariance, but what are their statistics if they evolve on the surface of a different background space, such as a bowl, a mountain, or any surface of revolution? To give an answer to this, we report here extensive numerical analyses of several one-dimensional KPZ models on substrates whose size enlarges as 〈L(t)〉=L_{0}+ωt^{γ}, while their mean height 〈h〉 increases as usual [〈h〉∼t]. We show that the competition between the L enlargement and the correlation length (ξ≃ct^{1/z}) plays a key role in the asymptotic statistics of the interfaces. While systems with γ>1/z have HDs given by GUE and the interface width increasing as w∼t^{ß}, for γ<1/z the HDs are Gaussian, in a correlated regime where w∼t^{αγ}. For the special case γ=1/z, a continuous class of distributions exists, which interpolate between Gaussian (for small ω/c) and GUE (for ω/c≫1). Interestingly, the HD seems to agree with the Gaussian symplectic ensemble (GSE) TW distribution for ω/c≈10. Despite the GUE HDs for γ>1/z, the spatial covariances present a strong dependence on the parameters ω and γ, agreeing with Airy_{2} only for ω≫1, for a given γ, or when γ=1, for a fixed ω. These results considerably generalize our knowledge on 1D KPZ systems, unveiling the importance of the background space on their statistics.

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Recent experimental works on one-dimensional (1D) circular Kardar-Parisi-Zhang (KPZ) systems whose radii decrease in time have reported controversial conclusions about the statistics of their interfaces. Motivated by this, here we investigate several one-dimensional KPZ models on substrates whose size changes in time as L(t)=L_{0}+ωt, focusing on the case ω<0. From extensive numerical simulations, we show that for L_{0}≫1 there exists a transient regime in which the statistics is consistent with that of flat KPZ systems (the ω=0 case), for both ω<0 and ω>0. Actually, for a given model, L_{0} and |ω|, we observe that a difference between ingrowing (ω<0) and outgrowing (ω>0) systems arises only at long times (t∼t_{c}=L_{0}/|ω|), when the expanding surfaces cross over to the statistics of curved KPZ systems, whereas the shrinking ones become completely correlated. A generalization of the Family-Vicsek scaling for the roughness of ingrowing interfaces is presented. Our results demonstrate that a transient flat statistics is a general feature of systems starting with large initial sizes, regardless of their curvature. This is consistent with their recent observation in ingrowing turbulent liquid crystal interfaces, but it is in contrast with the apparent observation of curved statistics in colloidal deposition at the edge of evaporating drops. A possible explanation for this last result, as a consequence of the very small number of monolayers analyzed in this experiment, is given. This is illustrated in a competitive growth model presenting a few-monolayer transient and an asymptotic behavior consistent, respectively, with the curved and flat statistics.

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We report extensive numerical simulations of growth models belonging to the nonlinear molecular beam epitaxy (nMBE) class, on flat (fixed-size) and expanding substrates (ES). In both d=1+1 and 2+1, we find that growth regime height distributions (HDs), and spatial and temporal covariances are universal, but are dependent on the initial conditions, while the critical exponents are the same for flat and ES systems. Thus, the nMBE class does split into subclasses, as does the Kardar-Parisi-Zhang (KPZ) class. Applying the "KPZ ansatz" to nMBE models, we estimate the cumulants of the 1+1 HDs. Spatial covariance for the flat subclass is hallmarked by a minimum, which is not present in the ES one. Temporal correlations are shown to decay following well-known conjectures.

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We present a detailed study of squared local roughness (SLRDs) and local extremal height distributions (LEHDs), calculated in windows of lateral size l, for interfaces in several universality classes, in substrate dimensions d_{s}=1 and 2. We show that their cumulants follow a Family-Vicsek-type scaling, and, at early times, when ξ≪l (ξ is the correlation length), the rescaled SLRDs are given by log-normal distributions, with their nth cumulant scaling as (ξ/l)^{(n-1)d_{s}}. This gives rise to an interesting temporal scaling for such cumulants as 〈w_{n}〉_{c}∼t^{γ_{n}}, with γ_{n}=2nß+(n-1)d_{s}/z=[2n+(n-1)d_{s}/α]ß. This scaling is analytically proved for the Edwards-Wilkinson (EW) and random deposition interfaces and numerically confirmed for other classes. In general, it is featured by small corrections, and, thus, it yields exponents γ_{n} (and, consequently, α,ß and z) in good agreement with their respective universality class. Thus, it is a useful framework for numerical and experimental investigations, where it is usually hard to estimate the dynamic z and mainly the (global) roughness α exponents. The stationary (for ξ≫l) SLRDs and LEHDs of the Kardar-Parisi-Zhang (KPZ) class are also investigated, and, for some models, strong finite-size corrections are found. However, we demonstrate that good evidence of their universality can be obtained through successive extrapolations of their cumulant ratios for long times and large l. We also show that SLRDs and LEHDs are the same for flat and curved KPZ interfaces.

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Acid treatments are used in the oil and gas industry, to increase the permeability of the carbonate reservoirs by creating preferential channels, called wormholes. Channels formation is strongly influenced by acid type and injection rate. The aim of this study is to evaluate some characteristics of the microporous system of carbonate rocks, before and after acidizing. For that purpose X-ray high-resolution microtomography was used. The results show that this technique can be used as a reliable method to analyze microstructural characteristics of the wormholes.

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We show that the theoretical machinery developed for the Kardar-Parisi-Zhang (KPZ) class in low dimensions is obeyed by the restricted solid-on-solid model for substrates with dimensions up to d=6. Analyzing different restriction conditions, we show that the height distributions of the interface are universal for all investigated dimensions. It means that fluctuations are not negligible and, consequently, the system is still below the upper critical dimension at d=6. The extrapolation of the data to dimensions d≥7 predicts that the upper critical dimension of the KPZ class is infinite.

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The dynamical regimes of models belonging to the Kardar-Parisi-Zhang (KPZ) universality class are investigated in d=2+1 by extensive simulations considering flat and curved geometries. Geometry-dependent universal distributions, different from their Tracy-Widom counterpart in one dimension, were found. Distributions exhibit finite-time corrections hallmarked by a shift in the mean decaying as t(-ß), where ß is the growth exponent. Our results support a generalization of the ansatz h=v(∞)t+(Γt)(ß)χ+η+ζt(-ß) to higher dimensions, where v(∞), Γ, ζ, and η are nonuniversal quantities whereas ß and χ are universal and the last one depends on the surface geometry. Generalized Gumbel distributions provide very good fits of the distributions in at least four orders of magnitude around the peak, which can be used for comparisons with experiments. Our numerical results call for analytical approaches and experimental realizations of the KPZ class in two-dimensional systems.

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We present a numerical study of the evolution of height distributions (HDs) obtained in interface growth models belonging to the Kardar-Parisi-Zhang (KPZ) universality class. The growth is done on an initially flat substrate. The HDs obtained for all investigated models are very well fitted by the theoretically predicted Gaussian orthogonal ensemble (GOE) distribution. The first cumulant has a shift that vanishes as t(-1/3), while the cumulants of order 2≤n≤4 converge to GOE as t(-2/3) or faster, behaviors previously observed in other KPZ systems. These results yield evidences for the universality of the GOE distribution in KPZ growth on flat substrates. Finally, we further show that the surfaces are described by the Airy(1) process.

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In surfaces with grainy features, the local roughness w shows a crossover at a characteristic length r(c), with roughness exponent changing from α(1)≈1 to a smaller α(2). The grain shape, the choice of w or height-height correlation function (HHCF) C, and the procedure to calculate root-mean-square averages are shown to have remarkable effects on α(1). With grains of pyramidal shape, α(1) can be as low as 0.71, which is much lower than the previous prediction 0.85 for rounded grains. The same crossover is observed in the HHCF, but with initial exponent χ(1)≈0.5 for flat grains, while for some conical grains it may increase to χ(1)≈0.7. The universality class of the growth process determines the exponents α(2)=χ(2) after the crossover, but has no effect on the initial exponents α(1) and χ(1), supporting the geometric interpretation of their values. For all grain shapes and different definitions of surface roughness or HHCF, we still observe that the crossover length r(c) is an accurate estimate of the grain size. The exponents obtained in several recent experimental works on different materials are explained by those models, with some surface images qualitatively similar to our model films.

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Maximal- and minimal-height distributions (MAHD, MIHD) of two-dimensional interfaces grown with the nonlinear equations of Kardar-Parisi-Zhang (KPZ, second order) and of Villain-Lai-Das Sarma (VLDS, fourth order) are shown to be different. Two universal curves may be MAHD or MIHD of each class depending on the sign of the relevant nonlinear term, which is confirmed by results of several lattice models in the KPZ and VLDS classes. The difference between MAHD and MIDH is connected with the asymmetry of the local height distribution. A simple, exactly solvable deposition-erosion model is introduced to illustrate this feature. The average extremal heights scale with the same exponent of the average roughness. In contrast to other correlated systems, generalized Gumbel distributions do not fit those MAHD and MIHD, nor those of Edwards-Wilkinson growth.

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We study numerically finite-size corrections in scaling relations for roughness distributions of various interface growth models. The most common relation, which considers the average roughness as a scaling factor, is not obeyed in the steady states of a group of ballisticlike models in 2+1 dimensions, even when very large system sizes are considered. On the other hand, good collapse of the same data is obtained with a scaling relation that involves the root mean square fluctuation of the roughness, which can be explained by finite-size effects on second moments of the scaling functions. We also obtain data collapse with an alternative scaling relation that accounts for the effect of the intrinsic width, which is a constant correction term previously proposed for the scaling of . This illustrates how finite-size corrections can be obtained from roughness distributions scaling. However, we discard the usual interpretation that the intrinsic width is a consequence of high surface steps by analyzing data of restricted solid-on-solid models with various maximal height differences between neighboring columns. We also observe that large finite-size corrections in the roughness distributions are usually accompanied by huge corrections in height distributions and average local slopes, as well as in estimates of scaling exponents. The molecular-beam epitaxy model of Das Sarma and Tamborenea in 1+1 dimensions is a case example in which none of the proposed scaling relations work properly, while the other measured quantities do not converge to the expected asymptotic values. Thus although roughness distributions are clearly better than other quantities to determine the universality class of a growing system, it is not the final solution for this task.

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We study a restricted solid-on-solid model involving deposition and evaporation with probabilities p and 1 - p, respectively, in one-dimensional substrates. It presents a crossover from Edwards-Wilkinson (EW) to Kardar-Parisi-Zhang (KPZ) scaling for p approximately 0.5. The associated KPZ equation is analytically derived, exhibiting a coefficient lambda of the nonlinear term proportional to q identical with p - 1/2, which is confirmed numerically by calculation of tilt-dependent growth velocities for several values of p. This linear lambda - q relation contrasts to the apparently universal parabolic law obtained in competitive models mixing EW and KPZ components. The regions where the interface roughness shows pure EW and KPZ scaling are identified for 0.55< or =p< or =0.8, which provides numerical estimates of the crossover times tc. They scale as tc approximately lambda -phi with phi=4.1+/-0.1, which is in excellent agreement with the theoretically predicted universal value phi=4 and improves previous numerical estimates, which suggested phi approximately 3.

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Investigou-se a possibilidade de se aplicar a cefradina, metodos de analise quimica e fisico-quimica, originalmente, empregados para o controle de qualidade de penicilinas: iodometrico, peroxido de hidroenio, acidimetrico e colorimetrico da hiroxilamina. No metodo iodometrico efetuou-se um estudo comparativo para suprimir a solucao tampao. Constatou-se que na determinacao da cefradina a solucao tampao e imprescindivel para os bons resultados. O metodo do peroxido de hidrogenio mostrou-se satisfatorio.O metodo acidimetrico nao demonstrou ser conveniente para a analise da cefradina. No metodo colorimetrico da hidroxilamina realizaram-se ensaios na presenca e ausencia de niquel II. Obtiveram-se bons resultados, entretanto, nos ensaios efetuados com niquel II; a absorcao foi maior

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O metodo iodometrico requer o emprego de solucao tampao; o de peroxido de hidrogenio e satisfatorio; o colorimetrico(hidroxilamina) da bons resultados, com ou sem niquel II, embora a absorcao seja maior na presenca de niquel

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Neste trabalho apresentam-se os resultados de estudos objetivando melhorar a potencialidade do Streptomyces coeruleorubidus v. miniatomyceticus IA-4362, como produtor do complexo antibiotico miniatomicina, utilizando-se mutantes ou variantes da referida cepa, obtidas pelos metodos de mutacao natural baseado na "Population Pattern" e meios adicionados de diferentes concentracoes do proprio complexo antibiotico miniatomicina.Diferentes meios e condicoes de cultivo foram analisados

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