RESUMO
Canastra Minas Artisanal Cheese is produced in the Brazilian State of Minas Gerais using raw milk, rennet, and pingo, a natural endogenous starter culture (fermented whey) collected from the previous day's production. Due to the use of raw milk, the product can carry microorganisms that may cause foodborne diseases (FBD), including Staphylococcus aureus. Genomic characterization of S. aureus is an important tool to assess diversity, virulence, antimicrobial resistance, and the potential for causing food poisoning due to enterotoxin production. This study is aimed at exploring the genomic features of S. aureus strains isolated from Canastra Minas Artisanal Cheeses. Multilocus sequence typing (MLST) classified these strains as ST1, ST5, and a new profile ST7849 (assigned to the clonal complex CC97). These strains belonged to four spa types: t008, t127, t359, and t992. We identified antimicrobial resistance genes with phenotypic correlation against methicillin (MRSA) and tetracycline. Virulome analysis revealed genes associated with iron uptake, immune evasion, and potential capacity for adherence and biofilm formation. The toxigenic potential included cyto- and exotoxins genes, and all strains presented the genes that encode for Panton-Valentine toxin and hemolysin, and two strains encoded 4 and 8 Staphylococcal enterotoxin (SE) genes. The results revealed the pathogenic potential of the evaluated S. aureus strains circulating in the Canastra region, representing a potential risk to public health. This study also provides useful information to monitor and guide the application of control measures to the artisanal dairy food production chain.
Assuntos
Queijo , Infecções Estafilocócicas , Humanos , Staphylococcus aureus/genética , Tipagem de Sequências Multilocus , Genômica , Enterotoxinas/genéticaRESUMO
The speed of sound and attenuation are important properties for characterizing reference materials such as biological phantoms used in ultrasound applications. There are many publications on the manufacture of ultrasonic phantoms and the characterization of their properties. However, few studies have applied the principles of metrology, such as the expression of the uncertainty of measurement. The objective of this study is to validate a method for characterizing the speed of sound and the attenuation coefficient of tissue-mimicking material (TMM) based on the expression of the measurement of uncertainty. Six 60-mm-diameter TMMs were fabricated, three 10 mm thick and three 20 mm thick. The experimental setup comprised two ultrasonic transducers, acting as transmitter or receiver depending on the stage of the measurement protocol, both with a nominal center frequency of 5 MHz and an element diameter of 12.7 mm. A sine burst of 20 cycles and 20-V peak-to-peak amplitude at 5 MHz excited the transmitter transducer, producing a maximum pressure of 0.06 MPa. The measurement method was based on the through-transmission substitution immersion technique. The speed of sound measurement system was validated using a calibrated stainless-steel cylinder as reference material, and normalized errors were <0.8. The attenuation coefficient measurement method was validated using replicated measurements under repeatability conditions. The normalized error between the two measurement sets was <1. The proposed uncertainty models for the measurements of the speed of sound and the attenuation coefficient can help other laboratories develop their own uncertainty models. These validated measurement methods can be used to certify a TMM as a reference material for biotechnological applications.