RESUMO
Exercise stimulates immune responses, but the appropriate "doses" for such achievements are unsettled. Conversely, in metabolic tissues, exercise improves the heat shock (HS) response, a universal cytoprotective response to proteostasis challenges that are centred on the expression of the 70-kDa family of intracellular heat shock proteins (iHSP70), which are anti-inflammatory. Concurrently, exercise triggers the export of HSP70 towards the extracellular milieu (eHSP70), where they work as pro-inflammatory cytokines. As the HS response is severely compromised in chronic degenerative diseases of inflammatory nature, we wondered whether acute exercise bouts of different intensities could alter the HS response of lymphocytes from secondary lymphoid organs and whether this would be related to immunoinflammatory responses. Adult male Wistar rats swam for 20 min at low, moderate, high or strenuous intensities as per an overload in tail base. Controls remained at rest under the same conditions. Afterwards, mesenteric lymph node lymphocytes were assessed for the potency of the HS response (42 °C for 2 h), NF-κB binding activity, mitogen-stimulated proliferation and cytokine production. Exercise stimulated cell proliferation in an "inverted-U" fashion peaking at moderate load, which was paralleled by suppression of NF-κB activation and nuclear location, and followed by enhanced HS response in relation to non-exercised animals. Comparative levels of eHSP70 to iHSP70 (H-index) matched IL-2/IL-10 ratios. We conclude that exercise, in a workload-dependent way, stimulates immunoinflammatory performance of lymphocytes of tissues far from the circulation and this is associated with H-index of stress response, which is useful to assess training status and immunosurveillance balance.
Assuntos
Proteínas de Choque Térmico HSP70/metabolismo , Resposta ao Choque Térmico/fisiologia , Interleucina-10/metabolismo , Interleucina-2/metabolismo , Animais , Proliferação de Células , Células Cultivadas , Linfócitos/citologia , Linfócitos/metabolismo , Masculino , Microscopia Eletrônica , Microscopia de Fluorescência , NF-kappa B/metabolismo , Condicionamento Físico Animal , Ratos , Ratos Wistar , TemperaturaRESUMO
O Streptococcus agalactiae é um coco Gram-positivo, beta-hemolítico que faz parte da microbiota de membranas mucosas, colonizando, principalmente, o trato intestinal e o genitourinário. A identificação no trato anogenital das gestantes é de importância para asaúde da mulher e do feto, visto que esse microrganismo pode causar infecções neonatais graves, septicemia, pneumonia e meningite neonatal, assim como causar infecção no organismo materno e comprometer a evolução da gestação. Cento e quarenta e quatrogestantes foram submetidas à coleta de secreção vaginal e perianal para cultura em meio Stuart. A amostra da secreção vaginal foi inoculada em agar sangue e a amostra da secreção anal em meio azida, as quais foram incubadas a 37°C, por 24 horas, seguindo-se o teste de CAMP. Foi possível verificar a alta frequência de colonização por S.agalactiae, neste estudo (40%), principalmente em pacientes entre a 34ª e a 37ª semanas de gestação (64%), o que ressalta a importância da inclusão da cultura de secreção vaginal e perianal para pesquisa desse microrganismo nos exames pré-natais...
Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto Jovem , Pessoa de Meia-Idade , Membranas Extraembrionárias , Mortalidade Infantil , Gravidez , Prevalência , Streptococcus agalactiaeRESUMO
The inducible expression of the 70-kDa heat shock proteins (HSP70) is associated with homeostatically stressful situations. Stresses involving sympathetic nervous system (SNS) activation, including α1-adrenergic agonists and physical exercise, are capable of inducing HSP70 expression and release of the HSP70 inducible form, HSP72. However, whether hypoglycaemia is capable of influencing HSP70 status under a stressful situation such as insulin-induced hypoglycaemia (IIH), which also involves SNS activation, is unsettled. Hence, we decided to investigate whether the predominant signal for HSP70 expression and delivery into the blood comes from either low glucose, high insulin, or both during short-term IIH (STIIH) and long-term IIH (LTIIH). Our data indicated that low glucose level (up to 1.56 ± 0.14 mM), but not insulin, is the triggering factor responsible for a dramatic rise in HSP72 plasma concentrations (from 0.15 ± 0.01 in fed state to 0.77 ± 0.13 ng/mL during hypoglycaemic episodes). This was observed in parallel with up to 7-fold increases in interleukin-6 (IL-6) but not interleukin-10 (IL-10) or tumour necrosis factor-α (TNF-α) at STIIH. Together, the observations may suggest that HSP72 is released under hypoglycaemic conditions as a part of the homeostatic stress response, whereas at long-term, both hypoglycaemia and insulin may influence HSP72 expression in the liver, but not in kidneys. Secreted extracellular HSP72 (eHSP72) may be purely a danger signal to all the tissues of the body for the enhancement of immune and metabolic surveillance state or actively participates in glycaemic control under stressful situations.